“…The FILP system enabled a rapid synthetic promoter screen: starting from 2000 synthetic promoters transfected into protoplasts to FILP‐imaged agroinfiltrated Nicotiana benthamiana plants in a matter of weeks, which was useful to characterize a water stress‐inducible synthetic promoter. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Biochemical analysis using a Nicotiana benthamiana transient expression system revealed weak association between MpCIK and MpCLV1, as well as MpCIK and MpTDR. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…After mutagenizing the Fc, we screened for accumulation levels after transient transformation in Nicotiana benthamiana leaves. We identified and characterized five supercharging and one disulfide mutant, termed ‘(5 + 1)Fc’, that improve accumulation in comparison to the native Fc. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Constitutively active StMPK7 (CA‐StMPK7; StMPK7(D198G, E202A)) was found to promote immunity to Phytophthora pathogens and to trigger host cell death when overexpressed in Nicotiana benthamiana leaves. Cell death triggered by CA‐StMPK7 is SGT1/RAR1‐dependent. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Transient ZmMIEL1 expression in Nicotiana benthamiana reduced HR induced by Rp1‐D21, while suppression of ZmMIEL1 expression in maize carrying Rp1‐D21 increased HR. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We developed a set of in-house expression vectors for transient transformation of Nicotiana benthamiana leaves that target a V(H)H-Fc to the thylakoid lumen via either secretory (Sec) or twin-arginine translocation (Tat) import pathways. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…(Meliaceae), Copaifera paupera (Herzog) Dwyer (Fabaceae), Musa × paradisiaca L. (Musaceae), and Nicotiana tabacum L. (Solanaceae), since they are the most frequently cited in articles and by traditional communities.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Transient overexpression of AaMYB308like suggested overexpression of AaMYB308like elevated transcription level of NtCHI in Nicotiana tabacum leaves. Integration of all these results imply that AaMYB308like might be served as a light-responsive transcription factor to regulate anthocyanin biosynthesis in A. arguta. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These 15 synthetic promoters were screened by transient expression assays in poplar leaf mesophyll protoplasts and agroinfiltrated Nicotiana benthamiana leaves under osmotic stress conditions. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we developed a quantitative in vitro cleavage assay that combines recombinant AtIRE1a protein that is expressed in Nicotiana benthamiana and total RNA isolated from Arabidopsis leaves. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Transient expression of salivary proteins in Nicotiana benthamiana leaves identified that RpSP10.3, RpSP13.4, RpSP13.8, RpSP17.8, and RpSP10.2 were capable of inducing cell death, reactive oxygen species (ROS) burst, and hormone signal changes, indicating the potential roles of these proteins in eliciting plant defenses. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…While cytotoxic activity could be partially suppressed by known effectors, they failed to impede necrosis in Nicotiana benthamiana. Thus, unlike typical effectors, cytotoxicity of VdXyn4 plays a crucial intracellular role at the late stages of V. dahliae infection and colonization, especially following pathogen entry into the xylem; this cytotoxic activity is likely conserved in the corresponding enzyme families in plant vascular pathogens.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two-hybrid analysis showed the ability of SlMADS5 to activate transcription of the target gene and interact with TAGL1. Transgenic plants Nicotiana tabacum L. with constitutive overexpression of SlMADS5 cDNA f lowered 2.2 times later than the control; plants formed thickened leaves, 2.5–3.0 times thicker stems, 1.5–2.7 times shortened internodes, and 1.9 times fewer f lowers and capsules than non-transgenic plants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…A recombinant HEV-3 ORF2 110-610_6his capsid protein, transiently expressed by pEff vector in Nicotiana benthamiana plants was used to develop an in-house HEV EIA. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Many tobacco (Nicotiana tabacum) cultivars are salt-tolerant and thus are potential model plants to study the mechanisms of salt stress tolerance. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…MbA biosynthesis has been reconstructed in Nicotiana benthamiana using montbretia genes for the assembly of MbA from its various different building blocks. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we transiently expressed an RBD variant in wild-type and glycoengineered Nicotiana benthamiana leaves and characterized the impact of different plant-specific N-glycans on RBD reactivity in serological assays. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Similar to other hordeiviruses, LigMV formed rod-shape virions (visualized by electron microscopy), was transmitted through seeds and could also be mechanically transmitted to herbaceous hosts Chenopodium quinoa and Nicotiana benthamiana. Blot hybridization analysis identified genomic and subgenomic RNAs, sharing the 3′-CR and likely serving as monocistronic mRNAs for seven evolutionarily-conserved viral proteins including two subunits of viral RNA-dependent RNA polymerase, coat protein, triple gene block proteins mediating viral movement and cysteine-rich suppressor of RNA silencing. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The results showed that MP was the virulence determinant for ToBRFV to infect Tm‐2(2) transgenic Nicotiana benthamiana plants and Tm‐2(2) ‐carrying tomato plants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso