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2401por López-Gresa, María Pilar, Payá, Celia, Ozáez, Miguel, Rodrigo, Ismael, Conejero, Vicente, Klee, Harry, Bellés, José María, Lisón, Purificación“…HB-mediated stomatal closure was also effective in several plant species belonging to Nicotiana, Arabidopsis, Medicago, Zea and Citrus genus, and both stomatal closure and resistance were induced in HB-treated NahG tomato plants, which are deficient in salicylic acid (SA) accumulation. …”
Publicado 2018
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2402por Conlan, Brendon, Stoll, Thomas, Gorman, Jeffrey J., Saur, Isabel, Rathjen, John P.“…Five AvrPto proximal plant proteins (APPs) were identified and their effect on plant immune function and growth was examined in Nicotiana benthamiana leaves. One protein identified, RIN4, is a central immune component previously shown to interact with AvrPto. …”
Publicado 2018
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2403por Yan, Qiang, Si, Jierui, Cui, Xiaoxia, Peng, Hao, Jing, Maofeng, Chen, Xin, Xing, Han, Dou, Daolong“…Heterologous expression of GmDAD1 in Nicotiana benthamiana enhanced its resistance to Phytophthora parasitica. …”
Publicado 2019
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2404“…Here, the expression of the tobacco (Nicotiana tabacum) hemoglobin gene NtHb1 in Arabidopsis (Arabidopsis thaliana) showed higher cadmium (Cd) tolerance and lower accumulations of Cd, nitric oxide (NO), and reactive oxygen species (ROS) like hydrogen peroxide (H(2)O(2)). …”
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2405por Li, Xixi, Wei, Wenjie, Li, Fangfang, Zhang, Lin, Deng, Xia, Liu, Ying, Yang, Shushen“…Subcellular localization assay indicated that the TaGAPCp1 protein was localized in the plastid of tobacco (Nicotiana tobacum) protoplast. In addition, quantitative real-time PCR (qRT-PCR) unraveled that the expression of TaGAPCp1 (GenBank: MF477938.1) was evidently induced by osmotic stress and abscisic acid (ABA). …”
Publicado 2019
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2406por Lehmann, Marco M, Ghiasi, Shiva, George, Gavin M, Cormier, Marc-André, Gessler, Arthur, Saurer, Matthias, Werner, Roland A“…Here we measured δ(13)C of organic matter (δ(13)C(OM)), concentrations and δ(13)C of potential respiratory substrates, δ(13)C of dark-respired CO(2) (δ(13)C(R)), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum). The strongest δ(13)C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ(13)C(OM), δ(13)C(R), and δ(13)C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle. …”
Publicado 2019
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2407por Arrivault, Stéphanie, Alexandre Moraes, Thiago, Obata, Toshihiro, Medeiros, David B, Fernie, Alisdair R, Boulouis, Alix, Ludwig, Martha, Lunn, John E, Borghi, Gian Luca, Schlereth, Armin, Guenther, Manuela, Stitt, Mark“…We profiled CBC intermediates in a panel of C(4) (Zea mays, Setaria viridis, Flaveria bidentis, and F. trinervia) and C(3) species (Oryza sativa, Triticium aestivum, Arabidopsis thaliana, Nicotiana tabacum, and Manihot esculenta). Principal component analysis revealed differences between C(4) and C(3) species that were driven by many metabolites, including lower ribulose 1,5-bisphosphate in C(4) species. …”
Publicado 2019
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2408por Saberianfar, Reza, Chin-Fatt, Adam, Scott, Andrew, Henry, Kevin A., Topp, Edward, Menassa, Rima“…The antibodies were produced in leaves of Nicotiana benthamiana with production levels ranging between 1 and 3% of total soluble protein. in vivo assembly of all subunits into a hetero-multimeric complex was verified by co-immunoprecipitation. …”
Publicado 2019
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2409“…Here we investigate the presence of an autoregulatory system involving WRI1 through transient expression in Nicotiana benthamiana leaves. Different lengths of the Arabidopsis WRI1 promotor region were coupled to a GUS reporter gene and the activity was measured when combined with constitutive expression of different WRI1 homologs from Arabidopsis thaliana, oat (Avena sativa L.), yellow nutsedge (Cyperus esculentus L.), and potato (Solanum tuberosum L.). …”
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2410por Kirienko, Anna N., Vishnevskaya, Nadezhda A., Kitaeva, Anna B., Shtark, Oksana Yu., Kozyulina, Polina Yu., Thompson, Richard, Dalmais, Marion, Bendahmane, Abdelhafid, Tikhonovich, Igor A., Dolgikh, Elena A.“…PsK1 with replacement in kinase domain and PsSYM10 co-production in Nicotiana benthamiana leaves did not induce a hypersensitive response (HR) because of the impossibility of signal transduction into the cell. …”
