Mostrando 821 - 840 Resultados de 989 Para Buscar '"Minions"', tiempo de consulta: 0.29s Limitar resultados
  1. 821
    “…Two low-passage (P) strains of SARS-CoV-2 (Wash1 : P4 and Munich : P1) were cultured twice in Vero E6 cells and characterized virologically. Sanger and MinION sequencing demonstrated significant deletions in the furin cleavage signal of Wash1 : P6 and minor variants in the Munich : P3 strain. …”
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    SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patients
  2. 822
    “…DNA was then barcoded with the Rapid Barcoding kit from Oxford Nanopore technologies and loaded onto a MinION flow cell. Sequencing was performed for 3 hours (intraoperative) and 24 hours (prospective). …”
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    EPCT-15. RAPID EPIGENOMIC CLASSIFICATION OF BRAIN TUMORS ENABLES INTRAOPERATIVE NEUROSURGICAL RISK MODULATION
  3. 823
    “…GOAL FOR INNOVATION: We opted to use the MinION, which is a new portable DNA sequencer that can produce data in real-time at a relatively low cost, for a NGS hands-on workshop with medical students. …”
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    Teaching next-generation sequencing to medical students with a portable sequencing device
  4. 824
    “…DNA methylation and hydroxymethylation were directly detected using a neural network model applied to the raw signal from the MinION sequencer. In contrast to prior reports, DNA methylation was negligible in mitochondria in both CpG and non-CpG contexts. …”
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    The mitochondrial genome and Epigenome of the Golden lion Tamarin from fecal DNA using Nanopore adaptive sequencing
  5. 825
    “…The complete sequences of the plasmids of the three isolates were determined by both MiSeq and MinION. The medical chart of this patient was retrospectively reviewed conducted to obtain relevant clinical information. …”
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    A transferrable IncL/M plasmid harboring a gene encoding IMP-1 metallo-β-lactamase in clinical isolates of Enterobacteriaceae
  6. 826
    “…Next, in silico phage typing was applied to twelve MinION sequenced isolates belonging to two different genetic backgrounds, namely ST117/CT24 and ST80/CT16. …”
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    Long-read sequencing-based in silico phage typing of vancomycin-resistant Enterococcus faecium
  7. 827
    “…RESULTS: We generate the first chromosome-scale, fully-phased assembly for the dikaryotic leaf rust fungus Puccinia triticina and compare Nanopore MinION and PacBio HiFi sequence-based assemblies. …”
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    Physical separation of haplotypes in dikaryons allows benchmarking of phasing accuracy in Nanopore and HiFi assemblies with Hi-C data
  8. 828
    “…WGS was performed using an Oxford MinION Mk1C instrument following the ARTIC v3 sequencing protocol. …”
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    Repeated transmission of SARS-CoV-2 in an overcrowded Irish emergency department elucidated by whole-genome sequencing
  9. 829
    “…The genome of isolate UMCG579 was sequenced using MinION (Oxford Nanopore) and MiSeq (illumina) platforms. …”
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    Case Report: Necrotizing fasciitis caused by Staphylococcus aureus positive for a new sequence variant of exfoliative toxin E
  10. 830
    “…In this study, we applied PacBio and MinION long-read sequencing to isolates of the mentioned clone C collection. …”
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    Structural genome variants of Pseudomonas aeruginosa clone C and PA14 strains
  11. 831
    “…We also tested this method on Oxford Nanopore Technologies (ONT) MinION R9.4 to assess if this method could be applied to other long-read technologies. …”
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    Highly-multiplexed and efficient long-amplicon PacBio and Nanopore sequencing of hundreds of full mitochondrial genomes
  12. 832
    “…METHODS: A novel tiled primer amplification workflow for amplifying RRV was developed with analysis using Oxford Nanopore Technology’s MinION and a custom ARTIC/InterARTIC bioinformatic protocol. …”
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    Fine-scale genomic tracking of Ross River virus using nanopore sequencing
  13. 833
    “…METHODS: In this study, we used Nanopore MinION and Illumina MiSeq approaches to sequence the whole genome of M. tuberculosis strains isolated from two deceased Asian elephants (Elephas maximus) and one human in Chitwan, Nepal. …”
