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101por Zee, Alexander, Deng, Dori Z.Q., Adams, Matthew, Schimke, Kayla D., Corbett-Detig, Russell, Russell, Shelbi L., Zhang, Xuan, Schmitz, Robert J., Vollmers, Christopher“…Among a new generation of sequencing technologies, Oxford Nanopore Technologies (ONT) holds a unique position because the ONT MinION, an error-prone long-read sequencer, is associated with little to no capital cost. …”
Publicado 2022
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102por Cao, Minh Duc, Ganesamoorthy, Devika, Elliott, Alysha G., Zhang, Huihui, Cooper, Matthew A., Coin, Lachlan J.M.“…The recently introduced Oxford Nanopore MinION platform generates DNA sequence data in real-time. …”
Publicado 2016
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103por Lindberg, Michael R., Schmedes, Sarah E., Hewitt, F. Curtis, Haas, Jamie L., Ternus, Krista L., Kadavy, Dana R., Budowle, Bruce“…Single source and multiple donor (mixed) samples of human mitochondrial DNA were analyzed and compared using the MinION and the MiSeq platforms. A generalized variant detection strategy was employed to provide a cursory framework for evaluating the reliability and accuracy of mitochondrial sequences produced by the MinION. …”
Publicado 2016
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104por Istace, Benjamin, Friedrich, Anne, d'Agata, Léo, Faye, Sébastien, Payen, Emilie, Beluche, Odette, Caradec, Claudia, Davidas, Sabrina, Cruaud, Corinne, Liti, Gianni, Lemainque, Arnaud, Engelen, Stefan, Wincker, Patrick, Schacherer, Joseph, Aury, Jean-Marc“…Background: Oxford Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single-molecule nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of seconds. …”
Publicado 2017
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105por Jain, Miten, Tyson, John R., Loose, Matthew, Ip, Camilla L.C., Eccles, David A., O'Grady, Justin, Malla, Sunir, Leggett, Richard M., Wallerman, Ola, Jansen, Hans J., Zalunin, Vadim, Birney, Ewan, Brown, Bonnie L., Snutch, Terrance P., Olsen, Hugh E.“…Conclusions: These Phase 2 MinION experiments serve as a baseline by providing estimates for read quality, throughput, and mappability. …”
Publicado 2017
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106por Fournier, Téo, Gounot, Jean-Sébastien, Freel, Kelle, Cruaud, Corinne, Lemainque, Arnaud, Aury, Jean-Marc, Wincker, Patrick, Schacherer, Joseph, Friedrich, AnneEnlace del recurso
Publicado 2017
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107“…This study demonstrated the utilization of MinION sequencing in quick monitoring and simultaneous phylogenetic tracking of environmental ARGs to address potential health risk associated with them.…”
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108por Li, Ruichao, Xie, Miaomiao, Dong, Ning, Lin, Dachuan, Yang, Xuemei, Wong, Marcus Ho Yin, Chan, Edward Wai-Chi, Chen, Sheng“…RESULTS: Here, we demonstrate a rapid and efficient approach to obtaining multiple MDR plasmid sequences through the use of the MinION nanopore sequencing platform, which is incorporated in a portable device. …”
Publicado 2018
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109“…Here we present FusoPortal, an interactive repository of Fusobacterium genomes that were sequenced using a hybrid MinION long-read sequencing pipeline, followed by assembly and annotation using a diverse portfolio of predominantly open-source software. …”
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110por Golparian, Daniel, Donà, Valentina, Sánchez-Busó, Leonor, Foerster, Sunniva, Harris, Simon, Endimiani, Andrea, Low, Nicola, Unemo, Magnus“…However, accuracy of MinION reads has been suboptimal for many objectives and the MinION has not been evaluated for gonococci. …”
Publicado 2018
