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1881por Chichester, Jessica A., Jones, R. Mark, Green, Brian J., Stow, Mark, Miao, Fudu, Moonsammy, George, Streatfield, Stephen J., Yusibov, Vidadi“…Recently, we have reported [1,2] on a subunit influenza vaccine candidate based on the recombinant hemagglutinin protein from the A/Indonesia/05/2005 (H5N1) strain of influenza virus, produced it using ‘launch vector’-based transient expression technology in Nicotiana benthamiana, and demonstrated its immunogenicity in pre-clinical studies. …”
Publicado 2012
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1882por Gushchin, Vladimir A., Solovyev, Andrey G., Erokhina, Tatyana N., Morozov, Sergey Y., Agranovsky, Alexey A.“…Transient expression of a hydrophobic segment (referred to here as CR-2) of the BYV 1a in Nicotiana benthamiana led to reorganization of the ER and formation of ~1-μm mobile globules. …”
Publicado 2013
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1883por Stam, Remco, Jupe, Julietta, Howden, Andrew J. M., Morris, Jenny A., Boevink, Petra C., Hedley, Pete E., Huitema, Edgar“…Phenotypic analyses showed that few CRN domains induce necrosis when expressed in planta and that one cell death inducing effector, enhances P. capsici virulence on Nicotiana benthamiana. These results suggest that the CRN protein family form an important class of intracellular effectors that target the host nucleus during infection. …”
Publicado 2013
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1884por Park, Hyun Ji, Lee, Sang Sook, You, Young Nim, Yoon, Dae Hwa, Kim, Beom-Gi, Ahn, Jun Cheul, Cho, Hye Sun“…The chloroplast localization of OsFKBP16-3-GFP was confirmed through the transient expression of OsFKBP16-3 in Nicotiana benthamiana leaves. Transgenic Arabidopsis and transgenic rice plants that constitutively expressed OsFKBP16-3 exhibited increased tolerance to salinity, drought and oxidative stresses, but showed no change in growth or phenotype, compared with vector control plants, when grown under non-stressed conditions. …”
Publicado 2013
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1885“…Ectopic expression of PaAG-1 in tobacco (Nicotiana tabacum) resulted in morphological alterations of the outer two flower whorls, as well as some defects in vegetative growth. …”
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1886por Wang, Chen, Deng, Pengyi, Chen, Liulin, Wang, Xiatian, Ma, Hui, Hu, Wei, Yao, Ningcong, Feng, Ying, Chai, Ruihong, Yang, Guangxiao, He, Guangyuan“…Overexpression of TaWRKY10 in tobacco (Nicotiana tabacum L.) resulted in enhanced drought and salt stress tolerance, mainly demonstrated by the transgenic plants exhibiting of increased germination rate, root length, survival rate, and relative water content under these stress conditions. …”
Publicado 2013
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1887“…BACKGROUND: The tobacco plant, Nicotiana tabacum, has been responsible for more deaths than any other herb. …”
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1888“…A study was conducted on tobacco (Nicotiana tabacum) plants for altered rates of biosynthesis, catabolism, and GA signalling constitutively or in specific tissues using a transgenic approach. …”
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1889“…Ectopic expression in Nicotiana benthamiana showed that P. capsici RXLR and Crinkler effectors can suppress host cell death triggered by diverse elicitors including P. capsici elicitin and NLP effectors. …”
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1890por Khan, Arif Muhammad, Ashfaq, Muhammad, Kiss, Zsofia, Khan, Azhar Abbas, Mansoor, Shahid, Falk, Bryce W.“…We also used recombinant Tobacco mosaic virus (TMV) to express these RNAi effectors in Nicotiana benthamiana plants. We found that P. citri showed lower fecundity and pronounced death of crawlers after feeding on recombinant TMV-infected plants. …”
Publicado 2013
