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881por Pang, Ee Leen, Peyret, Hadrien, Ramirez, Alex, Loh, Hwei-San, Lai, Kok-Song, Fang, Chee-Mun, Rosenberg, William M., Lomonossoff, George P.“…The tandem core particles displaying cEDIII epitopes (tHBcAg-cEDIII) were successfully produced in Nicotiana benthamiana via Agrobacterium-mediated transient expression strategy to give a protein of ∼54 kDa, detected in both soluble and insoluble fractions of plant extracts. …”
Publicado 2019
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882por Yin, Xiao, Shang, Boxing, Dou, Mengru, Liu, Ruiqi, Chen, Tingting, Xiang, Gaoqing, Li, Yanzhuo, Liu, Guotian, Xu, Yan“…Here, we show that a candidate P. viticola RxLR effector, PvAvh74, induces cell death in Nicotiana benthamiana leaves. Using agroinfiltration, we found that nuclear localization, two putative N-glycosylation sites, and 427 amino acids of the PvAvh74 carboxyl terminus were necessary for cell-death-inducing activity. …”
Publicado 2019
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883por Bai, Ge, Yang, Da-Hai, Cao, Peijian, Yao, Heng, Zhang, Yihan, Chen, Xuejun, Xiao, Bingguang, Li, Feng, Wang, Zhen-Yu, Yang, Jun, Xie, He“…MADS-box genes play a pivotal role in various processes, including floral and seed development, controlling flowering time, regulation of fruits ripening, and respond to abiotic and biotic stressors in planta. Tobacco (Nicotiana tabacum) has been widely used as a model plant for analyzing the gene function, however, there has been less information on the regulation of flowering, and the associated genes. …”
Publicado 2019
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884por Qin, Yan, Bai, Shenglong, Li, Wenzheng, Sun, Ting, Galbraith, David W., Yang, Zefeng, Zhou, Yun, Sun, Guiling, Wang, Bingwu“…BACKGROUND: Nicotiana tabacum is an important economic crop. Topping, a common agricultural practice employed with flue-cured tobacco, is designed to increase leaf nicotine contents by increasing nicotine biosynthesis in roots. …”
Publicado 2020
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885por Jeon, Hyelim, Kim, Wanhui, Kim, Boyoung, Lee, Sookyeong, Jayaraman, Jay, Jung, Gayoung, Choi, Sera, Sohn, Kee Hoon, Segonzac, Cécile“…While 2 of these T3Es elicited cell death in both Nicotiana benthamiana and N. tabacum, only one was dependent on suppressor of G2 allele of skp1 (SGT1), a molecular chaperone of nucleotide-binding and leucine-rich repeat immune receptors. …”
Publicado 2020
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886por Wu, Shengjiang, Guo, Yushuang, Adil, Muhammad Faheem, Sehar, Shafaque, Cai, Bin, Xiang, Zhangmin, Tu, Yonggao, Zhao, Degang, Shamsi, Imran Haider“…Tobacco (Nicotiana tabacum), is a world’s major non-food agricultural crop widely cultivated for its economic value. …”
Publicado 2020
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888“…Here we investigated changes in physiology, hormones and transcriptomes in leaves of Nicotiana varieties K326 and Basma Xanthi with/without drought treatment at time courses spanning 1 month. …”
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889por Jeon, Hyelim, Kim, Wanhui, Kim, Boyoung, Lee, Sookyeong, Jayaraman, Jay, Jung, Gayoung, Choi, Sera, Sohn, Kee Hoon, Segonzac, CécileEnlace del recurso
Publicado 2020
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890por Silva, Fredy A., Chatt, Elizabeth C., Mahalim, Siti-Nabilla, Guirgis, Adel, Guo, Xingche, Nettleton, Daniel S., Nikolau, Basil J., Thornburg, Robert W.“…The nectar metabolomes of different Nicotiana species can predict the feeding preferences of the target pollinator(s) of each species, and the nectar sugars (i.e., glucose, fructose, and sucrose) are a distinguishing feature of Nicotiana species phylogeny. …”
Publicado 2020
