“…We have successfully de novo assembled three complete chloroplast genomes from plant species with a range of nuclear genome sizes to demonstrate the universality of our approach: Solanum lycopersicum (0.9 Gb), Aegilops tauschii (4 Gb) and Paphiopedilum henryanum (25 Gb). We also highlight the need to optimize the choice of k and the amount of data used. …”
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“…Unlike many orchid plastomes (e.g., Paphiopedilum and Vanilla), the plastomes of Apostasioideae contain a full set of 11 functional NADH dehydrogenase (ndh) genes. …”
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“…For the other four orchid species, only one compatible fungus for each species was used, and the percentages of seedlings in epiphytic Dendrobium devonianum (67.2±2.9%) and D. nobile (38.9±2.8%) were much higher than those in terrestrial Paphiopedilum spicerianum (2.9±1.1%) and Arundina graminifolia (6.7±2.1%) at 90 days after sowing. …”
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“…High yielding viable protoplasts were isolated from the leaf base of Cymbidium (∼2.50 × 10(7)/g FW), Phalaenopsis (1.83 × 10(7)/g FW), Paphiopedilum (1.10 × 10(7)/g FW), Dendrobium (8.21 × 10(6)/g FW), Arundina (3.78 × 10(6)/g FW) orchids, and other economically important monocot crops including maize (Zea mays) (3.25 × 10(7)/g FW) and rice (Oryza sativa) (4.31 × 10(7)/g FW), which showed marked advantages over previous mesophyll protoplast isolation protocols. …”
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