“…The EpCAM protein fused to the fragment crystallizable region of immunoglobulin G (IgG) tagged with KDEL endoplasmic reticulum (ER) retention signal (EpCAM-FcK) has been successfully expressed in transgenic tobacco (Nicotiana tabacum cv. Xanthi) and purified from the plant leaf. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Q-Griffithsin (Q-GRFT) is a red algal lectin, manufactured in Nicotiana benthamiana. Griffithsin has well characterized broad spectrum antiviral activity and has demonstrated potent in vitro activity against multiple strains of Candida, including C. albicans. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The expression of RXLR242 was up‐regulated during infection, and its ectopic expression in Nicotiana benthamiana, an experimental plant host, further promoted Phytophthora infection. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Further ectopic transient overexpression in Nicotiana benthamiana leaf tissue and stable overexpression in Arabidopsis thaliana indicated that PrDGAT3 significantly enhanced the accumulation of the TAG and UFAs. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we demonstrate that 40-nm layered double hydroxide (LDH) nanoparticles are rapidly taken up by intact Nicotiana benthamiana leaf cells and by chloroplasts, following their application via infiltration. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this study, 16S rRNA high-throughput sequencing was applied to study how summer climatic factors and bacterial wildfire disease have affected the composition and assembly of the bacterial community of tobacco (Nicotiana tabacum L.) phyllosphere. Our results indicated that three time series groups (T1, T2 and T3) formed significantly distinct clusters. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The transient expression of TaG6PDH2 in Nicotiana benthamiana leaves and wheat protoplasts revealed that TaG6PDH2 is a chloroplast-targeting protein. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we demonstrate production of V(HH) nanobodies against the SARS-CoV-2 spike proteins in the solanaceous plant Nicotiana benthamiana through transient expression and their subsequent detection verified through western blot. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We examined a collection of live pollen tubes of tobacco (Nicotiana tabacum L.) and hyacinth (Hyacinthus orientalis L.) using a non-invasive semiconductor–electrolyte interface technique in the vicinity of the germination temperature or optimum growth temperature of a pollen grains/tubes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Successful plant reproduction and fruit formation depend on adequate pollen and pistil development, and pollen–pistil interactions. In Nicotiana tabacum, pollen tubes grow through the intercellular spaces of pistil-specialized tissues, stigmatic secretory zone, and stylar transmitting tissue (STT). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We examined their expression pattern in M. polymorpha and subcellular localization using a transient assay with Nicotiana benthamiana. We found that one candidate, MpSGF10B, was expressed in meristems, gemma cups, and male reproductive organs called antheridiophores. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The transient overexpression of the ShWRKY81-GFP (green fluorescent protein) fusion protein in Nicotiana benthamiana cells revealed that ShWRKY81 was localized in the nucleus. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Three potato miRNAs (novel 72, 133, and 140) were predicted to have targets only in the P. infestans genome. miRNAs transient expression and P. infestans inoculation assay showed that miR396, miR166, miR6149-5P, novel133, or novel140 promoted P. infestans colonization, while miR394 inhibited colonization on Nicotiana benthamiana leaves. An artificial miRNA target (amiRNA) degradation experiment demonstrated that miR394 could target both potato gene (PGSC0003DMG400034305) and P. infestans genes. miR396 targets the multicystatin gene (PGSC0003DMG400026899) and miR6149-5p could shear the galactose oxidase F-box protein gene CPR30 (PGSC0003DMG400021641). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we report the engineering of a Form 1A Rubisco from the proteobacterium Halothiobacillus neapolitanus in Escherichia coli and tobacco (Nicotiana tabacum) chloroplasts without any cognate chaperones. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Finally, overexpression of PbbHLH1 and PbbHLH2 in Nicotiana tabacum leaves triggered anthocyanin accumulation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Sixteen of the eighteen candidate effectors cause the suppression of BAX-mediated cell death in Nicotiana benthamiana by using an Agrobacterium-mediated transient expression assay, suggesting their involvement in pathogenicity related to secretion effectors. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Defense function assays showed that JA was up-regulated, but Botrytis cinerea lesion size and Myzus persicae reproduction were suppressed by transient expression of MaCFEM85 and MsWAK16 in the model host plant Nicotiana benthamiana. Collectively, these results provide novel insights into the molecular mechanisms underlying interactions of M. anisopliae with host plants.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Heterologous enzyme co-expression studies of ZmTPS27 in Nicotiana benthamiana resulted in geraniol production while ZmTPS8 yielded α-copaene, δ-cadinene and sesquiterpene alcohols consistent with epi-cubebol, cubebol, copan-3-ol and copaborneol matching the association mapping efforts. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In the present study, we show an efficient protocol using a plant virus vector based on a tobravirus, pepper ringspot virus, that was employed for transient expression of severe acute respiratory syndrome coronavirus 2 partial gene fragments of the spike (named S1-N) and the nucleocapsid (named N) proteins in Nicotiana benthamiana plants. Purified proteins yield of 40–60 µg/g of fresh leaves were obtained. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Cucumber mosaic virus (CMV) is vectored by aphids, including Myzus persicae. Tobacco (Nicotiana tabacum ‘Xanthi’) plants infected with a mutant of the Fny strain of CMV (Fny‐CMVΔ2b, which cannot express the CMV 2b protein) exhibit strong resistance against M. persicae, which is manifested by decreased survival and reproduction of aphids confined on the plants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso