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4161por Guzmán-Bringas, Omar U., Gómez-Castellano, Keyla M., González-González, Edith, Salinas-Trujano, Juana, Vázquez-Leyva, Said, Vallejo-Castillo, Luis, Pérez-Tapia, Sonia M., Almagro, Juan C.“…Three out of 24 specific clones for the RBD isolated from the libraries, with affinity in the low nanomolar range and sub-optimal in vitro neutralization in PRNT, were affinity optimized via a method called “Rapid Affinity Maturation” (RAM). …”
Publicado 2023
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4162“…Leveraging recent advances in computational modeling of proteins with AlphaFold2 (AF2) we provide a complete curated data set of all single mutations from each of the 7 main SARS-CoV-2 lineages spike protein receptor binding domain (RBD) resulting in 3819X7 = 26733 PDB structures. …”
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4163por Oktay, Esra, Alem, Farhang, Hernandez, Keziah, Girgis, Michael, Green, Christopher, Mathur, Divita, Medintz, Igor L., Narayanan, Aarthi, Veneziano, Remi“…Here, we are leveraging the unique properties of these DNA-NPs and demonstrate that precisely patterning ten copies of a reconstituted trimer of the receptor binding domain (RBD) of SARS-CoV-2 along with CpG adjuvants on the DNA-NPs is able to elicit a robust protective immunity against SARS-CoV-2 in a mouse model. …”
Publicado 2023
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4164por García-Vega, Melissa, Melgoza-González, Edgar A., Hernández-Valenzuela, Sofía, Hinojosa-Trujillo, Diana, Reséndiz-Sandoval, Mónica, Llamas-Covarrubias, Mara Anais, Loza-López, Martín, Valenzuela, Olivia, Soto-Gaxiola, Alan, Hernández-Oñate, Miguel A., Mata-Haro, Verónica, Cassaniti, Irene, Sammartino, Josè Camilla, Ferrari, Alessandro, Simonelli, Luca, Pedotti, Mattia, Sun, Rui, Zuo, Fanglei, Baldanti, Fausto, Varani, Luca, Marcotte, Harold, Pan-Hammarström, Qiang, Hernández, Jesús“…Biophysical and structural analysis showed that 19n01 cross-competes with ACE2 binding to the receptor-binding domain (RBD) and the kinetic parameters confirmed the high affinity against the Omicron sublineages (K(D) of 61 and 30 nM for BA.2 and BA.4/5, respectively). …”
Publicado 2023
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4165por Chi, Xiangyang, Xia, Lingyun, Zhang, Guanying, Chi, Ximin, Huang, Bangdong, Zhang, Yuanyuan, Chen, Zhengshan, Han, Jin, Wu, Liushu, Li, Zeya, Sun, Hancong, Huang, Ping, Yu, Changming, Chen, Wei, Zhou, Qiang“…We found that the epitopes of the H-RBD class antibodies were significantly less affected by Omicron mutations than other classes. …”
Publicado 2023
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4166por Kwon, Paul S., Xu, Shirley, Oh, Hanseul, Kwon, Seok-Joon, Rodrigues, Andre L., Feroz, Maisha, Fraser, Keith, He, Peng, Zhang, Fuming, Hong, Jung Joo, Linhardt, Robert J., Dordick, Jonathan S.“…Specifically, heparan sulfate and suramin had enhanced preferential binding for Omicron RBD, and suramin is most potent against the live SARS-CoV-2 Omicron variant (B.1.1.529) when compared to wild type and Delta (B.1.617.2) variants in vitro. …”
Publicado 2023
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4167por Sun, Lifeng, Chopra, Pradeep, Tomris, Ilhan, van der Woude, Roosmarijn, Liu, Lin, de Vries, Robert P., Boons, Geert-Jan“…The heparin mimetics could inhibit the binding of the SARS-CoV-2 spike or RBD to immobilized heparin or to Vero E6 cells. …”
Publicado 2023
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4168por Karimi, Sahar, Nazarian, Shahram, Sotoodehnejadnematalahi, Fattah, Dorostkar, Roohollah, Amani, Jafar“…Evaluation the immunogenicity of a recombinant fragment of the spike protein (rfsp) that is comprised of Receptor Binding Domain (RBD), S1/S2 cleavage site, and fusion peptide (FP) as immunogenic proteins of SARS-COV-2, in BALB/c mice and evaluation of the efficacy of epitopes rfsp as a multi-subunit chimeric vaccine. …”
Publicado 2023
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4169por Giai, Constanza, Salassa, Betiana Nebaí, Zarelli, Valeria Eugenia, Bello, Oscar Daniel, Vanrell, María Cristina, Ojeda, Diego Sebastian, Gamarnik, Andrea, Colombo, María Isabel“…Our results indicate that at 21–25 days after the administration of the first doses of Sputnik V the large majority of the people vaccinated 80% (n = 15) presented high humoral responses as determined by the measurement of IgG against the Spike protein and the Receptor Binding Domain (RBD). In the case of those vaccinated with AstraZeneca, the percentage was 80% (n = 15) whereas this value was reduced to only 25% (n = 16) in persons that received Sinopharm. …”
Publicado 2023
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4170por Rabdano, Sevastyan O., Ruzanova, Ellina A., Pletyukhina, Iuliia V., Saveliev, Nikita S., Kryshen, Kirill L., Katelnikova, Anastasiia E., Beltyukov, Petr P., Fakhretdinova, Liliya N., Safi, Ariana S., Rudakov, German O., Arakelov, Sergei A., Andreev, Igor V., Kofiadi, Ilya A., Khaitov, Musa R., Valenta, Rudolf, Kryuchko, Daria S., Berzin, Igor A., Belozerova, Natalia S., Evtushenko, Anatoly E., Truhin, Viktor P., Skvortsova, Veronika I.“…The vast majority of SARS-CoV-2 vaccines which are licensed or under development focus on the spike (S) protein and its receptor binding domain (RBD). However, the S protein shows considerable sequence variations among variants of concern. …”