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111por Il Jun, Kang, Moon, Jangsup, Soo Kim, Taek, Kyung Kang, Chang, Mi Moon, Song, Song, Kyoung-Ho, Gyun Choe, Pyoeng, Hwan Bang, Ji, Won Park, Sang, Suk Kim, Eu, Kim, Nam-Joong, Oh, Myoung-don, Chu, Kon, Beom Park, Wan“…CONCLUSION: Nanopore sequencing of 16s rRNA gene using the MinION sequencer may be useful for identification of causing microorganism and differentiation between monobacterial and polybacterial infection when polybacterial infection is suspected. …”
Publicado 2019
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112por Acharya, Kishor, Khanal, Santosh, Pantha, Kalyan, Amatya, Niroj, Davenport, Russell J., Werner, David“…Vibrio cholerae hazards were poorly associated with plate count faecal coliforms, but flagged up by the MinION screening method, and confirmed by a qPCR assay. …”
Publicado 2019
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113“…Genomic DNA samples were prepared from five A. officinalis individuals that differ in sex and phenotypes, and sequenced with the MinION nanopore sequencer. The obtained data were 1.5–5 Gb/sample, and the average read length was larger than 1.4 kb for all the samples. …”
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114por Hong, Nguyen Thi Thu, Nghia, Ho Dang Trung, Thanh, Tran Tan, Lan, Nguyen Phu Huong, Ny, Nguyen Thi Han, Ngoc, Nghiem My, Hang, Vu Thi Ty, Chau, Le Thi My, Quynh, Van Xuan, Diem, Le Thi, Hanh, Bui Thi Bich, Hanh, Nguyen Ho Hong, Duc, Du Trong, Man, Dinh Nguyen Huy, Campbell, James, Oanh, Pham Kieu Nguyet, Day, Jeremy, Phu, Nguyen Hoan, Chau, Nguyen Van Vinh, Thwaites, Guy, Tan, Le VanEnlace del recurso
Publicado 2020
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115por Abeynayake, Shamila Weerakoon, Fiorito, Sonia, Dinsdale, Adrian, Whattam, Mark, Crowe, Bill, Sparks, Kate, Campbell, Paul Richard, Gambley, Cherie“…Here, we demonstrate a rapid in-house workflow for DNA extraction, PCR amplification of the barcode region of the mitochondrial cytochrome oxidase subunit I (COI) gene and Oxford Nanopore Technologies (ONT) MinION sequencing of amplified products multiplexed after barcoding on ONT Flongle flow cells. …”
Publicado 2021
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116por Maggiori, Catherine, Raymond-Bouchard, Isabelle, Brennan, Laura, Touchette, David, Whyte, Lyle“…We evaluated three separate approaches for MAG generation from Allen Bay, Nunavut sea ice cryoconites—HiSeq-only, MinION-only, and hybrid (HiSeq + MinION)—where field MinION sequencing yielded a reliable metagenome. …”
Publicado 2021
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117por Miura, Hiroto, Takeda, Masayuki, Yamaguchi, Megumi, Ohtani, Yoshihisa, Endo, Go, Masuda, Yasuhisa, Ito, Kaede, Nagura, Yoshio, Iwashita, Kunihiro, Mitani, Tomohiro, Suzuki, Yutaka, Kobayashi, Yasuo, Koike, Satoshi“…In experiment 3, buccal swab samples were collected from beef cattle (n = 30) and used for 16S rRNA gene amplicon sequencing using the MinION platform to validate the applicability of a combination of the MinION platform and buccal swab samples for rumen microbiota analysis. …”
Publicado 2022
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118por Koeppel, Alexander F., Goodrum, William J., Steffen, Morgan M., Wurch, Louie L., Turner, Stephen D.“…We sequenced samples drawn from before, during, and after a 2019 Microcystis harmful algal bloom (HAB) using 3rd generation sequencing with the Oxford Nanopore MinION device. We classified the eDNA reads taxonomically, and estimated the abundances of all taxa in each sample. …”
Publicado 2022
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119por Matra, Deden Derajat, Adrian, M, Karmanah, Kusuma, Jakty, Duminil, Jérôme, Sobir, Poerwanto, Roedhy“…We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). …”
Publicado 2022
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120por Sabin, Susanna, Jones, Sophie, Patel, Dhruviben, Subramaniam, Gireesh, Kelley, Julia, Aidoo, Michael, Talundzic, Eldin“…PacBio-sequenced samples with a dominant repeat-type profile from the MinION sequencing data matched the PacBio profile. …”
Publicado 2023
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