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3101por Sun, Haiyan, Yang, Ming, Lai, Huafang, Neupane, Biswas, Teh, Audrey Y.-H., Jugler, Collin, Ma, Julian K.-C., Steinkellner, Herta, Bai, Fengwei, Chen, Qiang“…To this end, we selected a ZIKV-specific mAb (ZV54) and generated three ZV54 variants using Chinese hamster ovary cells and wild-type (WT) and glycoengineered ΔXF Nicotiana benthamiana plants as production hosts (ZV54(CHO), ZV54(WT), and ZV54(ΔXF)). …”
Publicado 2023
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3102“…MATERIALS AND METHODS: In this study, two mechanically transmissible begomoviruses, tomato leaf curl New Delhi virus-oriental melon isolate (ToLCNDV-OM) and tomato yellow leaf curl Thailand virus (TYLCTHV), and two nonmechanically transmissible begomoviruses, ToLCNDV-cucumber isolate (ToLCNDV-CB) and tomato leaf curl Taiwan virus (ToLCTV), were used to study the effects of virus-virus interactions on mechanical transmissibility. RESULTS: Nicotiana benthamiana and host plants were coinoculated through mechanical transmission with inoculants derived from plants that were mix-infected or inoculants derived from individually infected plants, and the inoculants were mixed immediately before inoculation. …”
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3103por Oh, Soohyun, Kim, Sejun, Park, Hyo‐Jeong, Kim, Myung‐Shin, Seo, Min‐Ki, Wu, Chih‐Hang, Lee, Hyun‐Ah, Kim, Hyun‐Soon, Kamoun, Sophien, Choi, Doil“…Selected effectors were tested against 436 genome‐wide cloned pepper NLRs, and we identified multiple functional NLRs that recognize P. infestans effectors and confer disease resistance in the Nicotiana benthamiana as a surrogate system. The identified NLRs were homologous to known NLRs derived from wild potatoes that recognize P. infestans effectors such as Avr2, Avrblb1, Avrblb2, and Avrvnt1. …”
Publicado 2023
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3104por Yang, Xiaowen, Gu, Li, Liu, Huixi, Liu, Chensi, Yuan, Jiduan, Qian, Sheng, Wang, Jianming, Yuan, Feiyue, Zhang, Zhongyi, Mu, Jing, Li, Mingjie“…This sequence was cloned from P. heterophylla, and its infectivity was evaluated using the virus-infection model plant Nicotiana benthamiana (N. benthamiana) and host plant P. heterophylla. …”
Publicado 2023
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3105por Falcioni, Renan, dos Santos, Glaucio Leboso Alemparte Abrantes, Crusiol, Luis Guilherme Teixeira, Antunes, Werner Camargos, Chicati, Marcelo Luiz, de Oliveira, Roney Berti, Demattê, José A. M., Nanni, Marcos Rafael“…In this study, UV−VIS−NIR−SWIR reflectance hyperspectral data were collected from Nicotiana tabacum L. plants using a spectroradiometer. …”
Publicado 2023
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3106“…Several studies have approached the expression of cardosin A in Arabidopsis thaliana and Nicotiana tabacum with promising results for the use of these systems to study of cardosin A trafficking. …”
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3107por Contreras, María José, Leal, Karla, Bruna, Pablo, Nuñez-Montero, Kattia, Goméz-Espinoza, Olman, Santos, Andrés, Bravo, León, Valenzuela, Bernardita, Solis, Francisco, Gahona, Giovanni, Cayo, Mayra, Dinamarca, M. Alejandro, Ibacache-Quiroga, Claudia, Zamorano, Pedro, Barrientos, Leticia“…Therefore, in this study, we described and compared the composition of the rhizobacterial community associated with Deschampsia antarctica (Da), Colobanthus quitensis (Cq) from Antarctic territories, and Croton chilensis (Cc), Eulychnia iquiquensis (Ei) and Nicotiana solanifolia (Ns) from coastal Atacama Desert environments by using 16S rRNA amplicon sequencing. …”
Publicado 2023
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3108“…Silencing of Beclin1 in Nicotiana benthamiana plants abolishes the RdRP-mediated degradation of SlHAKAI, indicating the requirement of Beclin1 in this process. …”
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3109por Yuan, Pu, Qian, Wenhao, Jiang, Lihua, Jia, Conghui, Ma, Xiaoxuan, Kang, Zhensheng, Liu, Jie“…Transient expression of PsCAT1 in Nicotiana benthamiana suppressed Bax-induced cell death. …”
Publicado 2021
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3110por Zhao, Siwen, Gong, Pan, Ren, Yanxiang, Liu, Hui, Li, Hao, Li, Fangfang, Zhou, Xueping“…Ectopic expression of C5 using a potato virus X (PVX) vector resulted in severe mosaic symptoms and higher virus accumulation levels followed by a burst of reactive oxygen species (ROS) in Nicotiana benthamiana plants. C5 was able to effectively suppress local and systemic post-transcriptional gene silencing (PTGS) induced by single-stranded GFP but not double-stranded GFP, and reversed the transcriptional gene silencing (TGS) of GFP. …”
