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3201“…METHODS: Sorghum plants were rub-inoculated with sap prepared by grinding 2 g of infected Nicotiana benthamiana leaf in 1 ml 10 mM potassium phosphate buffer (pH 6.8) and 100 mg of carborundum abrasive. …”
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3202por Pospisilova, Sarka, Kos, Jiri, Michnova, Hana, Kapustikova, Iva, Strharsky, Tomas, Oravec, Michal, Moricz, Agnes M., Bakonyi, Jozsef, Kauerova, Tereza, Kollar, Peter, Cizek, Alois, Jampilek, Josef“…Compounds with fungicide potency did not show any in vivo toxicity against Nicotiana tabacum var. Samsun. The structure–activity relationships are discussed.…”
Publicado 2018
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3203por Plunkett, Blue J., Espley, Richard V., Dare, Andrew P., Warren, Ben A. W., Grierson, Ella R. P., Cordiner, Sarah, Turner, Janice L., Allan, Andrew C., Albert, Nick W., Davies, Kevin M., Schwinn, Kathy E.“…In transient assays, MYBA complemented an anthocyanin MYB mutant of Antirrhinum majus and, together with a heterologous bHLH anthocyanin regulator, activated anthocyanin production in Nicotiana benthamiana. Furthermore anthocyanin accumulation and anthocyanin structural gene expression (assayed by qPCR and RNA-seq analyses) correlated with MYBA expression, and MYBA was able to transactivate the DFR promoter from blueberry and other species. …”
Publicado 2018
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3204por Yang, Jian, Zhang, Tian-Ye, Liao, Qian-Sheng, He, Long, Li, Juang, Zhang, Heng-Mu, Chen, Xuan, Li, Jing, Yang, Jin, Li, Jin-Bang, Chen, Jian-Ping“…Chinese wheat mosaic virus (CWMV) has a bipartite, single-strand positive RNA genome, and can infect both wheat and Nicotiana benthamiana, and the optimal temperature for systemic infection in plants is 17°C. …”
Publicado 2018
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3205por Li, Zhijian T., Janisiewicz, Wojciech J., Liu, Zongrang, Callahan, Ann M., Evans, Breyn E., Jurick, Wayne M., Dardick, Chris“…When cultured on Murashige and Skoog (MS) medium under in vitro conditions, tobacco seedlings (Nicotiana tabacum) exposed to TC09 cultures for 20 days increased stem height and whole plant biomass up to 25- and 15-fold, respectively, over controls without exposure. …”
Publicado 2019
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3206por Wang, Zheng, Bao, Ling-Li, Zhao, Feng-Yun, Tang, Min-Qiang, Chen, Ting, Li, Yaoming, Wang, Bing-Xu, Fu, Benzhong, Fang, Hedi, Li, Guan-Ying, Cao, Jun, Ding, Li-Na, Zhu, Ke-Ming, Liu, Sheng-Yi, Tan, Xiao-Li“…Moreover, overexpression (OE) of BnaMPK3 in B. napus and Nicotiana benthamiana results in significantly enhanced resistance to S. sclerotiorum, whereas resistance is diminished in RNAi transgenic plants. …”
Publicado 2019
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3207por Zhao, Lihua, Hu, Zhonghui, Li, Shunlin, Zhou, Xueping, Li, Jing, Su, Xiaoxia, Zhang, Lizhen, Zhang, Zhongkai, Dong, Jiahong“…The AHK-induced resistance to TSWV in Nicotiana benthamiana could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. …”
Publicado 2019
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3208por Pütter, Katharina M., van Deenen, Nicole, Müller, Boje, Fuchs, Lea, Vorwerk, Kirsten, Unland, Kristina, Bröker, Jan Niklas, Scherer, Emely, Huber, Claudia, Eisenreich, Wolfgang, Prüfer, Dirk, Schulze Gronover, Christian“…Furthermore, we isolated six different oxidosqualene cyclases (OSCs) and two P450 enzymes, and we functionally characterized TkOSC1 and CYP716A263 in Nicotiana benthamiana and Saccharomyces cerevisiae in detail. …”
Publicado 2019
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3209por Zhao, Xian-Yan, Qi, Chen-Hui, Jiang, Han, You, Chun-Xiang, Guan, Qing-Mei, Ma, Feng-Wang, Li, Yuan-Yuan, Hao, Yu-Jin“…Ectopic expression of MdWRKY31 in Arabidopsis and Nicotiana benthamiana enhanced plant sensitivity to ABA. …”
Publicado 2019
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3210“…Functional analysis by transient expression in Nicotiana benthamiana leaves and genetic complementary assay using yeast mutant BY4389 strain unable to synthesize unsaturated fatty acids demonstrated that GhA-SAD6 and GhD-SAD8 have strong substrate specificity for 16:0-ACP. …”
