-
241A cell-based ELISA as surrogate of virus neutralization assay for RBD SARS-CoV-2 specific antibodiespor Pi-Estopiñan, Franciscary, Pérez, María Teresa, Fraga, Anitza, Bergado, Gretchen, Díaz, Geidy D., Orosa, Ivette, Díaz, Marianniz, Solozábal, Joaquín Antonio, Rodríguez, Laura Marta, Garcia-Rivera, Dagmar, Macías, Consuelo, Jerez, Yanet, Casadesús, Ana V., Fernández-Marrero, Briandy, Bermúdez, Ernesto, Plasencia, Claudia A., Sánchez, Belinda, Hernández, Tays“…Herein, we present a safe cell-based ELISA-virus neutralization test (cbE-VNT) as a surrogate for the conventional viral neutralization assays that detects the inhibition of SARS-CoV-2 RBD binding to ACE2-bearing cells independently of species. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
242por Manuylov, Victor, Burgasova, Olga, Borisova, Olga, Smetanina, Svetlana, Vasina, Daria, Grigoriev, Igor, Kudryashova, Alexandra, Semashko, Maria, Cherepovich, Bogdan, Kharchenko, Olga, Kleymenov, Denis, Mazunina, Elena, Tkachuk, Artem, Gushchin, Vladimir“…The avidity index (AI) of IgG to the RBD of SARS-CoV-2 was determined for 71 patients with a mild (outpatient) course of COVID-19, including 39 primarily and 36 secondarily reinfected, and 92 patients with a severe (hospital) course of COVID-19, including 82 primarily and 10 secondarily infected. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
243“…Therefore, investigations into the RBD mutations that disrupt neutralization through convalescent antibodies are urgently required. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
244“…When considering emerging variations in Southeast Asia, 14 dominant mutations were analyzed by applying the structural and energetic characterization using MD simulations. The ones in the RBD region displayed higher affinity to ACE2 due to the improved interfacial stability of the RBD β-strand surrounding the ACE2 across salt bridge hotspots. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
245por Wagner, Angelika, Guzek, Angela, Ruff, Johanna, Jasinska, Joanna, Scheikl, Ute, Zwazl, Ines, Kundi, Michael, Stockinger, Hannes, Farcet, Maria R., Kreil, Thomas R., Hoeltl, Eva, Wiedermann, Ursula“…Of the 168 S1-reactive sera, 8.33% (n = 14) have detectable RBD-specific antibodies and 6.55% (n = 11) NCP-specific antibodies. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
246“…However, the interacting residues of RBD are found to be the same in Lamda (C.37) variant. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
247por Rodon, Jordi, Mykytyn, Anna Z., Cantero, Guillermo, Albulescu, Irina C., Bosch, Berend-Jan, Brix, Alexander, Audonnet, Jean-Christophe, Bensaid, Albert, Vergara-Alert, Júlia, Haagmans, Bart L., Segalés, Joaquim“…Our exploratory study supports further improvement of the RBD-based vaccine to prevent zoonotic spillover of MERS-CoV. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
248por Li, Tingting, Cai, Hongmin, Yao, Hebang, Zhou, Bingjie, Zhang, Ning, van Vlissingen, Martje Fentener, Kuiken, Thijs, Han, Wenyu, GeurtsvanKessel, Corine H., Gong, Yuhuan, Zhao, Yapei, Shen, Quan, Qin, Wenming, Tian, Xiao-Xu, Peng, Chao, Lai, Yanling, Wang, Yanxing, Hutter, Cedric A. J., Kuo, Shu-Ming, Bao, Juan, Liu, Caixuan, Wang, Yifan, Richard, Audrey S., Raoul, Hervé, Lan, Jiaming, Seeger, Markus A., Cong, Yao, Rockx, Barry, Wong, Gary, Bi, Yuhai, Lavillette, Dimitri, Li, DianfanEnlace del recurso
Publicado 2022
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
249por Choque-Guevara, Ricardo, Poma-Acevedo, Astrid, Montesinos-Millán, Ricardo, Rios-Matos, Dora, Gutiérrez-Manchay, Kristel, Montalvan-Avalos, Angela, Quiñones-Garcia, Stefany, Cauti-Mendoza, Maria de Grecia, Agurto-Arteaga, Andres, Ramirez-Ortiz, Ingrid, Criollo-Orozco, Manuel, Huaccachi-Gonzales, Edison, Romero, Yomara K., Perez-Martinez, Norma, Isasi-Rivas, Gisela, Sernaque-Aguilar, Yacory, Villanueva-Pérez, Doris, Ygnacio, Freddy, Vallejos-Sánchez, Katherine, Fernández-Sánchez, Manolo, Guevara-Sarmiento, Luis A., Fernández-Díaz, Manolo, Zimic, Mirko“…The RBD protein was expressed in insect cells and purified by chromatography until >95% purity. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
