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4761por Zahn, Tobias, Mhedhbi, Ines, Hein, Sascha, Raupach, Jan, Miskey, Csaba, Husria, Younes, Bayanga, Kathrin, Bartel, Detlef, Vieths, Stefan, Ivics, Zoltan, Oberle, Doris, Keller‐Stanislawski, Brigitte, Herrlein, Marie‐Luise, Maier, Thorsten Jürgen, Hildt, Eberhard“…Characterization of the humoral immune response in comparison to patients that had fully recovered from mild COVID‐19 revealed a lack of antibodies binding to sequential epitopes of the receptor‐binding domain (RBD) for the long‐term infected patients. CONCLUSION: Thus, a small portion of COVID‐19 patients displays long‐term infectivity and termination of quarantine periods after 14 days, without PCR‐based testing, should be reconsidered critically.…”
Publicado 2021
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4762por Wendel, Silvano, Fachini, Roberta, Fontão‐Wendel, Rita C. L., Mello, Ralyria, Velasquez, Carlos Vinícius, Machado, Rafael Rahal Guaragna, Brito, Mayra Altobelli, Amaral, Marcelo, Soares, Camila Pereira, Achkar, Ruth, Scuracchio, Patrícia, Miyaji, Sandra Cristina, Erdens, Melina Santos, Durigon, Edison Luiz“…Samples were blindly tested for several surrogates: one anti‐RBD, two anti‐spike, and four anti‐nucleocapsid tests, either isolated or combined to improve their positive predictive values as predictors of the presence of high‐titer nAbs, defined as those with titers ≥160. …”
Publicado 2021
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4763por Cabral, Gabriela Bastos, Ahagon, Cintia Mayumi, de Souza Guimarães, Paula Morena, Silva Lopez-Lopes, Giselle Ibette, Hussein, Igor Mohamed, Cilli, Audrey, de Jesus Alves, Ivy, Coelho Bombonatte, Andréa Gobetti, do Carmo Sampaio Tavares Timenetsky, Maria, da Silva Santos, Jaqueline Helena, de Oliveira Santos, Katia Corrêa, dos Santos, Fabiana Cristina Pereira, de Macedo Brígido, Luís Fernando“…We describe Sanger based sequencing protocols: i) Semi-nested RT-PCR covering up to 3.684 kb (>96 %) spike gene; ii) One-Step RT-PCR for key Receptor Binding Domain (RBD) mutations (codons 417–501); iii) One-Step RT-PCR of partial N region to improve genomic capability. …”
Publicado 2022
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4764por Kim, Gyoung Nyoun, Choi, Jung-ah, Wu, Kunyu, Saeedian, Nasrin, Yang, Eunji, Park, Hayan, Woo, Sun-Je, Lim, Gippeum, Kim, Seong-Gyu, Eo, Su-Kyeong, Jeong, Hoe Won, Kim, Taewoo, Chang, Jae-Hyung, Seo, Sang Hwan, Kim, Na Hyung, Choi, Eunsil, Choo, Seungho, Lee, Sangkyun, Winterborn, Andrew, Li, Yue, Parham, Kate, Donovan, Justin M., Fenton, Brock, Dikeakos, Jimmy D., Dekaban, Gregory A., Haeryfar, S. M. Mansour, Troyer, Ryan M., Arts, Eric J., Barr, Stephen D., Song, Manki, Kang, C. Yong“…We have constructed VSV genomes carrying exogenous genes resulting in the production of avirulent rVSV carrying the full-length spike protein (S(F)), the S1 subunit, or the receptor-binding domain (RBD) plus envelope (E) protein of SARS-CoV-2. Adding the honeybee melittin signal peptide (msp) to the N-terminus enhanced the protein expression, and adding the VSV G protein transmembrane domain and the cytoplasmic tail (Gtc) enhanced protein incorporation into pseudotype VSV. …”
Publicado 2021
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4765por Peng, Lei, Hu, Yingxia, Mankowski, Madeleine C., Ren, Ping, Chen, Rita E., Wei, Jin, Zhao, Min, Li, Tongqing, Tripler, Therese, Ye, Lupeng, Chow, Ryan D., Fang, Zhenhao, Wu, Chunxiang, Dong, Matthew B., Cook, Matthew, Wang, Guilin, Clark, Paul, Nelson, Bryce, Klein, Daryl, Sutton, Richard, Diamond, Michael S., Wilen, Craig B., Xiong, Yong, Chen, Sidi“…By high-throughput single cell sequencing of B cells from spike receptor binding domain (RBD) immunized animals, we identified two highly potent SARS-CoV-2 neutralizing mAb clones that have single-digit nanomolar affinity and low-picomolar avidity, and generated a bispecific antibody. …”
