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961por Balinandi, Stephen, Whitmer, Shannon, Mulei, Sophia, Nassuna, Charity, Pimundu, Godfrey, Muyigi, Tonny, Kainulainen, Markus, Shedroff, Elizabeth, Krapiunaya, Inna, Scholte, Florine, Nyakarahuka, Luke, Tumusiime, Alex, Kyondo, Jackson, Baluku, Jimmy, Kiconco, Jocelyn, Harris, Julie R., Ario, Alex R., Kagirita, Atek, Bosa, Henry K., Ssewanyana, Isaac, Nabadda, Susan, Mwebesa, Henry G., Aceng, Jane R., Atwine, Diana, Lutwama, Julius J., Shoemaker, Trevor R., Montgomery, Joel M., Kaleebu, Pontiano, Klena, John D.“…From the 142 confirmed cases, we performed unbiased (Illumina) and SUDV-amplicon-specific (Minion) high-throughput sequencing to obtain 120 SUDV genome-and coding-complete sequences, representing 95.4% (104/109) of SVD-confirmed individuals within a sequence-able range (Ct ≤30) and 10 genome sequences outside of this range and 6 duplicate genome sequences. …”
Publicado 2023
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962por Somerville, Vincent, Lutz, Stefanie, Schmid, Michael, Frei, Daniel, Moser, Aline, Irmler, Stefan, Frey, Jürg E., Ahrens, Christian H.“…In addition, longer Oxford Nanopore Technologies MinION reads had to be generated for one to resolve repeat regions. …”
Publicado 2019
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963por Jamal, Alainna, Brown, Kevin A, Katz, Kevin, Johnstone, Jennie, Muller, Matthew P, Allen, Vanessa, Borgia, Sergio, Boyd, David A, Ciccotelli, William, Delibasic, Kornelija, Fisman, David, Leis, Jerome, Li, Angel, Mataseje, Laura, Mehta, Mamta, Mulvey, Michael, Ng, Wil, Pantelidis, Rajni, Paterson, Aimee, McGeer, Allison“…Drain and room occupant CPE isolates underwent Illumina whole-genome and MinION sequencing. Single nucleotide variant (SNV) phlogenomic analyses and plasmid characterization were performed. …”
Publicado 2019
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964por Chan, Wai Sing, Chan, Tsun Leung, Au, Chun Hang, Leung, Chin Pang, To, Man Yan, Ng, Man Kin, Leung, Sau Man, Chan, May Kwok Mei, Ma, Edmond Shiu Kwan, Tang, Bone Siu Fai“…Briefly, HPV L1 region was amplified using PGMY and MGP primers, and PCR-positive specimens were sequenced on MinION flow cells (R9.4.1). Data generated in first 2 h were aligned with reference sequences from Papillomavirus Episteme database for genotyping. …”
Publicado 2020
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965“…Next, low-pass whole genome sequencing using a MinION ONT pocket-sized platform plus customized de-novo and reference-based workflows assembled and circularized a highly accurate mitochondrial genome in the silky shark Carcharhinus falciformis. …”
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966por Thuy, Tran Thi Dieu, Lu, Hsu-Feng, Kuo, Pei-Yun, Lin, Wei-Hung, Lin, Tzu-Ping, Lee, Yi-Tzu, Duong, Tran Thi Thuy, Wang, Ming-Cheng, Lee, Yi-Hong, Wen, Li-Li, Chen, Yu-Chen, Kao, Cheng-Yen“…The genome sequence of EC1390 was determined by Nanopore MinION MK1C platform. Conjugation assays were performed to test the transferability of EC1390 plasmids to E. coli recipient C600. …”
Publicado 2022
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967por Widiyani, Platika, Sudarwanto, Mirnawati B., Latif, Hadri, Lukman, Denny Widaya, Thong, Daniel, Rahayu, Puji“…The samples were analyzed for nitrite levels using a spectrophotometer, and the metagenomics sequencing of SFH dirt samples was performed using the MinIon nanopore method. The sequenced data were analyzed using the EPI2ME software. …”