Publicado 2023
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4171por Lenguiya, Léadisaelle Hosanna, Fritz, Matthieu, De Fonclare, Daphné de Riols, Corbet, Sandrine, Becquart, Pierre, Mbou, Christophe, Nguie, Ruben Junias, Mouellet, Wivine Salva, Demboux, Jordy Exaucé Lyelet, N’kaya-Tobi, Issamou Mayengue, Pembe, Koukouikila-Koussounda, Félix, Ar Gouilh, Meriadeg, Leroy, Eric M., Niama, Fabien Roch“…Real-time PCR and the Luminex platform were used to detect SARS-CoV-2 RNA and antibodies to SARS-CoV-2 RBD and S proteins, respectively. Our results show for the first time the simultaneous circulation of several variants of SARS-CoV-2, including viruses from clades 20A and 20H and a putative recombinant variant between viruses from clades 20B and 20H. …”
Publicado 2023
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4172por Trzaskowski, Maciej, Mazurkiewicz-Pisarek, Anna, Trzciński, Jakub Waldemar, Drozd, Marcin, Podgórski, Rafał, Zabost, Anna, Augustynowicz-Kopeć, Ewa“…The receptor-binding domain (RBD) of spike protein from SARS-CoV-2 was selected as a binding molecule for the study. …”
Publicado 2023
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4173por Wouters, Elise, Verbrugghe, Caro, Abdelnabi, Rana, Devloo, Rosalie, De Clippel, Dorien, Jochmans, Dirk, De Bleser, Dominique, Weynand, Birgit, Compernolle, Veerle, Neyts, Johan, Feys, Hendrik B.“…METHODS: We tested if CCP is an effective prophylactic against SARS-CoV-2 infection by the intranasal administration of 25 μL CCP/nostril (i.e. 0.01–0.06 mg anti-RBD antibodies/kg) in hamsters exposed to infected littermates. …”
Publicado 2023
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4174por Refaat Fahim, John, Darwish, Ahmed G., El Zawily, Amr, Wells, Jacob, Abourehab, Mohammed A.S., Yehia Desoukey, Samar, Zekry Attia, Eman“…Furthermore, molecular docking and dynamics analyses of both the major and the key VOCs displayed their moderate to promising binding interactions with four main proteins of SARS-CoV-2, including Mpro, PLpro, RdRp, and spike S1 subunit RBD. The current results collectively cast new light on the chemical diversity of the VOCs of Chorisia plants as well as their chemotaxonomic and biological relevance.…”
Publicado 2023
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4175por Syrimi, Natalia, Sourri, Flora, Giannakopoulou, Maria-Christina, Karamanis, Dimitrios, Pantousas, Asterios, Georgota, Persefoni, Rokka, Eleni, Vladeni, Zoe, Tsiantoula, Euaggelia, Soukara, Evangelia, Lavda, Nikoletta, Gkaragkanis, Dimitrios, Zisaki, Aikaterini, Vakalidis, Panagiotis, Goula, Vasiliki, Loupou, Evdokia, Palaiodimos, Leonidas, Hatzigeorgiou, Dimitrios“…An anti-S1 receptor binding domain (RBD) antibody assay was used to assess humoral response, and cellular immunity was estimated with an INF-γ release assay (IGRA). …”
Publicado 2023
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4176por Izadi, Shiva, Vavra, Ulrike, Melnik, Stanislav, Grünwald-Gruber, Clemens, Föderl-Höbenreich, Esther, Sack, Markus, Zatloukal, Kurt, Glössl, Josef, Stöger, Eva, Mach, Lukas, Castilho, Alexandra, Strasser, Richard“…The in vivo deglycosylated ACE2-Fc carrying single GlcNAc residues displayed increased affinity to the receptor-binding domain (RBD) of SARS-CoV-2 as well as improved virus neutralization activity and thus is a promising drug candidate to block coronavirus infection.…”
Publicado 2023
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4177por Rungrojcharoenkit, Kamonthip, Suthangkornkul, Rungarun, Utennam, Darunee, Buddhari, Darunee, Pinpaiboon, Soontorn, Mongkolsirichaikul, Duangrat, Fernandez, Stefan, Jones, Anthony R., Cotrone, Thomas S., Hunsawong, Taweewun“…Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. …”
Publicado 2023
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4178por Wang, Ke, Pan, Yu, Wang, Dianbing, Yuan, Ye, Li, Min, Chen, Yuanyuan, Bi, Lijun, Zhang, Xian-En“…We found that both the full-length spike and its receptor-binding domain (RBD) of Omicron bind to hACE2 with an affinity similar to that of the wild-type. …”
Publicado 2023
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4179“…Modelling data show that either a direct or proximal binding to RBD sites by aptamer or heparin prevents the ACE2 binding. …”
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4180por Uzun, Günalp, Müller, Rebecca, Althaus, Karina, Becker, Matthias, Marsall, Patrick, Junker, Daniel, Nowak-Harnau, Stefanie, Schneiderhan-Marra, Nicole, Klüter, Harald, Schrezenmeier, Hubert, Bugert, Peter, Bakchoul, Tamam“…Clinical parameters were recorded and anti-SARS-CoV-2 antibody levels (Spike Trimer, Receptor Binding Domain (RBD), S1, S2 and nucleocapsid protein) as well as ACE2 binding inhibition were measured. …”
Publicado 2023
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