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781“…Treatments with salt, drought, and H(2)O(2) induced the expression of GhMKK1 and increased the activity of GhMKK1, while overexpression of GhMKK1 in Nicotiana benthamiana enhanced its tolerance to salt and drought stresses as determined by many physiological data. …”
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784“…An overexpression construct was then designed and transferred into Nicotiana benthamiana, and PCR and Southern blot showed that the NbZFP1 gene was successfully integrated into the Nicotiana benthamiana genome. …”
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785por Khan, Fariha, Daniëls, Mark A., Folkers, Gert E., Boelens, Rolf, Saqlan Naqvi, S. M., van Ingen, Hugo“…Here, we studied the structural basis of nucleic acid binding of full-length Nicotiana tabacum GR-RBP1. NMR studies of NtGR-RBP1 show that the glycine-rich domain, while intrinsically disordered, is responsible for mediating self-association by transient interactions with its RRM domain (NtRRM). …”
Publicado 2014
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786por Shitan, Nobukazu, Minami, Shota, Morita, Masahiko, Hayashida, Minaho, Ito, Shingo, Takanashi, Kojiro, Omote, Hiroshi, Moriyama, Yoshinori, Sugiyama, Akifumi, Goossens, Alain, Moriyasu, Masataka, Yazaki, Kazufumi“…Following its biosynthesis in root tissues, nicotine, the major alkaloid of Nicotiana species, is translocated via xylem transport toward the accumulation sites, leaf vacuoles. …”
Publicado 2014
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788“…In this context, we tested the fusion of hydrophobin I to the hemagglutinin ectodomain of the influenza A (H1N1)pdm09 virus controlled by the hybrid En(2)PMA4 transcriptional promoter to rapidly produce high levels of recombinant antigen by transient expression in agro-infiltrated Nicotiana benthamiana leaves. The fusion increased the expression level by a factor of ∼2.5 compared to the unfused protein allowing a high accumulation level of 8.6% of the total soluble proteins. …”
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789por Groten, Karin, Pahari, Nabin T., Xu, Shuqing, Miloradovic van Doorn, Maja, Baldwin, Ian T.“…From 92,434 SuperSAGE Tag sequences, 32,808 (35%) matched with our in-house Nicotiana attenuata transcriptome database and 3,698 (4%) matched to Rhizophagus genes. …”
Publicado 2015
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790por Mhlongo, Msizi I., Piater, Lizelle A., Madala, Ntakadzeni E., Steenkamp, Paul A., Dubery, Ian A.“…Here we applied a metabolomic approach to study and compare the responses induced in Nicotiana tabacum cells by microbe-associated molecular pattern (MAMP) molecules, namely lipopolysaccharides (LPS), chitosan (CHT) and flagellin-22 (FLG22). …”
Publicado 2016
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791“…However, the regulatory mechanism of the FIE gene in postembryonic shoot development is not well understood. Silencing of Nicotiana benthamiana homologues of the FIE gene, NbFIE1 and NbFIE2, resulted in the enhanced outgrowth of axillary buds and the impairment of secondary xylem differentiation. …”
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792por Jia, Qi, Liu, Na, Xie, Ke, Dai, Yanwan, Han, Shaojie, Zhao, Xijuan, Qian, Lichao, Wang, Yunjing, Zhao, Jinping, Gorovits, Rena, Xie, Daoxin, Hong, Yiguo, Liu, YuleEnlace del recurso
Publicado 2016
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793por Janda, Martin, Navrátil, Oldřich, Haisel, Daniel, Jindřichová, Barbora, Fousek, Jan, Burketová, Lenka, Čeřovská, Noemi, Moravec, Tomáš“…Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources. …”
Publicado 2015
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794por Ma, Haoran, Wang, Feng, Wang, Wenjing, Yin, Guoying, Zhang, Dingyu, Ding, Yongqiang, Timko, Michael P., Zhang, HongboEnlace del recurso
Publicado 2016
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795por Vega-Arreguín, Julio C., Shimada-Beltrán, Harumi, Sevillano-Serrano, Jacobo, Moffett, Peter“…Here, we have used virus-induced gene silencing (VIGS) and transgenic tobacco plants expressing dsRNA in Nicotiana spp. to identify candidate R genes that mediate non-host resistance to P. capsici. …”
Publicado 2017
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796por Brendolise, Cyril, Montefiori, Mirco, Dinis, Romain, Peeters, Nemo, Storey, Roy D., Rikkerink, Erik H.“…RESULTS: We present a novel method to identify R genes responsible for the recognition of specific effectors that trigger a hypersensitive response (HR) in Nicotiana benthamiana. This method is based on the genome-wide identification of most of the potential R genes of N. benthamiana and a systematic silencing of these potential R genes in a simple transient expression assay. …”
Publicado 2017
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797por Mazalovska, Milena, Varadinov, Nikola, Koynarski, Tsvetoslav, Minkov, Ivan, Teoharov, Pavel, Lomonossoff, George P., Zahmanova, Gergana“…RESULTS: We confirmed the transient expression and purification of a truncated version of the HEV genotype 3 capsid protein in Nicotiana benthamiana and its usefulness as a diagnostic antigen. …”
Publicado 2017
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798por Evangelisti, Edouard, Gogleva, Anna, Hainaux, Thomas, Doumane, Mehdi, Tulin, Frej, Quan, Clément, Yunusov, Temur, Floch, Kévin, Schornack, Sebastian“…RESULTS: We employed the model plant Nicotiana benthamiana to study the P. palmivora root infections at the cellular and molecular levels. …”
Publicado 2017
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799por Gomariz-Fernández, Africa, Sánchez-Gerschon, Verónica, Fourquin, Chloé, Ferrándiz, Cristina“…In this work, we have studied the functional conservation of transcription factors belonging to the SHI/STY/SRS family in two distant species within the eudicots, Eschscholzia californica and Nicotiana benthamiana. We have found that the expression patterns of EcSRS-L and NbSRS-L genes during flower development are similar to each other and to those reported for Arabidopsis SHI/STY/SRS genes. …”
Publicado 2017
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800por Vojta, Lea, Ljuma-Skupnjak, Lana, Budimir, Ankica, Vukičević, Slobodan, Fulgosi, HrvojeEnlace del recurso
Publicado 2015
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