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4801por Fernandes-Siqueira, Lorena O., Ferreira, Fabiana A. P., Sousa, Bruna G., Mebus-Antunes, Nathane C., Neves-Martins, Thais C., Almeida, Fabio C. L., Ferreira, Gustavo C., Salmon, Didier, Wermelinger, Luciana S., Da Poian, Andrea T.“…Here we present optimizations of all steps of an enzyme-linked immunosorbent assay (ELISA)-based test to detect IgG, IgA and IgM against the trimeric spike (S) protein, receptor binding domain (RBD), and N terminal domain of the nucleocapsid (N-NTD) protein of SARS-CoV-2. …”
Publicado 2022
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4802por Favalli, Andrea, Favalli, Ennio Giulio, Gobbini, Andrea, Zagato, Elena, Bombaci, Mauro, Maioli, Gabriella, Pesce, Elisa, Donnici, Lorena, Gruarin, Paola, Biggioggero, Martina, Curti, Serena, Manganaro, Lara, Marchisio, Edoardo, Bevilacqua, Valeria, Martinovic, Martina, Fabbris, Tanya, Sarnicola, Maria Lucia, Crosti, Mariacristina, Marongiu, Laura, Granucci, Francesca, Notarbartolo, Samuele, Bandera, Alessandra, Gori, Andrea, De Francesco, Raffaele, Abrignani, Sergio, Caporali, Roberto, Grifantini, Renata“…To provide an integrated analysis of the immune response following SARS-CoV-2 infection in RMD patients treated with different classes of DMARDs we carried out an immunological analysis of the antibody responses toward SARS-CoV-2 nucleocapsid and RBD proteins and an extensive immunophenotypic analysis of the major immune cell populations. …”
Publicado 2022
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4803por Moya, Martin, Marrama, Marcela, Dorazio, Carina, Veigas, Florencia, Manselle Cocco, Montana N., Dalotto Moreno, Tomas, Rabinovich, Gabriel A., Aleksandroff, Ariel“…Methods: A phase 3, open-label clinical trial involving administration of two adenoviral vectors (Ad26-Ad5) vaccine, in elderly individuals over the ages of 60 years was performed. SARS-CoV-2 Spike RBD-specific IgG antibodies at days 21-, 42- and 180 post-vaccination was analyzed in sera of individuals receiving two doses of the Sputnik-V vaccine with an interval of 21 days. …”
Publicado 2022
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4804por Gelanew, Tesfaye, Mulu, Andargachew, Abebe, Markos, Bates, Timothy A., Wassie, Liya, Teferi, Mekonnen, Fentahun, Dessalegn, Alemu, Aynalem, Tamiru, Frehiwot, Assefa, Gebeyehu, Bayih, Abebe Genetu, Tafesse, Fikadu G., Mihret, Adane, Abdissa, Alemseged“…We compared the geometric mean titers (GMTs) of the SARS-CoV-2 receptor-binding domain (RBD)-specific IgG antibodies in 39 SARS-CoV-2 naïve participants and 24 participants previously infected with SARS-CoV-2 (P.I.), who received two doses of ChAdOx1 nCoV-19 vaccine across the two post-vaccination time points (at 8 to 12 weeks post single dose and two dose vaccinations). …”
Publicado 2022
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4805por Azali, Muhammad Azharuddin, Mohamed, Salmah, Harun, Azian, Hussain, Faezahtul Arbaeyah, Shamsuddin, Shaharum, Johan, Muhammad Farid“…It is available in its whole length or as subunits like S1 or the receptor-binding domain (RBD). Non-structural proteins (Nsps), another recombinant SARS-CoV-2 protein generated by the baculovirus expression vector system (BEV), are used in the identification of new medications or the repurposing of existing therapies for the treatment of COVID-19. …”
Publicado 2022
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4806por Nikitin, Pavel A., DiMuzio, Jillian M., Dowling, John P., Patel, Nirja B., Bingaman-Steele, Jamie L., Heimbach, Baron C., Henriquez, Noeleya, Nicolescu, Chris, Polley, Antonio, Sikorski, Eden L., Howanski, Raymond J., Nath, Mitchell, Shukla, Halley, Scheaffer, Suzanne M., Finn, James P., Liang, Li-Fang, Smith, Todd, Storm, Nadia, McKay, Lindsay G. A., Johnson, Rebecca I., Malsick, Lauren E., Honko, Anna N., Griffiths, Anthony, Diamond, Michael S., Sarma, Purnanand, Geising, Dennis H., Morin, Michael J., Robinson, Matthew K.“…Binding of the third antibody, IMM20253, to its cryptic epitope on the outer surface of RBD, altered the conformation of the Spike Trimer, promoting release of Spike monomers. …”