Publicado 2022
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3111por Jiao, Tianming, Huang, Yipeng, Wu, Ying-Ling, Jiang, Ting, Li, Tongtong, Liu, Yanzhuo, Liu, Yvchen, Han, Yunyun, Liu, Yajun, Jiang, Xiaolan, Gao, Liping, Xia, Tao“…Three genetic transformation systems (C. sinensis, Nicotiana tabacum, and Arabidopsis thaliana) were used to verify the biological function of CsMYB5s. …”
Publicado 2023
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3112por Si, Xiaolin, Lyu, Shiheng, Hussain, Quaid, Ye, Hongyu, Huang, Chunying, Li, Yan, Huang, Jianqin, Chen, Jianjun, Wang, Ketao“…The catalytic function of these CcSADs was further analyzed by heterologous expression in Saccharomyces cerevisiae, Nicotiana benthamiana, and walnut. Functional analysis demonstrated that all CcSADs had fatty acid desaturase activity to catalyze oleic acid biosynthesis. …”
Publicado 2023
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3113“…We created tobacco (Nicotiana tabacum) plants carrying GFP-Fc1, proIGF-I-Fc1, and Mstn-Fc1 fusion genes, in which fusion with the immunoglobulin G Fc domain improved both protein stability and absorption in the small intestine. …”
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3114Molecular and biological investigating of tea plant necrotic ring blotch virus as a worldwide threat“…Mechanical transmission experiments confirmed the ability of the virus to infect Nicotiana rustica and Chenopodium quinoa seedlings, highlighting the risk of mechanical spread within tea plantations. …”
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3115“…Phylogenetic analysis showed evolutionary relationships within the Solanaceae family, particularly in Capsicum, Nicotiana, and Solanum genera. Physicochemical characteristics of those peptides indicated their similarity to antimicrobial peptides. …”
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3116por Simpson, Jeffrey P., Di Leo, Rosa, Dhanoa, Preetinder K., Allan, Wendy L., Makhmoudova, Amina, Clark, Shawn M., Hoover, Gordon J., Mullen, Robert T., Shelp, Barry J.“…Fluorescence microscopic analysis of tobacco (Nicotiana tabacum L.) suspension cells transiently transformed with GR1 linked to the green fluorescent protein (GFP) revealed that GR1 was localized to the cytosol, whereas GR2-GFP was localized to plastids via targeting information contained within its N-terminal 45 amino acids. …”
Publicado 2008
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3117por Meyers, Ann, Chakauya, Ereck, Shephard, Enid, Tanzer, Fiona L, Maclean, James, Lynch, Alisson, Williamson, Anna-Lise, Rybicki, Edward P“…HIV-1 Pr55Gag is a promising HIV-1 vaccine candidate: we compared the expression of this and a truncated Gag (p17/p24) and the p24 capsid subunit in Nicotiana spp. using transgenic plants and transient expression via Agrobacterium tumefaciens and recombinant tobamovirus vectors. …”
Publicado 2008
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3118“…RESULTS: A retrotransposon vector system was developed for gene delivery in plants based on the Tnt1 element from Nicotiana tabacum. Mini-Tnt1 transfer vectors were constructed that lack coding sequences yet retain the 5' and 3' long terminal repeats (LTRs) and adjacent cis sequences required for reverse transcription. …”
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3119por Landry, Nathalie, Ward, Brian J., Trépanier, Sonia, Montomoli, Emanuele, Dargis, Michèle, Lapini, Giulia, Vézina, Louis-P.“…Using a novel manufacturing platform based on transient expression of influenza surface glycoproteins in Nicotiana benthamiana, we have recently demonstrated that a candidate Virus-Like Particle (VLP) vaccine can be generated within 3 weeks of release of sequence information. …”
Publicado 2010
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3120“…Light response (at 300 ppm CO(2) and 10–50 ppm O(2) in N(2)) and CO(2) response curves [at absorbed photon fluence rate (PAD) of 550 μmol m(−2) s(−1)] of O(2) evolution and CO(2) uptake were measured in tobacco (Nicotiana tabacum L.) leaves grown on either NO(3)(−) or NH(4)(+) as N source and in potato (Solanum tuberosum L.), sorghum (Sorghum bicolor L. …”
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