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891por Rattanapisit, Kaewta, Shanmugaraj, Balamurugan, Manopwisedjaroen, Suwimon, Purwono, Priyo Budi, Siriwattananon, Konlavat, Khorattanakulchai, Narach, Hanittinan, Oranicha, Boonyayothin, Wanuttha, Thitithanyanont, Arunee, Smith, Duncan R., Phoolcharoen, Waranyoo“…In this study, we explored the possibility of producing the receptor binding domain (RBD) of SARS-CoV-2 and an anti-SARS-CoV monoclonal antibody (mAb) CR3022 in Nicotiana benthamiana. Both RBD and mAb CR3022 were transiently produced with the highest expression level of 8 μg/g and 130 μg/g leaf fresh weight respectively at 3 days post-infiltration. …”
Publicado 2020
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892“…In this study, we used a leaf-disk assay based on transient overexpression in Nicotiana benthamiana to allow functional screening of 10 candidate pathogenic proteins secreted by PWNs. …”
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893por Zahmanova, Gergana, Mazalovska, Milena, Takova, Katerina, Toneva, Valentina, Minkov, Ivan, Peyret, Hadrien, Lomonossoff, George“…The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. …”
Publicado 2021
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894por Shi, Sujuan, An, Lulu, Mao, Jingjing, Aluko, Oluwaseun Olayemi, Ullah, Zia, Xu, Fangzheng, Liu, Guanshan, Liu, Haobao, Wang, Qian“…In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. …”
Publicado 2021
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895por Huang, Maoxi, Yan, Yunlong, Wang, Li, Chen, Jun, Liu, Tao, Xie, Xin, Li, XiangyangEnlace del recurso
Publicado 2021
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896por Gutierrez-Albanchez, Enrique, García-Villaraco, Ana, Lucas, José A., Horche, Ignacio, Ramos-Solano, Beatriz, Gutierrez-Mañero, F. J.“…A novel Pseudomonas, designated strain BBB001(T), an aerobic, rod-shaped bacterium, was isolated from the rhizosphere of Nicotiana glauca in Las Palmas Gran Canaria, Spain. …”
Publicado 2021
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897por Dobrica, Mihaela‐Olivia, van Eerde, André, Tucureanu, Catalin, Onu, Adrian, Paruch, Lisa, Caras, Iuliana, Vlase, Ene, Steen, Hege, Haugslien, Sissel, Alonzi, Dominic, Zitzmann, Nicole, Bock, Ralph, Dubuisson, Jean, Popescu, Costin‐Ioan, Stavaru, Crina, Liu Clarke, Jihong, Branza‐Nichita, Norica“…Here we report the first evidence of successful production of the full‐length HCV E2 glycoprotein in Nicotiana benthamiana, by using the Agrobacterium‐mediated transient expression technology. …”
Publicado 2021
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898por Pérez-Cañamás, Miryam, Hevia, Elizabeth, Katsarou, Konstantina, Hernández, Carmen“…Previous work showed that Pelargonium line pattern virus (PLPV) is a very efficient inducer and target of RNA silencing as PLPV-infected Nicotiana benthamiana plants accumulate extraordinarily high amounts of vsRNAs that, strikingly, are independent of RDR6 activity. …”
Publicado 2021
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899por Pishchik, Veronika N., Filippova, Polina S., Mirskaya, Galina V., Khomyakov, Yuriy V., Vertebny, Vitaliy E., Dubovitskaya, Viktoriya I., Ostankova, Yuliya V., Semenov, Aleksandr V., Chakrabarty, Debasis, Zuev, Evgeny V., Chebotar, Vladimir K.“…Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their ability to improve the germination of wheat plants; they were identified as Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2. The protective effects of these epiphytic bacteria against Ni stress were studied in model experiments with two wheat cultivars: Ni stress-tolerant Leningradskaya 6 and susceptible Chinese spring. …”
Publicado 2021
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900por Bai, Ge, Yang, Da-Hai, Chao, Peijian, Yao, Heng, Fei, MingLiang, Zhang, Yihan, Chen, Xuejun, Xiao, Bingguang, Li, Feng, Wang, Zhen-Yu, Yang, Jun, Xie, HeEnlace del recurso
Publicado 2020
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