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3841por Puthanakit, Thanyawee, Nantanee, Rapisa, Jaru-Ampornpan, Peera, Chantasrisawad, Napaporn, Sophonphan, Jiratchaya, Meepuksom, Thutsanun, Jupimai, Thidarat, Sodsai, Pimpayao, Anugulruengkitt, Suvaporn, Hirankarn, Nattiya“…At day 14, the geometric mean(GM) of anti-S-RBD IgG in SV3w/PZ30µg was 4713 (95 %CI 4127–5382) binding-antibody unit (BAU)/ml, while geometric mean ratio(GMR) was 1.28 (1.09–1.51) in SV6w/PZ30µg. …”
Publicado 2022
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3842por Karachaliou, Marianna, Moncunill, Gemma, Espinosa, Ana, Castaño-Vinyals, Gemma, Rubio, Rocío, Vidal, Marta, Jiménez, Alfons, Prados, Esther, Carreras, Anna, Cortés, Beatriz, Blay, Natàlia, Bañuls, Marc, Pleguezuelos, Vanessa, Melero, Natalia Rodrigo, Serra, Pau, Parras, Daniel, Izquierdo, Luis, Santamaría, Pere, Carolis, Carlo, Papantoniou, Kyriaki, Goldberg, Ximena, Aguilar, Ruth, Garcia-Aymerich, Judith, de Cid, Rafael, Kogevinas, Manolis, Dobaño, Carlota“…Moreover, the analysis on kinetics revealed that among all responses, IgG RBD, IgA RBD, and IgG S2 decreased less within 1 year after infection. …”
Publicado 2022
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3843por Toledo-Romaní, María Eugenia, García-Carmenate, Mayra, Valenzuela-Silva, Carmen, Baldoquín-Rodríguez, Waldemar, Martínez-Pérez, Marisel, Rodríguez-González, Meiby, Paredes-Moreno, Beatriz, Mendoza-Hernández, Ivis, González-Mujica Romero, Raúl, Samón-Tabio, Oscar, Velazco-Villares, Pablo, Bacallao-Castillo, Juan Pablo, Licea-Martín, Ernesto, Rodríguez-Ortega, Misladys, Herrera-Marrero, Nuris, Caballero-González, Esperanza, Egües-Torres, Liudmila, Duartes-González, Reinaldo, García-Blanco, Serguey, Pérez-Cabrera, Suzette, Huete-Ferreira, Santos, Idalmis-Cisnero, Kirenia, Fonte-Galindo, Omayda, Meliá-Pérez, Dania, Rojas-Remedios, Ivonne, Doroud, Delaram, Gouya, Mohammad Mehdi, Biglari, Alireza, Fernández-Castillo, Sonsire, Climent-Ruiz, Yanet, Valdes-Balbín, Yury, García-Rivera, Dagmar, Van der Stuyft, Patrick, Verez-Bencomo, Vicente“…A third heterologous dose of SOBERANA-Plus (RBD-dimer) further increased neutralising antibodies. …”
Publicado 2022
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3844
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3845por D’Alessio, Federico, Lione, Lucia, Salvatori, Erika, Bucci, Federica, Muzi, Alessia, Roscilli, Giuseppe, Compagnone, Mirco, Pinto, Eleonora, Battistuzzi, Gianfranco, Conforti, Antonella, Aurisicchio, Luigi, Palombo, Fabio“…As a model, we used COVID-eVax, a plasmid DNA-based vaccine that targets the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. Increased nicked DNA was produced by using either an accelerated stability protocol or a lyophilization protocol. …”
Publicado 2023
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3846“…In this study, we used human cultured mast cells that are plentiful in the lungs and showed that recombinant SARS-CoV-2 full-length S protein (1–10 ng/mL), but not its receptor-binding domain (RBD), stimulates the secretion of the proinflammatory cytokine interleukin-1β (IL-1β) as well as the proteolytic enzymes chymase and tryptase. …”
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3847“…The BA.3 S protein displays increased heterogeneity, with the majority in the all “up” RBD state. The different conformations preferences of the S protein are consistent with their varied transmissibility. …”
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3848por Mulgaonkar, Nirmitee, Wang, Haoqi, Zhang, Junrui, Roundy, Christopher M., Tang, Wendy, Chaki, Sankar Prasad, Pauvolid-Corrêa, Alex, Hamer, Gabriel L., Fernando, Sandun“…We employed computational methodologies, including molecular mechanics and machine learning tools, to identify drugs that could potentially disrupt the SARS-CoV-2 spike RBD-ACE2 protein interaction. This led to the identification of two FDA-approved small molecule drugs, M and T, conventionally used for treating asthma and hypertension, respectively. …”
Publicado 2023
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3849por Martin, Cole L., Chester, David W., Radka, Christopher D., Pan, Lurong, Yang, Zhengrong, Hart, Rachel C., Binshtein, Elad M., Wang, Zhao, Nagy, Lisa, DeLucas, Lawrence J., Aller, Stephen G.“…Contrary to a previous report, XptA2 is pentameric. RBD-B exhibits an indentation from crystal packing that indicates loose association with the shell and a hotspot for possible receptor binding or a trigger for conformational dynamics. …”
Publicado 2023
