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461por Mani, Shailendra, Kaur, Anupamjeet, Jakhar, Kamini, Kumari, Geetika, Sonar, Sudipta, Kumar, Amit, Das, Sudesna, Kumar, Santosh, Kumar, Vijay, Kundu, Rakesh, Pandey, Anil Kumar, Singh, Umesh Prasad, Majumdar, Tanmay“…Here, we have demonstrated that the receptor binding domain (RBD) of the spike protein can interact with human dipeptidyl peptidase 4 (DPP4) to facilitate virus entry, in addition to the usual route of ACE2-RBD binding. …”
Publicado 2023
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462por Akahata, Wataru, Sekida, Takashi, Nogimori, Takuto, Ode, Hirotaka, Tamura, Tomokazu, Kono, Kaoru, Kazami, Yoko, Washizaki, Ayaka, Masuta, Yuji, Suzuki, Rigel, Matsuda, Kenta, Komori, Mai, Morey, Amber L., Ishimoto, Keiko, Nakata, Misako, Hasunuma, Tomoko, Fukuhara, Takasuke, Iwatani, Yasumasa, Yamamoto, Takuya, Smith, Jonathan F., Sato, Nobuaki“…VLPCOV-01 is a lipid nanoparticle-encapsulated self-amplifying RNA (saRNA) vaccine that expresses a membrane-anchored receptor-binding domain (RBD) derived from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. …”
Publicado 2023
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463por Takahashi, Etsuhisa, Sawabuchi, Takako, Homma, Tetsuya, Fukuda, Yosuke, Sagara, Hironori, Kinjo, Takeshi, Fujita, Kaori, Suga, Shigeru, Kimoto, Takashi, Sakai, Satoko, Kameda, Keiko, Kido, Hiroshi“…Here, we quantified anti-SARS-CoV-2 antibody binding avidity to the receptor-binding domain (RBD) by competitive binding-inhibition activity (IC50) between SARS-CoV-2 S1 antigen immobilized on the DCP microarray and various RBD doses added to serum and expressed as 1/IC50 nM. …”
Publicado 2023
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464por Zhang, Yanfang, Kang, Xinrui, Liu, Sheng, Han, Pu, Lei, Wenwen, Xu, Ke, Xu, Zepeng, Gao, Zhengrong, Zhou, Xuemei, An, Yaling, Han, Yuxuan, Liu, Kefang, Zhao, Xin, Dai, Lianpan, Wang, Peiyi, Wu, Guizhen, Qi, Jianxun, Xu, Kun, Gao, George F.“…In this study, we further explored the concept of tandem RBD homotrimer and heterotrimer. Prototype SARS-CoV-2 RBD-homotrimer, prototype-Delta-BA.1 (PDO) RBD-heterotrimer and Delta-BA.2-BA.5 (DBA2BA5) RBD-heterotrimer were designed. …”
Publicado 2023
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465por Wetzel, Christoph, Jansen-Olliges, Linda, Stadler, Marc, Surup, Frank, Zeilinger, Carsten, Roth, Bernhard“…The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. …”
Publicado 2023
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466por Kuczkowska, Katarzyna, Bjerkan, Louise, Stubsrud, Elisabeth, Husbyn, Hannah Cuthbertson, Chellappa, Stalin, Hauge, Anette, Skarshaug, Renate, Torgersen, Maria Lyngaas, Heim, Joel Benjamin, Jørgensen, Marthe Jøntvedt, Wold, Christian Winther, Schleimann, Mariane Høgsbjerg, Tolstrup, Martin, Granum, Stine, Fredriksen, Agnete Brunsvik, Pedersen, Mikkel Wandahl, Norheim, Gunnstein“…Immunogenicity studies in mice demonstrated that the APC-targeted vaccine induced strong antibody responses to both homologous Beta RBD and heterologous RBDs derived from Wuhan, Alpha, Gamma, Delta, and Omicron BA.1 variants, as well as cross-neutralizing antibodies against these VoC. …”
Publicado 2023
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468por Muhseen, Ziyad Tariq, Hameed, Alaa R., Al-Hasani, Halah M.H., Tahir ul Qamar, Muhammad, Li, Guanglin“…The spike protein receptor binding domain (S-RBD) is a necessary corona-viral protein for binding and entry of coronaviruses (COVs) into the host cells. …”
Publicado 2020
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469“…Activity guided isolation of constituents was carried out by measuring the inhibitory activity on ACE2-RBD interaction. The structures of active compounds were identified by HPLC-Q-TOF-MS/MS and NMR. …”
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470por Manček‐Keber, Mateja, Hafner‐Bratkovič, Iva, Lainšček, Duško, Benčina, Mojca, Govednik, Tea, Orehek, Sara, Plaper, Tjaša, Jazbec, Vid, Bergant, Valter, Grass, Vincent, Pichlmair, Andreas, Jerala, Roman“…Here, we demonstrate that the integrity of disulfide bonds within the receptor‐binding domain (RBD) plays an important role in the membrane fusion process although their disruption does not prevent binding of spike protein to ACE2. …”
Publicado 2021
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471por Chen, Yuxin, Zhu, Liguo, Huang, Weijin, Tong, Xin, Wu, Hai, Tao, Yue, Tong, Bei, Huang, Haibin, Chen, Jiachen, Zhao, Xiangan, Lou, Yang, Wu, Chao“…Four vaccine-elicited rabbit monoclonal antibodies (RmAbs), including 1H1, 9H1, 7G5, and 5E1, were isolated for biophysical property, neutralization potency and sequence analysis. All RmAbs recognized RBD or S1 protein with K(D) in the low nM or sub nM range. 1H1 and 9H1, but neither 7G5 nor 5E1, can bind to all RBD protein variants derived from B.1.351. …”
Publicado 2021
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472por Rahnama, Shadi, Azimzadeh Irani, Maryam, Amininasab, Mehriar, Ejtehadi, Mohammad Reza“…The increase of RBD binding affinity to ACE2 depends on the size of attached O-glycan. …”
Publicado 2021
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473por Tandhavanant, Sarunporn, Koosakunirand, Sirikamon, Kaewarpai, Taniya, Piyaphanee, Watcharapong, Leaungwutiwong, Pornsawan, Luvira, Viravarn, Chantratita, Narisara“…Both RBD-specific IgA and IgG were detected in only 21% of the COVID-19 patients in the acute phase. …”
Publicado 2021
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474por Rattanapisit, Kaewta, Bulaon, Christine Joy I., Khorattanakulchai, Narach, Shanmugaraj, Balamurugan, Wangkanont, Kittikhun, Phoolcharoen, Waranyoo“…In order to study the effect of mutation in the binding efficiency of SARS-CoV-2 receptor binding domain (RBD) with anti-SARS-CoV/CoV-2 monoclonal antibodies (mAbs), we have produced SARS-CoV-2 RBD and two variants SARS-CoV-2 RBD (Alpha RBD and Beta RBD) in Nicotiana benthamiana by transient expression. …”
Publicado 2021
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475por Zang, Jinkai, Zhu, Yuanfei, Zhou, Yu, Gu, Chenjian, Yi, Yufang, Wang, Shuxia, Xu, Shiqi, Hu, Gaowei, Du, Shujuan, Yin, Yannan, Wang, Yalei, Yang, Yong, Zhang, Xueyang, Wang, Haikun, Yin, Feifei, Zhang, Chao, Deng, Qiang, Xie, Youhua, Huang, Zhong“…We further designed and expressed a genetically linked dimeric RBD protein in yeast. The engineered dimeric RBD was more potent than the monomeric RBD in inducing long-lasting neutralizing antibodies. …”
Publicado 2021
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476por Meng, Xianmin, Wang, Peipei, Xiong, Yanqing, Wu, Yijun, Lin, Xiaoyan, Lu, Song, Li, Ruowan, Zhao, Bei, Liu, Jing, Zeng, Shaoqing, Zeng, Liyan, Wu, Yan, Lu, Yan, Zhang, Jinchao, Liu, Datao, Wang, Shuhai, Lu, HongzhouEnlace del recurso
Publicado 2021
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477por Sun, Wenqiang, He, Lihong, Zhang, He, Tian, Xiaodong, Bai, Zhihua, Sun, Lei, Yang, Limin, Jia, Xiaojuan, Bi, Yuhai, Luo, Tingrong, Cheng, Gong, Fan, Wenhui, Liu, Wenjun, Li, Jing“…Compared to the trimeric form of the RBD mRNA vaccine (T-RBD), TF-RBD delivered intramuscularly elicited robust and durable humoral immunity as well as a Th1-biased cellular response. …”
Publicado 2021
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478por Boulton, Stephen, Poutou, Joanna, Martin, Nikolas T., Azad, Taha, Singaravelu, Ragunath, Crupi, Mathieu J.F., Jamieson, Taylor, He, Xiaohong, Marius, Ricardo, Petryk, Julia, Tanese de Souza, Christiano, Austin, Bradley, Taha, Zaid, Whelan, Jack, Khan, Sarwat T., Pelin, Adrian, Rezaei, Reza, Surendran, Abera, Tucker, Sarah, Brown, Emily E.F., Dave, Jaahnavi, Diallo, Jean-Simon, Auer, Rebecca, Angel, Jonathan B., Cameron, D. William, Cailhier, Jean-Francois, Lapointe, Réjean, Potts, Kyle, Mahoney, Douglas J., Bell, John C., Ilkow, Carolina S.“…To combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), we characterize a temperature-stable vaccine candidate (TOH-Vac1) that uses a replication-competent, attenuated vaccinia virus as a vector to express a membrane-tethered spike receptor binding domain (RBD) antigen. We evaluate the effects of dose escalation and administration routes on vaccine safety, efficacy, and immunogenicity in animal models. …”
Publicado 2022
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479por Chen, Wen-Hsiang, Pollet, Jeroen, Strych, Ulrich, Lee, Jungsoon, Liu, Zhuyun, Kundu, Rakhi Tyagi, Versteeg, Leroy, Villar, Maria Jose, Adhikari, Rakesh, Wei, Junfei, Poveda, Cristina, Keegan, Brian, Bailey, Aaron Oakley, Chen, Yi-Lin, Gillespie, Portia M., Kimata, Jason T., Zhan, Bin, Hotez, Peter J., Bottazzi, Maria Elena“…The RBD203-N1 antigen was expressed in the yeast Pichia pastoris X33. …”
Publicado 2022
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480por GÜLER, Halil İbrahim, AY ŞAL, Fulya, CAN, Zehra, KARA, Yakup, YILDIZ, Oktay, BELDÜZ, Ali Osman, ÇANAKÇI, Sabriye, KOLAYLI, SevgiEnlace del recurso
Publicado 2021
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