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4481por Dejnirattisai, Wanwisa, Zhou, Daming, Supasa, Piyada, Liu, Chang, Mentzer, Alexander J., Ginn, Helen M., Zhao, Yuguang, Duyvesteyn, Helen M.E., Tuekprakhon, Aekkachai, Nutalai, Rungtiwa, Wang, Beibei, López-Camacho, César, Slon-Campos, Jose, Walter, Thomas S., Skelly, Donal, Costa Clemens, Sue Ann, Naveca, Felipe Gomes, Nascimento, Valdinete, Nascimento, Fernanda, Fernandes da Costa, Cristiano, Resende, Paola Cristina, Pauvolid-Correa, Alex, Siqueira, Marilda M., Dold, Christina, Levin, Robert, Dong, Tao, Pollard, Andrew J., Knight, Julian C., Crook, Derrick, Lambe, Teresa, Clutterbuck, Elizabeth, Bibi, Sagida, Flaxman, Amy, Bittaye, Mustapha, Belij-Rammerstorfer, Sandra, Gilbert, Sarah C., Carroll, Miles W., Klenerman, Paul, Barnes, Eleanor, Dunachie, Susanna J., Paterson, Neil G., Williams, Mark A., Hall, David R., Hulswit, Ruben J.G., Bowden, Thomas A., Fry, Elizabeth E., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.“…We show that, surprisingly, P.1 is significantly less resistant to naturally acquired or vaccine-induced antibody responses than B.1.351, suggesting that changes outside the receptor-binding domain (RBD) impact neutralization. Monoclonal antibody (mAb) 222 neutralizes all three variants despite interacting with two of the ACE2-binding site mutations. …”
Publicado 2021
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4482“…Essential tremor-Parkinson’s disease patients were more likely to have RBD symptoms. There was no significant difference in cognitive impairment between essential tremor plus and essential tremor-Parkinson’s disease patients. …”
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4483por Padoan, Andrea, Dall'Olmo, Luigi, Rocca, Foscarina della, Barbaro, Francesco, Cosma, Chiara, Basso, Daniela, Cattelan, Annamaria, Cianci, Vito, Plebani, Mario“…METHODS: 163 health care workers (HCW) of the Padua University Hospitals, who underwent a complete vaccination campaign with BNT162b2 vaccine were asked to collect serum samples at 12 (t12) and 28 (t28) days after the first inoculum to allow the measurement of SARS-CoV-2 Antibodies (Ab) using chemiluminescent assays against the spike (S) protein and the Receptor Binding Domain (RBD) of the virus, respectively. RESULTS: Significant differences were found at t12 for infection-naïve and subjects with previous-natural infection who present higher values of specific antibodies, while no significant differences have been found between t12 and t28. …”
Publicado 2021
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4484por Egia-Mendikute, Leire, Bosch, Alexandre, Prieto-Fernández, Endika, Lee, So Young, Jiménez-Lasheras, Borja, García del Río, Ana, Antoñana-Vildosola, Asier, Bruzzone, Chiara, Bizkarguenaga, Maider, Embade, Nieves, Gil-Redondo, Rubén, Martínez-Chantar, María Luz, López-Hoyos, Marcos, Abrescia, Nicola G. A., Mato, José M., Millet, Óscar, Palazón, Asís“…For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). …”
Publicado 2021
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4485por Kathiravan, Muthu Kumaradoss, Radhakrishnan, Srimathi, Namasivayam, Vigneshwaran, Palaniappan, Senthilkumar“…Spike is proven to be an interesting target for developing vaccines and in particular, the RBD-single chain dimer has shown initial success. …”
Publicado 2021
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4486por Morassi, Mauro, Palmerini, Francesco, Nici, Stefania, Magni, Eugenio, Savelli, Giordano, Guerra, Ugo Paolo, Chieregato, Matteo, Morbelli, Silvia, Vogrig, Alberto“…None of the patients had previous history of parkinsonian signs/symptoms, and none had prodromal features of Parkinson’s disease (hyposmia or RBD). Both developed a rapidly progressive form of atypical parkinsonism along with distinctive features suggestive of encephalitis. …”
Publicado 2021
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4487por DiMuzio, Jillian M., Heimbach, Baron C., Howanski, Raymond J., Dowling, John P., Patel, Nirja B., Henriquez, Noeleya, Nicolescu, Chris, Nath, Mitchell, Polley, Antonio, Bingaman, Jamie L., Smith, Todd, Harman, Benjamin C., Robinson, Matthew K., Morin, Michael J., Nikitin, Pavel A.“…Further, all but one of the six COVID-19 convalescent patients produced class-switched antibodies to a soluble form of the receptor-binding domain (RBD) of S protein. Functional properties of anti-Spike antibodies were confirmed in a pseudovirus neutralization assay. …”
Publicado 2021
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4488por Majdoubi, Abdelilah, Michalski, Christina, O’Connell, Sarah E., Dada, Sarah, Narpala, Sandeep, Gelinas, Jean, Mehta, Disha, Cheung, Claire, Winkler, Dirk F.H., Basappa, Manjula, Liu, Aaron C., Görges, Matthias, Barakauskas, Vilte E., Irvine, Mike, Mehalko, Jennifer, Esposito, Dominic, Sekirov, Inna, Jassem, Agatha N., Goldfarb, David M., Pelech, Steven, Douek, Daniel C., McDermott, Adrian B., Lavoie, Pascal M.“…Using a highly sensitive multiplex assay and positive/negative thresholds established in infants in whom maternal antibodies have waned, we determined that more than 90% of uninfected adults showed antibody reactivity against the spike protein, receptor-binding domain (RBD), N-terminal domain (NTD), or the nucleocapsid (N) protein from SARS-CoV-2. …”
Publicado 2021
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4489por Lewin, Antoine, Therrien, Roseline, De Serres, Gaston, Grégoire, Yves, Perreault, Josée, Drouin, Mathieu, Fournier, Marie-Josée, Tremblay, Tony, Beaudoin, Julie, Beaudoin-Bussières, Guillaume, Prévost, Jérémie, Gendron-Lepage, Gabrielle, Finzi, Andrés, Bernier, France, Bazin, Renée, Germain, Marc, Delage, Gilles“…Residual blood samples from donations collected between May 25 and July 9, 2020 (well before vaccination rollout) in the province of Québec were tested for anti-Spike RBD antibodies by ELISA. Seropositive donors and a control group of seronegative donors were questioned about prior COVID-19 symptoms. …”
Publicado 2021
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4490por Nag, Kakon, Chandra Baray, Juwel, Rahman Khan, Maksudur, Mahmud, Asif, Islam, Jikrul, Myti, Sanat, Ali, Rostum, Haq Sarker, Enamul, Kumar, Samir, Hossain Chowdhury, Mobarak, Roy, Rony, Islam, Faqrul, Barman, Uttam, Khan, Habiba, Chakraborty, Sourav, Badsha, Alam, Hossain, Manik, Ahammad, Shamim, Rahman Chowdhury, Mashfiqur, Ghosh, Polash, Islam Shimul, Rayhanul, Ahmmed, Ronzu, Hussain Bhuiya, Eleus, Kumar Biswas, Bipul, Mohiuddin, Mohammad, Sultana, Naznin“…The surface plasmon resonance (SPR) data with spike protein (S) revealed that immunization generated specific antibody pools against the whole extracellular domain (RBD and S2) of the spike protein. The anti-sera and purified IgGs from immunized mice neutralized SARS-CoV-2-pseudoviruses in ACE2-expressing HEK293 cells in a dose dependent manner. …”
Publicado 2021
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4491por Turgeon, Coleman T., Sanders, Karen A., Rinaldo, Piero, Granger, Dane, Hilgart, Heather, Matern, Dietrich, Theel, Elitza S.“…METHODS: The Luminex xMAP SARS-CoV-2 Multi-Antigen assay utilizes magnetic beads labeled with three viral antigens (nucleocapsid [NC], receptor binding domain [RBD], spike S1 subunit) to detect anti-viral IgG-class antibodies, and has Food and Drug Administration (FDA) Emergency Use Authorization (EUA) for use in serum and plasma. …”
Publicado 2021
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4492por Mukhopadhyay, Labanya, Yadav, Pragya D., Gupta, Nivedita, Mohandas, Sreelekshmy, Patil, Deepak Y., Shete-Aich, Anita, Panda, Samiran, Bhargava, Balram“…INTERPRETATION & CONCLUSIONS: Our findings highlighted onset of quick immunogenicity and protective efficacy of mRNA-1273, followed by Ad26.CoV2.S, NVX-CoV2373, BNT162b2, RBD and BBV152 vaccine candidates in preclinical trials as compared to the others. …”
Publicado 2021
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4493por Yeo, Joo Guan, Leong, Jing Yao, Tay, Shi Huan, Nadua, Karen Donceras, Anderson, Danielle E., Lim, Amanda Jin Mei, Ng, Xiang Wen, Poh, Su Li, Guo, Dianyan, Yaung, Katherine Nay, Kumar, Pavanish, Wasser, Martin, Hazirah, Sharifah Nur, Sutamam, Nursyuhadah, Chua, Camillus Jian Hui, Qui, Martin, Foo, Randy, Gamage, Akshamal Mihiranga, Yeo, Kee Thai, Ramakrishna, Lakshmi, Arkachaisri, Thaschawee, Young, Barnaby E., Lye, David Chien, Wang, Lin-Fa, Chong, Chia Yin, Tan, Natalie Woon Hui, Li, Jiahui, Kam, Kai-Qian, Ginhoux, Florent, Thoon, Koh Cheng, Chan, Jerry Kok Yen, Yung, Chee Fu, Albani, Salvatore“…Both components were reactive against a peptide pool covering the receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein. We also observed expansion of IFNγ(+) memory CD4(+) T cells and virus-specific follicular helper T (T(FH)) cells. …”
Publicado 2021
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4494por Giofrè, Salvatore Vincenzo, Napoli, Edoardo, Iraci, Nunzio, Speciale, Antonio, Cimino, Francesco, Muscarà, Claudia, Molonia, Maria Sofia, Ruberto, Giuseppe, Saija, Antonella“…On the contrary, despite the good affinity with the spike protein RBD site, all the selected phytochemicals lose contact with the amino acid residues over the course of 120ns-long molecular dynamics simulations therefore suggesting they scarcely can interfere in SARS-CoV-2 binding to the ACE2 receptor. …”
Publicado 2021
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4495por Spratt, Austin N., Kannan, Saathvik R., Woods, Lucas T., Weisman, Gary A., Quinn, Thomas P., Lorson, Christian L., Sönnerborg, Anders, Byrareddy, Siddappa N., Singh, Kamal“…However, reported greater binding affinity of N501Y Spike with ACE2 does not seem to be entirely due to increased hydrophobic interactions, given that Spike mutation R417T in P.1 or K417N in B.1.351 results in the loss of a salt-bridge interaction between ACE2 and S-RBD. The calculated change in free energy did not provide a clear trend of S protein stability of mutations in the variants. …”
Publicado 2021
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4496por Heggestad, Jacob T., Kinnamon, David S., Olson, Lyra B., Liu, Jason, Kelly, Garrett, Wall, Simone A., Oshabaheebwa, Solomon, Quinn, Zachary, Fontes, Cassio M., Joh, Daniel Y., Hucknall, Angus M., Pieper, Carl, Anderson, Jack G., Naqvi, Ibtehaj A., Chen, Lingye, Que, Loretta G., Oguin, Thomas, Nair, Smita K., Sullenger, Bruce A., Woods, Christopher W., Burke, Thomas W., Sempowski, Gregory D., Kraft, Bryan D., Chilkoti, Ashutosh“…Here, we report on a microfluidic POC test that can profile the antibody response against multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens—spike S1 (S1), nucleocapsid (N), and the receptor binding domain (RBD)—simultaneously from 60 μl of blood, plasma, or serum. …”
Publicado 2021
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4497por Muthumani, Kar, Xu, Ziyang, Jeong, Moonsup, Maslow, Joel N., Kalyanaraman, Vaniambadi S., Srinivasan, Alagarsamy“…The inclusion of tests specific to SARS-CoV-2 (such as RBD of spike protein) could overcome these limitations.…”
Publicado 2021
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4498por Karthika, Thankamani, Joseph, Jeswin, Das, V. R. Akshay, Nair, Niranjana, Charulekha, Packirisamy, Roji, Melvin Daniel, Raj, V. Stalin“…Complete cytoplasmic domain deleted ACE2 (∆cytACE2) was properly synthesized and presented on the surface of HEK293T and BHK21 cells like wtACE2. The SARS-CoVs S1 or RBD of spike protein binds and colocalizes with the receptors followed by internalization into the host cells. …”
Publicado 2021
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4499por Wilson, Patrick, Changrob, Siriruk, Fu, Yanbin, Guthmiller, Jenna, Halfmann, Peter, Li, Lei, Stamper, Christopher, Dugan, Haley, Accola, Molly, Rehrauer, William, Zheng, Nai-Ying, Huang, Min, Wang, Jiaolong, Erickson, Steven, Utset, Henry, Graves, Hortencia, Amanat, Fatima, Sather, D. Noah, Krammer, Florian, Kawaoka, Yoshihiro“…Viral variants harboring mutations at K417, E484 and N501 could escape most of the highly potent antibodies against the receptor binding domain (RBD). Despite this, we identified 12 neutralizing mAbs against three distinct regions of the spike protein that neutralize SARS-CoV-2 and the variants of concern, including B.1.1.7 (alpha), P.1 (gamma) and B.1.617.2 (delta). …”
Publicado 2021
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4500“…The concordance and rigidity ratios of multiple mutation strains such as B.1.617.2 against the wild-type one at the receptor-binding domain (RBD) and receptor-binding motif (RBM) regions provide a good indication of the transmissibility and neutralization escape ability except for binding affinity of mutation sites such as N501Y.…”
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