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    Phylogenomic analysis supports Mycobacterium tuberculosis transmission between humans and elephants
  14. 834
    “…All isolates were sequenced by Illumina MiSeq, followed by MinION sequencing of selected organisms. Genomes were subjected to bioinformatic analysis, addressing taxonomy, clonality, phylogeny and structural characterisation of key resistance determinants within their chromosomal and plasmidic loci. …”
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    Country-wide expansion of a VIM-1 carbapenemase-producing Klebsiella oxytoca ST145 lineage in Poland, 2009–2019
  15. 835
    “…METHODS: The exon structure of BRCA1 transcripts from a previously studied control lymphoblastoid cell line were assessed using MinION nanopore sequencing of long-range reverse transcriptase-PCR amplicons. …”
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    Nanopore sequencing of full-length BRCA1 mRNA transcripts reveals co-occurrence of known exon skipping events
  16. 836
    por Naveca, Felipe Gomes, Claro, Ingra, Giovanetti, Marta, de Jesus, Jaqueline Goes, Xavier, Joilson, Iani, Felipe Campos de Melo, do Nascimento, Valdinete Alves, de Souza, Victor Costa, Silveira, Paola Paz, Lourenço, José, Santillana, Mauricio, Kraemer, Moritz U. G., Quick, Josh, Hill, Sarah C., Thézé, Julien, Carvalho, Rodrigo Dias de Oliveira, Azevedo, Vasco, Salles, Flavia Cristina da Silva, Nunes, Márcio Roberto Teixeira, Lemos, Poliana da Silva, Candido, Darlan da Silva, Pereira, Glauco de Carvalho, Oliveira, Marluce Aparecida Assunção, Meneses, Cátia Alexandra Ribeiro, Maito, Rodrigo Melo, Cunha, Claudeth Rocha Santa Brígida, Campos, Daniela Palha de Sousa, Castilho, Marcia da Costa, Siqueira, Thalita Caroline da Silva, Terra, Tiza Matos, de Albuquerque, Carlos F. Campelo, da Cruz, Laura Nogueira, de Abreu, André Luis, Martins, Divino Valerio, Simoes, Daniele Silva de Moraes Vanlume, de Aguiar, Renato Santana, Luz, Sérgio Luiz Bessa, Loman, Nicholas, Pybus, Oliver G., Sabino, Ester C., Okumoto, Osnei, Alcantara, Luiz Carlos Junior, Faria, Nuno Rodrigues
    Publicado 2019
    “…We generated 20 novel CHIKV-ECSA genomes from the Brazilian Amazon region using MinION portable genome sequencing. Phylogenetic analyses revealed that despite an early introduction of the Asian genotype in 2015 in Roraima, the large CHIKV outbreak in 2017 in Boa Vista was caused by an ECSA-lineage most likely introduced from northeastern Brazil. …”
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    Genomic, epidemiological and digital surveillance of Chikungunya virus in the Brazilian Amazon
  17. 837
    “…Doepipeline was used to optimize parameters in four use cases; 1) de-novo assembly, 2) scaffolding of a fragmented genome assembly, 3) k-mer taxonomic classification of Oxford Nanopore Technologies MinION reads, and 4) genetic variant calling. In all four cases, doepipeline found parameter settings that produced a better outcome with respect to the characteristic measured when compared to using default values. …”
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    doepipeline: a systematic approach to optimizing multi-level and multi-step data processing workflows
  18. 838
    “…Whole-genome sequencing (WGS), conjugation, and cloning were performed to identify the mechanism responsible for fosfomycin resistance. Oxford Nanopore MinION sequencing was carried out to characterize the plasmid carrying fosfomycin resistance gene and the genetic context of the novel fosA variant. …”
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    Identification of fosA10, a Novel Plasmid-Mediated Fosfomycin Resistance Gene of Klebsiella pneumoniae Origin, in Escherichia coli
  19. 839
    “…This study applied a combination of long-read Oxford Nanopore MinION and short-read Illumina MiSeq-based sequencing to obtain closed complete genome sequences of two CTX-M-producing multidrug-resistant Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway, in order to understand the potential for mobility of the detected antibiotic resistance genes (ARGs). …”
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    Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway
  20. 840
    “…Isolate relatedness was assessed using enterococcal whole-genome MLST. MinION sequencing (Oxford Nanopore) and hybrid assembly were used to resolve genetic environments/plasmids surrounding resistance genes. …”
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    Linezolid resistance in Enterococcus faecium and Enterococcus faecalis from hospitalized patients in Ireland: high prevalence of the MDR genes optrA and poxtA in isolates with diverse genetic backgrounds
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