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3421por Kazemi, Soheila, López-Muñoz, Alberto Domingo, Hollý, Jaroslav, Jin, Ling, Yewdell, Jonathan W., Dolan, Brian P.“…When ranked for either viral infectivity or RBD binding, mouse ACE2 had a weak to undetectable affinity for S-protein while human ACE2 was the highest level detected and feline ACE2 had an intermediate phenotype. …”
Publicado 2021
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3422“…CO(2) is a potent inhibitor of cellular proinflammatory responses caused by H(2)O(2) or the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2. ERK1/2 activated by the combined action of RBD and cytokines crucial for the development of severe COVID-19, i.e. interferon-gamma (IFNγ) and tumour necrosis factor-α (TNFα), are more effectively inactivated by CO(2) than by dexamethasone or acetylsalicylic acid in human bronchial epithelial cells. …”
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3423por Lin, Hui-Tsu, Chen, Cheng-Cheung, Chiao, Der-Jiang, Chang, Tein-Yao, Chen, Xin-An, Young, Jenn-Jong, Kuo, Szu-Cheng“…The S1 domain of S protein, which contains a critical receptor-binding domain (RBD) antigen, potentially induces protective immunoreactivities against SARS-CoV-2. …”
Publicado 2021
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3424por Dashdorj, Naranjargal J., Wirz, Oliver F., Röltgen, Katharina, Haraguchi, Emily, Buzzanco, Anthony S., Sibai, Mamdouh, Wang, Hannah, Miller, Jacob A., Solis, Daniel, Sahoo, Malaya K., Arunachalam, Prabhu S., Lee, Alexandra S., Shah, Mihir M., Liu, James, Byambabaatar, Sumiya, Bat-Ulzii, Purevjargal, Enkhbat, Anir, Batbold, Enkhtuul, Zulkhuu, Delgersaikhan, Ochirsum, Byambasuren, Khurelsukh, Tungalag, Dalantai, Ganbold, Burged, Natsagdorj, Baatarsuren, Uurtsaikh, Ariungerel, Nomin, Oidovsambuu, Odgerel, Bungert, Andreas S., Genden, Zulkhuu, Yagaanbuyant, Dahgwahdorj, Mordorj, Altankhuu, Pulendran, Bali, Chinthrajah, Sharon, Nadeau, Kari C., Jardetzky, Theodore, Wilbur, James L., Wohlstadter, Jacob N., Sigal, George B., Pinsky, Benjamin A., Boyd, Scott D., Dashdorj, Naranbaatar D.“…Functional antibody testing with a panel of nine SARS-CoV-2 viral variant receptor binding domain (RBD) proteins revealed marked differences in vaccine responses, with low antibody levels and RBD-ACE2 blocking activity stimulated by the Sinopharm and Sputnik V vaccines in comparison to the AstraZeneca or Pfizer/BioNTech vaccines. …”
Publicado 2021
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3425por da Silva, Laís Teodoro, Ortega, Marina Mazzilli, Tiyo, Bruna Tiaki, Viana, Isabelle Freire Tabosa, de Lima, Tayná Evily, Tozetto-Mendoza, Tania Regina, Oliveira, Luanda Mara da Silva, Teixeira, Franciane Mouradian Emidio, Lins, Roberto Dias, de Almeida, Alexandre, Mendes-Correa, Maria Cassia, da Silva Duarte, Alberto Jose, Oshiro, Telma Miyuki“…Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. …”
Publicado 2021
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3426por Liu, Binjian, Su, Xibin, Yu, George, Yang, Shuling, Wang, Feng, Huang, Tao, Zhou, Liuyong, Hui, Zhiqiang, Liao, Yixian, Qiu, Yuan, Huang, Juan, Gao, Hong, Liu, Justin, Zhong, Yaoqi“…METHODS: The SARS-CoV-2 receptor-binding domain (RBD) and a stabilized version of the spike ectodomain as antigens were detected by CLIA. …”
Publicado 2022
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3427por Zinatizadeh, Mohammad Reza, Zarandi, Peyman Kheirandish, Zinatizadeh, Maryam, Yousefi, Mohammad Hadi, Amani, Jaffar, Rezaei, Nima“…Mutations in the receptor-binding domain (RBD) and n-terminal domain (NTD) have been found throughout all five identified VoCs. …”
Publicado 2022
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3428por Abidi, Syed Hani, Imtiaz, Kehkashan, Kanji, Akbar, Qaiser, Shama, Khan, Erum, Iqbal, Kiran, Veldhoen, Marc, Ghias, Kulsoom, Simas, J. Pedro, Hasan, Zahra“…For the micro‐neutralization assay, 19 serum samples, with positive IgG titers against Spike Receptor-Binding Domain (RBD) were tested. After 24 hours, infected cells were inspected for the presence of a cytopathic effect, lysed and RNA RT-PCR conducted for SARS-CoV-2. …”
