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  1. 1741
    “…Consistent with previous observations in plants, the loop sequence of long hpRNA expressed in Agrobacterium-infiltrated Nicotiana benthamiana leaves was excised, leaving no detectable levels of full-length hpRNA molecule. …”
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    Full-Length Hairpin RNA Accumulates at High Levels in Yeast but Not in Bacteria and Plants
  2. 1742
    “…Further analysis shows that RipN localizes to the endoplasmic reticulum (ER) and nucleus in Nicotiana tabacum leaf cells and Arabidopsis protoplasts, and truncation of the C‐terminus of RipN results in a loss of nuclear and ER targeting. …”
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    The Ralstonia solanacearum effector RipN suppresses plant PAMP‐triggered immunity, localizes to the endoplasmic reticulum and nucleus, and alters the NADH/NAD(+) ratio in Arabidopsis
  3. 1743
    “…The combined use of recent high‐throughput methods for artificial sRNA construct generation and the Potato spindle tuber viroid (PSTVd)–Nicotiana benthamiana pathosystem allowed for the simple and time‐effective screening of multiple artificial sRNAs targeting sites distributed along PSTVd RNAs of (+) or (–) polarity. …”
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    Artificial microRNAs and synthetic trans‐acting small interfering RNAs interfere with viroid infection
  4. 1744
    “…In this study, the PCV‐2 CP was transiently expressed in Nicotiana benthamiana plants via agroinfiltration and PCV‐2 CP was successfully purified using sucrose gradient ultracentrifugation. …”
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    Immunogenicity of plant‐produced porcine circovirus‐like particles in mice
  5. 1745
    “…The aim of this study was to evaluate the phytotoxic effects of Eucalyptus citriodora, Lavandula angustifolia, and Pinus sylvestris, three common commercial essential oils, on weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli), food crops (tomato and cucumber), and the invasive species Nicotiana glauca. Methods: to determine herbicidal effects, essential oils were tested at different concentrations (0.125–1 µL/mL). …”
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    Phytotoxic Effects of Commercial Eucalyptus citriodora, Lavandula angustifolia, and Pinus sylvestris Essential Oils on Weeds, Crops, and Invasive Species
  6. 1746
    “…In this study, we explore the function of auxin and phosphate (P) in tobacco (Nicotiana tabacum), with particular focus on their interaction with Se and Cd. …”
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    Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
  7. 1747
    “…Fluorescent signals from the eYFP:PpGPAT9 construct were observed in the ER of Nicotiana benthamiana leaf epidermal cells. Ectopic expression of PpGPAT9 increased the seed oil content by approximately 10% in Arabidopsis. …”
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    Functional Characterization of Physcomitrella patens Glycerol-3-Phosphate Acyltransferase 9 and an Increase in Seed Oil Content in Arabidopsis by Its Ectopic Expression
  8. 1748
    “…Additionally, we found that the full and partial sequences of BEC1019 can trigger cell death in Nicotiana benthamiana leaves. These results indicate that Bgt and Ggt can utilize BEC1019 as a virulence effector to promote plant colonization, and thus these genes represent promising new targets in breeding wheat cultivars with broad-spectrum resistance.…”
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    The Highly Conserved Barley Powdery Mildew Effector BEC1019 Confers Susceptibility to Biotrophic and Necrotrophic Pathogens in Wheat
  9. 1749
    “…MsGSTU8, from alfalfa (Medicago sativa ‘Zhaodong’), was transformed into transgenic tobacco (Nicotiana tabacum) and overexpressed to determine its effects on saline-alkali tolerance. …”
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    Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
  10. 1750
    “…Proteomics analysis of Nicotiana benthamiana leaves expressing p22, and its F-box-like motif deletion mutant showed 228 differentially expressed proteins and five enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: ABC transporters, sesquiterpenoid and triterpenoid biosynthesis, ubiquitin-mediated proteolysis, riboflavin metabolism, and cysteine and methionine metabolism. …”
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    Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
  11. 1751
    “…Overexpression of CsBPC2 in tobacco (Nicotiana tabacum) inhibited seed germination under saline, polyethylene glycol, and abscisic acid (ABA) conditions. …”
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    Genome-Wide Identification and Characterization of Cucumber BPC Transcription Factors and Their Responses to Abiotic Stresses and Exogenous Phytohormones
  12. 1752
    “…Both the A. thaliana T‐DNA insertion rtp5 mutants and the Nicotiana benthamiana RTP5 (NbRTP5) silencing plants showed enhanced resistance to P. parasitica, while overexpression of AtRTP5 rendered plants more susceptible. …”
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    AtRTP5 negatively regulates plant resistance to Phytophthora pathogens by modulating the biosynthesis of endogenous jasmonic acid and salicylic acid
  13. 1753
    “…Expression of translational fusion proteins with a GFP reporter in Nicotiana benthamiana leaves confirmed the ER- and plasma membrane-specific localization, suggesting the role of ClAAP proteins in the cellular import of amino acids. …”
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    Broad-Spectrum Amino Acid Transporters ClAAP3 and ClAAP6 Expressed in Watermelon Fruits
  14. 1754
    “…A bimolecular fluorescence complementation (BIFC) assay displayed that the subcellular distribution patterns of TaLIP were altered by NIb in Nicotiana benthamiana. Transcription of TaLIP was significantly decreased by WYMV infection and TaLIP-silencing wheat plants displayed more susceptibility to WYMV in comparison with the control plants, suggesting that knockdown of TaLIP impaired host resistance. …”
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    Wheat Yellow Mosaic Virus NIb Interacting with Host Light Induced Protein (LIP) Facilitates Its Infection through Perturbing the Abscisic Acid Pathway in Wheat
  15. 1755
    “…Fluorescent protein-tagged full length formins and their individual domains were transiently expressed in Nicotiana benthamiana leaves under the control of a constitutive promoter and their subcellular localization (including co-localization with cytoskeletal structures and the endoplasmic reticulum) was examined using confocal microscopy. …”
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    Arabidopsis Class II Formins AtFH13 and AtFH14 Can Form Heterodimers but Exhibit Distinct Patterns of Cellular Localization
  16. 1756
    “…Transgenic tobacco (Nicotiana tabacum) expressing AcFLS-HRB produced lighter-pink flowers compared to wild-type plants. …”
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    Increased Flavonol Levels in Tobacco Expressing AcFLS Affect Flower Color and Root Growth
  17. 1757
    “…When the RNAi constructs of VdTHI20 were used to transform Nicotiana benthamiana, the transgenic lines expressing dsVdTHI20 showed elevated resistance to V. dahliae. …”
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    Verticillium dahliae VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity
  18. 1758
    “…The hybrid protein was expressed in Nicotiana benthamiana plants using the self-replicating potato virus X-based vector pEff up to 300 µg/g of fresh leaf tissue. …”
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    Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin
  19. 1759
    “…To better understand this interaction, fluorescent PBs, resulting from the fusion of the N‐terminal portion of zein to a green fluorescent protein, was produced in Nicotiana benthamiana leaves, recovered by a filtration‐based downstream procedure, and used to investigate their internalization efficiency into mammalian cells. …”
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    Plant‐derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells
  20. 1760
    por Park, Ky Young, Wi, Soo Jin
    Publicado 2016
    “…A cocktail of three monoclonal antibodies was produced in tobacco (Nicotiana benthamiana) plants (Chen and Davis 2016). …”
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    Potential of plants to produce recombinant protein products
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