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81por Nazarenko, Maria S., Sleptcov, Aleksei A., Zarubin, Aleksei A., Salakhov, Ramil R., Shevchenko, Alexander I., Tmoyan, Narek A., Elisaphenko, Eugeny A., Zubkova, Ekaterina S., Zheltysheva, Nina V., Ezhov, Marat V., Kukharchuk, Valery V., Parfyonova, Yelena V., Zakian, Suren M., Zakharova, Irina S.Enlace del recurso
Publicado 2023
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82por Bold, Dashzeveg, Souza-Neto, Jayme A., Gombo-Ochir, Delgerzul, Gaudreault, Natasha N., Meekins, David A., McDowell, Chester D., Zayat, Batsukh, Richt, Juergen A.“…The (RT-) PCR products were then sequenced in Mongolia using the MinION nanopore portable sequencer. The resulting sequencing reads successfully identified the respective pathogens that exhibited 91–100% nucleic acid similarity to the reference strains. …”
Publicado 2023
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83“…Here we developed and optimized procedures for real-time genotyping and whole genome sequencing of PRRSV directly from clinical samples based on targeted amplicon- and long amplicon tiling sequencing using the MinION Oxford Nanopore platform. Procedures were developed and tested on 154 clinical samples (including lung, serum, oral fluid and processing fluid) with RT-PCR Ct values ranging from 15 to 35. …”
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84por Kia, Praiscillia, Katagirya, Eric, Kakembo, Fredrick Elishama, Adera, Doreen Ato, Nsubuga, Moses Luutu, Yiga, Fahim, Aloyo, Sharley Melissa, Aujat, Brendah Ronah, Anguyo, Denis Foe, Katabazi, Fred Ashaba, Kigozi, Edgar, Joloba, Moses L., Kateete, David PatrickEnlace del recurso
Publicado 2023
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85“…BACKGROUND: The MinION™ is a new, portable single-molecule sequencer developed by Oxford Nanopore Technologies. …”
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86por Giordano, Francesca, Aigrain, Louise, Quail, Michael A, Coupland, Paul, Bonfield, James K, Davies, Robert M, Tischler, German, Jackson, David K, Keane, Thomas M, Li, Jing, Yue, Jia-Xing, Liti, Gianni, Durbin, Richard, Ning, Zemin“…Long-read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base-pairs and maximum lengths reaching 100,000 base- pairs. …”
Publicado 2017
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87por Krishnakumar, Raga, Sinha, Anupama, Bird, Sara W., Jayamohan, Harikrishnan, Edwards, Harrison S., Schoeniger, Joseph S., Patel, Kamlesh D., Branda, Steven S., Bartsch, Michael S.“…Here, we have characterized the performance of the Oxford MinION nanopore sequencer for detection and evaluation of organisms with a range of genomic nucleotide bias. …”
Publicado 2018
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88por Orsini, Paola, Minervini, Crescenzio F., Cumbo, Cosimo, Anelli, Luisa, Zagaria, Antonella, Minervini, Angela, Coccaro, Nicoletta, Tota, Giuseppina, Casieri, Paola, Impera, Luciana, Parciante, Elisa, Brunetti, Claudia, Giordano, Annamaria, Specchia, Giorgina, Albano, Francesco“…We report a customized gene panel assay based on multiplex long-PCR followed by third generation sequencing on nanopore technology (MinION), designed to analyze five frequently mutated genes in chronic lymphocytic leukemia (CLL): TP53, NOTCH1, BIRC3, SF3B1 and MYD88. …”
Publicado 2018
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89por Dutreux, Fabien, Da Silva, Corinne, d’Agata, Léo, Couloux, Arnaud, Gay, Elise J., Istace, Benjamin, Lapalu, Nicolas, Lemainque, Arnaud, Linglin, Juliette, Noel, Benjamin, Wincker, Patrick, Cruaud, Corinne, Rouxel, Thierry, Balesdent, Marie-Hélène, Aury, Jean-Marc“…Nz-T4 and G12-14 genome assemblies were generated de novo and the reference JN3 genome assembly was improved using Oxford Nanopore MinION reads. The new assembly of L. biglobosa showed the existence of AT rich regions and pointed to a genome compartmentalization previously unsuspected following Illumina sequencing. …”
Publicado 2018
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90por Fraiture, Marie-Alice, Ujhelyi, Gabriella, Ovesná, Jaroslava, Van Geel, Dirk, De Keersmaecker, Sigrid, Saltykova, Assia, Papazova, Nina, Roosens, Nancy H. C.“…More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. …”
Publicado 2019
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91por Srivathsan, Amrita, Hartop, Emily, Puniamoorthy, Jayanthi, Lee, Wan Ting, Kutty, Sujatha Narayanan, Kurina, Olavi, Meier, Rudolf“…The barcodes were obtained using an improved low-cost MinION pipeline that increased the barcoding capacity sevenfold from 500 to 3500 barcodes per flowcell. …”
Publicado 2019
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92“…In this study, we evaluated the ability of Oxford Nanopore MinION direct RNA sequencing to generate a PRRSV whole genome sequence and detect and discriminate virus at the strain-level. …”
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93por Taylor, William S., Pearson, John, Miller, Allison, Schmeier, Sebastian, Frizelle, Frank A., Purcell, Rachel V.“…RESULTS: The average number of reads per sample using RNA-Sequencing was greater than 129 times that generated using MinION sequencing. However, the average read length of MinION sequences was more than 13 times that of RNA or 16S rRNA amplicon sequencing. …”
Publicado 2020
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94por Joppich, Markus, Olenchuk, Margaryta, Mayer, Julia M., Emslander, Quirin, Jimenez-Soto, Luisa F., Zimmer, Ralf“…The MinION sequencer by Oxford Nanopore Technologies turns DNA and RNA sequencing into a routine task in biology laboratories or in field research. …”
Publicado 2020
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95por Misas, Elizabeth, Gómez, Oscar M., Botero, Vanessa, Muñoz, José F., Teixeira, Marcus M., Gallo, Juan E., Clay, Oliver K., McEwen, Juan G.Enlace del recurso
Publicado 2020
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96Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinIONpor Gigante, Crystal M., Yale, Gowri, Condori, Rene Edgar, Costa, Niceta Cunha, Long, Nguyen Van, Minh, Phan Quang, Chuong, Vo Dinh, Tho, Nguyen Dang, Thanh, Nguyen Tat, Thin, Nguyen Xuan, Hanh, Nguyen Thi Hong, Wambura, Gati, Ade, Frederick, Mito, Oscar, Chuchu, Veronicah, Muturi, Mathew, Mwatondo, Athman, Hampson, Katie, Thumbi, Samuel M., Thomae, Byron G., de Paz, Victor Hugo, Meneses, Sergio, Munyua, Peninah, Moran, David, Cadena, Loren, Gibson, Andrew, Wallace, Ryan M., Pieracci, Emily G., Li, Yu“…Taken together, our evaluation highlights the MinION’s potential for low-cost, high volume sequencing of pathogens in locations with limited resources.…”
Publicado 2020
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97“…The suggested alternatives in the sample processing improved characterization of a complex skin microbiome community using MinION™ nanopore sequencing.…”
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98por Lang, Jidong, Li, Yanju, Yang, Wenjuan, Dong, Ruyi, Liang, Yuebin, Liu, Jia, Chen, Lanyou, Wang, Weiwei, Ji, Binbin, Tian, Geng, Che, Nanying, Meng, Bo“…After being sequenced by Nanopore MinION and Illumina sequencer, its entire genome was hybrid-assembled. …”
Publicado 2022
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99por de Vries, Ellen M., Cogan, Noel O. I, Gubala, Aneta J., Mee, Peter T., O’Riley, Kim J., Rodoni, Brendan C., Lynch, Stacey E.“…Due to the lack of optimal diagnostic MinION packages for Australian AIV strains the bioinformatic pipeline was specifically targeted to confidently subtype local strains. …”
Publicado 2022
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100por Azinheiro, Sarah, Roumani, Foteini, Costa-Ribeiro, Ana, Prado, Marta, Garrido-Maestu, AlejandroEnlace del recurso
Publicado 2022
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