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4721por Yadav, Rohitash, Chaudhary, Jitendra Kumar, Jain, Neeraj, Chaudhary, Pankaj Kumar, Khanra, Supriya, Dhamija, Puneet, Sharma, Ambika, Kumar, Ashish, Handu, Shailendra“…Despite key amino acid residual variability, there is an incredible structural similarity between the receptor binding domain (RBD) of spike protein (S) of SARS-CoV-2 and SARS-CoV. …”
Publicado 2021
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4722por Klüpfel, Julia, Koros, Rosa Carolina, Dehne, Kerstin, Ungerer, Martin, Würstle, Silvia, Mautner, Josef, Feuerherd, Martin, Protzer, Ulrike, Hayden, Oliver, Elsner, Martin, Seidel, Michael“…As many commercial antibody tests are either time-consuming or tend to produce false negative or false positive results when only one antigen is considered, we developed an automated, flow-based chemiluminescence microarray immunoassay (CL-MIA) that allows for the detection of IgG antibodies to SARS-CoV-2 receptor-binding domain (RBD), spike protein (S1 fragment), and nucleocapsid protein (N) in human serum and plasma in less than 8 min. …”
Publicado 2021
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4723por Fu, Dan, Zhang, Guangshun, Wang, Yuhui, Zhang, Zheng, Hu, Hengrui, Shen, Shu, Wu, Jun, Li, Bo, Li, Xin, Fang, Yaohui, Liu, Jia, Wang, Qiao, Zhou, Yunjiao, Wang, Wei, Li, Yufeng, Lu, Zhonghua, Wang, Xiaoxiao, Nie, Cui, Tian, Yujie, Chen, Da, Wang, Yuan, Zhou, Xingdong, Wang, Qisheng, Yu, Feng, Zhang, Chen, Deng, Changjing, Zhou, Liang, Guan, Guangkuo, Shao, Na, Lou, Zhiyong, Deng, Fei, Zhang, Hongkai, Chen, Xinwen, Wang, Manli, Liu, Louis, Rao, Zihe, Guo, Yu“…From a large panel of isolated antibodies, 25 antibodies showed potent neutralizing activities at sub-nanomolar levels by engaging the spike receptor-binding domain (RBD). Importantly, one human NAb, termed PR1077, from the H2L2 platform and 2 humanized NAb, including PR953 and PR961, were further characterized and subjected for subsequent structural analysis. …”
Publicado 2021
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4724por Umashankar, V., Deshpande, Sanjay H., Hegde, Harsha V., Singh, Ishwar, Chattopadhyay, Debprasad“…Further, these compounds were virtually screened against Receptor Binding Domain (RBD) of Spike protein. The potential compounds from each plant were prioritized based on the binding affinity, key hotspot interactions at ACE2 binding region and glycosylation sites. …”
Publicado 2021
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4725por Dey, Ayan, Chozhavel Rajanathan, T.M., Chandra, Harish, Pericherla, Hari P.R., Kumar, Sanjeev, Choonia, Huzaifa S., Bajpai, Mayank, Singh, Arun K., Sinha, Anuradha, Saini, Gurwinder, Dalal, Parth, Vandriwala, Sarosh, Raheem, Mohammed A., Divate, Rupesh D., Navlani, Neelam L., Sharma, Vibhuti, Parikh, Aashini, Prasath, Siva, Sankar Rao, M., Maithal, Kapil“…The S protein of the virus includes the receptor binding domain (RBD), responsible for binding to the human angiotensin converting enzyme (ACE-2) receptor. …”
Publicado 2021
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4726por Luo, Shengxue, Zhang, Panli, Liu, Bochao, Yang, Chan, Liang, Chaolan, Wang, Qi, Zhang, Ling, Tang, Xi, Li, Jinfeng, Hou, Shuiping, Zeng, Jinfeng, Fu, Yongshui, Allain, Jean-Pierre, Li, Tingting, Zhang, Yuming, Li, Chengyao“…In macaques, prime-boost regimen induced high titers of 10(3.16) anti-S, 10(2.75) anti-RBD binding antibody and 10(2.38) pseudovirus neutralizing antibody (pNAb) at 2 months, while pNAb decreased gradually to 10(1.45) at 7 months post-priming. …”
Publicado 2021
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4727por Roxhed, Niclas, Bendes, Annika, Dale, Matilda, Mattsson, Cecilia, Hanke, Leo, Dodig-Crnković, Tea, Christian, Murray, Meineke, Birthe, Elsässer, Simon, Andréll, Juni, Havervall, Sebastian, Thålin, Charlotte, Eklund, Carina, Dillner, Joakim, Beck, Olof, Thomas, Cecilia E., McInerney, Gerald, Hong, Mun-Gwan, Murrell, Ben, Fredolini, Claudia, Schwenk, Jochen M.“…Thus, we establish a multianalyte and multiplexed approach to reliably profile IgG and IgM levels against several versions of SARS-CoV-2 proteins (S, RBD, N) in home-sampled dried blood spots (DBS). …”
Publicado 2021
