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2021por Li, Mingjun, Li, Changchang, Jiang, Kairong, Li, Ke, Zhang, Junlei, Sun, Miao, Wu, Gentu, Qing, Ling“…Potato virus X-mediated heteroexpression of TbCSV V2 in Nicotiana benthamiana induced hypersensitive response-like (HR-like) cell death and systemic necrosis in a manner independent of V2′s suppressor activity. …”
Publicado 2021
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2022“…Here, we report a multipurpose plant rhabdovirus-based vector system suitable for a wide range of applications in Nicotiana benthamiana. We engineered sonchus yellow net rhabdovirus (SYNV)-based gene silencing vectors through expressing a sense, antisense, or double-stranded RNAs of target genes. …”
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2023“…LsPDI1 and its orthologs were highly conserved among various planthopper species and strongly induced ROS burst and cell death in plants. Transient in Nicotiana benthamiana LsPDI1 expression impaired the performance of Spodoptera frugiperda and Myzus persicae on host plants. …”
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2024por Dunker, Florian, Oberkofler, Lorenz, Lederer, Bernhard, Trutzenberg, Adriana, Weiberg, Arne“…We functionally characterized HaCR1 by ectopic expression in Nicotiana benthamiana. HaCR1 was capable of inhibiting effector-triggered plant cell death. …”
Publicado 2020
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2025Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistancepor Zhu, Dandan, Zhang, Xiangyue, Zhou, Jinglong, Wu, Yajie, Zhang, Xiaojian, Feng, Zili, Wei, Feng, Zhao, Lihong, Zhang, Yalin, Shi, Yongqiang, Feng, Hongjie, Zhu, Heqin“…Subcellular localization in Nicotiana benthamiana showed that GhRPS6 was localized in the nucleus. …”
Publicado 2021
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2026por Grützner, Ramona, Martin, Patrick, Horn, Claudia, Mortensen, Samuel, Cram, Erin J., Lee-Parsons, Carolyn W.T., Stuttmann, Johannes, Marillonnet, Sylvestre“…The intronized Cas9 gene was also found to be effective in other plants such as Nicotiana benthamiana and Catharanthus roseus.…”
Publicado 2020
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2027por Li, Chenyang, Xu, Yi, Fu, Shuai, Liu, Yu, Li, Zongdi, Zhang, Tianze, Wu, Jianxiang, Zhou, Xueping“…Here, we show that rice stripe virus (RSV) elicits the UPR in Nicotiana benthamiana. The RSV-induced UPR activates the host autophagy pathway by which the RSV-encoded movement protein, NSvc4, is targeted for autophagic degradation. …”
Publicado 2021
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2028por Li, Yu, Jiao, Mengting, Li, Yingjuan, Zhong, Yu, Li, Xiaoqin, Chen, Zhuangzhuang, Chen, Suiyun, Wang, Jianguang“…To test our hypothesis, we successfully generated a Nicotiana benthamiana ABA biosynthesis mutant and evaluated the role of the ABA pathway in PDMP-induced callose deposition during resistance to TMV infection. …”
Publicado 2021
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2029por Park, Youngmin, Oh, Yeonsu, Wang, Miaomiao, Ganges, Llilianne, Bohórquez, José Alejandro, Park, Soohong, Gu, Sungmin, Park, Jungae, Lee, Sangmin, Kim, Jongkook, Sohn, Eun-Ju“…The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. …”
Publicado 2021
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2030“…Using a green fluorescent protein (GFP) movement assay in the epidermis of Nicotiana benthamiana, we have shown that PD transport significantly increases when leaves are supplied with exogenous cytokinins at physiologically relevant concentrations or when a positive regulator of cytokinin responses, ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 5 (AHP5), is overexpressed. …”
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2031por Zhang, Xiaoxia, Huang, Huoqing, Wu, Bangting, Xie, Jianghui, Viljoen, Altus, Wang, Wei, Mostert, Diane, Xie, Yanling, Fu, Gang, Xiang, Dandan, Lyu, Shuxia, Liu, Siwen, Li, Chunyu“…FocM35_1 induced cell death in Nicotiana benthamiana while suppressing the INF1-induced hypersensitive response (HR), and its predicted enzymatic site was required for lesion formation and the suppression to INF1-induced HR on N. benthamiana leaves. …”