Publicado 2022
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968“…METHODS: Transmission electron microscopy was performed to examine virion morphology. The portable MinION sequencer released by Oxford Nanopore Technologies was used to detect viral sequences in dsRNA of CX-2-infected leaf tissue. …”
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969por Palmieri, Mattia, D’Andrea, Marco Maria, Pelegrin, Andreu Coello, Mirande, Caroline, Brkic, Snezana, Cirkovic, Ivana, Goossens, Herman, Rossolini, Gian Maria, van Belkum, Alex“…Isolates belonging to ST101 harbored the carbapenemase OXA-48, which is generally encoded by an IncL/M plasmid that was no detected in our isolates. MinION sequencing was performed on a representative ST101 strain, and the obtained long reads were assembled together with the Illumina high quality reads to decipher the bla(OXA–)(48) genetic background. …”
Publicado 2020
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970por Dor, Ziv, Shnaiderman-Torban, Anat, Kondratyeva, Kira, Davidovich-Cohen, Maya, Rokney, Assaf, Steinman, Amir, Navon-Venezia, Shiri“…Whole genome sequencing (Illumina and MinION platforms) were performed for detailed phylogenetic and plasmid analyses. …”
Publicado 2020
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972por Kong, Ling Yuan, Parkes, Leighanne, Longtin, Yves, Greenaway, Christina, Zaharatos, Jerry, Loo, Vivian, Savard, Noémie, Dion, Réjean, Éric, Fournier, Roger, Michel, Moreira, Sandrine“…As part of outbreak analysis, SARS-CoV-2 isolates underwent WGS using the Oxford Nanopore Minion and the Artic V3 protocol. Lineage attribution and sequence types (ST, within 3 mutations) were assigned based on Pangolin classification and variant analysis. …”
Publicado 2021
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973por Hohl, Sarah D, Shoenbill, Kimberly A, Taylor, Kathryn L, Minion, Mara, Bates-Pappas, Gleneara E, Hayes, Rashelle B, Nolan, Margaret B, Simmons, Vani N, Steinberg, Michael B, Park, Elyse R, Ashing, Kimlin, Beneventi, Diane, Sanderson Cox, Lisa, Goldstein, Adam O, King, Andrea, Kotsen, Chris, Presant, Cary A, Sherman, Scott E, Sheffer, Christine E, Warren, Graham W, Adsit, Robert T, Bird, Jennifer E, D’Angelo, Heather, Fiore, Michael C, Van Thanh Nguyen, Claire, Pauk, Danielle, Rolland, Betsy, Rigotti, Nancy AEnlace del recurso
Publicado 2022
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974por Haraoui, Louis-Patrick, Grenier, Frédéric, Heynemand, Félix, Lévesque, Simon, Sullivan, Richard, Landecker, Hannah L, Higgins, Paul G, Rodrigue, Sébastien“…METHODS: Strains from 3 archives underwent whole-genome sequencing (Illumina NovaSeq on all, MinION on a subset) and computational analyses: assembly (Unicycler), annotation (prokka), identification (Kraken, 16S rRNA), search for carbapenemases (ResFinder, BLDB curation). …”
Publicado 2022
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975por Jamal, Alainna J, Mataseje, Laura, Brown, Kevin, Katz, Kevin, Johnstone, Jennie, Muller, Matthew, Allen, Vanessa, Borgia, Sergio, Boyd, David, Ciccotelli, William, Delibasic, Kornelija, Fisman, David, Leis, Jerome A, Li, Angel, Mehta, Mamta, Ng, Wil, Pantelidis, Rajni, Paterson, Aimee, Pikula, Gordana, Sawicki, Rachel, Schmidt, Shelley, Souto, Renata, Tang, Lin, Thomas, Cameron, McGeer, Allison, Mulvey, Michael“…We compared patient room drain CPE to prior room occupant CPE using Illumina and MinION whole-genome sequencing. RESULTS: Three-hundred and ten inpatients exposed 1,209 drains, of which 53 (4%) yielded 62 CPE isolates at 7 (70%) hospitals. …”