Publicado 2022
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4807por Ban, Deependra Kumar, Bodily, Tyler, Karkisaval, Abhijith G., Dong, Yongliang, Natani, Shreyam, Ramanathan, Anirudh, Ramil, Armando, Srivastava, Sunil, Bandaru, Prab, Glinsky, Gennadi, Lal, Ratnesh“…We have developed a DNA aptamer-conjugated graphene field-effect transistor (GFET) biosensor platform to detect receptor-binding domain (RBD), nucleocapsid (N), and spike (S) proteins, as well as viral particles of original Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus and its variants in saliva samples. …”
Publicado 2022
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4808por Fibke, Chad D., Joffres, Yayuk, Tyson, John R., Colijn, Caroline, Janjua, Naveed Z., Fjell, Chris, Prystajecky, Natalie, Jassem, Agatha, Sbihi, Hind“…The identified mutations cover multiple spike domains including the N-terminal domain (NTD), receptor binding domain (RBD), S1/S2 cleavage region, fusion peptide and heptad regions. …”
Publicado 2022
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4809por Jiang, Wen, Zhang, Zherui, Zhu, Yuhe, Chen, Ben, Gu, Chunying, Liu, Zhiyan, Zhang, Xukai, Xiong, Hualong, Zhang, Yanan, Zheng, Bin, Wang, Rongjuan, Jiao, Shasha, Wang, An, Zhang, Tianying, Zhang, Jinchao, Wang, Shuang, Zhang, Bo, Li, Gang, Gui, Xun“…In this work, we comprehensively characterize the breadth and efficacy of SARS-CoV-2 RBD-targeting fully human monoclonal antibody (mAb) MW3321. …”
Publicado 2022
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4810por Ye, Qing, Wu, Mei, Zhou, Chao, Lu, Xishan, Huang, Baoying, Zhang, Ning, Zhao, Hui, Chi, Hang, Zhang, Xiaojing, Ling, Dandan, Zhang, Rong-Rong, Li, Zhuofan, Luo, Dan, Huang, Yi-Jiao, Qiu, Hong-Ying, Song, Haifeng, Tan, Wenjie, Xu, Ke, Ying, Bo, Qin, Cheng-Feng“…Then, ARIAV was combined with our COVID-19 mRNA vaccine ARCoV, which encodes the receptor-binding domain (RBD) of the SARS-CoV-2 S protein, to formulate the final combined vaccine, AR-CoV/IAV. …”
Publicado 2022
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4811por Li, Tong, Song, Rui, Wang, Jingjie, Zhang, Jianbo, Cai, Hongxing, He, Hongmei, Hu, Wei, Yu, Dajun, Wang, Chuanhu, Pan, Qingbo, Peng, Mingli, Ren, Hong, Zhu, Peng“…The data regarding adverse effects, serum anti-receptor binding domain (RBD)-IgG, neutralizing antibodies (NAbs), and frequencies of MBCs were collected prospectively. …”
Publicado 2022
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4812“…MD simulation revealed that point mutations in spike glycoprotein and RBD:ACE-2 complex cause changes in protein dynamics compared to the wild-type, suggesting possible alterations in binding affinity. …”
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4813por Peng, Mian, Dou, Xiaowen, Zhang, Xiuming, Yan, Mingchen, Xiong, Dan, Jiang, Ruiwei, Ou, Tong, Tang, Aifa, Yu, Xiqiu, Zhu, Feiqi, Li, Weiqin“…The amino acids with the highest scores in the short-term samples were distributed primarily in the receptor binding domain (RBD) and N-terminal domain regions of spike (S) protein, while in the long-term samples (12 weeks after the 2(nd) dose), some new conformational epitopes (CEs) were localized to crevices within the head of the S protein trimer. …”
Publicado 2022
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4814por Ferrari, Ludovica, Compagno, Mirko, Campogiani, Laura, Teti, Elisabetta, Mulas, Tiziana, Checchi, Davide, Alessio, Grazia, Caldara, Federica, Coppola, Luigi, De Simone, Giuseppe, Ceccarelli, Laura, Spalliera, Ilaria, Vitale, Pietro, Grelli, Sandro, Andreoni, Massimo, Sarmati, Loredana, Iannetta, Marco“…Conclusions: vaccine-related side effects and a higher anti-SARS-CoV-2-RBD IgG titer were more common in subjects with previous infection than in SARS-CoV-2-naïve after the first, but not after the second dose of the BNT162b2 vaccine.…”
Publicado 2022
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4815por Glowinska, Irena, Labij-Reduta, Barbara, Juzwiuk, Jerzy, Lukaszewicz, Magdalena, Pietruczuk, Adam, Poplawska, Agata, Daniluk-Jamro, Anna, Kakareko, Katarzyna, Rydzewska-Rosolowska, Alicja, Naumnik, Beata, Koc-Zorawska, Ewa, Zorawski, Marcin, Hryszko, Tomasz“…Anti-spike protein receptor-binding domain antibodies (anti-S IgG) were assessed using SARS-CoV-2 (RBD) IgG ELISA EIA-6150 IVD assay at baseline, on the 21st day and 43rd day, before a booster dose and two weeks thereafter. …”
Publicado 2022
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4816por Cao, Tianyu, Liu, Li, To, Kelvin Kai‐Wang, Lim, Chun‐Yu, Zhou, Runhong, Ming, Yue, Kwan, Ka‐Yi, Yu, Sulan, Chan, Chun‐Yin, Zhou, Biao, Huang, Haode, Mo, Yufei, Du, Zhenglong, Gong, Ruomei, Yat, Luk‐Tsz, Hung, Ivan Fan‐Ngai, Tam, Anthony Raymond, To, Wing‐Kin, Leung, Wai‐Shing, Chik, Thomas Shiu‐Hong, Tsang, Owen Tak‐Yin, Lin, Xiang, Song, You‐qiang, Yuen, Kwok‐Yung, Chen, Zhiwei“…Plasma samples were tested for cytokine concentration, and titer of Nabs, RBD‐, S1‐, SSA/Ro‐ and dsDNA‐specific IgG. RESULTS: While critically ill patients displayed predominantly extrafollicular B cell activation with elevated inflammation, mild patients counteracted the disease through the timely induction of mitochondrial dysfunction in B cells within the first week post symptom onset. …”
Publicado 2022
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4817por Srichana, Teerapol, Chunhachaichana, Charisopon, Suedee, Roongnapa, Sawatdee, Somchai, Changsan, Narumon“…CBD-MDI effectively reduced the generation of cytokines in immune cells treated with viral antigen S-RBD, bacterial antigen LPS, silica particles, and coal tar. …”
Publicado 2022
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4818por Ku, Zhiqiang, Xie, Xuping, Lin, Jianqing, Gao, Peng, Wu, Bin, El Sahili, Abbas, Su, Hang, Liu, Yang, Ye, Xiaohua, Tan, Eddie Yongjun, Li, Xin, Fan, Xuejun, Goh, Boon Chong, Xiong, Wei, Boyd, Hannah, Muruato, Antonio E., Deng, Hui, Xia, Hongjie, Zou, Jing, Kalveram, Birte K., Menachery, Vineet D., Zhang, Ningyan, Lescar, Julien, Shi, Pei-Yong, An, Zhiqiang“…Single molecules of both bsAbs block the two epitopes targeted by parental antibodies on the receptor-binding domain (RBD). However, bsAb with the IgG-(scFv)(2) design (14-H-06) but not the CrossMAb design (14-crs-06) shows increased antigen-binding and virus-neutralizing activities against multiple SARS-CoV-2 variants as well as increased breadth of neutralizing activity compared to the cocktail. …”
Publicado 2022
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4819por Li, Qi, Humphries, Fiachra, Girardin, Roxie C., Wallace, Aaron, Ejemel, Monir, Amcheslavsky, Alla, McMahon, Conor T., Schiller, Zachary A., Ma, Zepei, Cruz, John, Dupuis, Alan P., Payne, Anne F., Maryam, Arooma, Yilmaz, Nese Kurt, McDonough, Kathleen A., Pierce, Brian G., Schiffer, Celia A., Kruse, Andrew C., Klempner, Mark S., Cavacini, Lisa A., Fitzgerald, Katherine A., Wang, Yang“…VHH-IgA1.1 recognizes a conserved epitope of SARS-CoV-2 spike protein Receptor Binding Domain (RBD) and potently neutralizes major global SARS-CoV-2 variants of concern (VOC) including the Omicron variant and its sub lineages BA.1.1, BA.2 and BA.2.12.1. …”
Publicado 2022
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4820por Gan, Jinghuan, Liu, Shuai, Wang, Fei, Shi, Zhihong, Lü, Yang, Niu, Jianping, Meng, Xinling, Cai, Pan, Wang, Xiao-Dan, Chen, Zhichao, Gang, Baozhi, Ji, Yong“…Of the 101 participants, all of them (100.0%) had cognitive impairment, 46 (44.54%) displayed fluctuating cognition, 72 (71.29%) of them showed visual hallucination, 22 (21.78%) individuals reported RBD, and 27.71% showed Parkinsonism. Sleeping for <5 h (adjusted OR = 1.795, 95%CI: 1.055–3.054, p < 0.05) or having hypersomnolence (adjusted OR = 31.213, 95% CI: 17.618–55.301, p < 0.001) were significantly associated with the occurrence of DLB. …”
Publicado 2022
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