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4728por Liu, Chang, Ginn, Helen M., Dejnirattisai, Wanwisa, Supasa, Piyada, Wang, Beibei, Tuekprakhon, Aekkachai, Nutalai, Rungtiwa, Zhou, Daming, Mentzer, Alexander J., Zhao, Yuguang, Duyvesteyn, Helen M.E., López-Camacho, César, Slon-Campos, Jose, Walter, Thomas S., Skelly, Donal, Johnson, Sile Ann, Ritter, Thomas G., Mason, Chris, Costa Clemens, Sue Ann, Gomes Naveca, Felipe, Nascimento, Valdinete, Nascimento, Fernanda, Fernandes da Costa, Cristiano, Resende, Paola Cristina, Pauvolid-Correa, Alex, Siqueira, Marilda M., Dold, Christina, Temperton, Nigel, Dong, Tao, Pollard, Andrew J., Knight, Julian C., Crook, Derrick, Lambe, Teresa, Clutterbuck, Elizabeth, Bibi, Sagida, Flaxman, Amy, Bittaye, Mustapha, Belij-Rammerstorfer, Sandra, Gilbert, Sarah C., Malik, Tariq, Carroll, Miles W., Klenerman, Paul, Barnes, Eleanor, Dunachie, Susanna J., Baillie, Vicky, Serafin, Natali, Ditse, Zanele, Da Silva, Kelly, Paterson, Neil G., Williams, Mark A., Hall, David R., Madhi, Shabir, Nunes, Marta C., Goulder, Philip, Fry, Elizabeth E., Mongkolsapaya, Juthathip, Ren, Jingshan, Stuart, David I., Screaton, Gavin R.“…Here we study the ability of monoclonal antibodies and convalescent and vaccine sera to neutralize B.1.617.1 and B.1.617.2, complement this with structural analyses of Fab/receptor binding domain (RBD) complexes, and map the antigenic space of current variants. …”
Publicado 2021
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4729por Vidal, Samuel J., Collier, Ai-ris Y., Yu, Jingyou, McMahan, Katherine, Tostanoski, Lisa H., Ventura, John D., Aid, Malika, Peter, Lauren, Jacob-Dolan, Catherine, Anioke, Tochi, Chang, Aiquan, Wan, Huahua, Aguayo, Ricardo, Ngo, Debby, Gerszten, Robert E., Seaman, Michael S., Barouch, Dan H.“…Correlates of cross-neutralization included receptor binding domain (RBD) and N-terminal domain (NTD) binding antibodies, homologous NAb titers, and membrane-directed T cell responses. …”
Publicado 2021
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4730por Shen, Lishuang, Bard, Jennifer Dien, Triche, Timothy J., Judkins, Alexander R., Biegel, Jaclyn A., Gai, Xiaowu“…It is defined by the N501Y mutation in the receptor-binding domain (RBD) of the Spike (S) protein, and a few other mutations. …”
Publicado 2021
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4731por Hoffmann, Donata, Corleis, Björn, Rauch, Susanne, Roth, Nicole, Mühe, Janine, Halwe, Nico Joel, Ulrich, Lorenz, Fricke, Charlie, Schön, Jacob, Kraft, Anna, Breithaupt, Angele, Wernike, Kerstin, Michelitsch, Anna, Sick, Franziska, Wylezich, Claudia, Hoffmann, Bernd, Thran, Moritz, Thess, Andreas, Mueller, Stefan O., Mettenleiter, Thomas C., Petsch, Benjamin, Dorhoi, Anca, Beer, Martin“…Naive mice and mice immunized with a formalin-inactivated SARS-CoV-2 preparation were used as controls. mRNA-immunized mice develop elevated SARS-CoV-2 RBD-specific antibody and neutralization titers which are readily detectable, but significantly reduced against VOC B.1.351. …”
Publicado 2021
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4732por Cosar, Begum, Karagulleoglu, Zeynep Yagmur, Unal, Sinan, Ince, Ahmet Turan, Uncuoglu, Dilruba Beyza, Tuncer, Gizem, Kilinc, Bugrahan Regaip, Ozkan, Yunus Emre, Ozkoc, Hikmet Ceyda, Demir, Ibrahim Naki, Eker, Ali, Karagoz, Feyzanur, Simsek, Said Yasin, Yasar, Bunyamin, Pala, Mehmetcan, Demir, Aysegul, Atak, Irem Naz, Mendi, Aysegul Hanife, Bengi, Vahdi Umut, Cengiz Seval, Guldane, Gunes Altuntas, Evrim, Kilic, Pelin, Demir-Dora, Devrim“…At the same time, it can be said that variations should occur in areas such as the receptor-binding domain (RBD), and vaccines and antiviral drugs should be formulated by targeting more than one viral protein. …”
Publicado 2022
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4733“…In addition, to demonstrate the applicability of the CFPS systems, it was proved that the four CFPS systems all had the potential to produce therapeutic proteins, and they could produce the receptor-binding domain (RBD) protein of SARS-CoV-2 with functional activity. …”
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4734“…Many derivatives had good binding affinity for the RBD of the of SARS-CoV-2 spike glycoprotein with the formation of up to four hydrogen bonds. …”
