-
4321por Eslami, Narges, Aghbash, Parisa Shiri, Shamekh, Ali, Entezari-Maleki, Taher, Nahand, Javid Sadri, Sales, Abolfazl Jafari, Baghi, Hossein Bannazadeh“…The attachment of receptor-binding domains (RBD) of the spike proteins (S) to the host cell’s membrane, with or without the help of other cellular components such as proteases and especially co-receptors, is required for the first stage of its pathogenesis. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4322por Bonnet, Benjamin, Chabrolles, Hélène, Archimbaud, Christine, Brebion, Amélie, Cosme, Justine, Dutheil, Frédéric, Lambert, Céline, Junda, Maud, Mirand, Audrey, Ollier, Amandine, Pereira, Bruno, Regagnon, Christel, Vidal, Magali, Evrard, Bertrand, Henquell, Cécile“…We analysed the humoral and T-cell immune responses in 110 healthcare workers (HCWs) vaccinated according to the manufacturer’s recommended schedule of dose 2 three weeks after dose 1 from a prospective on-going cohort in early 2021, 3 and 6 months after full vaccination with the BNT162b2 mRNA vaccine. Anti-RBD IgG titres were lower in HCWs over 60 years old 3 months after the second dose (p=0.03) and declined in all the subjects between 3 and 6 months with a median percentage change of -58.5%, irrespective of age and baseline comorbidities. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4323por Boudewijns, Robbert, Pérez, Patricia, Lázaro-Frías, Adrián, Van Looveren, Dominique, Vercruysse, Thomas, Thibaut, Hendrik Jan, Weynand, Birgit, Coelmont, Lotte, Neyts, Johan, Astorgano, David, Montenegro, Dolores, Puentes, Eugenia, Rodríguez, Esteban, Dallmeier, Kai, Esteban, Mariano, García-Arriaza, Juan“…Immunization with one or two doses of MVA-S elicited high titers of S- and receptor-binding domain (RBD)-binding IgG antibodies and neutralizing antibodies against parental SARS-CoV-2 and VoC alpha, beta, gamma, delta, and omicron. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4324por Jiang, Sheng, Wu, Shuting, Zhao, Gan, He, Yue, Bao, Linlin, Liu, Jiangning, Qin, Chuan, Hou, Jiawang, Ding, Yuan, Cheng, Alex, Jiang, Brian, Wu, John, Yan, Jian, Humeau, Laurent, Patella, Ami, Weiner, David B., Broderick, Kate, Wang, Bin“…Here, we show optimization in mice generating a pGX-9501 DNA vaccine encoding full-length spike protein, which results in induction of potent humoral and cellular immune responses, including neutralizing antibodies, that block hACE2-RBD binding of live CoV2 virus in vitro. Optimization resulted in improved induction of cellular immunity by pGX-9501 as demonstrated by increased IFN-γ expression in both CD8+ and CD4 + T cells and this was associated with more robust antiviral CTL responses compared to unoptimized constructs. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4325por Lupitha, Santhik Subhasingh, Darvin, Pramod, Chandrasekharan, Aneesh, Varadarajan, Shankara Narayanan, Divakaran, Soumya Jaya, Easwaran, Sreekumar, Nelson-Sathi, Shijulal, Umasankar, Perunthottathu K, Jones, Sara, Joseph, Iype, Pillai, Madhavan Radhakrishna, Santhoshkumar, Thankayyan Retnabai“…The first approach demonstrates single-color fluorescent imaging of ACE2-GFPSpark binding to His-tagged S1-Receptor Binding Domain (RBD-His) immobilized beads. The second approach is dual-color imaging of soluble ACE2-GFPSpark to S1-Orange Fluorescent Protein (S1-OFPSpark) beads. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4326por Khan, Taimoor, Khan, Abbas, Ansari, Jawad Khaliq, Najmi, Muzammil Hasan, Wei, Dong-Qing, Muhammad, Khalid, Waheed, Yasir“…In this study, putative immunogenic epitopes including Cytotoxic T Lymphocytes (CTLs), Helper T Lymphocytes (HTLs), and Beta-cells (B-cells) were predicted for each RBD-containing region of the three highly pathogenic hCoVs. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4327por McCafferty, Sean, Haque, A.K.M. Ashiqul, Vandierendonck, Aster, Weidensee, Brian, Plovyt, Magalie, Stuchlíková, Magdalena, François, Nathalie, Valembois, Sophie, Heyndrickx, Leo, Michiels, Johan, Ariën, Kevin K., Vandekerckhove, Linos, Abdelnabi, Rana, Foo, Caroline S., Neyts, Johan, Sahu, Itishri, Sanders, Niek N.