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122por Risse, Judith, Thomson, Marian, Patrick, Sheila, Blakely, Garry, Koutsovoulos, Georgios, Blaxter, Mark, Watson, Mick“…Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads. …”
Publicado 2015
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123“…BACKGROUND: The miniaturised and portable DNA sequencer MinION™ has been released to the scientific community within the framework of an early access programme to evaluate its application for a wide variety of genetic approaches. …”
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124por Imai, Kazuo, Tarumoto, Norihito, Misawa, Kazuhisa, Runtuwene, Lucky Ronald, Sakai, Jun, Hayashida, Kyoko, Eshita, Yuki, Maeda, Ryuichiro, Tuda, Josef, Murakami, Takashi, Maesaki, Shigefumi, Suzuki, Yutaka, Yamagishi, Junya, Maeda, Takuya“…Additionally, we performed amplicon sequencing of our LAMP products using MinION™ nanopore sequencer to identify each Plasmodium species. …”
Publicado 2017
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125por Imai, Kazuo, Tarumoto, Norihito, Misawa, Kazuhisa, Murakam, Takashi, Maesaki, Shigefumi, Suzuki, Yutaka, Yamagishi, Junya, Maeda, Takuya“…Finally, we performed amplicon sequencing of our LAMP methods using MinION nanopore sequencer to identify each Plasmodium species. …”
Publicado 2017
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126por Imai, Kazuo, Tamura, Kaku, Tanigaki, Tomomi, Takizawa, Mari, Nakayama, Eiko, Taniguchi, Takahiko, Okamoto, Misako, Nishiyama, Yasumasa, Tarumoto, Norihito, Mitsutake, Kotaro, Murakami, Takashi, Maesaki, Shigefumi, Maeda, Takuya“…The accuracy, recall, and precision rates of MinION sequencing were calculated by comparing the results of variant calling in the Illumina MiSeq platform and MinION sequencer. …”
Publicado 2018
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127por Kai, Shinichi, Matsuo, Yoshiyuki, Nakagawa, So, Kryukov, Kirill, Matsukawa, Shino, Tanaka, Hiromasa, Iwai, Teppei, Imanishi, Tadashi, Hirota, Kiichi“…This study establishes a relatively simple workflow for rapid bacterial identification via MinION™ sequencing, which reduces the turnaround time from sample to result, and provides a reliable method that may be applicable to clinical settings.…”
Publicado 2019
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128por Johri, Shaili, Solanki, Jitesh, Cantu, Vito Adrian, Fellows, Sam R., Edwards, Robert A., Moreno, Isabel, Vyas, Asit, Dinsdale, Elizabeth A.“…Here, we describe a Next Generation Sequencing method (using the MinION, a hand-held portable sequencing device from Oxford Nanopore Technologies), and analyses pipeline for molecular ecological studies in Chondrichthyes. …”
Publicado 2019
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129por Li, Ruichao, Xie, Miaomiao, Dong, Ning, Lin, Dachuan, Yang, Xuemei, Wong, Marcus Ho Yin, Chan, Edward Wai-Chi, Chen, ShengEnlace del recurso
Publicado 2019
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130por Rodríguez-Pérez, Héctor, Hernández-Beeftink, Tamara, Lorenzo-Salazar, José M., Roda-García, José L., Pérez-González, Carlos J., Colebrook, Marcos, Flores, Carlos“…BACKGROUND: The Oxford Nanopore Technologies (ONT) MinION portable sequencer makes it possible to use cutting-edge genomic technologies in the field and the academic classroom. …”
Publicado 2019
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131por Fauver, Joseph R., Martin, John, Weil, Gary J., Mitreva, Makedonka, Fischer, Peter U.“…Using data from a single MinION flowcell, a 90.3 Mb nuclear genome was assembled into 202 contigs with an N50 of 2.4 Mb. …”
Publicado 2019
