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921“…Here, we show that an N-terminal polyprotein fragment encoded by tomato torrado virus RNA1, located between the first ATG codon and the protease cofactor (ProCo) motif, induces an HR-like reaction in Nicotiana benthamiana. Agrobacterium tumefaciens-mediated transient expression of the first 105 amino acids (the calculated molecular weight of the fragment was ca. 11.33 kDa, hereafter refered to as the 11K domain) from ToTV RNA1 induced an HR-like phenotype in infiltrated leaves. …”
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922por Wilbers, Ruud H. P., Westerhof, Lotte B., van Raaij, Debbie R., van Adrichem, Marloes, Prakasa, Andreas D., Lozano‐Torres, Jose L., Bakker, Jaap, Smant, Geert, Schots, Arjen“…In this study, we investigated the possibility to transiently express active human TGF‐β1 in Nicotiana benthamiana plants. We successfully expressed mature TGF‐β1 in the absence of the latency‐associated peptide (LAP) using different strategies, but the obtained proteins were inactive. …”
Publicado 2016
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923“…In this study, we identify the genetic determinants that enable the mirid, Tupiocoris notatus, to feed on its well-defended host plant, Nicotiana attenuata, an outstanding model for plant-insect interaction studies. …”
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924“…As a consequence, some Nicotiana species invest large amounts of organic nitrogen for certain pollinators. …”
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925por Chen, Lijuan, Fei, Chunyan, Zhu, Lin, Xu, Zhenpeng, Zou, Wenshan, Yang, Ting, Lin, Honghui, Xi, Dehui“…The inoculation of Cucumber mosaic virus (CMV) in Nicotiana tabacum produced a commonly occurring sequence of classic patterns of DGIs and LGTs. …”
Publicado 2017
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927por Dickey, Alexia, Wang, Nan, Cooper, Edwin, Tull, Lauren, Breedlove, Drew, Mason, Hugh, Liu, Dehu, Wang, Kevin Yueju“…Both vectors were independently expressed in tobacco (Nicotiana tabacum) leaves transiently by agroinfiltration. …”
Publicado 2017
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928por Li, Yu, Wang, Ke, Lu, Qisen, Du, Juan, Wang, Zhenyue, Wang, Desen, Sun, Bingjian, Li, Honglian“…To evaluate the effect of in planta RNAi on the control of this nematode, a specific fragment from the protease gene, cathepsin S (Rs-cps), was cloned into the binary vector pFGC5941 in the forward and reverse orientations to construct recombinant plant RNAi vectors. Transgenic Nicotiana benthamiana plants expressing Rs-cps dsRNA were obtained and studied. …”
Publicado 2017
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929por Jin, Weihuan, Zhou, Qi, Wei, Yuanfang, Yang, Jinmiao, Hao, Fengsheng, Cheng, Zhipeng, Guo, Hongxiang, Liu, WeiqunEnlace del recurso
Publicado 2018
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930por Gedling, C. R., Ali, E. M., Gunadi, A., Finer, J. J., Xie, K., Liu, Y., Yoshikawa, N., Qu, F., Dorrance, A. E.“…RESULTS: In this report, the ALSV VIGS vector was modified by mobilizing ALSV cDNAs into a binary vector compatible with Agrobacterium tumefaciens-mediated delivery, so that VIGS-triggering ALSV variants could be propagated in agro-infiltrated Nicotiana benthamiana leaves. Homogenate of these N. benthamiana leaves was then applied directly onto the unifoliate of young soybean seedlings to initiate systemic gene silencing. …”
Publicado 2018
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931por Liang, Yingbo, Cui, Shichun, Tang, Xiaoli, Zhang, Yi, Qiu, Dewen, Zeng, Hongmei, Guo, Lihua, Yuan, Jingjing, Yang, Xiufen“…Here, AtNRP homolog in Nicotiana benthamiana was identified and designated as Nbnrp1. …”
Publicado 2018
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932por Sui, Jinkai, Wang, Chunkai, Liu, Xiaofeng, Fang, Ning, Liu, Yanhua, Wang, Wenjing, Yan, Ning, Zhang, Huai-Bao, Du, Yongmei, Liu, Xinmin, Lu, Tiegang, Zhang, Zhongfeng, Zhang, Hongbo“…Cembranoids are a group of natural diterpenoid compounds with pharmaceutical potentials, and the cembratriene-diols produced by Nicotiana (tobacco) species display activities in anti-nicotine addiction and neuron protection. …”
Publicado 2018
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933por Xia, Fei, Sun, Tingting, Yang, Shuangjuan, Wang, Xiao, Chao, Jiangtao, Li, Xiaoxu, Hu, Junhua, Cui, Mengmeng, Liu, Guanshan, Wang, Dawei, Sun, YuheEnlace del recurso
Publicado 2019
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934“…However, it is not clear whether PLPs are involved in Nicotiana attenuata resistance against the necrotrophic fungal pathogen, Alternaria alternata. …”
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935por Matsumura, Emilyn E., Coletta‐Filho, Helvécio D., Machado, Marcos A., Nouri, Shahideh, Falk, Bryce W.“…Agrobacterium tumefaciens‐mediated inoculation of 35SRbz‐CSDaV in Nicotiana benthamiana plants enabled a fast recovery of large amounts of virions from the agroinfiltrated leaves, which allowed a better molecular characterization of CSDaV. …”
Publicado 2019
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936por Fay, Petra C., Attoui, Houssam, Batten, Carrie, Mohd Jaafar, Fauziah, Lomonossoff, George P., Daly, Janet M., Mertens, Peter P.C.“…His-tagged VP2 proteins of BTV serotypes 4 and 8 were transiently expressed in Nicotiana benthamiana then purified by immobilised metal affinity chromatography (IMAC). …”
Publicado 2019
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937“…Mutagenesis showed that PSTVd genomes containing base substitutions in loop 27 predicted by JAR3D to disrupt the 3D structure were unable to replicate in Nicotiana benthamiana leaves following mechanical rub inoculation, with one exception: a U178G/U179G double mutant was replication-competent and able to spread within the upper epidermis of inoculated leaves, but was confined to this cell layer. …”
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938por Song, Liyun, Wang, Jie, Jia, Haiyan, Kamran, Ali, Qin, Yuanxia, Liu, Yingjie, Hao, Kaiqiang, Han, Fei, Zhang, Chaoqun, Li, Bin, Li, Yongliang, Shen, Lili, Wang, Fenglong, Wu, Yuanhua, Yang, Jinguang“…As the type species of widely distributed and economically devastating Potyvirus, Potato virus Y (PVY) is one of the major constraints to important crop plants including tobacco (Nicotiana benthamiana) worldwide. Despite the great losses owing to PVY infection in tobacco, there is no previous study investigating the potential role of MLPs in developing resistance to viral infection. …”
Publicado 2020
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939“…We identified 59 members of the tobacco (Nicotiana tabacum L.) NtVQ gene family by in silico analysis and examined their differential expression in response to phytohormonal treatments and following exposure to biotic and abiotic stressors. …”
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940por Khezri, Ghaffar, Baghban Kohneh Rouz, Bahram, Ofoghi, Hamideh, Davarpanah, Seyed Javad“…In the present study, the Nicotiana benthamiana codon optimized Mambalgin-1 gene was synthesized and cloned in PVX (potato virus X) viral vector. …”
Publicado 2020
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