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4441por Flores-Alanis, Alejandro, Sandner-Miranda, Luisa, Delgado, Gabriela, Cravioto, Alejandro, Morales-Espinosa, Rosario“…Moreover, it is likely that the basic function of the receptor-binding domain (RBD) of S protein was acquired by the SARS-CoV-2 from the MP789 pangolin CoV by recombination and it has been highly conserved.…”
Publicado 2020
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4442“…In fact, in a single ACE2 molecule, 34 lysine residues are present in the extracellular portion, and at least one of these is co-involved in a fundamental hydrogen-bond interaction with the SARS-CoV-2 receptor binding domain (RBD). The worse outcome of COVID-19 in people with diabetes could be related to the non-enzymatic glycation that triggers the activity of ACE2. …”
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4443por Chaudhry, Sundas Nasir, Hazafa, Abu, Mumtaz, Muhummad, Kalsoom, Ume, Abbas, Saima, Kainaat, Amna, Bilal, Shahid, Zafar, Nauman, Siddique, Aleena, Zafar, Ayesha“…In December 2019, a novel virus, namely COVID-19 caused by SARS-CoV-2, developed from Wuhan, (Hubei territory of China) used its viral spike glycoprotein receptor-binding domain (RBD) for the entrance into a host cell by binding with ACE-2 receptor and cause acute respiratory distress syndrome (ARDS). …”
Publicado 2020
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4444por Yu, Shaopeng, Zhu, Yuying, Xu, Jiaruo, Yao, Guangtao, Zhang, Pei, Wang, Mengge, Zhao, Yongfang, Lin, Guoqiang, Chen, Hongzhuan, Chen, Lili, Zhang, Jiange“…Surface plasmon resonance (SPR) assays and NanoBit assays were used to verify the predicated compounds with their binding activities to spike proteins and inhibitory activities on the SARS-CoV-2 RBD/ACE2 interaction, respectively. Furthermore, an MTT assay was used to evaluate the cell toxicities of active compounds. …”
Publicado 2021
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4445por Zhang, Qiang, Zhang, Huajun, Gao, Jindong, Huang, Kun, Yang, Yong, Hui, Xianfeng, He, Xinglin, Li, Chengfei, Gong, Wenxiao, Zhang, Yufei, Zhao, Ya, Peng, Cheng, Gao, Xiaoxiao, Chen, Huanchun, Zou, Zhong, Shi, Zheng-Li, Jin, Meilin“…Fifteen sera collected after the outbreak were positive for the receptor binding domain (RBD) of SARS-CoV-2 by indirect enzyme linked immunosorbent assay (ELISA). …”
Publicado 2020
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4446por Xiong, Hua-Long, Wu, Yang-Tao, Cao, Jia-Li, Yang, Ren, Liu, Ying-Xia, Ma, Jian, Qiao, Xiao-Yang, Yao, Xiang-Yang, Zhang, Bao-Hui, Zhang, Ya-Li, Hou, Wang-Heng, Shi, Yang, Xu, Jing-Jing, Zhang, Liang, Wang, Shao-Juan, Fu, Bao-Rong, Yang, Ting, Ge, Sheng-Xiang, Zhang, Jun, Yuan, Quan, Huang, Bao-Ying, Li, Zhi-Yong, Zhang, Tian-Ying, Xia, Ning-Shao“…Seven neutralizing monoclonal antibodies targeting the receptor binding domain (RBD) of the SARS-CoV-2 S protein were obtained. …”
Publicado 2020
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4447“…Recent studies have reported that similar to SARS-CoV, this strain expresses a spike protein (S) with a receptor binding domain (RBD) that binds to angiotensin-converting enzyme 2 (ACE2) – an enzyme expressed mostly in the endothelium, kidneys, heart, gastrointestinal tract and lungs – to facilitate viral entry and intracellular replication. …”
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4448“…This interaction involves a receptor binding domain (RBD) located at the center of the S trimer, whereas the lateral N-terminal domain (NTD) displays a flat ganglioside binding site that enables the virus to bind to lipid rafts of the plasma membrane, where the ACE-2 receptor resides. …”
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4449por Padhi, Srichandan, Masi, Marco, Chourasia, Rounak, Rajashekar, Yallappa, Rai, Amit Kumar, Evidente, Antonio“…Molecular docking against the receptor binding domain (RBD) of spike glycoprotein (S1) and the main protease (M(pro)) exposed the compounds having better binding affinity to main protease as compared to the S1 receptor binding domain. …”
Publicado 2021
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4450por Chen, Chih-Chieh, Hsu, Chia-Yi, Lin, Hsiao-Yun, Zeng, Hong-Qi, Cheng, Kuang-Hung, Wu, Chia-Wei, Tsai, Eing-Mei, Hsieh, Tsung-Hua“…We found decreased binding to the Raf1 RBD by KRAS(G12D/K104Q) and reduced cell growth, invasion and migration. …”
Publicado 2020
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4451“…The affinity of the spike protein receptor binding domain (RBD) of SARS-CoV-2 for human ACE2 (hACE2) largely determines the degree of clinical symptoms after infection by SARS-CoV-2. …”