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4735por Momin, Taufik, Kansagra, Kevinkumar, Patel, Hardik, Sharma, Sunil, Sharma, Bhumika, Patel, Jatin, Mittal, Ravindra, Sanmukhani, Jayesh, Maithal, Kapil, Dey, Ayan, Chandra, Harish, Rajanathan, Chozhavel TM, Pericherla, Hari PR, Kumar, Pawan, Narkhede, Anjali, Parmar, Deven“…The spike(S) region includes the receptor-binding domain (RBD), which binds to the human angiotensin converting Enzyme (ACE)-2 receptor and mediates the entry of virus inside the cell. …”
Publicado 2021
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4736por Guo, Hua, Hu, Ben, Si, Hao-Rui, Zhu, Yan, Zhang, Wei, Li, Bei, Li, Ang, Geng, Rong, Lin, Hao-Feng, Yang, Xing-Lou, Zhou, Peng, Shi, Zheng-Li“…We found that the RaTG15 receptor-binding domain (RBD) can bind to ACE2 from Rhinolophus affinis, Malayan pangolin, and use it as an entry receptor, except for ACE2 from humans. …”
Publicado 2021
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4737por Bordoloi, Devivasha, Xu, Ziyang, Ho, Michelle, Purwar, Mansi, Bhojnagarwala, Pratik, Cassel, Joel, Giron, Leila B., Walker, Susanne, Kulkarni, Abhijeet J, Ruiz, Edgar Tello, Choi, Jihae, Zaidi, Faraz I., Wu, Yuanhan, Wang, Shaoying, Patel, Ami, Ramos, Stephanie, Smith, Trevor, Kulp, Daniel, Ugen, Kenneth E., Srinivasan, Alagarsamy, Abdel-Mohsen, Mohamed, Humeau, Laurent, Weiner, David B., Muthumani, Kar“…We identified a total of 10 antibodies, which specifically and strongly bound to SARS-CoV-2 Spike, blocked the receptor binding domain (RBD) and angiotensin-converting enzyme 2 (ACE2) interaction, and neutralized SARS-CoV-2. …”
Publicado 2021
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4738por Strengert, Monika, Becker, Matthias, Ramos, Gema Morillas, Dulovic, Alex, Gruber, Jens, Juengling, Jennifer, Lürken, Karsten, Beigel, Andrea, Wrenger, Eike, Lonnemann, Gerhard, Cossmann, Anne, Stankov, Metodi V., Dopfer-Jablonka, Alexandra, Kaiser, Philipp D., Traenkle, Bjoern, Rothbauer, Ulrich, Krause, Gérard, Schneiderhan-Marra, Nicole, Behrens, Georg M.N.“…We further compared binding capacity and neutralization efficacy of vaccination-induced immunoglobulins against emerging SARS-CoV-2 variants Alpha, Beta, Epsilon and Cluster 5 by ACE2-RBD competition assay. FINDINGS: Patients on maintenance haemodialysis exhibit detectable but variable cellular and humoral immune responses against SARS-CoV-2 and variants of concern after a two-dose regimen of BNT162b2. …”
Publicado 2021
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4739por Laiton-Donato, Katherine, Franco-Muñoz, Carlos, Álvarez-Díaz, Diego A., Ruiz-Moreno, Hector Alejandro, Usme-Ciro, José A., Prada, Diego Andrés, Reales-González, Jhonnatan, Corchuelo, Sheryll, Herrera-Sepúlveda, María T., Naizaque, Julian, Santamaría, Gerardo, Rivera, Jorge, Rojas, Paola, Ortiz, Juan Hernández, Cardona, Andrés, Malo, Diana, Prieto-Alvarado, Franklin, Gómez, Fernando Ruiz, Wiesner, Magdalena, Martínez, Martha Lucia Ospina, Mercado-Reyes, Marcela“…This strategy allowed us to identify the emergence of the B.1.621 lineage, considered a variant of interest (VOI) with the accumulation of several substitutions affecting the Spike protein, including the amino acid changes I95I, Y144T, Y145S and the insertion 146 N in the N-terminal domain, R346K, E484K and N501Y in the Receptor binding Domain (RBD) and P681H in the S1/S2 cleavage site of the Spike protein. …”
Publicado 2021
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4740por Makdasi, Efi, Zvi, Anat, Alcalay, Ron, Noy-Porat, Tal, Peretz, Eldar, Mechaly, Adva, Levy, Yinon, Epstein, Eyal, Chitlaru, Theodor, Tennenhouse, Ariel, Aftalion, Moshe, Gur, David, Paran, Nir, Tamir, Hadas, Zimhony, Oren, Weiss, Shay, Mandelboim, Michal, Mendelson, Ella, Zuckerman, Neta, Nemet, Ital, Kliker, Limor, Yitzhaki, Shmuel, Shapira, Shmuel C., Israely, Tomer, Fleishman, Sarel J., Mazor, Ohad, Rosenfeld, Ronit“…Four previously selected mAbs targeting non-overlapping epitopes are now evaluated for binding potency to mutated RBD versions, reported to mediate escape from antibody neutralization. …”
Publicado 2021
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