Publicado 2021
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4766por Greco, Marilena, Cucci, Federico, Portulano, Pierfrancesco, Lazzari, Roberta Assunta, Caldararo, Cosimo, Sicuro, Fernando, Catanese, Carmelo, Lobreglio, Giambattista“…Anti-spike-receptor binding domain (RBD) level was tested 15 - 70 days after BNT162b2 second inoculum. …”
Publicado 2021
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4767por Marchan, Jose“…Remarkably, SARS-CoV-2 receptor-binding domain (RBD) and its receptor-binding motif (RBM) harbour several potential epitopes. …”
Publicado 2022
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4768“…It consists of Spike structures created from glycoproteins that promote the virus entry into the target cells of host. The RBD of the S1 subunit on Spike proteins binds to the hACE2 receptor, which is mostly found in the lungs, particularly type-2 pneumocytes, causing human ACE2 receptors to be downregulated. …”
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4769por Garrido, Carolina, Curtis, Alan D., Dennis, Maria, Pathak, Sachi H., Gao, Hongmei, Montefiori, David, Tomai, Mark, Fox, Christopher B., Kozlowski, Pamela A., Scobey, Trevor, Munt, Jennifer E., Mallory, Michael L., Saha, Pooja T., Hudgens, Michael G., Lindesmith, Lisa C., Baric, Ralph S., Abiona, Olubukola M., Graham, Barney S., Corbett, Kizzmekia S., Edwards, Darin, Carfi, Andrea, Fouda, Genevieve, Van Rompay, Koen K. A., De Paris, Kristina, Permar, Sallie R.“…Both vaccines elicited high-magnitude IgG binding to RBD, amino-terminal domain, S1 and S2, ACE2 blocking activity, and high neutralizing antibody titers, all peaking at week 6. …”
Publicado 2021
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4770“…SARS-CoV-2 is known to infect human through Spike glycoprotein and uses receptor-binding domain (RBD) to bind to the ACE2 receptor in human. Thus, it is of utmost importance to study these variants and their corresponding mutations. …”
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4771por Kalnin, Kirill V., Plitnik, Timothy, Kishko, Michael, Huang, Dean, Raillard, Alice, Piolat, Julie, Anosova, Natalie G., Tibbitts, Timothy, DiNapoli, Joshua, Karve, Shrirang, Goldman, Rebecca, Gopani, Hardip, Dias, Anusha, Tran, Khang, Zacharia, Minnie, Gu, Xiaobo, Boeglin, Lianne, Abysalh, Jonathan, Vargas, Jorel, Beaulieu, Angela, Shah, Monic, Jeannotte, Travis, Gillis, Kimberly, Chivukula, Sudha, Swearingen, Ron, Landolfi, Victoria, Fu, Tong-Ming, DeRosa, Frank, Casimiro, Danilo“…The data support the notion that a third vaccination is key to boosting existing titers and improving the breadth of antibodies to address variants of concern, including those with an E484K mutation in the Receptor Binding Domain (RBD) (Beta, Gamma).…”
Publicado 2022
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4772por Choteau, Mathilde, Scohy, Anaïs, Messe, Stéphane, Luyckx, Mathieu, Dechamps, Mélanie, Montiel, Virginie, Yombi, Jean Cyr, Gruson, Damien, Limaye, Nisha, Michiels, Thomas, Dumoutier, Laure“…We developed in-house ELISAs to measure titers of IgG, IgM and IgA directed against the RBD or N regions in patient serum or plasma, and a spike-pseudotyped neutralization assay to analyse seroneutralization. …”
Publicado 2022
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4773por Shehata, Awad A., Attia, Youssef A., Rahman, Md. Tanvir, Basiouni, Shereen, El-Seedi, Hesham R., Azhar, Esam I., Khafaga, Asmaa F., Hafez, Hafez M.“…The interplay between the receptor-binding domain (RBD) of coronaviruses and their coevolution are determinants for host susceptibility. …”
Publicado 2022
