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3441por Xiang, Gaoqing, Yin, Xiao, Niu, Weili, Chen, Tingting, Liu, Ruiqi, Shang, Boxing, Fu, Qingqing, Liu, Guotian, Ma, Hui, Xu, Yan“…This was in accordance with the result that most PvCRN proteins, except PvCRN11, failed to induce necrosis in Nicotiana benthamiana. Pattern-triggered immunity (PTI) induced by INF1 was hampered by several PvCRN proteins. …”
Publicado 2021
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3442“…ABSTRACT: Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed. Nicotiana benthamiana is widely used for agroinfiltration and the characterisation of broad-spectrum effectors. …”
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3443“…Furthermore, silencing of BcPmk1 in Botrytis cinerea with conserved CcPmk1 and BcPmk1 fragments could significantly compromise fungal virulence using the virus‐induced gene silencing system in Nicotiana benthamiana. These results suggest that CcPmk1 functions as a regulator of pathogenicity and can potentially be designed as a target for broad‐spectrum disease control, but unintended effects on nonpathogenic fungi need to be avoided.…”
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3444por Zeng, Danqi, Teixeira da Silva, Jaime A., Zhang, Mingze, Yu, Zhenming, Si, Can, Zhao, Conghui, Dai, Guangyi, He, Chunmei, Duan, Juan“…The transcriptional activation of DoAP2-1, DoAP2-2, DoAP2-6 and DoAP2-11 proteins, which were localized in the nucleus of Arabidopsis thaliana mesophyll protoplasts, was further analyzed by a dual-luciferase reporter gene system in Nicotiana benthamiana leaves. Our data showed that pBD-DoAP2-1, pBD-DoAP2-2, pBD-DoAP2-6 and pBD-DoAP2-11 significantly repressed the expression of the LUC reporter compared with the negative control (pBD), suggesting that these DoAP2 proteins may act as transcriptional repressors in the nucleus of plant cells. …”
Publicado 2021
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3445por Tuo, Decai, Zhou, Peng, Yan, Pu, Cui, Hongguang, Liu, Yang, Wang, He, Yang, Xiukun, Liao, Wenbin, Sun, Di, Li, Xiaoying, Shen, Wentao“…The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. …”
Publicado 2021
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3446por Mou, Shaoliang, Meng, Qianqian, Gao, Feng, Zhang, Tingting, He, Weihong, Guan, Deyi, He, Shuilin“…Overexpression of CaCRK5 increased resistance against R. solanacearum in Nicotiana benthamiana. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with quantitative real-time PCR analysis revealed that a homeodomain zipper I protein CaHDZ27 can active the expression of CaCRK5 through directly binding to its promoter. …”
Publicado 2021
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3447por Luo, Yanjie, Na, Ren, Nowak, Julia S., Qiu, Yang, Lu, Qing Shi, Yang, Chunyan, Marsolais, Frédéric, Tian, Lining“…Genome editing of endogenous phytoene desaturase (PDS) loci and exogenous 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) sequence in Nicotiana. benthamiana (N. benthamiana) through CCAC was confirmed using Sanger sequencing. …”
Publicado 2021
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3448“…RESULTS: One hundred fifty-nine GDSL esterase/lipase genes from cultivated tobacco (Nicotiana tabacum) were identified, and the dynamic changes in the expression levels of 93 of these genes in response to topping, as assessed using transcriptome data of topping-induced axillary shoots, were analysed. …”
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3449por Kayani, Sadaf-Ilyas, Shen, Qian, Rahman, Saeed-ur, Fu, Xueqing, Li, Yongpeng, Wang, Chen, Hassani, Danial, Tang, Kexuan“…A transient dual-luciferase assay in Nicotiana bethamiana with the AaYABBY5 effector showed a significant increase in the activity of the downstream LUC gene, with reporters AaPAL, AaCHS, AaCHI, and AaUFGT. …”
Publicado 2021
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3450“…Transient expression of HDT in Nicotiana benthamiana resulted in the production of caffeoyl-L-Tyr, but not clovamide. …”