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3850“…Therefore, the key site mutation in the S gene is of high interest. Among them, RBD, NTD, and furin cleavage sites are the most mutable regions with the most mutation sites and the most serious consequences for SARS-CoV-2 biological characteristics, including infectivity, pathogenicity, natural immunity, vaccine efficacy, and antibody therapeutics. …”
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3851por Lee, Young-Tae, Sickmier, E. Allen, Grigoriu, Simina, Castro, Jennifer, Boriack-Sjodin, P. Ann“…Due to differences in the bound substrates and global domain orientations, the localized loop conformations and occupancy of dsRNA-binding domain 2 (dsRBD2) differ between the mammalian DHX9 and MLE structures. …”
Publicado 2023
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3852por Edinger, Nufar, Lebendiker, Mario, Klein, Shoshana, Zigler, Maya, Langut, Yael, Levitzki, Alexander“…In this study, we describe a novel recombinant chimeric protein, dsRBEC, which can bind polyIC and deliver it selectively into EGFR over-expressing tumor cells. dsRBEC, comprises the dsRNA binding domain (dsRBD) of human PKR (hPKR), which serves as the polyIC binding moiety, fused to human EGF (hEGF), the targeting moiety. dsRBEC shows high affinity towards EGFR and triggers ligand-induced endocytosis of the receptor, thus leading to the selective internalization of polyIC into EGFR over-expressing tumor cells. …”
Publicado 2016
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3853“…Co-expression of N-terminal and RNA binding domain (RBD) deletion mutants of Nucleolin or inhibiting 45S rRNA synthesis reduces the sequestration of H2B-ECFP in the nucleolus. …”
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3854“…To obtain an aptamer with a high affinity for vascular endothelial growth factor (VEGF), we focused on the receptor-binding domain (RBD) of VEGF as a target epitope. Three rounds of screening gave Vap7, which bound to the VEGF isoforms VEGF(121) and VEGF(165) with K(D) values of 1.0 nM and 20 nM, respectively. …”
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3855“…In the first experiment, 10 local Turkish bottle gourd genotypes and two commercial watermelon cultivars were screened under 2 N doses (0.3 mM and 3.0 mM N) in RBD design with three replications for six weeks. …”
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3856por Wrapp, Daniel, Wang, Nianshuang, Corbett, Kizzmekia S., Goldsmith, Jory A., Hsieh, Ching-Lin, Abiona, Olubukola, Graham, Barney S., McLellan, Jason S.“…Additionally we tested several published SARS-CoV RBD-specific monoclonal antibodies and found that they do not have appreciable binding to nCoV-2019 S, suggesting antibody cross-reactivity may be limited between the two virus RBDs. …”
Publicado 2020
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3857por Henderson, Rory, Edwards, Robert J, Mansouri, Katayoun, Janowska, Katarzyna, Stalls, Victoria, Gobeil, Sophie, Kopp, Megan, Hsu, Allen, Borgnia, Mario, Parks, Rob, Haynes, Barton F., Acharya, Priyamvada“…Based on this analysis, we developed two soluble ectodomain constructs in which the highly immunogenic and mobile receptor binding domain (RBD) is locked in either the all-RBDs ‘down’ position or is induced to display a previously unobserved in SARS-CoV-2 2-RBDs ‘up’ configuration. …”
Publicado 2020
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3858por Yuan, Meng, Liu, Hejun, Wu, Nicholas C., Lee, Chang-Chun D., Zhu, Xueyong, Zhao, Fangzhu, Huang, Deli, Yu, Wenli, Hua, Yuanzi, Tien, Henry, Rogers, Thomas F., Landais, Elise, Sok, Devin, Jardine, Joseph G., Burton, Dennis R., Wilson, Ian A.“…We analyzed 294 SARS-CoV-2 antibodies and found that IGHV3–53 is the most frequently used IGHV gene for targeting the receptor binding domain (RBD) of the spike (S) protein. We determined crystal structures of two IGHV3–53 neutralizing antibodies +/− Fab CR3022 ranging from 2.33 to 3.11 Å resolution. …”
Publicado 2020
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3859“…In addition, the enhancement was mediated by ACE2 binding of RBD domain of SARS-CoV-2 spike. These results highlighted the altered activity of ACE2 during SARS-CoV-2 infection and would shed new lights on the pathogenesis of COVID-19 and its complications for better treatments.…”
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3860por Bouricha, El Mehdi, Hakmi, Mohammed, Akachar, Jihane, Belyamani, Lahcen, Ibrahimi, Azeddine“…Our results showed that all studied animals are potentially susceptible to SARS-CoV-2 infection with a slight difference in the binding affinity and stability of their ACE2–RBD complexes. Furthermore, we identified a specific substitution of tyrosine to histidine at position 41 in ACE2 that likely reduces the affinity to SARS-CoV-2 in horses and greater horseshoe bats. …”
Publicado 2020
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