Publicado 2021
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3429por Zhou, Zhou, Zhou, Xiaoting, Zhou, Xiaoxia, Xiang, Yaqin, Zhu, Liping, Qin, Lixia, Wang, Yige, Pan, Hongxu, Zhao, Yuwen, Sun, Qiying, Xu, Qian, Wu, Xinyin, Yan, Xinxiang, Guo, Jifeng, Tang, Beisha, Liu, Zhenhua“…With logistic regression analysis for potentially related factors, age, UPDRS total score, RBD, hyposmia, depression, and fatigue may be associated with PD with AutD. …”
Publicado 2021
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3430por Ubah, Obinna C., Lake, Eric W., Gunaratne, Gihan S., Gallant, Joseph P., Fernie, Marie, Robertson, Austin J., Marchant, Jonathan S., Bold, Tyler D., Langlois, Ryan A., Matchett, William E., Thiede, Joshua M., Shi, Ke, Yin, Lulu, Moeller, Nicholas H., Banerjee, Surajit, Ferguson, Laura, Kovaleva, Marina, Porter, Andrew J., Aihara, Hideki, LeBeau, Aaron M., Barelle, Caroline J.“…Crystallographic analysis of two VNARs found that they recognized separate epitopes on the RBD and had distinctly different mechanisms of virus neutralization unique to VNARs. …”
Publicado 2021
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3431por Vanshylla, Kanika, Fan, Chengcheng, Wunsch, Marie, Poopalasingam, Nareshkumar, Meijers, Matthijs, Kreer, Christoph, Kleipass, Franziska, Ruchnewitz, Denis, Ercanoglu, Meryem S., Gruell, Henning, Münn, Friederike, Pohl, Kai, Janicki, Hanna, Nolden, Tobias, Bartl, Simone, Stein, Saskia C., Augustin, Max, Dewald, Felix, Gieselmann, Lutz, Schommers, Philipp, Schulz, Thomas F., Sander, Leif Erik, Koch, Manuel, Łuksza, Marta, Lässig, Michael, Bjorkman, Pamela J., Klein, Florian“…One of these broadly neutralizing potent antibodies, R40-1G8, is a IGHV3-53 RBD-class-1 antibody. Remarkably, cryo-EM analysis revealed that R40-1G8 has a flexible binding mode, targeting both “up” and “down” conformations of the RBD. …”
Publicado 2022
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3432por Khan, Abbas, Hussain, Sarfaraz, Ahmad, Sajjad, Suleman, Muhammad, Bukhari, Imrana, Khan, Taimoor, Rashid, Farooq, Azad, Abul Kalam, Waseem, Muhammad, Khan, Wajid, Hussain, Zahid, Khan, Asghar, Ali, Syed Shujait, Qin, Qiyao, Wei, Dong-Qing“…We analyzed the binding pattern of wild type and mutants (Spike RBD) to the ACE2 receptor by deciphering variations in the amino acids’ interaction networks by graph kernels along with evolutionary, network metrics, and energetic information. …”
Publicado 2022
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3433por Chen, Xiahui, Kang, Shoukai, Ikbal, Md Ashif, Zhao, Zhi, Pan, Yuxin, Zuo, Jiawei, Gu, Liangcai, Wang, Chao“…With Ebola virus secreted glycoprotein (sGP) and a SARS-CoV-2 spike protein receptor binding domain (RBD) as targets, Nano2RED showed a high sensitivity (the limit of detection of ∼10 pg /mL, or 0.13 pM for sGP and ∼40 pg/mL, or ∼1.3 pM for RBD in diluted human serum), a high specificity, a large dynamic range (∼7 logs),and fast readout within minutes. …”
Publicado 2022
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3434por Sartor, Ivaine Tais Sauthier, Varela, Fernanda Hammes, Meireles, Mariana Rost, Kern, Luciane Beatriz, Azevedo, Thaís Raupp, Giannini, Gabriela Luchiari Tumioto, da Silva, Mariana Soares, Demoliner, Meriane, Gularte, Juliana Schons, de Almeida, Paula Rodrigues, Fleck, Juliane Deise, Zavaglia, Gabriela Oliveira, Fernandes, Ingrid Rodrigues, de David, Caroline Nespolo, Santos, Amanda Paz, de Almeida, Walquiria Aparecida Ferreira, Porto, Victor Bertollo Gomes, Scotta, Marcelo Comerlato, Vieira, Gustavo Fioravanti, Spilki, Fernando Rosado, Stein, Renato T., Polese-Bonatto, Márcia“…We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October in Porto Alegre, Brazil. …”
Publicado 2022