250por Cheng, Yi-Ling, Chao, Chiao-Hsuan, Lai, Yen-Chung, Hsieh, Kun-Han, Wang, Jen-Ren, Wan, Shu-Wen, Huang, Hong-Jyun, Chuang, Yung-Chun, Chuang, Woei-Jer, Yeh, Trai-Ming“…In addition, anti-S1-RBD IgG purified from S1-RBD hyperimmune rabbit sera could cross-react with both DENV envelope protein (E) and nonstructural protein 1 (NS1). …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
251por Dhanasooraj, Dhananjayan, Viswanathan, Prasanth, Saphia, Shammy, Jose, Beena Philomina, Parambath, Fairoz Cheriyalingal, Sivadas, Saritha, Akash, N. P., Vimisha, T. V., Nair, Priyanka Raveendranadhan, Mohan, Anuja, Hafeez, Nimin, Poovullathi, Jayesh Kumar, Vadekkandiyil, Shameer, Govindan, Sajeeth Kumar Keriyatt, Khobragade, Rajan, Aravindan, KP, Radhakrishnan, Chandni“…We analyzed the nucleotide sequence of the Receptor Binding Domain (RBD) region of SARS-CoV-2 by the Sanger sequencing using in-house developed methods. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
252“…Western blotting results show that nine mutants of the RBD protein had strong antigenic activity in vitro. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
253por Rahbar, Zahra, Nazarian, Shahram, Dorostkar, Ruhollah, Sotoodehnejadnematalahi, Fattah, Amani, Jafar“…CONCLUSION: Our findings showed that RBD is of great importance in vaccine design and it can be used to develop recombinant vaccines through induction of antibodies against RBD.…”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
254por Żeliszewska, Paulina, Wasilewska, Monika, Batys, Piotr, Pogoda, Katarzyna, Deptuła, Piotr, Bucki, Robert, Adamczyk, ZbigniewEnlace del recurso
Publicado 2022
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
255por Solis-Andrade, Karla I., Gonzalez-Ortega, Omar, Govea-Alonso, Dania O., Comas-Garcia, Mauricio, Rosales-Mendoza, Sergio“…Herein a recombinant chimeric antigen called LTB-RBD is described, which comprises the B subunit of the heat-labile enterotoxin from E. coli and a segment of the RBD from SARS-CoV-2 (aa 439-504, carrying B and T cell epitopes) from the Wuhan sequence and the variant of concern (VOC)—delta. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
256por Patel, DR, Minns, AM, Sim, DG, Field, CJ, Kerr, AE, Heinly, T, Luley, EH, Rossi, R.M., Bator, C., Moustafa, I.M., Hafenstein, SL, Lindner, SE, Sutton, TC“…Using this RBD-grafted SpyCage scaffold (RBD+SpyCage), we performed two unadjuvanted intranasal vaccination studies in the “gold-standard” preclinical Syrian hamster model. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
257por Rodríguez, María Celeste, Ceaglio, Natalia, Gugliotta, Agustina, Villarraza, Javier, Garay, Ernesto, Fuselli, Antonela, Gastaldi, Victoria, Tardivo, María Belén, Antuña, Sebastián, Fontana, Diego, Prieto, Claudio“…In this study, we report the production and purification process of the receptor-binding domain (RBD) of SARS-CoV-2 in HEK293 cells, which allowed the design, optimization, and validation of an indirect ELISA (iELISA) for the detection of human anti-RBD antibodies. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
258por Liu, Zheng, Yang, Chenglu, Zhang, Haokun, Cao, Guojie, Wang, Senzhen, Yin, Siwen, Wang, Yanming“…Here, we showed that dimeric S(RBD)-Fc and tetrameric 2xS(RBD)-Fc fusion proteins bind ACE2 with different affinity and block SARS-CoV-2 pseudoviral infection. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
259por Buonocore, Michela, Santoro, Angelo, Grimaldi, Manuela, Covelli, Verdiana, Firoznezhad, Mohammad, Rodriquez, Manuela, Santin, Matteo, D'Ursi, Anna Maria“…In this work, we present the development of a simplified system that would afford to study the change in the SARS-CoV-2 S RBD/ACE2 binding related to the frequent mutations. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
260por Timofeeva, Anna M., Sedykh, Sergey E., Ermakov, Evgeny A., Matveev, Andrey L., Odegova, Eva I., Sedykh, Tatiana A., Shcherbakov, Dmitry N., Merkuleva, Iuliia A., Volosnikova, Ekaterina A., Nesmeyanova, Valentina S., Tikunova, Nina V., Nevinsky, Georgy A.Enlace del recurso
Publicado 2022
Enlace del recurso
Enlace del recurso
Online Artículo Texto