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3211“…RESULTS: ONAC066 was localized in nuclei of cells when transiently expressed in Nicotiana benthamiana and is a transcription activator with the binding ability to NAC recognition sequence (NACRS) and AtJUB1 binding site (JBS). …”
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3212“…All eight chimeras were transiently expressed in Nicotiana benthamiana via Agrobacterium tumefaciens-mediated DNA transfer. …”
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3213por Li, Ke, Wang, Juan, Liu, Chuanliang, Li, Changsheng, Qiu, Jingjing, Zhao, Chuanzhi, Xia, Han, Ma, Changle, Wang, Xingjun, Li, Pengcheng“…Our previous study stated that Arabidopsis thaliana LEC2 (AtLEC2) driven by glucocorticoid receptor-dexamethasone (GR-DEX) inducible system (AtLEC2-GR) triggers embryogenic callus formation in tobacco (Nicotiana tabacum). RESULTS: In this study, the adenosine phosphate isopentenyltransferase genes AtIPT3, AtIPT7 and the tRNA isopentenyltransferase gene AtIPT9 were overexpressed in the AtLEC2-GR transgenic background. …”
Publicado 2019
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3214por Wang, Jun, Zeng, Xuan, Tian, Dongsheng, Yang, Xiaobei, Wang, Lanlan, Yin, Zhongchao“…In this study, the synthetic genes of CpBs4C‐R and two other Bs4C‐like genes, the susceptible allele in the genotype PI585270 of C. pubescens (CpBs4C‐S) and the CaBs4C‐R homologue gene in the cultivar ‘CM334’ of Capsicum annum (CaBs4C), were characterized in tobacco (Nicotiana benthamiana) and rice (Oryza sativa). The Bs4C genes induced cell death in N. benthamiana. …”
Publicado 2018
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3215por Kovalskaya, Natalia Y., Herndon, Eleanor E., Foster-Frey, Juli A., Donovan, David M., Hammond, Rosemarie W.“…The TF gene was expressed in Nicotiana benthamiana plants using the non-replicating Cowpea mosaic virus (CPMV)-based vector pEAQ-HT and the replicating Alternanthera mosaic virus (AltMV)-based pGD5TGB1(L88)23-MCS-CP3 vector, and in Escherichia coli using pET expression vectors pET26b+ and pET28a+. …”
Publicado 2019
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3216“…Here we compare the heterologous expression systems used to produce, purify, and study the enzyme activities of PCW GTs, with an emphasis on the eukaryotic systems Nicotiana benthamiana, Pichia pastoris, and human embryonic kidney (HEK293) cells. …”
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3217por Vieira, Jorge, Rocha, Sara, Vázquez, Noé, López-Fernández, Hugo, Fdez-Riverola, Florentino, Reboiro-Jato, Miguel, Vieira, Cristina P.“…Here we first identify putative SLF genes from nine Solanum and 10 Nicotiana genomes to determine how many gene lineages are present in the three genera, and the rate of origin of new SLF gene lineages. …”
Publicado 2019
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3218“…The light-weight microscope allows to image label-free micro-spheres of 6.5 μm diameter, human red-blood-cells of about 8 μm diameter as well as fast-growing plant Nicotiana-tabacum-BY-2 suspension cells with 50 μm sizes. …”
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3219por Liu, Yuhui, Lin-Wang, Kui, Espley, Richard V, Wang, Li, Li, Yuanming, Liu, Zhen, Zhou, Ping, Zeng, Lihui, Zhang, Xiaojing, Zhang, Junlian, Allan, Andrew C“…In transient assays, StMYB44 represses anthocyanin accumulation in leaves of Nicotiana tabacum and N. benthamiana by directly suppressing the activity of the dihydroflavonol reductase (DFR) promoter. …”
Publicado 2019
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3220por Wang, Aijun, Pan, Linxiu, Niu, Xianyu, Shu, Xinyue, Yi, Xiaoqun, Yamamoto, Naoki, Li, Shuangcheng, Deng, Qiming, Zhu, Jun, Liang, Yueyang, Wang, Lingxia, Li, Ping, Zheng, Aiping“…Through transient expression, we demonstrated that two putative effectors could induce necrosis phenotypes in Nicotiana benthamiana. These two encoded genes were up-regulated during early infection, and the encoded proteins were confirmed to be secreted using a yeast secretion system. …”
Publicado 2019
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