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3435por Xu, Shiqi, Wang, Yifan, Wang, Yanxing, Zhang, Chao, Hong, Qin, Gu, Chenjian, Xu, Rong, Wang, Tingfeng, Yang, Yong, Zang, Jinkai, Zhou, Yu, Li, Zuyang, Liu, Qixing, Zhou, Bingjie, Bai, Lulu, Zhu, Yuanfei, Deng, Qiang, Wang, Haikun, Lavillette, Dimitri, Wong, Gary, Xie, Youhua, Cong, Yao, Huang, Zhong“…S5G2 targets the highly conserved RBD core region and exhibits comparable neutralization towards the variant panel. …”
Publicado 2022
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3436An encodable multiplex microsphere-phase amplification sensing platform detects SARS-CoV-2 mutationspor Zhong, Zecheng, Wang, Jin, He, Shuizhen, Su, Xiaosong, Huang, Weida, Chen, Mengyuan, Zhuo, Zhihao, Zhu, Xiaomei, Fang, Mujin, Li, Tingdong, Zhang, Shiyin, Ge, Shengxiang, Zhang, Jun, Xia, Ningshao“…SARS-CoV-2 variants of concern (VOCs) contain several single-nucleotide variants (SNVs) at key sites in the receptor-binding region (RBD) that enhance infectivity and transmission, as well as cause immune escape, resulting in an aggravation of the coronavirus disease 2019 (COVID-19) pandemic. …”
Publicado 2022
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3437por Jayathilaka, Deshni, Jeewandara, Chandima, Gomes, Laksiri, Jayadas, Tibutius Thanesh Pramanayagam, Kamaladasa, Achala, Somathilake, Gayasha, Guruge, Dinuka, Pushpakumara, Pradeep Darshana, Ranasinghe, Thushali, Aberathna, Inoka Sepali, Danasekara, Saubhagya, Gunathilaka, Buddini, Kuruppu, Heshan, Wijewickrama, Ananda, Wijayamuni, Ruwan, Schimanski, Lisa, Tan, T K, Ogg, Graham S, Townsend, Alain, Malavige, Gathsaurie Neelika“…The IgG and IgA responses were assessed to S1, S2, RBD, and N proteins in patients at 4 weeks and 12 weeks since the onset of illness or following vaccination. …”
Publicado 2022
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3438por Tsuda, Masumi, Horio, Runa, Wang, Lei, Takenami, Tomoko, Moriya, Jun, Suzuka, Jun, Sugino, Hirokazu, Tanei, Zenichi, Tanino, Mishie, Tanaka, Shinya“…Here, we established a novel detection technique for activated Rac/Cdc42 in human colon cancer FFPE tissues by using a p21-activated kinase (PAK)-Rac binding domain (RBD) detection probe fused with glutathione S-transferase (GST), designated GST-PAK-RBD, and novel rapid-immunohistochemistry (R-IHC) systems using noncontact alterating-current electric field mixing, although there is a technical limitation in that it may not distinguish between Rac members and Cdc42. …”
Publicado 2022
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3439por Bonhomme, Marie E., Bonhomme, Cyrille J., Strelow, Lisa, Chaudhari, Atul, Howlett, Adrienne, Breidenbach, Carl, Hester, Jack, Hammond, Christopher, Fuzy, Michéal, Harvey, Laura, Swanner, Vanessa, Ellis, Jeymie, Greway, Rebecca R., Pisciella, Victoria A., Green, Tina, Kierstead, Lisa“…An evaluation to compare the neutralizing activity in the MNT assay to the IgG measured using the MSD ECL assay showed a strong correlation between the presence of neutralizing activity and amount of antibodies against the spike and RBD proteins in sera from both convalescent and vaccinated individuals. …”
Publicado 2022
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3440por He, Wan-ting, Musharrafieh, Rami, Song, Ge, Dueker, Katharina, Tse, Longping V., Martinez, David R., Schäfer, Alexandra, Callaghan, Sean, Yong, Peter, Beutler, Nathan, Torres, Jonathan L., Volk, Reid M., Zhou, Panpan, Yuan, Meng, Liu, Hejun, Anzanello, Fabio, Capozzola, Tazio, Parren, Mara, Garcia, Elijah, Rawlings, Stephen A., Smith, Davey M., Wilson, Ian A., Safonova, Yana, Ward, Andrew B., Rogers, Thomas F., Baric, Ralph S., Gralinski, Lisa E., Burton, Dennis R., Andrabi, Raiees“…Consistent with targeting of conserved sites, select RBD bnAbs exhibited in vivo protective efficacy against diverse SARS-like coronaviruses in a prophylaxis challenge model. …”
Publicado 2022
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