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1961por Liu, Qing, Beyraghdar Kashkooli, Arman, Manzano, David, Pateraki, Irini, Richard, Lea, Kolkman, Pim, Lucas, Maria Fátima, Guallar, Victor, de Vos, Ric C.H., Franssen, Maurice C. R., van der Krol, Alexander, Bouwmeester, Harro“…The full kauniolide biosynthesis pathway is reconstructed in the heterologous hosts Nicotiana benthamiana and yeast, paving the way for biotechnological production of guaianolide-type sesquiterpene lactones.…”
Publicado 2018
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1962por Webster, Gina R., van Dolleweerd, Craig, Guerra, Thais, Stelter, Szymon, Hofmann, Sven, Kim, Mi‐Young, Teh, Audrey Y‐H., Diogo, Gil Reynolds, Copland, Alastair, Paul, Mathew J., Hart, Peter, Reljic, Rajko, Ma, Julian K‐C.“…Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana. The recombinant protein assembled into polymeric structures (TB‐PIGS) in N. benthamiana, similar in size to polymeric IgM. …”
Publicado 2018
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1963por Cai, Congxi, Yuan, Wenxin, Miao, Huiying, Deng, Mingdan, Wang, Mengyu, Lin, Jiayao, Zeng, Wei, Wang, Qiaomei“…To further investigate the transcriptional mechanism, we identified the transcriptional activation domain (TAD) and putative interacting proteins of BoaMYB51s by means of yeast (Saccharomyces cerevisiae) two hybrid. Using tobacco (Nicotiana benthamiana) transient expression assay, we confirmed that the carboxy-end is required for transcriptional activation activity of BoaMYB51s. …”
Publicado 2018
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1964“…An LIYV P26 knockout mutant (P26X) showed viral RNA replication and virion formation in inoculated leaves of Nicotiana benthamiana plants, but failed to give systemic infection. …”
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1965por Ye, Xiefeng, Ling, Tianxiao, Xue, Yanfeng, Xu, Cunfa, Zhou, Wei, Hu, Liangbin, Chen, Jian, Shi, Zhiqi“…In the present study physiological, histochemical, and biochemical methods were applied to investigate thymol-induced Cd tolerance in tobacco (Nicotiana tabacum) seedlings. Thymol was able to alleviate Cd-induced growth inhibition of tobacco seedlings in both dose- and time-dependent manners. …”
Publicado 2016
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1966por Lim, Sherene Swee Yin, Chua, Kek Heng, Nölke, Greta, Spiegel, Holger, Goh, Wai Leong, Chow, Sek Chuen, Kee, Boon Pin, Fischer, Rainer, Schillberg, Stefan, Othman, Rofina Yasmin“…This strategy yielded four mutated scFv sequences and a parental scFv that were used to produce five mouse–human chimeric IgGs in Nicotiana benthamiana plants, with yields of 33–72 mg/kg of plant tissue. …”
Publicado 2018
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1967por Wang, Ling, Liu, Feng, Zhang, Xu, Wang, Wenju, Sun, Tingting, Chen, Yufeng, Dai, Mingjian, Yu, Shengxiao, Xu, Liping, Su, Yachun, Que, Youxiong“…The ScWRKY3 protein was localized in the nucleus of Nicotiana benthamiana leaves and showed no transcriptional activation activity and no toxic effects on the yeast strain Y2HGold. …”
Publicado 2018
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1968“…This complex was taken up via the hydathodes in Arabidopsis thaliana, and from root hairs in Nicotiana benthamiana. Remarkably, these structurally weak tissues are also routes of bacterial invasion in nature, suggesting that peptide-pDNA complexes invade intact plants through similar pathways as bacterial pathogens.…”
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1969“…In addition, we present a CRISPR-based methylation targeting system for plants, utilizing a SunTag system with the catalytic domain of the Nicotiana tabacum DRM methyltransferase, which efficiently targets DNA methylation to specific loci, including the FWA promoter, triggering a developmental phenotype, and the SUPERMAN promoter. …”