Publicado 2021
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2032por Koeduka, Takao, Takarada, Sachiho, Fujii, Koya, Sugiyama, Akifumi, Yazaki, Kazufumi, Nishihara, Masahiro, Matsui, Kenji“…This shift led to the efficient and stable production of raspberry ketone and its glycosides via heterologous expression of the biosynthetic enzymes benzalacetone synthase (BAS) and raspberry ketone/zingerone synthase 1 (RZS1) in the transgenic tobacco (Nicotiana tabacum ‘Petit Havana SR-1’). Additionally, we achieved improved product titers by activating the phenylpropanoid pathway with the transcriptional factor, production of anthocyanin pigment 1 (PAP1), from Arabidopsis thaliana. …”
Publicado 2021
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2033“…However, the infectivity of the clones carrying the HDV ribozyme in Nicotiana benthamiana plants increased, on average, by two-fold compared with the previously available clones.…”
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2034por Sami, Amel, Elimairi, Imad, Patangia, Dhrati, Watkins, Claire, Ryan, C. Anthony, Ross, R. Paul, Stanton, Catherine“…Toombak is a smokeless tobacco produced from the Nicotiana rustica tobacco plant from Sudan. Pre-prepared and ready to buy Toombak samples were analysed using mass spectrometry (heavy metals), gas and liquid chromatography (metabolomics), 16S rRNA metagenomic sequencing (microbiome) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) and pH analysis. …”
Publicado 2021
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2035por Liu, Lisen, Wang, Zhaohan, Li, Jianing, Wang, Ye, Yuan, Jiachen, Zhan, Jingjing, Wang, Peng, Lin, Yongjun, Li, Fuguang, Ge, Xiaoyang“…The transient expression of Vd424Y in Nicotiana benthamiana induced BAK1‐ and SOBIR1‐dependent cell death and activated both salicylic acid and jasmonic acid signalling. …”
Publicado 2021
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2036Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum“…Here, we sought to identify specific phenolic exudates in tobacco (Nicotiana tobaccum) plants infected by the soil-borne pathogen Ralstonia solanacearum that may exhibit antibacterial activity and promote plant resistance against pathogens. …”
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2037por Lee, Shu-Chuan, Pai, Hsuan, Huang, Ying-Wen, He, Meng-Hsun, Song, Yun-Lin, Kuo, Song-Yi, Chang, Wen-Chi, Hsu, Yau-Heiu, Lin, Na-Sheng“…In order to reveal the underlying mechanisms, we generated infectious cDNA clones of ORSV and CymMV isolated from Phalaenopsis that exerted similar unilateral synergism in both Phalaenopsis orchid and Nicotiana benthamiana. Moreover, we show that the ORSV replicase P126 is a VSR. …”
Publicado 2021
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2038por Cheng, De-Jie, Xu, Xiao-Jie, Yan, Zhi-Yong, Tettey, Carlos Kwesi, Fang, Le, Yang, Guang-Ling, Geng, Chao, Tian, Yan-Ping, Li, Xiang-Dong“…In this study, we observed that Nicotiana benthamiana ribosomal protein large subunit 1 (NbRPL1), a chloroplast ribosomal protein, localized to the chloroplasts via its N-terminal 61 amino acids (transit peptide), and interacted with tobacco vein banding mosaic virus (TVBMV) nuclear inclusion protein b (NIb), an RNA-dependent RNA polymerase. …”
Publicado 2021
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2039por Feng, Zili, Zheng, Fenghua, Wu, Silin, Li, Rui, Li, Yue, Zhong, Jiaxin, Zhao, Hongbo“…Recombinant CsVI1 proteins purified from Pichia pastoris and Nicotiana benthamiana leaves could hydrolyze sucrose into hexoses. …”
Publicado 2021
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2040por Tsushima, Ayako, Narusaka, Mari, Gan, Pamela, Kumakura, Naoyoshi, Hiroyama, Ryoko, Kato, Naoki, Takahashi, Shunji, Takano, Yoshitaka, Narusaka, Yoshihiro, Shirasu, Ken“…Transient expression assays showed that one of these putative conserved effectors, CEC3, induces nuclear expansion and cell death in Nicotiana benthamiana, suggesting that CEC3 is involved in promoting host cell death during infection. …”
Publicado 2021
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