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4601por Lee, Ha Neul, Lee, Juhee, Kang, Yoo Kyung, Lee, Joo Hoon, Yang, Seungju, Chung, Hyun Jung“…Furthermore, applying the RCA-LFA method using specifically designed probes for RNA-dependent RNA polymerase (RdRp) and receptor binding domain (RBD) gene for SARS-CoV-2, which demonstrated feasibility of the method for viral gene targets. …”
Publicado 2022
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4602por Stamoula, Eleni, Sarantidi, Eleana, Dimakopoulos, Vasilis, Ainatzoglou, Alexandra, Dardalas, Ioannis, Papazisis, Georgios, Kontopoulou, Konstantina, Anagnostopoulos, Athanasios K.“…A label-free quantitative proteomics LC-MS/MS approach was undertaken, and the identified proteins were analyzed using the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes) databases as well as processed by bioinformatics tools. Titers of total RBD-specific IgGs against SARS-CoV-2 were also determined using the SARS-CoV-2 IgG II Quant assay. …”
Publicado 2022
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4603por Veterini, Anna Surgean, Prakoeswa, Cita Rosita Sigit, Tinduh, Damayanti, Satuman, Satuman“…The COVID-19 cell model was given antibodies divided into three antibody level groups: sRBD levels of <3, 325.76 and 646.18. The cytotoxicity assessment examined increased levels of LDH, cytopathic activity by measuring ATP levels, and cell viability by XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) assay. …”
Publicado 2022
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4604por Jia, Liqiu, Weng, Shufeng, Wu, Jing, Tian, Xiangxiang, Zhang, Yifan, Wang, Xuyang, Wang, Jing, Yan, Dongmei, Wang, Wanhai, Fang, Fang, Zhu, Zhaoqin, Qiu, Chao, Zhang, Wenhong, Xu, Ying, Wan, Yanmin“…Similarly, we found that levels of preexisting S2 and P144-specific antibodies correlated positively with RBD binding antibody titers after two doses of inactivated SARS-CoV-2 vaccination in human. …”
Publicado 2022
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4605por Wang, Aiping, Tian, Yuanyuan, Liu, Hongliang, Ding, Peiyang, Chen, Yumei, Liang, Chao, Du, Yongkun, Jiang, Dawei, Zhu, Xifang, Yin, Jiajia, Zhang, Gaiping“…In the present study, NTD, RBD, and S2 genes were inserted into the pcDNA3.1(+) vector and designed with N-terminal 6× His-tag for fusion expression in HEK293F cells by transient transfection. …”
Publicado 2022
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4606por Villar, Margarita, Urra, José Miguel, Artigas-Jerónimo, Sara, Mazuecos, Lorena, Contreras, Marinela, Vaz-Rodrigues, Rita, Rodríguez-del-Río, Francisco J., Gortázar, Christian, de la Fuente, José“…The analysis of underrepresented immunoglobulins in PCR-positive compared to PCR-negative individuals identified vaccine-induced protective epitopes in various coronavirus proteins, including the spike receptor-binding domain RBD. Non-immunoglobulin proteins were associated with COVID-19 symptoms and biological processes. …”
Publicado 2022
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4607por Biagio, Pinchera, Rosa, Carrano, Nicola, Schiano Moriello, Fabrizio, Salemi, Amerigo, Piccione, Giulia, Zumbo, Riccardo, Scotto, Riccardo, Villari, Paolo, Romano, Lorenzo, Spirito, Ivan, Gentile“…For each patient, we evaluated IgG anti-S-RBD SARS-CoV-2 titers immediately before the administration of first COVID-19 vaccination dose, 20 days after the first dose and 40 days after the second dose. …”
Publicado 2022
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4608por Duan, Binwei, Zhang, Gongming, Wang, Wenjing, Yin, Jiming, Liu, Mengcheng, Zhang, Jing, Chen, Dexi, Ouyang, Yabo, Li, Guangming“…Time from transplant and lymphocyte count were different among the three groups. The levels of anti-RBD and anti-S1S2 decreased with decreasing neutralizing inhibition rates. …”
Publicado 2022
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4609por Norddahl, Gudmundur L., Melsted, Pall, Gunnarsdottir, Kristbjorg, Halldorsson, Gisli H., Olafsdottir, Thorunn A., Gylfason, Arnaldur, Kristjansson, Mar, Magnusson, Olafur T., Sulem, Patrick, Gudbjartsson, Daniel F., Thorsteinsdottir, Unnur, Jonsdottir, Ingileif, Stefansson, Kari“…We estimate the risk of infection for different vaccine combinations using vaccination data from 276,028 persons and 963,557 qPCR tests for 277,687 persons. We measure anti-Spike-RBD antibody levels and ACE2-Spike binding inhibitory activity in 371 persons who received one of four recommended vaccination schedules with or without an mRNA vaccine booster. …”
Publicado 2022
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4610por Muik, Alexander, Lui, Bonny Gaby, Bacher, Maren, Wallisch, Ann-Kathrin, Toker, Aras, Finlayson, Andrew, Krüger, Kimberly, Ozhelvaci, Orkun, Grikscheit, Katharina, Hoehl, Sebastian, Ciesek, Sandra, Türeci, Özlem, Sahin, Ugur“…However, neutralization by Omicron BA.1 convalescent sera depends exclusively on antibodies targeting the receptor binding domain (RBD). These findings suggest that exposure to Omicron BA.2, in contrast to BA.1 spike glycoprotein, triggers significant NTD specific recall responses in vaccinated individuals and thereby enhances the neutralization of BA.4/BA.5 sublineages. …”
Publicado 2022
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4611por Zhang, Lu, Chen, Hongquan, Yang, Su, Zhao, Yang, Shen, Xiaoyun, He, Xiaowen, Ye, Haohui, Wang, Deqin, Lou, Jiazhou, Wang, Yinshan, Wu, Shengjun“…Significant increase in neutralization breadth of 1.24 (95% confidence interval (CI), 0.89–1.59) variants was associated with a log(10) increase in neutralization titer against the wild-type. In addition, anti-RBD IgG level was identified as an excellent surrogate for positive neutralization of SARS-CoV-2 and neutralization breadth of variants. …”
Publicado 2022
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4612por Maier, Irene“…Among these, CV-N bound the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein at a dissociation constant (K(D)) of 18.6 µM (and K(D)=260 µM to RBD), which was low-affinity carbohydrate-binding as compared with the recognition of the other viral spikes. …”
Publicado 2022
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4613por Iacobucci, Ilaria, Monaco, Vittoria, Canè, Luisa, Bibbò, Francesca, Cioffi, Valentina, Cozzolino, Flora, Guarino, Alfredo, Zollo, Massimo, Monti, Maria“…In particular, the subunit S1 of the Spike glycoprotein contains the Receptor Binding Domain (RBD) and it is responsible for the interaction with the angiotensin-converting enzyme 2 (ACE2). …”
Publicado 2022
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4614por Xiang, Jiangchao, Su, Jie, Lan, Qiaoshuai, Zhao, Wenwen, Zhou, Yu, Xu, Youwei, Niu, Jun, Xia, Shuai, Qi, Qilian, Sidhu, Sachdev, Lu, Lu, Miersch, Shane, Yang, Bei“…In the receptor binding domain, a neutralizing epitope is captured in the end distal to the receptor binding site, reminiscent of the locations of the SARS-CoV-2 RBD cryptic epitopes. We also identified a neutralizing antibody that recognizes the connector domain, thus representing the first S2-directed neutralizing antibody against α-HCoVs. …”
Publicado 2022
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4615por Wang, Qinjin, Ning, Jie, Chen, Ying, Li, Bin, Shi, Liang, He, Taojun, Zhang, Fang, Chen, Xingchi, Zhai, Aixia, Wu, Chao“…As a result, antibody responses to the receptor binding domain (RBD), N, and non-structural protein 8 (NSP8) were induced following immunization by BBIBP-CorV. …”
Publicado 2022
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4616por Sammons, Rachel M., Bohanon, Amanda L., Kowtha, Anvith, Dejong, Audrey, Cho, Eun Jeong, Kaoud, Tamer S., Dalby, Kevin N.“…Oleic acid inhibits S-614G binding to ACE2 far more potently than to the receptor-binding domain (RBD, residues 319–541 of SARS-CoV-2 S), potentially indicating a noncompetitive mechanism. …”
Publicado 2022
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4617por Gomari, Mohammad Mahmoudi, Tarighi, Parastoo, Choupani, Edris, Abkhiz, Shadi, Mohamadzadeh, Masoud, Rostami, Neda, Sadroddiny, Esmaeil, Baammi, Soukayna, Uversky, Vladimir N., Dokholyan, Nikolay V.“…The B.1.617.2 lineage of SARS-CoV-2, being characterized by many Spike mutations inside and outside of its receptor-binding domain (RBD), shows high infectivity and relative resistance to existing cures. …”
Publicado 2023
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4618por Sertić, Zrinka, Lucijanić, Marko, Bašić-Kinda, Sandra, Serventi Seiwerth, Ranka, Periša, Vlatka, Sertić, Dubravka, Coha, Božena, Pulanić, Dražen, Perić, Zinaida, Desnica, Lana, Mikulić, Mirta, Vodanović, Marijo, Radman-Livaja, Ivo, Šegulja, Dragana, Rogić, Dunja, Valković, Toni, Aurer, Igor, Duraković, Nadira“…An electrochemiluminescent assay was used to measure total anti-SARS-CoV-2 S-RBD antibody titers. After one vaccine dose, 20/66 (33%) achieved seropositivity with a median antibody titer of 6.1 U/mL. …”
Publicado 2022
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4619por Zember, Sanja, Bodulić, Kristian, Balent, Nataša Cetinić, Mikulić, Radojka, Markotić, Alemka, Đaković Rode, Oktavija“…We analysed anti-SARS-CoV-2 Spike RBD IgG levels (positive ≥ 50 AU/mL) in 405 healthcare workers (3010 sera) who received a booster dose (BD) 9 months after two-dose BNT162b2 primovaccination. …”
Publicado 2022
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4620por Wu, You, Yang, Yi-Wen, Gu, Si-Chun, Zhang, Yu, Shi, Rong, Wang, Chang-De, Yuan, Can-Xing, Ye, Qing“…CONCLUSION: Specific nonmotor symptoms (RBD, gastrointestinal function and thermoregulation function) were associated with the PIGD subtype. …”
Publicado 2022
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