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141por Pavlović, Jelena, Bosch-Roig, Pilar, Rusková, Magdalena, Planý, Matej, Pangallo, Domenico, Sanmartín, Patricia“…Soluble salt analysis and culture-dependent approaches combined with archaeal and bacterial 16S rRNA and fungal ITS fragment as well as with the functional genes nirK, dsr, and soxB long-amplicon MinION-based sequencing were performed. State-of-the-art technology was used for microbial identification, providing information about the microbial diversity and phylogenetic groups present and enabling us to gain some insight into the biological cycles occurring in the community key genes involved in the different geomicrobiological cycles. …”
Publicado 2022
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142por Naushad, Sohail, Mathews, Amit, Duceppe, Marc-Olivier, Kang, Mingsong, Wang, Lin Ru, Huang, HongshengEnlace del recurso
Publicado 2022
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143por Borges, Ana Sofia G., Basu, Meghna, Brinks, Erik, Bang, Corinna, Cho, Gyu-Sung, Baines, John F., Franke, Andre, Franz, Charles M. A. P.“…Sonication-based DNA extraction was performed, followed by almost complete 16S rRNA gene polymerase chain reaction amplification and MinION sequencing with the Flongle adapter. Sequence analysis was performed using NanoCLUST, while RStudio was used for graphics. …”
Publicado 2023
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144por Conti, Angela, Casagrande Pierantoni, Debora, Robert, Vincent, Corte, Laura, Cardinali, Gianluigi“…The influence of the sequencing platform (MinION and Illumina MiSeq) and the effect of different reference databases and marker sequences on metagenomic identification of species were evaluated. …”
Publicado 2022
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145por Yahya, Samar, Watson, Christopher M., Carr, Ian, McKibbin, Martin, Crinnion, Laura A., Taylor, Morag, Bonin, Hope, Fletcher, Tracy, El-Asrag, Mohammed E., Ali, Manir, Toomes, Carmel, Inglehearn, Chris F.“…A flow cell wash kit was used on a MinION flow cell to increase yield. Findings were confirmed by PacBio SMRT long-read sequencing. …”
Publicado 2023
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146por Gombert, Sara, Jahn, Kirsten, Pathak, Hansi, Burkert, Alexandra, Schmidt, Gunnar, Wiehlmann, Lutz, Davenport, Colin, Brändl, Björn, Müller, Franz-Josef, Leffler, Andreas, Deest, Maximilian, Frieling, HelgeEnlace del recurso
Publicado 2023
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147“…Oxford Nanopore Technologies recently released an USB3.0-interfaced sequencer, the MinION. To date no particular method for enriching MinION libraries has been standardized. …”
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148por Imai, Kazuo, Tarumoto, Norihito, Runtuwene, Lucky Ronald, Sakai, Jun, Hayashida, Kyoko, Eshita, Yuki, Maeda, Ryuichiro, Tuda, Josef, Ohno, Hideaki, Murakami, Takashi, Maesaki, Shigefumi, Suzuki, Yutaka, Yamagishi, Junya, Maeda, Takuya“…The C580Y mutation was identified based on the sequence data collected from MinION reads 30 min after the start of sequencing. …”
Publicado 2018
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149por Elliott, Ivo, Batty, Elizabeth M., Ming, Damien, Robinson, Matthew T., Nawtaisong, Pruksa, de Cesare, Mariateresa, Newton, Paul N., Bowden, Rory“…The infrastructure challenges and costs of next-generation sequencing have been largely overcome, for many sequencing applications, by Oxford Nanopore Technologies’ portable MinION sequencer. However, the question remains open whether MinION-based bacterial whole genome sequencing is by itself sufficient for the accurate assessment of phylogenetic and epidemiological relationships between isolates and whether such tasks can be undertaken in resource-limited settings. …”
Publicado 2020
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150por Butt, Salman L., Erwood, Eric C., Zhang, Jian, Sellers, Holly S., Young, Kelsey, Lahmers, Kevin K., Stanton, James B.“…Amplicons were barcoded to allow for pooling of samples, processed per manufacturer’s instructions into a 1D MinION sequencing library, and then sequenced on the MinION. …”
Publicado 2020
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151por Chen, Zhao, Kuang, Dai, Xu, Xuebin, González-Escalona, Narjol, Erickson, David L., Brown, Eric, Meng, Jianghong“…The MinION reads were base-called in real-time using MinKnow 3.4.8 integrated with Guppy 3.0.7. …”
Publicado 2020
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152por Yang, Wenjuan, Liu, Ying, Dong, Ruyi, Liu, Jia, Lang, Jidong, Yang, Jialiang, Wang, Weiwei, Li, Jingjing, Meng, Bo, Tian, GengEnlace del recurso
Publicado 2020
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153“…METHODS: We have developed a method using Oxford Nanopore MinION long-read sequencing and universal eukaryotic primers to produce full-length (> 1800 bp) SSU rRNA gene sequences for Blastocystis. …”
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154por Krasnov, George, Shkrigunov, Timur, Radko, Sergey, Ptitsyn, Konstantin, Shapovalova, Valeriya, Timoshenko, Olga, Khmeleva, Svetlana, Kurbatov, Leonid, Kiseleva, Yana, Ilgisonis, Ekaterina, Kiseleva, Olga, Vakhrushev, Igor, Tsvetkova, Anastasia, Buromski, Ivan, Markin, Sergey, Archakov, Alexander, Lisitsa, Andrey, Ponomarenko, Elena“…The data of high-throughput sequencing are provided as Excel spreadsheets, where the data on FPKM and TMP values were evaluated for the whole transcriptome with both Illumina HiSeq and Oxford Nanopore Technologies MinION sequencing.…”
Publicado 2021
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155por Takashima, Tomoya, Brisset, Sophie, Furukawa, Asuka, Taniguchi, Hirohisa, Takeyasu, Rika, Kawamura, Akio, Tamura, Yuichi“…This study aimed to determine the feasibility of using MinION nanopore sequencing for the genetic analysis of PAH patients, focused on BMPR2. …”
Publicado 2021
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156por Tabata, Yoshiteru, Matsuo, Yoshiyuki, Fujii, Yosuke, Ohta, Atsufumi, Hirota, KiichiEnlace del recurso
Publicado 2022
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157por Tarquini, Giulia, Martini, Marta, Maestri, Simone, Firrao, Giuseppe, Ermacora, Paolo“…In conclusion, this work sheds light on the cause of the severe diseases affecting the ‘Lamon bean’ by exploitation of MinION sequencing.…”
Publicado 2022
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158“…Yet the long reads produced by MinION (~2900 bp) allowed accurate phylogenetic reconstruction studies. …”
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159por Maes, Mailis, Khokhar, Fahad, Wilkinson, Sam A. J., Smith, Andrew D., Kovalenko, Ganna, Dougan, Gordon, Quick, Joshua, Loman, Nicholas J., Baker, Stephen, Curran, Martin D., Skittrall, Jordan P., Houldcroft, Charlotte J.Enlace del recurso
Publicado 2023
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160“…The miniaturized and portable DNA sequencer MinION™ has demonstrated great potential in different analyses such as genome-wide sequencing, pathogen outbreak detection and surveillance, human genome variability, and microbial diversity. …”
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