-
1101“…The HBcAg-wDIII fusion protein was quickly produced in Nicotiana benthamiana plants and reached a high expression level of approximately 1.2 mg of fusion protein per gram of leaf fresh weight within six days post gene infiltration. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1102por Zhou, Yang, Ding, Meiqi, Duan, Xiaodong, Konrad, Kai R., Nagel, Georg, Gao, Shiqiang“…Lately, we have expanded this technique into plant science by co-expression of a chloroplast-targeted β-carotene dioxygenase and an improved anion channelrhodopsin GtACR1 from the green alga Guillardia theta. The growth of Nicotiana tabacum pollen tube can then be manipulated by localized green light illumination. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1103“…The present study aimed to identify the function and mechanism of RDR1 in plant resistance to viroid infection. Overexpression of Nicotiana tabacum RDR1 (NtRDR1) delayed the accumulation of potato spindle tuber viroid (PSTVd) genomic RNA and PSTVd‐derived small RNA (sRNA) in Nicotiana benthamiana plants at the early invasion stage, but not in the late stage of infection. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1104“…We overexpressed NbTFIIIA-7ZF from Nicotiana benthamiana in tobacco (Nicotiana tabacum) where it caused morphological and physiological deviations like plant stunting, splitting of leaf petioles, pistils or apexes, irregular branching of shoots, formation of double-blade leaves, deformation of main stems, and modification of glandular trichomes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1105“…Transient expression in Nicotiana benthamiana showed that CsMIXTA is localized in the nucleus. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1106por Wang, Xu, Prokhnevsky, Alexei I., Skarjinskaia, Marina, Razzak, Md Abdur, Streatfield, Stephen J., Lee, Jung‐Youn“…Molecular farming technology using transiently transformed Nicotiana plants offers an economical approach to the pharmaceutical industry to produce an array of protein targets including vaccine antigens and therapeutics. …”
Publicado 2023
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1107por MacDonald, Jacqueline, Miletic, Sean, Gaildry, Typhanie, Chin-Fatt, Adam, Menassa, Rima“…Toward developing a recombinant vaccine against enterohaemorrhagic E. coli (EHEC), we expressed recombinant T3SS and related proteins from predominant EHEC serotypes in Nicotiana chloroplasts. Nicotiana benthamiana were transiently transformed to express chloroplast-targeted Tir, NleA, and EspD from the EHEC serotype O157:H7; a fusion of EspA proteins from serotypes O157:H7 and O26:H11; and a fusion of epitopes of Tir (Tir-ep) from serotypes O157:H7, O26:H11, O45:H2, and O111:H8. …”
Publicado 2017
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1108“…Tnt1 (transposable element if Nicotiana tabaccum cell type 1) is one of the very few active LTR retrotransposons used for gene tagging in plants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1109“…Plant ploidy was weakly negatively correlated with virus resistance in Nicotiana species, but this trend did not hold when synthetic allopolyploids were taken into account. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1110por Schoberer, Jennifer, Liebminger, Eva, Vavra, Ulrike, Veit, Christiane, Castilho, Alexandra, Dicker, Martina, Maresch, Daniel, Altmann, Friedrich, Hawes, Chris, Botchway, Stanley W, Strasser, Richard“…Here, we used a series of chimeric proteins to investigate the contribution of the cytoplasmic, transmembrane and stem region of Nicotiana benthamiana N–acetylglucosaminyltransferase I (GnTI) for its cis/medial-Golgi localization and for protein–protein interaction in the Golgi. …”
Publicado 2014
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1111por Gonzalez, Tania L., Liang, Yan, Nguyen, Bao N., Staskawicz, Brian J., Loqué, Dominique, Hammond, Ming C.“…This alternative splicing-based gene regulation system was shown to successfully control Bs2/AvrBs2-dependent and RPP1/ATR1Δ51-dependent HR in Nicotiana benthamiana and Nicotiana tabacum, respectively. …”
Publicado 2015
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1112por Adkar-Purushothama, Charith Raj, Kasai, Atsushi, Sugawara, Kohei, Yamamoto, Hideki, Yamazaki, Yuto, He, Ying-Hong, Takada, Nobuyuki, Goto, Hideki, Shindo, Sahori, Harada, Takeo, Sano, Teruo“…Therefore, in the present work, transgenic Nicotiana benthamiana lines expressing both partial and truncated versions of PSTVd hpRNA were developed. …”
Publicado 2015
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1113por Velasco-Medina, Carlos, Espinoza-Montero, Patricio J., Montero-Jimenez, Marjorie, Alvarado, José, Jadán, Mónica, Carrera, Patricio, Fernandez, Lenys“…To check the accuracy of the electrodes, real samples of Nicotiana tabacum cells exposed to cytotoxic levels of cadmium were tested. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1114“…To identify R. solanacearum effectors that suppress pattern-triggered immunity (PTI) in plants, we transiently expressed R. solanacearum RS1000 effectors in Nicotiana benthamiana leaves and evaluated their ability to suppress the production of reactive oxygen species (ROS) triggered by flg22. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1115por Md. Ali, Emran, Kobayashi, Kappei, Yamaoka, Naoto, Ishikawa, Masayuki, Nishiguchi, Masamichi“…Non-transgenic Nicotiana tabacum (cvs. Samsun and Xanthi nc) and N. benthamiana were used as scions for grafting onto the rootstocks silenced with both genes. …”
Publicado 2013
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1116“…The heavy and light chain genes of VRC01 were cloned onto a single vector, pTRAk.2, which was transformed into Nicotiana benthamiana or Nicotiana tabacum using transient and stable expression production systems respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1117por Rademacher, Thomas, Sack, Markus, Blessing, Daniel, Fischer, Rainer, Holland, Tanja, Buyel, Johannes“…This approach is compatible with different plant species such as Nicotiana tabacum BY2, Nicotiana benthamiana or Daucus carota and 10‐times more effective than transient expression in liquid plant cell culture. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1118por Rahman, Hafizur, Fukushima, Chika, Kaya, Hidetaka, Yaeno, Takashi, Kobayashi, Kappei“…A Cas9 gene was introduced into Nicotiana tabacum and Nicotiana sylvestris together with an sgRNA gene for one of three different target sequences designed to perfectly match sequences in both S- and T-genome copies of N. tabacum MAR1 homologs (NtMAR1hs). …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1119por Ihara, Yuta, Wakamatsu, Takayuki, Yokoyama, Mineyuki, Maezawa, Daisuke, Ohta, Hiroyuki, Shimojima, Mie“…To improve KODA biosynthetic yield in other plants such as Nicotiana benthamiana and Arabidopsis thaliana, we developed a system to overproduce KODA in vivo via ectopic expression of L. paucicostata 9-LOX and AOS. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
1120por Leibman-Markus, Meirav, Schuster, Silvia, Vasquez-Soto, Beatriz, Bar, Maya, Avni, Adi, Pizarro, Lorena“…Through transient expression of SlDRP1B and its dominant-negative mutant on Nicotiana benthamiana and Nicotiana tabacum, we analyzed SlDRP1B function. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto