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4841por Baker, Forrest L., Zúñiga, Tiffany M., Smith, Kyle A., Batatinha, Helena, Kulangara, Terese S., Seckeler, Michael D., Burgess, Shane C., Katsanis, Emmanuel, Simpson, Richard J.“…Here we determine the antigen-specific CD4 and CD8 T-cell and humoral immunity to exercise in non-vaccinated individuals with natural immunity to SARS CoV-2, using whole-blood SARS-CoV-2 peptide stimulation assays, IFN-γ ELISPOT assays, 8-color flow cytometry, deep T-cell receptor (TCR) β sequencing, and anti-RBD-1 neutralizing antibody serology. We found that acute exercise reliably mobilized (∼2.5-fold increase) highly functional SARS-CoV-2-specific T-cells to the blood compartment in those with natural immunity to the virus. …”
Publicado 2023
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4842por Quartuccio, Luca, De Marchi, Ginevra, Domenis, Rossana, Cabas, Nicola, Guella, Silvia, Paradiso, Antonella, Fabro, Cinzia, Beltrami, Antonio Paolo, De Vita, Salvatore, Curcio, Francesco“…Comparison with the control group was performed at time T0 (i.e., 4 weeks after the second dose). Total anti-SARS-CoV-2 RBD antibodies were analyzed using a diagnostic assay, while cellular-mediated response was evaluated using the interferon-gamma release assay (IGRA). …”
Publicado 2023
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4843“…Prospective or retrospective studies comparing responders with non-responders against SARS-CoV-2 spike (S) protein receptor-binding domain (RBD) following COVID-19 vaccination were included. …”
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4844por Jacob-Dolan, Catherine, Yu, Jingyou, McMahan, Katherine, Giffin, Victoria, Chandrashekar, Abishek, Martinot, Amanda J., Anioke, Tochi, Powers, Olivia C., Hall, Kevin, Hope, David, Miller, Jessica, Hachmann, Nichole P., Chung, Benjamin, Gardner, Sarah, Sellers, Daniel, Barrett, Julia, Lewis, Mark G., Andersen, Hanne, Kleanthous, Harry, Seo, Ki-Woen, Lee, Su Jeen, Park, Yong Wook, Kim, Hun, Barouch, Dan H.“…The antigen in GBP510/AS03 is a two-part recombinant nanoparticle, which displays 60 receptor binding domain (RBD) proteins of SARS-CoV-2 Spike on its surface. …”
Publicado 2023
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4845por Dodaran, Masoud Solaymani, Banihashemi, Seyed Reza, Es-haghi, Ali, Mehrabadi, Mohammad Hossein Fallah, Nofeli, Mojtaba, Mokarram, Ali Rezaei, Mokhberalsafa, Ladan, Sadeghi, Fariba, Ranjbar, Alireza, Ansarifar, Akram, Mohazzab, Arash, Setarehdan, Seyed Amin, Bagheri Amiri, Fahimeh, Mohseni, Vahideh, Hajimoradi, Monireh, Ghahremanzadeh, Neda, Razzaz, Seyed Hossein, Masoomi, Safdar, Taghdiri, Maryam, Bagheri, Mohsen, Lofti, Mohsen, Khorasani, Akbar, Ghader, Masoud, Safari, Shiva, Shahsavn, Masumeh, Kalantari, Saeed“…The geometric mean ratios (GMRs) and 95% CI for serum neutralizing antibodies compared with placebo two weeks after the second injection were 5.82 (1.46–23.13), 11.12 (2.74–45.09), and 20.70 (5.05–84.76) in 5, 10, and 20 µg vaccine groups, respectively. The GMR for anti-RBD IgA in mucosal fluid two weeks after the intranasal dose was 23.27 (21.27–25.45) in the 10 µg vaccine group. …”
Publicado 2023
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4846por Hasibuan, Anshari Saifuddin, Koesnoe, Sukamto, Widhani, Alvina, Muhadi, Muhadi, Shatri, Hamzah, Ginanjar, Eka, Yunihastuti, Evy, Soewondo, Pradana, Aman Nasution, Sally, Djauzi, Samsuridjal, Dina Liastuti, Lies, Koento, Trimartani, Sumariyono, Sumariyono, Mulyantini, Astri“…Blood samples were taken to assess IgG sRBD antibody levels. The median antibody level was found to be 659 BAU/mL (min 37 BAU/mL, max 5680 BAU/mL, QIR 822 BAU/mL) after the booster, and this was not related to age, sex, comorbidities, or adverse events following immunization (AEFI) after the booster. …”
Publicado 2023
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4847por Bianchini, Filippo, Crivelli, Virginia, Abernathy, Morgan E., Guerra, Concetta, Palus, Martin, Muri, Jonathan, Marcotte, Harold, Piralla, Antonio, Pedotti, Mattia, De Gasparo, Raoul, Simonelli, Luca, Matkovic, Milos, Toscano, Chiara, Biggiogero, Maira, Calvaruso, Veronica, Svoboda, Pavel, Cervantes Rincón, Tomás, Fava, Tommaso, Podešvová, Lucie, Shanbhag, Akanksha A., Celoria, Andrea, Sgrignani, Jacopo, Stefanik, Michal, Hönig, Vaclav, Pranclova, Veronika, Michalcikova, Tereza, Prochazka, Jan, Guerrini, Giuditta, Mehn, Dora, Ciabattini, Annalisa, Abolhassani, Hassan, Jarrossay, David, Uguccioni, Mariagrazia, Medaglini, Donata, Pan-Hammarström, Qiang, Calzolai, Luigi, Fernandez, Daniel, Baldanti, Fausto, Franzetti-Pellanda, Alessandra, Garzoni, Christian, Sedlacek, Radislav, Ruzek, Daniel, Varani, Luca, Cavalli, Andrea, Barnes, Christopher O., Robbiani, Davide F.“…Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization and, like fp.006 and hr2.016, protects mice expressing human ACE2 against infection when present as bispecific antibody. …”
Publicado 2023
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4848por Li, Entao, Han, Qiuxue, Bi, Jinhao, Wei, Shimeng, Wang, Shen, Zhang, Ying, Liu, Jun, Feng, Na, Wang, Tiecheng, Wu, Jun, Yang, Songtao, Zhao, Yongkun, Liu, Bo, Yan, Feihu, Xia, Xianzhu“…In our study, polyclonal antibodies and F(ab’)(2) fragments with strong affinity produced after equine immunization with RBD proteins produced strong affinity. Notably, specific equine IgG and F(ab’)(2) have broad and high neutralizing activity against parental virus, all SARS-CoV-2 variants of concern (VOCs), including B.1.1,7, B.1.351, B.1.617.2, P.1, B.1.1.529 and BA.2, and all variants of interest (VOIs) including B.1.429, P.2, B.1.525, P.3, B.1.526, B.1.617.1, C.37 and B.1.621. …”
Publicado 2023
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4849por Braun, Molly R., Martinez, Clarissa I., Dora, Emery G., Showalter, Laura J., Mercedes, Annette R., Tucker, Sean N.“…Both intranasal and oral administration of rAd5-S-Wuhan generated cross-reactive serum IgG and mucosal IgA to all variant spike and RBD proteins tested. rAd5-S-omicron and rAd5-S-delta additionally elicited cross-reactive antibodies, though rAd5-S-omicron had significantly lower binding antibody levels except against its matched antigens. …”
Publicado 2023
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4850“…The FM′M-UD promoter increased protein levels of reporters GFP, RBD : SD1 (part of S protein from SARS-CoV-2), and human interleukin-6 (hIL6) by 4–6-fold, 2-fold, and 6-fold, respectively, relative to those of the same reporters driven by the CaMV 35S promoter. …”
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4851por Chatwani, Bhoomija, Flaherty, Shelby, Liu, Sharon, Theberge, Marc, Zeller, Mark, Anderson, Kristian, Boisen, Matt, Branco, Luis, Garry, Robert, Drouin, Arnaud, Fusco, Dahlene“…Convalescent antibodies (ReSARS N IgG, S-RBD IgG) were measured and percentage inhibition of ACE2 spike interaction was recorded. …”
Publicado 2021
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4852por Ollila, Thomas A., Masel, Rebecca, Reagan, John L, Paiva, Kimberly, Lu, Shaolei, Rogers, Ralph, Zayac, Adam, Taher, Rashida, Yakirevich, Inna, Niroula, Rabin, Barth, Peter, Olszewski, Adam J.“…Seroconversion was assessed by the qualitative SARS-CoV-2 Total Antibody Test (IgG/IgM against Receptor Binding Domain [RBD], Wondfo USA, Willowbrook, IL). A subset of samples was tested by the semi-quantitative Abbott AdviseDx SARS-CoV-2 IgG II assay (IgG against RBD). …”
Publicado 2021
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4853por Ayers, Emily C., Wilson, Jeffrey, Canderan, Glenda, Keshavarz, Behnam, Woodfolk, Judith, Portell, Craig A., Williams, Michael E.“…IgG to the SARS-CoV-2 spike receptor-binding domain (RBD) was quantified using the ImmunoCAP platform (Thermo Fisher); results were compared to data from 167 subjects in a healthy vaccine cohort at the University of Virginia. …”
Publicado 2021
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4854por Liang, Yan, Buchanan, Bailey C., Khanthaphixay, Bradley, Zhou, Avory, Quirk, Grace, Worobey, Michael, Yoon, Jeong-Yeol“…Competitive particle immunoassays were developed on a paper microfluidic chip using the receptor-binding domain (RBD) antigens on spike proteins. Using a smartphone, they were monitored by counting the captured fluorescent particles or evaluating the capillary flow velocities. …”
