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1141por Karpov, Dmitry S., Goncharenko, Anna V., Usachev, Evgenii V., Vasina, Daria V., Divisenko, Elizaveta V., Chalenko, Yaroslava M., Pochtovyi, Andrei A., Ovchinnikov, Roman S., Makarov, Valentin V., Yudin, Sergei M., Tkachuk, Artem P., Gushchin, Vladimir A.“…As a result, we created a synthetic chromoprotein-expressing reporter operon. This operon was used to improve the V. cholerae genome engineering approach and monitor the stability of the genetic constructs. …”
Publicado 2021
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1142“…Transcription of the potABCD operon is induced by peroxide-induced oxidative stress but not by acidic stress. …”
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1143por Chandra, Soumyanetra, Gupta, Kritika, Khare, Shruti, Kohli, Pehu, Asok, Aparna, Mohan, Sonali Vishwa, Gowda, Harsha, Varadarajan, Raghavan“…Thus, in their operonic context, genes are likely to be more sensitive to both synonymous and nonsynonymous point mutations than inferred previously.…”
Publicado 2022
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1144por Sourice, Mathieu, Askenasy, Isabel, Garcia, Pierre Simon, Denis, Yann, Brasseur, Gaël, Kiley, Patricia J., Py, Béatrice, Aubert, Corinne“…This transcriptional response is directed by RisR, which we show to belong to a phylogenetically distant and biochemically distinct subgroup of the Rrf2 transcription factor family, in comparison to IscR that regulates the isc and suf operons in Enterobacteria. We report that RisR harbors an Fe-S cluster and that holo-RisR acts as a repressor of both the isc and suf operons, in contrast to Escherichia coli, where holo-IscR represses the isc operon whereas apo-IscR activates the suf operon. …”
Publicado 2023
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1145por Fan, Zheng, Fu, Tongtong, Li, Zhoufei, Du, Bing, Cui, Xiaohu, Zhang, Rui, Feng, Yanling, Zhao, Hanqing, Xue, Guanhua, Cui, Jinghua, Yan, Chao, Gan, Lin, Feng, Junxia, Xu, Ziying, Yu, Zihui, Tian, Ziyan, Ding, Zanbo, Chen, Jinfeng, Chen, Yujie, Yuan, Jing“…RNA sequencing differential gene expression analysis showed that compared with the wild-type control, the expression of many virulence factor genes was downregulated in ΔihfA and ΔihfB strains, such as those related to CPS (rcsA, galF, wzi, and iscR), LPS (rfbABCD), type I and type III fimbriae (fim and mrk operon), cellulose (bcs operon), iron transporter (feoABC, fhuA, fhuF, tonB, exbB, and exbD), quorum sensing (lsr operon and sdiA), type II secretion system (T2SS) and type VI secretion system (T6SS) (tssG, hcp, and gspE). …”
Publicado 2023
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1146por Teixeira, Nathalia B., Rojas, Thais C. G., da Silveira, Wanderley D., Matheus-Guimarães, Cecília, Silva, Neusa P., Scaletsky, Isabel C. A.“…The EAF plasmid contains the bfp (bundle-forming pilus) operon and the perABC (plasmid encoded regulator) gene cluster. …”
Publicado 2015
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1147por Shi, Hao-wen, Wang, Li-ying, Li, Xin-xin, Liu, Xiao-meng, Hao, Tian-yi, He, Xiao-juan, Chen, San-feng“…CONCLUSIONS: The nif gene operon (nifBHDKENXhesAnifV) in Paenibacillus sp. WLY78 is significantly up-regulated in N(2)-fixing condition compared to non-N(2)-fixing condition. …”
Publicado 2016
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1148por Gopinath, Gopal R., Chase, Hannah R., Gangiredla, Jayanthi, Eshwar, Athmanya, Jang, Hyein, Patel, Isha, Negrete, Flavia, Finkelstein, Samantha, Park, Eunbi, Chung, TaeJung, Yoo, YeonJoo, Woo, JungHa, Lee, YouYoung, Park, Jihyeon, Choi, Hyerim, Jeong, Seungeun, Jun, Soyoung, Kim, Mijeong, Lee, Chaeyoon, Jeong, HyeJin, Fanning, Séamus, Stephan, Roger, Iversen, Carol, Reich, Felix, Klein, Günter, Lehner, Angelika, Tall, Ben D.“…The nine-gene, ~ 7.7 kbp malonate utilization operon was located in these strains between two conserved flanking genes, gyrB and katG. …”
Publicado 2018
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1149por van Mastrigt, Oscar, Di Stefano, Elisa, Hartono, Sylviani, Abee, Tjakko, Smid, Eddy J.“…These genes encode technological functions such as lactose utilisation (lacR-lacABCDFEGX), citrate uptake (citQRP), peptide degradation (pepO and pepE) and oligopeptide uptake (oppDFBCA), uptake of magnesium and manganese (2 mntH, corA), exopolysaccharides production (eps operon), bacteriophage resistance (1 hsdM, 1 hsdR and 7 different hsdS genes of a type I restriction-modification system, an operon of three genes encoding a putative type II restriction-modification system and an abortive infection gene) and stress resistance (2 uspA, cspC and cadCA). …”