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4452por Alouane, Tarek, Laamarti, Meriem, Essabbar, Abdelomunim, Hakmi, Mohammed, Bouricha, El Mehdi, Chemao-Elfihri, M. W., Kartti, Souad, Boumajdi, Nasma, Bendani, Houda, Laamarti, Rokia, Ghrifi, Fatima, Allam, Loubna, Aanniz, Tarik, Ouadghiri, Mouna, El Hafidi, Naima, El Jaoudi, Rachid, Benrahma, Houda, Attar, Jalil El, Mentag, Rachid, Sbabou, Laila, Nejjari, Chakib, Amzazi, Saaid, Belyamani, Lahcen, Ibrahimi, Azeddine“…Moreover, 36 non-synonymous mutations were identified in the receptor-binding domain (RBD) of the spike protein with a low prevalence (<1%) across all genomes, of which only four could potentially enhance the binding of the SARS-CoV-2 spike protein to the human ACE2 receptor. …”
Publicado 2020
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4453por Custódio, Tânia F., Das, Hrishikesh, Sheward, Daniel J., Hanke, Leo, Pazicky, Samuel, Pieprzyk, Joanna, Sorgenfrei, Michèle, Schroer, Martin A., Gruzinov, Andrey Yu., Jeffries, Cy M., Graewert, Melissa A., Svergun, Dmitri I., Dobrev, Nikolay, Remans, Kim, Seeger, Markus A., McInerney, Gerald M., Murrell, Ben, Hällberg, B. Martin, Löw, Christian“…Here, we report the rapid isolation and characterization of nanobodies from a synthetic library, known as sybodies (Sb), that target the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. Several binders with low nanomolar affinities and efficient neutralization activity were identified of which Sb23 displayed high affinity and neutralized pseudovirus with an IC(50) of 0.6 µg/ml. …”
Publicado 2020
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4454por Carrillo, Jorge, Izquierdo-Useros, Nuria, Ávila-Nieto, Carlos, Pradenas, Edwards, Clotet, Bonaventura, Blanco, Julià“…Of particularly interest, the antibodies against domain of the Spike that interacts with the cellular receptor ACE2, known as the receptor binding domain (RBD), are present in most infected individuals and are block viral entry and infectivity. …”
Publicado 2021
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4455por Lu, Maolin, Uchil, Pradeep D., Li, Wenwei, Zheng, Desheng, Terry, Daniel S., Gorman, Jason, Shi, Wei, Zhang, Baoshan, Zhou, Tongqing, Ding, Shilei, Gasser, Romain, Prévost, Jérémie, Beaudoin-Bussières, Guillaume, Anand, Sai Priya, Laumaea, Annemarie, Grover, Jonathan R., Liu, Lihong, Ho, David D., Mascola, John R., Finzi, Andrés, Kwong, Peter D., Blanchard, Scott C., Mothes, Walther“…Conformational preferences observed upon exposure to convalescent plasma or antibodies suggest mechanisms of neutralization involving either competition with hACE2 for binding to the receptor-binding domain (RBD) or allosteric interference with conformational changes required for entry. …”
Publicado 2020
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4456por Krishnamurthy, Hari Krishnan, Jayaraman, Vasanth, Krishna, Karthik, Rajasekaran, Karenah E., Wang, Tianhao, Bei, Kang, Rajasekaran, John J., Yaskin, Inna, Rai, Alex J., Choung, Rok Seon, Murray, Joseph A.“…METHODS: A protein microarray technology was used to detect the plurality of antibody response to four novel antigens namely S1 glycoprotein, Receptor binding domain (RBD), S2 glycoprotein and Nucleoprotein of the novel coronavirus named SARS-CoV2 using serum samples. …”
Publicado 2020
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4457por da Costa, Camila B.P., Martins, Francislene J., da Cunha, Luis E.R., Ratcliffe, Norman A., Cisne de Paula, Rafael, Castro, Helena C.“…Promising antigens are also indicated such as the receptor-binding domain (RBD) in SARS-CoV-2 S protein, which is already in phase 2/3 clinical trial, and the trimeric protein S, which was shown to be up to 150 times more potent than the serum from convalescent donors. …”
Publicado 2021
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4458por Xi, Cecy R, Di Fazio, Arianna, Nadvi, Naveed Ahmed, Patel, Karishma, Xiang, Michelle Sui Wen, Zhang, Hui Emma, Deshpande, Chandrika, Low, Jason K K, Wang, Xiaonan Trixie, Chen, Yiqian, McMillan, Christopher L D, Isaacs, Ariel, Osborne, Brenna, Vieira de Ribeiro, Ana Júlia, McCaughan, Geoffrey W, Mackay, Joel P, Church, W Bret, Gorrell, Mark D“…The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor-binding domain (RBD) were measured using surface plasmon resonance and ELISA. …”
Publicado 2020
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4459por Gopinath, Krishnasamy, Jokinen, Elmeri M., Kurkinen, Sami T., Pentikäinen, Olli T.“…Three compounds, ZINC000002128789, ZINC000002159944 and SN00059335, showed better stability in all MD runs, of which ZINC000002128789 was predicted to have the highest binding affinity, suggesting that it could be effective modulator in RBD-ACE2 interface to prevent SARS-CoV-2 infection. …”
Publicado 2020
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4460por Sugiyama, Takefumi, Yamamoto, Haruka, Kon, Tetsuo, Chaya, Taro, Omori, Yoshihiro, Suzuki, Yutaka, Abe, Kentaro, Watanabe, Dai, Furukawa, Takahisa“…We then performed a pull-down assay using Rhotekin-RBD and observed GEF activity of Arhgef33 against RhoA. …”
Publicado 2020
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