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3451por Bortolami, Alessio, Donini, Marcello, Marusic, Carla, Lico, Chiara, Drissi Touzani, Charifa, Gobbo, Federica, Mazzacan, Elisa, Fortin, Andrea, Panzarin, Valentina Maria, Bonfante, Francesco, Baschieri, Selene, Terregino, Calogero“…In this study, an engineered version of the VP3 protein of IBDV (His-VP3) was produced in plants, successfully purified from Nicotiana benthamiana leaves, and used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-VP3 antibodies. …”
Publicado 2021
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3452por Xu, Liang, Song, Jia-Qian, Wang, Yue-Lin, Liu, Xiao-Han, Li, Xue-Li, Zhang, Bo, Li, Ai-Jie, Ye, Xie-Feng, Wang, Jing, Wang, Peng“…BACKGROUND AND OBJECTIVE: Salt stress is one of the most important abiotic stresses affecting the yield and quality of tobacco (Nicotiana tabacum). Thymol (a natural medicine) has been widely used in medical research because of its antibacterial and anti-inflammatory activities. …”
Publicado 2022
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3453“…Regular expression search using the cleavage site consensus sequence [EQN]xVxH[QE]/[SGTA] for the plum pox virus (PPV) protease identified 90 to 94 putative cleavage events in the proteomes of Prunus persica (a crop severely affected by PPV), Arabidopsis thaliana, and Nicotiana benthamiana (two experimental hosts). In vitro processing assays confirmed cleavage of six A. thaliana and five P. persica proteins by the PPV protease. …”
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3454por Yang, Fengxi, Lu, Chuqiao, Wei, Yonglu, Wu, Jieqiu, Ren, Rui, Gao, Jie, Ahmad, Sagheer, Jin, Jianpeng, Xv, Yechun, Liang, Gang, Zhu, Genfa“…Moreover, overexpression of Ce-miR396 in Nicotiana tabacum resulted in curved pistils and reduced fertility, implying that the conserved role of Ce-miR396 in floral development. …”
Publicado 2022
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3455por Petkova, Mariana, Petrova, Slaveya, Spasova-Apostolova, Velichka, Naydenov, Mladen“…To establish colonization in the various tissues of tested tobacco (Nicotiana tabaccum L.) plants, samples were taken on the seventh, fourteenth, and twenty-first days after treatment (DAT), and explants were inoculated on yeast malt agar (YMA). …”
Publicado 2022
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3456por Strini, Edward J., Bertolino, Lígia T., San Martin, Juca A. B., Souza, Hebréia A. O., Pessotti, Francine, Pinoti, Vitor F., Ferreira, Pedro B., De Paoli, Henrique C., Lubini, Greice, Del-Bem, Luiz-Eduardo, Quiapim, Andréa C., Mondin, Mateus, Araujo, Ana Paula U., Eloy, Nubia B., Barberis, Matteo, Goldman, Maria Helena S.“…Recently, we demonstrated that SCI1 starts to be expressed at the specification of the Nicotiana tabacum floral meristem and is expressed at all floral meristematic cells. …”
Publicado 2022
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3457por Li, Mingzhuo, He, Xianzhi, La Hovary, Christophe, Zhu, Yue, Dong, Yilun, Liu, Shibiao, Xing, Hucheng, Liu, Yajun, Jie, Yucheng, Ma, Dongming, Yuzuak, Seyit, Xie, De-Yu“…METHODS: G-box and MYB recognition elements (MREs) were identified in four Nicotiana tabacum JAZs (NtJAZs) promoters. MRE-like and G-box like elements were identified in one nicotine pathway gene promoter. …”
Publicado 2021
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3458“…Bamboo mosaic virus (BaMV) causes chlorotic mosaic symptoms in both Brachypodium distachyon and Nicotiana benthamiana. The BaMV △CPN35 mutant with an N-terminal deletion of its coat protein gene exhibits asymptomatic infection independently of virus titer. …”
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3459por Agati, Giovanni, Brunetti, Cecilia, Tuccio, Lorenza, Degano, Ilaria, Tegli, Stefania“…In this study, we developed and applied a new spectroscopic fluorescence method for the in vivo detection of the early events in the interaction between tobacco (Nicotiana tabacum L.) plants and pathogenic bacteria. …”
Publicado 2022
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3460por Li, Minhui, Xie, Lifei, Wang, Meng, Lin, Yilian, Zhong, Jiaqi, Zhang, Yong, Zeng, Jing, Kong, Guanghui, Xi, Pinggen, Li, Huaping, Ma, Li-Jun, Jiang, Zide“…Transient expression of FOIG_15013 in Nicotiana benthamiana leaves activates the host defense responses. …”
Publicado 2022
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