Publicado 2020
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976por Wyres, Kelly L., Hawkey, Jane, Mirčeta, Mirianne, Judd, Louise M., Wick, Ryan R., Gorrie, Claire L., Pratt, Nigel F., Garlick, Jill S., Watson, Kerrie M., Pilcher, David V., McGloughlin, Steve A., Abbott, Iain J., Macesic, Nenad, Spelman, Denis W., Jenney, Adam W. J., Holt, Kathryn E.“…Twenty-three isolates were selected for sequencing on the Oxford Nanopore MinION device to generate completed reference genomes (one for each ST isolated from ≥2 patients). …”
Publicado 2021
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977por Gaye, Amadou, Marcon, Yannick, Isaeva, Julia, LaFlamme, Philippe, Turner, Andrew, Jones, Elinor M, Minion, Joel, Boyd, Andrew W, Newby, Christopher J, Nuotio, Marja-Liisa, Wilson, Rebecca, Butters, Oliver, Murtagh, Barnaby, Demir, Ipek, Doiron, Dany, Giepmans, Lisette, Wallace, Susan E, Budin-Ljøsne, Isabelle, Oliver Schmidt, Carsten, Boffetta, Paolo, Boniol, Mathieu, Bota, Maria, Carter, Kim W, deKlerk, Nick, Dibben, Chris, Francis, Richard W, Hiekkalinna, Tero, Hveem, Kristian, Kvaløy, Kirsti, Millar, Sean, Perry, Ivan J, Peters, Annette, Phillips, Catherine M, Popham, Frank, Raab, Gillian, Reischl, Eva, Sheehan, Nuala, Waldenberger, Melanie, Perola, Markus, van den Heuvel, Edwin, Macleod, John, Knoppers, Bartha M, Stolk, Ronald P, Fortier, Isabel, Harris, Jennifer R, Woffenbuttel, Bruce HR, Murtagh, Madeleine J, Ferretti, Vincent, Burton, Paul REnlace del recurso
Publicado 2014
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978por Martins, Willames M. B. S., Nicolas, Marisa F., Yu, Yang, Li, Mei, Dantas, Priscila, Sands, Kirsty, Portal, Edward, Almeida, Luiz G. P., Vasconcelos, Ana Tereza R., Medeiros, Eduardo A., Toleman, Mark A., Walsh, Timothy R., Gales, Ana C., Andrey, Diego O.“…Of these, two isolates were further analyzed by Nanopore MinION sequencing, allowing complete chromosome and plasmid circularization (hybrid assembly), using Unicycler software. …”
Publicado 2020
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979por Sander, Anna-Lena, Yadouleton, Anges, Moreira-Soto, Andres, Tchibozo, Carine, Hounkanrin, Gildas, Badou, Yvette, Fischer, Carlo, Krause, Nina, Akogbeto, Petas, F. de Oliveira Filho, Edmilson, Dossou, Anges, Brünink, Sebastian, Drosten, Christian, Aïssi, Melchior A. Joël, Harouna Djingarey, Mamoudou, Hounkpatin, Benjamin, Nagel, Michael, Drexler, Jan Felix“…We decoded the target regions within the ORF1ab and N genes of the Da An dual-target kit by MinION-based amplicon sequencing. Despite relatively high similarity between SARS-CoV-2 and endemic human coronaviruses (HCoVs) within the ORF1ab target domain, no cross-detection of high-titered cell culture supernatants of HCoVs was observed, suggesting high analytical specificity. …”
Publicado 2021
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980por Holden, Diana, Sylvester, Matthew, Crandall, John, Xu, Fengfeng, Schneider, Emily C, Watson, Hillary Berman, Zhang, Peng, Bacud, Jaclyn, Mejia, Rafael, Epson, Erin, Berrada, Zenda, Mitsunaga, Tisha, Mukhopadhyay, Rituparna“…We performed whole genome sequencing (WGS) of OXA-48-like AB and other CP organisms on the Illumina MiSeq and Oxford Nanopore MinION for short and long read sequencing, respectively. …”
Publicado 2021
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