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4774Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoproteinpor Staufer, Oskar, Gupta, Kapil, Hernandez Bücher, Jochen Estebano, Kohler, Fabian, Sigl, Christian, Singh, Gunjita, Vasileiou, Kate, Yagüe Relimpio, Ana, Macher, Meline, Fabritz, Sebastian, Dietz, Hendrik, Cavalcanti Adam, Elisabetta Ada, Schaffitzel, Christiane, Ruggieri, Alessia, Platzman, Ilia, Berger, Imre, Spatz, Joachim P.“…This is achieved by regulation of the S open-to-close equilibrium and the exposure of both, the receptor binding domain (RBD) and the SARS-CoV-2 RGD motif that is responsible for integrin co-receptor engagement. …”
Publicado 2022
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4775por Cucunawangsih, Cucunawangsih, Wijaya, Ratna Sari, Lugito, Nata Pratama Hardjo, Suriapranata, Ivet“…OBJECTIVE: To evaluate the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein (anti-S) titers induced by the third mRNA-1273 vaccine among fully vaccinated HCWs with CoronaVac. …”
Publicado 2022
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4776por Cao, Kangli, Wang, Xiang, Peng, Haoran, Ding, Longfei, Wang, Xiangwei, Hu, Yangyang, Dong, Lanlan, Yang, Tianhan, Hong, Xiujing, Xing, Man, Li, Duoduo, Zhu, Cuisong, He, Xiangchuan, Zhao, Chen, Zhao, Ping, Zhou, Dongming, Zhang, Xiaoyan, Xu, Jianqing“…Our design is centered on an immunogen generated by fusing the SARS-CoV-2 receptor-binding domain (RBD) to the conserved stalk of H7N9 hemagglutinin (HA). …”
Publicado 2022
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4777por Chopra, Arvind, Chavan-Gautam, Preeti, Tillu, Girish, Saluja, Manjit, Borse, Swapnil, Sarmukaddam, Sanjeev, Chaudhuri, Susmita, Rao, BCS, Yadav, Babita, Srikanth, Narayanam, Patwardhan, Bhushan“…Primary outcome measure is immunogenicity as measured by SARS-CoV-2 spike (S1) and RBD-specific IgG antibody titres. Secondary outcome measures are safety, protective immune response and quality of life measures. …”
Publicado 2022
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4778por Medeiros, Giuliana X., Sasahara, Greyce Luri, Magawa, Jhosiene Y., Nunes, João Paulo S., Bruno, Fernanda R., Kuramoto, Andreia C., Almeida, Rafael R., Ferreira, Marcelo A., Scagion, Guilherme P., Candido, Érika D., Leal, Fabyano B., Oliveira, Danielle B. L., Durigon, Edison L., Silva, Roberto Carlos V., Rosa, Daniela S., Boscardin, Silvia B., Coelho, Verônica, Kalil, Jorge, Santos, Keity S., Cunha-Neto, Edecio“…We observed that among vaccinated individuals, men and individuals 55 years or older developed significantly lower anti-RBD, anti-NP and neutralization titers against the Wuhan strain and antigen-induced IL-2 production by T cells. …”
Publicado 2022
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4779por Wang, Shuang, Wang, Junchao, Yu, Xiaojuan, Jiang, Wen, Chen, Shuo, Wang, Rongjuan, Wang, Mingzhu, Jiao, Shasha, Yang, Yingying, Wang, Wenbo, Chen, Huilin, Chen, Ben, Gu, Chunying, Liu, Chuang, Wang, An, Wang, Min, Li, Gang, Guo, Cuicui, Liu, Datao, Zhang, Jinchao, Zhang, Min, Wang, Lan, Gui, Xun“…While, the neutralizing mAb MW07, which has no ADE activity only binds to RBD in S trimer with “up” state. Monovalent MW01 and MW05 completely diminished the ADE activity compared with their bivalent counterparts. …”
Publicado 2022
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4780por Wu, Xilin, Wang, Yaxing, Cheng, Lin, Ni, Fengfeng, Zhu, Linjing, Ma, Sen, Huang, Bilian, Ji, Mengmeng, Hu, Huimin, Li, Yuncheng, Xu, Shijie, Shi, Haixia, Zhang, Doudou, Liu, Linshuo, Nawaz, Waqas, Hu, Qinxue, Ye, Sheng, Liu, Yalan, Wu, Zhiwei“…Furthermore, the crystal structural analysis revealed that the binding of Nb22 to WH01 and Delta RBDs both effectively blocked the binding of RBD to hACE2. Additionally, intranasal Nb22 exhibited protection against SARS-CoV-2 Delta variant in the post-exposure prophylaxis (PEP) and pre-exposure prophylaxis (PrEP). …”
Publicado 2022
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