Publicado 2018
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1150“…RESULTS: A two-fold decrease in expression of all genes of the cnt operon was observed by RT-qPCR in the rsp mutant compared to the parental strain, indicating that Rsp acts as an activator of the cnt operon. …”
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1151por Vahidinasab, Maliheh, Lilge, Lars, Reinfurt, Aline, Pfannstiel, Jens, Henkel, Marius, Morabbi Heravi, Kambiz, Hausmann, Rudolf“…B. subtilis synthesizes plipastatin by a non-ribosomal peptide synthetase encoded by the ppsABCDE operon. In this study, B. subtilis 3NA (a non-sporulation strain) was engineered to gain more insights about plipastatin mono-production. …”
Publicado 2020
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1152“…First, up to three copies of the crtMN operon from Staphylococcus aureus, encoding a yellow pigment, were incorporated at three ectopic sites within the B. subtilis chromosome, rendering engineered strains able to form yellow colonies. …”
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1153“…We additionally identified multiple transcriptional and translational layers of regulation of the rimM operon mRNA that occur in response to the depletion of functional tRNAs. …”
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1154por Lensmire, Joshua M., Wischer, Michael R., Kraemer-Zimpel, Cristina, Kies, Paige J., Sosinski, Lo, Ensink, Elliot, Dodson, Jack P., Shook, John C., Delekta, Phillip C., Cooper, Christopher C., Havlichek, Daniel H., Mulks, Martha H., Lunt, Sophia Y., Ravi, Janani, Hammer, Neal D.“…Based on these phenotypes, we name this transporter operon the glutathione import system (gisABCD). Ggt is encoded within the gisBCD operon, and we show that the enzyme is capable of liberating glutamate using either GSH or GSSG as substrates, demonstrating it is a bona fide γ–glutamyl transpeptidase. …”
Publicado 2023
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1155por McGregor, Alexandra K., Chan, Anson C.K., Schroeder, Megan D., Do, Long T.M., Saini, Gurpreet, Murphy, Michael E.P., Wolthers, Kirsten R.“…The last gene in the operon, hmuF encodes a member of the flavodoxin superfamily of proteins. …”
Publicado 2023
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1156“…RESULTS: Our phylogenetic trees revealed grouping based on operon structure, original homolog blasted from, and order of the homolog, suggesting a more ancestral origin of the multiple homolog phenomena. …”
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1157por Yan, Junxiang, Yang, Bin, Xue, Xinke, Li, Jinghao, Li, Yuehua, Li, Ang, Ding, Peng, Cao, Boyang“…The pyruvate dehydrogenase complex regulator (PdhR) was originally identified as a repressor of the pdhR-aceEF-lpd operon, which encodes the pyruvate dehydrogenase complex (PDHc) and PdhR itself. …”
Publicado 2023
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1158por Quiroz-Morales, Sara E., Muriel-Millán, Luis Felipe, Ponce-Soto, Gabriel Y., González-Valdez, Abigail, Castillo-Juárez, Israel, Servín-González, Luis, Soberón-Chávez, Gloria“…The Pqs QS system is composed of the PqsR transcriptional factor, which, coupled with the alkyl-quinolones HHQ or PQS, activates the transcription of the pqsABCDE operon. The products of the first four genes of this operon produce HHQ, which is then converted to PQS by PqsH, while PqsE forms a complex with RhlR and stabilizes it. …”
Publicado 2023
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1159“… : Coupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. …”
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1160Effect of iron on the expression of sirR and sitABC in biofilm-associated Staphylococcus epidermidispor Massonet, Caroline, Pintens, Valerie, Merckx, Rita, Anné, Jozef, Lammertyn, Elke, Van Eldere, Johan“…The expression of Fe-linked genes (sirR, sitABC operon) in Staphylococcus epidermidis, was compared in planktonic versus sessile bacteria in vitro and in vivo in a subcutaneous foreign body rat model. …”
Publicado 2006
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