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701por Hamorsky, Krystal Teasley, Kouokam, J. Calvin, Jurkiewicz, Jessica M., Nelson, Bailey, Moore, Lauren J., Husk, Adam S., Kajiura, Hiroyuki, Fujiyama, Kazuhito, Matoba, Nobuyuki“…Previously, we have shown that cholera toxin B subunit (CTB), an oral cholera vaccine antigen, is N-glycosylated upon expression in transgenic Nicotiana benthamiana. Here, we found that overexpression of aglycosylated CTB by agroinfiltration of a tobamoviral vector causes massive tissue necrosis and poor accumulation unless retained in the endoplasmic reticulum (ER). …”
Publicado 2015
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702por Wylie, Stephen J., Zhang, Chao, Long, Vicki, Roossinck, Marilyn J., Koh, Shu Hui, Jones, Michael G. K., Iqbal, Sadia, Li, Hua“…Nicotiana benthamiana is a model plant utilised internationally in plant virology because of its apparent hyper-susceptibility to virus infection. …”
Publicado 2015
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703por Grimberg, Åsa, Carlsson, Anders S., Marttila, Salla, Bhalerao, Rishikesh, Hofvander, Per“…Here, the effect on global gene expression as well as on physiological changes in leaves transiently expressing five homologs of the transcription factor WRINKLED1 (WRI1) originating from diverse species and tissues; Arabidopsis thaliana and potato (Solanum tuberosum) seed embryo, poplar (Populus trichocarpa) stem cambium, oat (Avena sativa) grain endosperm, and nutsedge (Cyperus esculentus) tuber parenchyma, were studied by agroinfiltration in Nicotiana benthamiana. RESULTS: All WRI1 homologs induced oil accumulation when expressed in leaf tissue. …”
Publicado 2015
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704“…In contrast, GhWRKY27a overexpression in Nicotiana benthamiana markedly reduced plant tolerance to drought stress, as determined through physiological analyses of leaf water loss, survival rates, and the stomatal aperture. …”
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705“…We expressed the complete set of six cellulases and an additional β-glucosidase expressed from Thermobifida fusca in the bacterium Escherichia coli and in tobacco plants (Nicotiana tabacum). This was done to determine whether functional enzyme expression was feasible in these organisms. …”
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706por Gnanasekaran, Thiyagarajan, Vavitsas, Konstantinos, Andersen-Ranberg, Johan, Nielsen, Agnieszka Zygadlo, Olsen, Carl Erik, Hamberger, Björn, Jensen, Poul Erik“…RESULTS: We transiently expressed the isopimaric acid pathway in Nicotiana benthamiana leaves and enhanced its productivity by the expression of two rate-limiting steps in the pathway (providing the general precursor of diterpenes). …”
Publicado 2015
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707por Li, Zhenhua, Zhang, Jie, Liu, Yiling, Zhao, Jiehong, Fu, Junjie, Ren, Xueliang, Wang, Guoying, Wang, JianhuaEnlace del recurso
Publicado 2016
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708por Petre, Benjamin, Saunders, Diane G. O., Sklenar, Jan, Lorrain, Cécile, Krasileva, Ksenia V., Win, Joe, Duplessis, Sébastien, Kamoun, Sophien“…We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. …”
Publicado 2016
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709por Gnanasekaran, Thiyagarajan, Karcher, Daniel, Nielsen, Agnieszka Zygadlo, Martens, Helle Juel, Ruf, Stephanie, Kroop, Xenia, Olsen, Carl Erik, Motawie, Mohammed Saddik, Pribil, Mathias, Møller, Birger Lindberg, Bock, Ralph, Jensen, Poul Erik“…For this purpose, we stably engineered the dhurrin pathway from Sorghum bicolor into the chloroplasts of Nicotiana tabacum (tobacco). Dhurrin is a cyanogenic glucoside and its synthesis from the amino acid tyrosine is catalysed by two membrane-bound cytochrome P450 enzymes (CYP79A1 and CYP71E1) and a soluble glucosyltransferase (UGT85B1), and is dependent on electron transfer from a P450 oxidoreductase. …”