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1970por Rashid, Mamun-Or, Zhang, Xiao-Yan, Wang, Ying, Li, Da-Wei, Yu, Jia-Lin, Han, Cheng-Gui“…The RT-PCR and western blot analysis revealed that these defective mutants affected virus accumulation in inoculated leaves and systemic movement in Nicotiana benthamiana as well as in its natural hosts, potato and black nightshade. …”
Publicado 2019
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1971por Bi, Ji’an, Yang, Yong, Chen, Binghua, Zhao, Jinping, Chen, Zhuo, Song, Baoan, Chen, Jianping, Yan, Fei“…Under laboratory conditions, RSV can infect Nicotiana benthamiana by mechanical inoculation, providing a useful system to study RSV–plant interactions. …”
Publicado 2019
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1972por Jiang, Ning, Zhang, Chao, Liu, Jun‐Ying, Guo, Zhi‐Hong, Zhang, Zong‐Ying, Han, Cheng‐Gui, Wang, Ying“…We further developed a set of BNYVV‐based vectors that permit efficient expression of four recombinant proteins, including some large proteins with lengths up to 880 amino acids in the model plant Nicotiana benthamiana and native host sugar beet plants. …”
Publicado 2019
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1973“…Here, we present a novel model for photosynthesis research in which alien henbane (Hyoscyamus niger) chloroplasts function on the nuclear background of a tobacco (Nicotiana tabacum). The result of this coupling is a cytoplasmic hybrid (cybrid) with inhibited state transitions—a mechanism responsible for balancing energy absorption between photosystems. …”
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1974“…A subcellular localization analysis of green fluorescent protein (GFP) fusion reveals that CsCaM3 is transferred from the cytoplasm to the plasma membrane in Nicotiana benthamiana, and CsCaM3 green fluorescence is significantly attenuated via the coexpression of CsMLO1 and CsCaM3. …”
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1975“…In central Australia, Aboriginal populations utilize wild tobacco plants (Nicotiana spp.) as a smokeless product. These plants are known by a variety of names, one of which is pituri. …”
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1976por Jin, Jing-Hao, Zhang, Huai-Xia, Ali, Muhammad, Wei, Ai-Min, Luo, De-Xu, Gong, Zhen-Hui“…Results revealed that the N-terminal, AP2 domain, and C-terminal of CaAP2/ERF064 protein is responsible for triggering cell death in Nicotiana benthamiana (N. benthamiana). Moreover, the transcription of CaAP2/ERF064 in plant is synergistically regulated by the Methyl-Jasmonate (MeJA) and ethephon (ET) signaling pathway. …”
Publicado 2019
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1977por Dong, Haiqiang, Liu, Lanxin, Fan, Xingqiang, Asghar, Sumeera, Li, Yi, Wang, Yi, Xu, Xuefeng, Wu, Ting, Zhang, Xinzhong, Qiu, Changpeng, Han, Zhenhai, Li, Wei“…When transferred into tobacco (Nicotiana benthamiana) by Agrobacterium tumefaciens-mediated stable transformation, one promoter, rMdAG2I, exhibited activity specifically in flowers, whereas the other three also showed detectable activity in vegetative organs. …”
Publicado 2019
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1978por Huang, Dong, Wang, Qian, Duan, Dingyue, Dong, Qinglong, Zhao, Shuang, Zhang, Maoxue, Jing, Guangquan, Liu, Changhai, van Nocker, Steve, Ma, Fengwang, Li, Chao“…The overexpression of MdIAA9 in tobacco (Nicotiana tabacum L.) plants significantly increased their tolerance to osmotic stresses. …”
Publicado 2019
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1979“…The transient expression experiment in Nicotiana tabacum leaves and Actinidia arguta fruit indicated the interaction of AcMYB10 with AcbHLH42 might strongly activate anthocyanin biosynthesis by activating the transcription of AcLDOX and AcF3GT. …”
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1980“…Transcriptional level evidence indicates that Fg-sRNA1 can target TaCEBiP mRNA and trigger silencing of TaCEBiP in vivo, and in Nicotiana benthamiana (N. benthamiana) plants, Western blotting experiments and YFP Fluorescence observation proofs show that Fg-sRNA1 can suppress the accumulation of protein coding by TaCEBiP gene in vitro. …”
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