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  1. 2421
    “…The virus was mechanically transmitted to Nicotiana benthamiana, M. lupulina, M. sativa, Pisum sativum and Vicia faba. …”
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    A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria
  2. 2422
    “…RESULTS: Expression of ZmPGH1 inhibited the HR-inducing activity of both Rp1-D21 and that of another autoactive NLR, RPM1(D505V), in a Nicotiana benthamiana transient expression assay system. …”
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    A maize polygalacturonase functions as a suppressor of programmed cell death in plants
  3. 2423
    “…Both HaVAP1 and HaVAP2 are secreted when expressed in Nicotiana benthamiana leaves, but HaVAP1 is delivered into chloroplasts, whereas HaVAP2 is translocated to the nucleus without signal peptides. …”
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    Two venom allergen‐like proteins, HaVAP1 and HaVAP2, are involved in the parasitism of Heterodera avenae
  4. 2424
    “…Silencing of PpE4 in P. parasitica resulted in significantly reduced virulence on Nicotiana benthamiana. Transient expression of PpE4 in N. benthamiana in turn restored the pathogenicity of the PpE4‐silenced lines. …”
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    An RXLR effector secreted by Phytophthora parasitica is a virulence factor and triggers cell death in various plants
  5. 2425
    “…Amongst these, VmHEP1 and VmHEP2 were found to be up‐regulated during the early infection stage and VmHEP1 was also identified as a cell death inducer through its transient expression in Nicotiana benthamiana. Although the deletion of each single VmHEP gene did not lead to a reduction in virulence, the double‐deletion of VmHEP1 and VmHEP2 notably attenuated V. mali virulence in both apple twigs and leaves. …”
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    Hce2 domain‐containing effectors contribute to the full virulence of Valsa mali in a redundant manner
  6. 2426
    “…Moreover, targeted deletion of the individual LysM effector genes in V. dahliae strain JR2 did not compromise virulence in infections on Arabidopsis, tomato or Nicotiana benthamiana. Interestingly, an additional lineage‐specific LysM effector is encoded in the genome of V. dahliae strain VdLs17, but not in any other V. dahliae strain sequenced to date. …”
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    Verticillium dahliae LysM effectors differentially contribute to virulence on plant hosts
  7. 2427
    “…The recombinant ScFv was generated in Nicotiana benthamiana by transient expression, and was chemically conjugated (ScFv/RVG) to a 29 amino acid peptide, specific for nicotinic acetylcholine receptor (nAchR) binding in the CNS. …”
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    In vitro and in vivo evaluation of a single chain antibody fragment generated in planta with potent rabies neutralisation activity
  8. 2428
    “…Heterologous expression of ZosmaNRT2-GFP together with the high-affinity nitrate transporter accessory protein ZosmaNAR2 (Zosma63g00220.1) in Nicotiana benthamiana leaves displayed four-fold higher fluorescence intensity than single expression of ZosmaNRT2-GFP suggesting the stabilization of NRT2 by NAR2. …”
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    Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.
  9. 2429
    “…Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. …”
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    Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
  10. 2430
    “…One representative compound, BCD03 (3,4-dichloro-benzyloxy carbonimidoyl dicyanide), dramatically reduced pathogenicity of A. citrulli on melon seedlings, and attenuated hypersensitive responses in the non-host Nicotiana tabacum caused by pathogenic bacteria A. citrulli, Xanthomonas oryzae pv. oryzae and Pseudomonas syringae pv. tomato at sub-MIC concentrations. …”
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    Identification of Benzyloxy Carbonimidoyl Dicyanide Derivatives as Novel Type III Secretion System Inhibitors via High-Throughput Screening
  11. 2431
    “…Through transient and stable expression assays in Nicotiana benthamiana and Arabidopsis thaliana, we demonstrate that IYO contains two nuclear localization signals (NLSs), located at the N- and C-terminus of the protein, which mediate the interaction with the NLS-receptor IMPA4 and the import of the protein into the nucleus. …”
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    Identification of Domains and Factors Involved in MINIYO Nuclear Import
  12. 2432
    “…In this study, we performed functional studies on putative effectors in R. solani and revealed that AGLIP1 out of 13 putative effectors induced cell death in Nicotiana benthamiana. AGLIP1 was also demonstrated to trigger cell death in rice protoplasts. …”
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    The Effector AGLIP1 in Rhizoctonia solani AG1 IA Triggers Cell Death in Plants and Promotes Disease Development Through Inhibiting PAMP-Triggered Immunity in Arabidopsis thaliana
  13. 2433
    “…Using in vivo pathway reconstruction assays in heterologous organisms such as Saccharomyces cerevisiae and Nicotiana benthamiana, we have analyzed several cytochrome P450 enzyme genes of the CYP71AX subfamily from Helianthus annuus clustered in close proximity to one another on the sunflower genome. …”
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    Characterization of CYP71AX36 from Sunflower (Helianthus annuus L., Asteraceae)
  14. 2434
    “…(Tubiflorae: Solanaceae), Nicotiana tabacum L. (Tubiflorae: Solanaceae), Raphanus sativus L. …”
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    Effects of Five Host Plant Species on the Life History and Population Growth Parameters of Myzus persicae (Hemiptera: Aphididae)
  15. 2435
    “…Subcellular localization analysis indicated that XsLEA recombinant proteins are differentially distributed in the cytoplasm, membranes and nucleus of Nicotiana benthamiana leaves. Interestingly, a LEA_1 family protein (XsLEA1-8), showing the highest disorder-to-order propensity and protective ability in vitro and in vivo, was also able to enhance salt and drought stress tolerance in Arabidopsis thaliana. …”
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    Structural Plasticity of Intrinsically Disordered LEA Proteins from Xerophyta schlechteri Provides Protection In Vitro and In Vivo
  16. 2436
    “…A transient expression experiment in Nicotiana benthamiana leaves showed that CnTCP2 was able to activate the CnTCP4 promoter. …”
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    The Heterologous Expression of a Chrysanthemum nankingense TCP Transcription Factor Blocks Cell Division in Yeast and Arabidopsis thaliana
  17. 2437
    “…Additionally, the heterologous expression of MaDFR in tobacco (Nicotiana tabacum) resulted in increasing anthocyanin accumulation, leading to a darker flower color, which suggested that MaDFR was involved in color development in flowers. …”
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    Cloning and Functional Characterization of Dihydroflavonol 4-Reductase Gene Involved in Anthocyanidin Biosynthesis of Grape Hyacinth
  18. 2438
    “…Moreover, MG599854 seems to be enough to produce cell death in Nicotiana benthamiana under transient expression conditions. …”
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    DnaJ molecules as potential effectors in Meloidogyne arenaria. An unexplored group of proteins in plant parasitic nematodes
  19. 2439
    “…In this study, we perform the first reporter gene tagging of a CBSV IC, with the insertion of green fluorescent protein (GFP) sequence at two different genome positions. Nicotiana benthamiana infections with the CBSV_GFP ICs revealed active CBSV replication in inoculated leaves at 2–5 days post inoculation (dpi) and systemic leaves at 10–14 dpi. …”
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    Utilization of infectious clones to visualize Cassava brown streak virus replication in planta and gain insights into symptom development
  20. 2440
    “…Here, we present data on the characterization of 46 promoters and 10 terminators in Medicago truncatula, Lotus japonicus, Nicotiana benthamiana and Hordeum vulgare, as well as the effects of codon optimization and intron‐mediated enhancement on the expression of two transgenes in H. vulgare. …”
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    Characterizing standard genetic parts and establishing common principles for engineering legume and cereal roots
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