Publicado 2023
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4855por Cheng, Yan, Zheng, Dan, Zhang, Derong, Guo, Du, Wang, Yueteng, Liu, Wei, Liang, Li, Hu, Jianping, Luo, Ting“…The inefficient utilization of TMPRSS2 by Omicron is consistent with its primary entry into cells via the endosomal pathway. In addition, RBD-directed antibody C121 showed obvious resistance to Omicron, which may have originated from high fluctuation of approaching angles, high flexibility of I472-F490 loop, and reduced binding ability. …”
Publicado 2023
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4856por Esmagambetov, Ilias B., Ryabova, Ekaterina I., Derkaev, Artem A., Shcheblyakov, Dmitry V., Dolzhikova, Inna V., Favorskaya, Irina A., Grousova, Daria M., Dovgiy, Mikhail A., Prokofiev, Vladimir V., Gosudarev, Andrey I., Byrikhina, Daria V., Zorkov, Ilia D., Iliukhina, Anna A., Kovyrshina, Anna V., Shelkov, Artem Y., Naroditsky, Boris S., Logunov, Denis Y., Gintsburg, Alexander L.“…METHODS: In current work we developed a recombinant adeno-associated viral vector (rAAV), expressing a SARS-CoV-2 spike receptor-binding domain (RBD)-specific antibody P2C5 fused with a human IgG1 Fc fragment (P2C5-Fc) using methods of molecular biotechnology and bioprocessing. …”
Publicado 2023
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4857por Borghi, Martina, Gallinaro, Alessandra, Pirillo, Maria Franca, Canitano, Andrea, Michelini, Zuleika, De Angelis, Maria Laura, Cecchetti, Serena, Tinari, Antonella, Falce, Chiara, Mariotti, Sabrina, Capocefalo, Antonio, Chiantore, Maria Vincenza, Iacobino, Angelo, Di Virgilio, Antonio, van Gils, Marit J., Sanders, Rogier W., Lo Presti, Alessandra, Nisini, Roberto, Negri, Donatella, Cara, Andrea“…Anti-receptor binding domain (RBD) binding Abs, nAbs and T cell responses were detected up to six months from a single immunization with escalating doses of vaccines in all mice, but with different levels and kinetics. …”
Publicado 2023
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4858por Ingólfsson, Helgi I., Bhatia, Harsh, Aydin, Fikret, Oppelstrup, Tomas, López, Cesar A., Stanton, Liam G., Carpenter, Timothy S., Wong, Sergio, Di Natale, Francesco, Zhang, Xiaohua, Moon, Joseph Y., Stanley, Christopher B., Chavez, Joseph R., Nguyen, Kien, Dharuman, Gautham, Burns, Violetta, Shrestha, Rebika, Goswami, Debanjan, Gulten, Gulcin, Van, Que N., Ramanathan, Arvind, Van Essen, Brian, Hengartner, Nicolas W., Stephen, Andrew G., Turbyville, Thomas, Bremer, Peer-Timo, Gnanakaran, S., Glosli, James N., Lightstone, Felice C., Nissley, Dwight V., Streitz, Frederick H.“…To capture the driving forces that bring RAS and RAF (represented as two domains, RBD and CRD) together on the plasma membrane, simulations with the ability to calculate atomic detail while having long time and large length- scales are needed. …”
Publicado 2023
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4859por Rahim, N.A. Mat, Zahidi, J., Hashim, N.A. Amir, Amdan, N.A. Nor, Zamri, H.F., Ali, M.R. Mohd, Zain, R. Mohd, Ahmad, N.“…Selection of M protein perhaps a better option compared to current detection assays that use spike (S) protein, which could lead to false negative results, as this gene region particularly the ribosome-binding domain (RBD) rapidly undergoes mutations. The utilization of M protein potentially improves negative predictive value (NPV) of the diagnostic test. …”
Publicado 2023
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4860por Ramezani, Amitis, Sorouri, Rahim, Haji Maghsoudi, Saiedeh, Dahmardeh, Sarah, Doroud, Delaram, Sadat Larijani, Mona, Eybpoosh, Sana, Mostafavi, Ehsan, Olyaeemanesh, Alireza, Salehi-Vaziri, Mostafa, Bavand, Anahita, Zarghani, Ghazaleh, Moradi, Ladan, Ashrafian, Fatemeh, Bagheri Amiri, Fahimeh, Mashayekhi, Parisa, Tahmasebi, Zahra, Biglari, Alireza“…PastoCovac booster recipients achieved the highest rate of anti-Spike IgG titer rise with a fourfold rise in 50% of the group. Anti-RBD IgG and neutralizing antibody mean rise and fold rise were almost similar between the PastoCovac and PastoCovac Plus booster receivers. …”
Publicado 2023
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