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132por Tanaka, Hiromasa, Matsuo, Yoshiyuki, Nakagawa, So, Nishi, Kenichiro, Okamoto, Akihisa, Kai, Shinichi, Iwai, Teppei, Tabata, Yoshiteru, Tajima, Takeshi, Komatsu, Yuji, Satoh, Motohiko, Kryukov, Kirill, Imanishi, Tadashi, Hirota, KiichiEnlace del recurso
Publicado 2019
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133por d’Avila-Levy, Claudia M., Bearzatto, Bertrand, Ambroise, Jérôme, Helaers, Raphaël, Butenko, Anzhelika, Yurchenko, Vyacheslav, A. Morelli, Karina, L. C. Santos, Helena, Brouillard, Pascal, Grellier, Philippe, Gala, Jean-Luc, Vikkula, MiikkaEnlace del recurso
Publicado 2020
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134por Chan, Wai Sing, Au, Chun Hang, Chung, Yvonne, Leung, Henry Chi Ming, Ho, Dona N., Wong, Elaine Yue Ling, Lam, Tak Wah, Chan, Tsun Leung, Ma, Edmond Shiu Kwan, Tang, Bone Siu Fai“…A total of 67,082 bases across all consensus sequences were analyzed, with 67,019 bases called by both MinION and MiSeq as wildtype. For the 41 single nucleotide variants (SNVs) called by MiSeq with 100% variant allelic frequency (VAF), 39 (95.1%) were called by MinION. …”
Publicado 2020
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135“…All DNA extractions were done as per manufacturer's instructions and prepared with the same commercial ONT 16S sample preparation kit, prior to being analysed using MinION sequencing. Eight MinION 16S datasets of this microbial reference community were obtained. …”
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136por Ren, Zi-Lin, Zhang, Jia-Rong, Zhang, Xiao-Meng, Liu, Xu, Lin, Yan-Feng, Bai, Hua, Wang, Meng-Chun, Cheng, Feng, Liu, Jin-Ding, Li, Peng, Kong, Lei, Bo, Xiao-Chen, Wang, Sheng-Qi, Ni, Ming, Yan, Jiang-Wei“…The use of the MinION for forensic applications, however, is hindered by the high error rate of nanopore sequencing. …”
Publicado 2021
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137por Liu, Yuguang, Jeraldo, Patricio, Mendes-Soares, Helena, Masters, Thao, Asangba, Abigail E., Nelson, Heidi, Patel, Robin, Chia, Nicholas, Walther-Antonio, Marina“…Sequencing was performed in a hand-held Oxford Nanopore Technologies MinION sequencer. Our results show that using an in-tube amplification approach, at least 1 pg starting DNA is needed to reach the amount required for successful sequencing within 2 h. …”
Publicado 2021
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138por Tan, Choon Meng, Lin, Yu-Chen, Li, Jian-Rong, Chien, Yuan-Yu, Wang, Chien-Jui, Chou, Lin, Wang, Cheng-Wei, Chiu, Yi-Ching, Kuo, Chih-Horng, Yang, Jun-Yi“…In this study, a method that combines immunoprecipitation-based enrichment and MinION long-read DNA sequencing was developed to solve the challenge of phytoplasma genome studies. …”
Publicado 2021
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139“…In this study, interactions between the helper plasmid pSa42-91k and two common MGEs, ISEcp1 and IS15DI, which were cloned into a pBackZero-T vector, were monitored during the conjugation process to depict the molecular mechanisms underlying the plasmid fusion process mediated by insertion sequence (IS) elements. The MinION single-molecule long-read sequencing technology can dynamically reveal the plasmid recombination events and produce valuable information on genetic polymorphism and plasmid heterogeneity in different multidrug resistance (MDR) encoding bacteria. …”
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140por Komiya, Shinnosuke, Matsuo, Yoshiyuki, Nakagawa, So, Morimoto, Yoshiharu, Kryukov, Kirill, Okada, Hidetaka, Hirota, Kiichi“…METHODS: We developed a simple workflow using the nanopore sequencer MinION that allows high-resolution and rapid differentiation of vaginal microbiota. …”
Publicado 2022
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