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1891“…To test this hypothesis in vivo, virus-induced gene silencing was used to reduce the expression of three PPR proteins and RNase J, both individually and jointly, in Nicotiana benthamiana. In accordance with the stability-conferring function of the PPR proteins PPR10, HCF152 and MRL1, accumulation of the cognate RNA species atpH, petB and rbcL was reduced when the PPR-encoding genes were silenced. …”
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1892por Jones, R. Mark, Chichester, Jessica A., Mett, Vadim, Jaje, Jennifer, Tottey, Stephen, Manceva, Slobodanka, Casta, Louis J., Gibbs, Sandra K., Musiychuk, Konstantin, Shamloul, Moneim, Norikane, Joey, Mett, Valentina, Streatfield, Stephen J., van de Vegte-Bolmer, Marga, Roeffen, Will, Sauerwein, Robert W., Yusibov, Vidadi“…We engineered VLPs (Pfs25-CP VLP) comprising Pfs25 fused to the Alfalfa mosaic virus coat protein (CP) and produced these non-enveloped hybrid VLPs in Nicotiana benthamiana plants using a Tobacco mosaic virus-based ‘launch’ vector. …”
Publicado 2013
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1893por Feller, Tatjana, Thom, Pascal, Koch, Natalie, Spiegel, Holger, Addai-Mensah, Otchere, Fischer, Rainer, Reimann, Andreas, Pradel, Gabriele, Fendel, Rolf, Schillberg, Stefan, Scheuermayer, Matthias, Schinkel, Helga“…In this study, we produced and purified recombinant Pf38 and a fusion protein composed of red fluorescent protein and Pf38 (RFP-Pf38) using a transient expression system in the plant Nicotiana benthamiana. To our knowledge, this is the first description of the production of recombinant Pf38. …”
Publicado 2013
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1894Expression and Biological Activity of the Cystine Knot Bioinsecticide PA1b (Pea Albumin 1 Subunit b)por Eyraud, Vanessa, Karaki, Lamis, Rahioui, Isabelle, Sivignon, Catherine, Da Silva, Pedro, Rahbé, Yvan, Royer, Corinne, Gressent, Frédéric“…In this paper, we present our results for the transient production of the peptide in Nicotiana benthamiana by agro-infiltration, with a yield of about 35 µg/g of fresh leaves and maximum production 8 days after infiltration. …”
Publicado 2013
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1895“…It was also revealed that 3-NO(2)-Tyr intensively decorates such highly dynamic microtubular arrays as preprophase bands, mitotic spindles and phragmoplasts of Nicotiana tabacum Bright Yellow-2 (BY-2) cells under physiological conditions. …”
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1896“…Over-expression of the Arabidopsis Pah2p in Nicotiana benthamiana decreased tombusvirus accumulation, validating that our findings are also relevant in a plant host. …”
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1897por Petukhova, Natalia V., Gasanova, Tatiana V., Ivanov, Peter A., Atabekov, Joseph G.“…Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides, and two point amino acid substitutions in recombinant CPs significantly influenced the symptoms and development of viral infections. …”
Publicado 2014
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1898“…Osmotin, a homolog of mammalian adiponectin, is a plant protein from Nicotiana tabacum that was examined for the first time in the present study to determine its protective effects against glutamate-induced synaptic dysfunction and neurodegeneration in the rat brain at postnatal day 7. …”
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1899“…This study used this technique to estimate b in vivo in five tobacco (Nicotiana tabacum L. cv Petit Havana [N,N]) genotypes expressing alternative Rubisco isoforms. …”
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1900por Noirot, Elodie, Der, Christophe, Lherminier, Jeannine, Robert, Franck, Moricova, Pavla, Kiêu, Kiên, Leborgne-Castel, Nathalie, Simon-Plas, Françoise, Bouhidel, Karim“…The subcellular localization of the tobacco (Nicotiana tabacum) ROS-producing enzyme RBOHD was examined in Bright Yellow-2 cells before and after elicitation with the oomycete protein cryptogein using electron and confocal microscopy. …”
Publicado 2014
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