Publicado 2019
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2411“…The library was inoculated into the natural host Nicotiana tabacum, allowing competition among all these mutants and selection of those that are potentially viable. …”
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2412por Fang, Anfei, Gao, Han, Zhang, Nan, Zheng, Xinhang, Qiu, Shanshan, Li, Yuejiao, Zhou, Shuang, Cui, Fuhao, Sun, Wenxian“…Transient expression of SCRE2 in Nicotiana benthamiana suppressed necrosis-like defense symptoms triggered by the mammalian BAX and oomycete elicitin INF1 proteins. …”
Publicado 2019
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2413por Rauf, Imran, Javaid, Shaista, Naqvi, Rubab Zahra, Mustafa, Tanveer, Amin, Imran, Mukhtar, Zahid, Jander, Georg, Mansoor, Shahid“…Previously, we reported a double gene construct expressing Hvt and lectin in tobacco (Nicotiana tabacum) plants under phloem specific promoters which confers resistance against hemipteran insects. …”
Publicado 2019
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2414por Pesti, Réka, Kontra, Levente, Paul, Kenny, Vass, Imre, Csorba, Tibor, Havelda, Zoltán, Várallyay, Éva“…In this study, we investigated the gene expression changes induced in CymRSV-, crTMV-, and TCV-infected Nicotiana benthamiana and in PVX- and TMV-U1-infected Solanum lycopersicum plants after the systemic spread of the virus by two different high-throughput methods: microarray hybridization or RNA sequencing. …”
Publicado 2019
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2415por Shi, Xiaoqing, Cordero, Teresa, Garrigues, Sandra, Marcos, Jose F., Daròs, José‐Antonio, Coca, María“…Using this simple viral vector, we have efficiently produced two different AFPs in Nicotiana benthamiana leaves, namely the Aspergillus giganteus AFP and the Penicillium digitatum AfpB. …”
Publicado 2018
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2416por Jackson, Mark A., Yap, Kuok, Poth, Aaron G., Gilding, Edward K., Swedberg, Joakim E., Poon, Simon, Qu, Haiou, Durek, Thomas, Harris, Karen, Anderson, Marilyn A., Craik, David J.“…We show that transient expression in Nicotiana benthamiana allows for rapid peptide production, provided that asparaginyl endopeptidase enzymes with peptide-ligase functionality are co-expressed with the substrate peptide gene. …”
Publicado 2019
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2417por Imamura, Tomohiro, Isozumi, Noriyoshi, Higashimura, Yasuki, Miyazato, Akio, Mizukoshi, Hiroharu, Ohki, Shinya, Mori, Masashi“…Production of amaranthin in a transient Nicotiana benthamiana expression system was evaluated for these candidates and one was identified as having the ability to produce amaranthin. …”
Publicado 2018
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2418por Zhao, Zhibo, Chen, Jiliang, Gao, Xiaoning, Zhang, Di, Zhang, Jinlong, Wen, Jing, Qin, Huqiang, Guo, Ming, Huang, Lili“…A case study of the high‐ and low‐virulence strains in the clade 2 of Psa3 revealed that an hfq variant involved in in vitro growth and virulence, while a conserved locus 930 bp upstream of the hrpR gene in the Type III secretion system (T3SS) cluster was required for full pathogenicity on kiwifruit and elicitation of the hypersensitivity response on non‐host Nicotiana benthamiana. The ‘‐930’ locus is involved in transcriptional regulation of hrpR/S and modulates T3SS function via the hierarchical ‘HrpR/S‐HrpL‐T3SS/effector’ regulatory cascade in Psa. …”
Publicado 2019
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2419por Li, Yuanyuan, Sun, Qian, Zhao, Tianyu, Xiang, Haiying, Zhang, Xiaoyan, Wu, Zhanyu, Zhou, Cuiji, Zhang, Xin, Wang, Ying, Zhang, Yongliang, Wang, Xianbing, Li, Dawei, Yu, Jialin, Dinesh‐Kumar, Savithramma P., Han, Chenggui“…Furthermore, silencing of a Nicotiana benthamiana SKP1 ortholog leads to the destabilization of P0(Br). …”
Publicado 2019
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2420por Maggini, Valentina, Mengoni, Alessio, Gallo, Eugenia Rosaria, Biffi, Sauro, Fani, Renato, Firenzuoli, Fabio, Bogani, Patrizia“…An in vitro model, in which E. purpurea axenic plants as host species and E. angustifolia and Nicotiana tabacum as non-host species inoculated with single endophytes isolated from stem/leaf, root and rhizospheric soil, were used to investigate bacterial colonization. …”
Publicado 2019
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