Publicado 2016
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710por Chu, Xiaoqian, Wang, Chen, Chen, Xiaobo, Lu, Wenjing, Li, Han, Wang, Xiuling, Hao, Lili, Guo, XingqiEnlace del recurso
Publicado 2016
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711“…In this work, we report that virus-induced gene silencing of transcripts encoding a putative RPC5-like subunit of the RNA Polymerase III in a model species Nicotiana benthamiana had pleiotropic effects, including but not limited to severe dwarfing appearance, chlorosis, nearly complete reduction of internodes and abnormal leaf shape. …”
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712por Zhang, Chengsheng, Feng, Chao, Wang, Jing, Kong, Fanyu, Sun, Wenxiu, Wang, Fenglong“…Further, the expression profile of NtPGIP was examined in tobacco leaves following stimulation with the oomycete Phytophthora nicotianae and other stressors, including salicylic acid (SA), abscisic acid (ABA), salt, and cold treatment. …”
Publicado 2016
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713por Liu, Jing, Peng, Huan, Cui, Jiangkuan, Huang, Wenkun, Kong, Lingan, Clarke, Jihong Liu, Jian, Heng, Wang, Guo Liang, Peng, Deliang“…To understand the function of nematode effectors in parasitic plants, we cloned predicted effectors genes from Heterodera avenae and transiently expressed them in Nicotiana benthamiana. Infiltration assays showed that HaEXPB2, a predicted expansin-like protein, caused cell death in N. benthamiana. …”
Publicado 2016
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714por Adlung, Norman, Prochaska, Heike, Thieme, Sabine, Banik, Anne, Blüher, Doreen, John, Peter, Nagel, Oliver, Schulze, Sebastian, Gantner, Johannes, Delker, Carolin, Stuttmann, Johannes, Bonas, Ulla“…Interestingly, the effector XopQ was recognized by several Nicotiana spp. lines, and Xcv infection assays revealed that XopQ is a host range determinant in many Nicotiana species. …”
Publicado 2016
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715por Yin, Guoying, Wang, Wenjing, Niu, Haixia, Ding, Yongqiang, Zhang, Dingyu, Zhang, Jie, Liu, Guanshan, Wang, Sangen, Zhang, Hongbo“…Nicotine is a secondary metabolite that is important to the defense system and commercial quality of tobacco (Nicotiana tabacum L.). Jasmonate and its derivatives (JAs) are phytohormone regulators of nicotine formation; however, the underlying molecular mechanism of this process remains largely unclear. …”
Publicado 2017
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716por Philips, Joshua G., Naim, Fatima, Lorenc, Michał T., Dudley, Kevin J., Hellens, Roger P., Waterhouse, Peter M.“…Nicotiana benthamiana is employed around the world for many types of research and one transgenic line has been used more extensively than any other. …”
Publicado 2017
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718por Lee, Jin Won, Heo, Woon, Lee, Jinu, Jin, Narae, Yoon, Sei Mee, Park, Ki Youl, Kim, Eun Yu, Kim, Woo Taek, Kim, Joo Young“…The preliminary efficacy of ZMapp, a cocktail of antibodies produced in N. benthamiana (Nicotiana benthamiana L.), suggested plants may serve as a platform for antibody production. …”
Publicado 2018
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719“…However, it is unclear whether PDRs are involved in Nicotiana attenuata resistance to the necrotic fungal pathogen Alternaria alternata. …”
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720por Zhang, Peng, Li, Xin, Yuan, Xiao-Long, Du, Yong-Mei, Wang, Bin-Gui, Zhang, Zhong-Feng“…An endophytic fungus Arthrinium arundinis TE-3 was isolated and purified from the fresh leaves of cultivated tobacco (Nicotiana tabacum L.). Chemical investigation on this fungal strain afforded three new prenylated diphenyl ethers (1–3) as well as three known analogues (4–6). …”
Publicado 2018
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