“…In this paper, we demonstrate that 1 μg of an LNP-formulated dual-antigen self-amplifying RNA vaccine (ZIP1642), encoding both the S-RBD and N antigen, elicits considerably higher neutralizing antibody titers against Wuhan-like Beta B.1.351 and Delta B.1.617.2 SARS-CoV-2 variants compared to those of convalescent patients. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4328por de Souza, Anacleto Silva, de Freitas Amorim, Vitor Martins, Guardia, Gabriela D. A., dos Santos, Filipe F., Ulrich, Henning, Galante, Pedro A. F., de Souza, Robson Francisco, Guzzo, Cristiane Rodrigues“…In this review, we dissect the impacts of the Spike mutations on biological parameters such as (1) the increase in Spike binding affinity to hACE2; (2) bound time for the receptor to be cleaved by the proteases; (3) how mutations associate with the increase in RBD up-conformation state in the Spike ectodomain; (4) expansion of uncleaved Spike protein in the virion particles; (5) increment in Spike concentration per virion particles; and (6) evasion of the immune system. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4329por Bugatti, Antonella, Filippini, Federica, Bardelli, Marta, Zani, Alberto, Chiodelli, Paola, Messali, Serena, Caruso, Arnaldo, Caccuri, Francesca“…All Severe Acute Respiratory Syndrome Virus type 2 (SARS-CoV-2) lineages own an RGD motif (aa 403–405) in their receptor binding domain (RBD). We recently showed that SARS-CoV-2 gains access into primary human lung microvascular endothelial cells (HL-mECs) lacking Angiotensin-converting enzyme 2 (ACE2) expression through this conserved RGD motif. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4330por Wang, Pan, Yang, Nan, Xue, Yuting, Zhou, Jiansuo, Wu, Yonghua, Wang, Tiancheng, Cui, Liyuan“…The level and seroprevalence of serum anti-SARS-CoV-2 receptor-binding domain (RBD) IgM/IgG and the association with peripheral blood leukocytes classification were investigated. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4331por Ozawa, Tatsuhiko, Tani, Hideki, Anraku, Yuki, Kita, Shunsuke, Igarashi, Emiko, Saga, Yumiko, Inasaki, Noriko, Kawasuji, Hitoshi, Yamada, Hiroshi, Sasaki, so-Ichiro, Somekawa, Mayu, Sasaki, Jiei, Hayakawa, Yoshihiro, Yamamoto, Yoshihiro, Morinaga, Yoshitomo, Kurosawa, Nobuyuki, Isobe, Masaharu, Fukuhara, Hideo, Maenaka, Katsumi, Hashiguchi, Takao, Kishi, Hiroyuki, Kitajima, Isao, Saito, Shigeru, Niimi, Hideki“…The structural analyses revealed that antibody UT28K Fab and SARS-CoV-2 RBD protein interactions were mainly chain-dominated antigen-antibody interactions. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4332por Garcia-Valtanen, Pablo, Hope, Christopher M., Masavuli, Makutiro G., Yeow, Arthur Eng Lip, Balachandran, Harikrishnan, Mekonnen, Zelalem A., Al-Delfi, Zahraa, Abayasingam, Arunasingam, Agapiou, David, Stella, Alberto Ospina, Aggarwal, Anupriya, Bouras, George, Gummow, Jason, Ferguson, Catherine, O’Connor, Stephanie, McCartney, Erin M., Lynn, David J., Maddern, Guy, Gowans, Eric J., Reddi, Benjamin A.J., Shaw, David, Kok-Lim, Chuan, Beard, Michael R., Weiskopf, Daniela, Sette, Alessandro, Turville, Stuart G., Bull, Rowena A., Barry, Simon C., Grubor-Bauk, Branka“…In this cohort, ancestral, receptor-binding domain (RBD)-specific antibody and circulating memory B cell levels are conserved in most individuals, and yet serum neutralization against live B.1.1.529 (Omicron) is completely abrogated and significantly reduced for other VOCs. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4333por Shapira, Guy, Abu Hamad, Ramzia, Weiner, Chen, Rainy, Nir, Sorek‐Abramovich, Reut, Benveniste‐Levkovitz, Patricia, Rock, Rachel, Avnat, Eden, Levtzion‐Korach, Osnat, Bar Chaim, Adina, Shomron, Noam“…Serum samples were obtained from 25 897 participants and assayed for anti‐SARS‐CoV‐2 spike protein RBD IgG antibodies. The cohort was composed of newly vaccinated BNT162b2 recipients, in the first month or 6 months after vaccination, COVID‐19 patients and a general sample of the Israeli population. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4334por Ga, Eulhae, Won, Yongkwan, Hwang, Jaehyun, Moon, Suyun, Yeom, Minju, Lyoo, Kwangsoo, Song, Daesub, Han, Jeonghee, Na, Woonsung“…To counteract reverse zoonosis, we developed the first COVID-19 subunit vaccines for dogs, which are representative companion animals, and the vaccine includes the SARS-CoV-2 recombinant protein of whole S1 protein and the receptor-binding domain (RBD). A subunit vaccine is a vaccine developed by purifying only the protein region that induces an immune response instead of the whole pathogen. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4335por Demirhindi, Hakan, Mete, Burak, Tanir, Ferdi, Kara, Ertan, Kibar, Filiz, Cetiner, Salih, Candevir, Aslihan, Akti, Sukriye Ece“…They were divided into three cohorts after the third month: Cohort-I (CV/CV); Cohort-II (CV/CV/CV); and Cohort-III (CV/CV/BNT). Anti-S-RBD-IgG and total anti-spike/anti-nucleocapsid-IgG antibody concentrations were examined in vaccinated health workers at the 1st, 3rd, and 6th months following the second dose of the vaccination. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4336por Teplensky, Michelle H., Distler, Max E., Kusmierz, Caroline D., Evangelopoulos, Michael, Gula, Haley, Elli, Derek, Tomatsidou, Anastasia, Nicolaescu, Vlad, Gelarden, Ian, Yeldandi, Anjana, Batlle, Daniel, Missiakas, Dominique, Mirkin, Chad A.“…Using COVID-19 as a topical example of an infectious disease with an urgent need for rapid vaccine development, we designed an antiviral SNA vaccine, encapsulating the receptor-binding domain (RBD) subunit into a liposome and decorating the core with a dense shell of CpG motif toll-like receptor 9 agonist oligonucleotides. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4337por Liang, Huang, Zheng, Peiyan, Wang, Qian, Deng, Yijun, Liang, Dan, Yi, Haisu, Cheng, Yuanyi, Zhao, Xinwei, Ma, Jing, Yang, Yidong, Hu, Peiyu, Zheng, Pingqian, Zhang, Yudi, Huang, Shuangshuang, Lin, Xiancheng, Ke, Changwen, Niu, Xuefeng, Sun, Baoqing, Chen, Ling“…After receiving the second vaccination, antibodies against RBD of SARS-CoV-2 Wuhan, Beta, Delta, and recently emerged Omicron are significantly higher in SARS-CoV experienced vaccinees than in SARS-CoV naïve vaccinees. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4338por Pouremamali, Amir, Babaei, Abouzar, Malekshahi, Somayeh Shatizadeh, Abbasi, Ardeshir, Rafiee, Nastaran“…Binding the receptor-binding domain (RBD) of S protein to angiotensin-converting enzyme 2 (ACE 2), a cell-surface receptor, mediates virus entry into cells; thus, understanding the basics of ACE2 and S protein, their interactions, and ACE2 targeting could be a potent priority for inhibition of virus infection. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4339por Kamel, Manal, Maher, Sara, El-Baz, Hanan, Salah, Faten, Sayyouh, Omar, Demerdash, Zeinab“…S1 protein receptor-binding domain (RBD) nanobodies were efficiently conjugated with 40 nm gold nanoparticles (AuNPs) and employed as antigen detection probes in the developed system, while recombinant S1 monoclonal antibodies (S1mAbs) were employed as antigen capture probes. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
4340por Calcoen, Bas, Callewaert, Nico, Vandenbulcke, Aline, Kerstens, Winnie, Imbrechts, Maya, Vercruysse, Thomas, Dallmeier, Kai, Van Weyenbergh, Johan, Maes, Piet, Bossuyt, Xavier, Zapf, Dorinja, Dieckmann, Kersten, Callebaut, Kim, Thibaut, Hendrik Jan, Vanhoorelbeke, Karen, De Meyer, Simon F., Maes, Wim, Geukens, Nick“…Secondly, antibody functionality was analyzed via in vitro binding inhibition and pseudovirus neutralization and circulating receptor-binding domain (RBD)-specific B cells were assessed. Moreover, the induction of SARS-CoV-2-specific T cells was investigated by an interferon-γ release assay combined with flowcytometric profiling of activated CD4(+) and CD8(+) T cells. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto