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2301por Hunter, Lydia J. R., Brockington, Samuel F., Murphy, Alex M., Pate, Adrienne E., Gruden, Kristina, MacFarlane, Stuart A., Palukaitis, Peter, Carr, John P.“…RDR1 has been studied in several species including A. thaliana, tobacco (Nicotiana tabacum), N. benthamiana, N. attenuata and tomato (Solanum lycopersicum) but not to our knowledge in potato (S. tuberosum). …”
Publicado 2016
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2302por Zhang, Meijuan, Deng, Xiping, Yin, Lina, Qi, Lingyun, Wang, Xinyue, Wang, Shiwen, Li, Hongbing“…Transgenic tobacco (Nicotiana tabacum) overexpressing rice monogalactosyldiacylglycerol (MGDG) synthase (OsMGD) gene and wild-type tobacco plants were exposed to AlCl(3), and the impact of Al toxicity on root growth, Al accumulation, plasma membrane integrity, lipid peroxidation and membrane lipid composition were investigated. …”
Publicado 2016
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2303“…Recently, in P. hybrida and Nicotiana attenuata, homologs of the Arabidopsis clock gene LATE ELONGATED HYPOCOTYL (LHY) have been shown to have a similar role in the circadian clock in these plants, and to also determine the timing of scent emission. …”
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2304por Chen, Bisi, Niu, Fangfang, Liu, Wu-Zhen, Yang, Bo, Zhang, Jingxiao, Ma, Jieyu, Cheng, Hao, Han, Feng, Jiang, Yuan-Qing“…Furthermore, we identified a previously functionally unknown MYB gene-BnaMYB78, which modulates reactive oxygen species (ROS)-dependent cell death in Nicotiana benthamiana, through regulating the transcription of a few ROS- and defence-related genes. …”
Publicado 2016
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2305“…ObTLP1 encodes a 226 amino acid polypeptide that showed sequence and structural similarities with a sweet-tasting protein thaumatin of Thaumatococcus danielli and also with a stress-responsive protein osmotin of Nicotiana tabacum. The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations. …”
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2306por Stoddard, Thomas J., Clasen, Benjamin M., Baltes, Nicholas J., Demorest, Zachary L., Voytas, Daniel F., Zhang, Feng, Luo, Song“…Here, we tested a new approach for SSN delivery to plant cells, namely transformation of messenger RNA (mRNA) encoding TAL effector nucleases (TALENs). mRNA delivery of a TALEN pair targeting the Nicotiana benthamiana ALS gene resulted in mutation frequencies of approximately 6% in comparison to DNA delivery, which resulted in mutation frequencies of 70.5%. mRNA delivery resulted in three-fold fewer insertions, and 76% were <10bp; in contrast, 88% of insertions generated through DNA delivery were >10bp. …”
Publicado 2016
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2307“…By using transient expression assays in Nicotiana benthamiana, we found that 17 of these effector candidates fully suppressed programmed cell death elicited by a range of cell death-inducing proteins, including BAX, INF1, PsCRN63, PsojNIP, PvRxLR16 and R3a/Avr3a. …”
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2308por Li, Jieqin, Fan, Feifei, Wang, Lihua, Zhan, Qiuwen, Wu, Peijin, Du, Junli, Yang, Xiaocui, Liu, Yanlong“…Transient expression assays in Nicotiana benthamiana leaves demonstrated that the three CCR proteins were localized in the cytoplasm. …”
Publicado 2016
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2309por Merrilees, Mervyn J., Zuo, Ning, Evanko, Stephen P., Day, Anthony J., Wight, Thomas N.“…Recombinant human G1 (rhG1) with an N-terminal 8 amino acid histidine (His) tag, produced in Nicotiana benthamiana, was applied to cultures of dermal fibroblasts, and effects on proliferation and pericellular HA organization determined. rhG1 located to individual strands of cell surface HA which aggregated into structures resembling HA cables. …”
Publicado 2016
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2310por Yanagawa, Yuki, Yoda, Hiroshi, Osaki, Kohei, Amano, Yuta, Aono, Mitsuko, Seo, Shigemi, Kuchitsu, Kazuyuki, Mitsuhara, Ichiro“…Previously, we characterized a MAPK from tobacco, Nicotiana tabacum MPK4 (NtMPK4). Here, we found a highly homologous gene, NtMPK4-like (NtMPK4L), in tobacco as well as other species in Solanaceae and Gramineae. …”
Publicado 2016
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2311por Yu, Lin-Hui, Wu, Jie, Tang, Hui, Yuan, Yang, Wang, Shi-Mei, Wang, Yu-Ping, Zhu, Qi-Sheng, Li, Shi-Gui, Xiang, Cheng-Bin“…In addition, NLP7 improves plant growth and nitrogen use in transgenic tobacco (Nicotiana tabacum). Our results demonstrate that NLP7 significantly improves plant growth under both nitrogen-poor and -rich conditions by coordinately enhancing nitrogen and carbon assimilation and sheds light on crop improvement.…”
Publicado 2016
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2312por Ampah-Korsah, Henry, Anderberg, Hanna I., Engfors, Angelica, Kirscht, Andreas, Norden, Kristina, Kjellstrom, Sven, Kjellbom, Per, Johanson, Urban“…In this article, we present data on two different splice variants (α and β) of NbXIP1;1 from Nicotiana benthamiana. We describe the heterologous expression of NbXIP1;1α and β in the yeast Pichia pastoris, the subcellular localization of the protein in this system and the purification of the NbXIP1;1α protein. …”
Publicado 2016
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2313por Blagborough, A.M., Musiychuk, K., Bi, H., Jones, R.M., Chichester, J.A., Streatfield, S., Sala, K.A., Zakutansky, S.E., Upton, L.M., Sinden, R.E., Brian, I., Biswas, S., Sattabonkot, J., Yusibov, V.“…Using a plant virus-based expression system, we produced recombinant P25 protein of Plasmodium vivax (Pvs25) in Nicotiana benthamiana fused to a modified lichenase carrier protein. …”
Publicado 2016
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2314“…We previously screened several Nicotiana benthamiana (Nb) proteins that interact with the stem-loop 1 (SL1) RNA structure located at the 5′ end of the potato virus X (PVX) genome. …”
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2315por Tsekoa, Tsepo Lebiletsa, Lotter-Stark, Therese, Buthelezi, Sindisiwe, Chakauya, Ereck, Stoychev, Stoyan H., Sabeta, Claude, Shumba, Wonderful, Phahladira, Baby, Hume, Steve, Morton, Josh, Rupprecht, Charles E., Steinkellner, Herta, Pauly, Michael, Zeitlin, Larry, Whaley, Kevin, Chikwamba, Rachel“…Expression levels of up to 490 mg/kg of recombinant mAbs were obtained in Nicotiana benthamiana glycosylation mutants by using a viral based transient expression system. …”
Publicado 2016
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2316“…Expression of Me47 in Nicotiana benthamiana enhanced reproductive performance of green peach aphid (Myzus persicae). …”
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2317por Gao, Ying, Jia, Shuangwei, Wang, Chunlian, Wang, Fujun, Wang, Fajun, Zhao, Kaijun“…Transiently expressed BjMYB1 up-regulated the activity of the BjC-P promoter through its binding to the Wbl-4 element in tobacco (Nicotiana benthamiana) leaves. In B. juncea, BjMYB1 displayed a similar induced expression pattern as that of BjCHI1 upon infection by the fungus Botrytis cinerea. …”
Publicado 2016
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2318por Ray, Soham, Singh, Pankaj K., Gupta, Deepak K., Mahato, Ajay K., Sarkar, Chiranjib, Rathour, Rajeev, Singh, Nagendra K., Sharma, Tilak R.“…Yeast-two-hybrid analysis showed that Mo-01947_9 protein physically interacts with Pi54 protein. Nicotiana benthamiana leaf infiltration assay confirmed induction of hypersensitive response in the presence of Pi54 gene in a heterologous system. …”
Publicado 2016
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2319por Noronha, Henrique, Araújo, Diogo, Conde, Carlos, Martins, Ana P., Soveral, Graça, Chaumont, François, Delrot, Serge, Gerós, Hernâni“…In this work, we cloned a XIP from grapevine, VvXIP1, and agrobacterium-mediated transformation studies in Nicotiana benthamiana revealed that the encoded aquaporin shows a preferential localization at the endoplasmic reticulum membrane. …”
Publicado 2016
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2320por Pasoreck, Elise K., Su, Jin, Silverman, Ian M., Gosai, Sager J., Gregory, Brian D., Yuan, Joshua S., Daniell, Henry“…Therefore, squalene biosynthesis genes FARNESYL DIPHOSPHATE SYNTHASE (FPS) and SQUALENE SYNTHASE (SQS) were engineered via the Nicotiana tabacum chloroplast (C), nuclear (N) or both (CN) genomes to promote squalene biosynthesis. …”
Publicado 2016
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