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4881por Requena, David, Médico, Aldhair, Chacón, Ruy D., Ramírez, Manuel, Marín-Sánchez, Obert“…Additionally, we are presenting 13 HLA-I and 30 HLA-II novel candidate epitopes without experimental evidence, including 16 class-II candidates in highly exposed conserved areas of the NTD and RBD regions of the Spike protein. These novel candidates have even better coverage scores for South America than those with experimental evidence. …”
Publicado 2020
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4882por Martínez-Hernández, Fernando, Isaak-Delgado, Ana Belem, Alfonso-Toledo, Jorge Alberto, Muñoz-García, Claudia Irais, Villalobos, Guiehdani, Aréchiga-Ceballos, Nidia, Rendón-Franco, Emilio“…Interestingly, during this short pandemic period, a high haplotypic variation was observed in the RBD of the viral S protein, suggesting new risks for other hosts. …”
Publicado 2020
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4883por Hu, Fengyu, Chen, Fengjuan, Ou, Zhihua, Fan, Qinghong, Tan, Xinghua, Wang, Yaping, Pan, Yuejun, Ke, Bixia, Li, Linghua, Guan, Yujuan, Mo, Xiaoneng, Wang, Jian, Wang, Jinlin, Luo, Chun, Wen, Xueliang, Li, Min, Ren, Peidi, Ke, Changwen, Li, Junhua, Lei, Chunliang, Tang, Xiaoping, Li, Feng“…In contrast, viral rebound was associated with significantly lower levels of and slower generation of viral receptor-binding domain (RBD)-specific IgA and IgG antibodies. Our study demonstrated that the positive retest patients failed to create a robust protective humoral immune response, which might result in SARS-CoV-2 persistence in the gastrointestinal tract and possibly in active viral shedding. …”
Publicado 2020
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4884por Bošnjak, Berislav, Stein, Saskia Catherina, Willenzon, Stefanie, Cordes, Anne Katrin, Puppe, Wolfram, Bernhardt, Günter, Ravens, Inga, Ritter, Christiane, Schultze-Florey, Christian R., Gödecke, Nina, Martens, Jörg, Kleine-Weber, Hannah, Hoffmann, Markus, Cossmann, Anne, Yilmaz, Mustafa, Pink, Isabelle, Hoeper, Marius M., Behrens, Georg M. N., Pöhlmann, Stefan, Blasczyk, Rainer, Schulz, Thomas F., Förster, Reinhold“…Neutralizing antibodies targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) block severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry into cells via surface-expressed angiotensin-converting enzyme 2 (ACE2). …”
Publicado 2020
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4885por Demaret, Julie, Lefèvre, Guillaume, Vuotto, Fanny, Trauet, Jacques, Duhamel, Alain, Labreuche, Julien, Varlet, Pauline, Dendooven, Arnaud, Stabler, Sarah, Gachet, Benoit, Bauer, Jules, Prevost, Brigitte, Bocket, Laurence, Alidjinou, Enagnon Kazali, Lambert, Marc, Yelnik, Cécile, Meresse, Bertrand, Dubuquoy, Laurent, Launay, David, Dubucquoi, Sylvain, Montaigne, David, Woitrain, Eloise, Maggiotto, François, Bou Saleh, Mohamed, Top, Isabelle, Elsermans, Vincent, Jeanpierre, Emmanuelle, Dupont, Annabelle, Susen, Sophie, Brousseau, Thierry, Poissy, Julien, Faure, Karine, Labalette, Myriam“…The frequency of reactive T cells observed with M, S and MNS mix pools correlated with severity and with levels of anti‐S1 and anti‐RBD serum antibodies. CONCLUSION: IFNγ T‐CoV‐Spot assay is a reliable method to explore specific T cells in large cohorts of patients. …”
Publicado 2020
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4886por Li, Ling, Tan, Chianru, Zeng, Jia, Luo, Chen, Hu, Shi, Peng, Yanke, Li, Wenjuan, Xie, Zhixiong, Ling, Yueming, Zhang, Xuejun, Deng, E., Xu, Haixia, Wang, Jue, Xie, Yudi, Zhou, Yaling, Zhang, Wei, Guo, Yong, Liu, Zhong“…Meanwhile, anti-nucleocapsid protein (anti-N) IgM and IgG antibodies and anti-spike protein receptor-binding domain (anti-S-RBD) IgG antibody in all serum samples were tested using ELISA. …”
Publicado 2021
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4887por Zhang, Chao, Wang, Yifan, Zhu, Yuanfei, Liu, Caixuan, Gu, Chenjian, Xu, Shiqi, Wang, Yalei, Zhou, Yu, Wang, Yanxing, Han, Wenyu, Hong, Xiaoyu, Yang, Yong, Zhang, Xueyang, Wang, Tingfeng, Xu, Cong, Hong, Qin, Wang, Shutian, Zhao, Qiaoyu, Qiao, Weihua, Zang, Jinkai, Kong, Liangliang, Wang, Fangfang, Wang, Haikun, Qu, Di, Lavillette, Dimitri, Tang, Hong, Deng, Qiang, Xie, Youhua, Cong, Yao, Huang, Zhong“…Here, we report the identification and characterization of two groups of mouse neutralizing monoclonal antibodies (MAbs) targeting the receptor-binding domain (RBD) on the SARS-CoV-2 spike (S) protein. MAbs 2H2 and 3C1, representing the two antibody groups, respectively, bind distinct epitopes and are compatible in formulating a noncompeting antibody cocktail. …”
Publicado 2021
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4888por Atiq, F., Saes, J.L., Punt, M.C., van Galen, K.P.M., Schutgens, R.E.G., Meijer, K., Cnossen, M.H., Laros-Van Gorkom, B.A.P., Peters, M., Nieuwenhuizen, L., Kruip, M.J.H.A., de Meris, J., van der Bom, J.G., van der Meer, F.J.M., Fijnvandraat, K., Kruis, I.C., van Heerde, W.L., Eikenboom, H.C.J., Leebeek, Frank W.G., Schols, S.E.M.“…FINDINGS: We included 1092 patients (834 VWD; 196 RBD; 62 CPD), of whom 665 (60.9%) were women. Women were more often referred because of a bleeding diathesis than men (47.9% vs 36.6%, p = 0.002). …”
Publicado 2021
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4889por Bartsch, Yannic C., Fischinger, Stephanie, Siddiqui, Sameed M., Chen, Zhilin, Yu, Jingyou, Gebre, Makda, Atyeo, Caroline, Gorman, Matthew J., Zhu, Alex Lee, Kang, Jaewon, Burke, John S., Slein, Matthew, Gluck, Matthew J., Beger, Samuel, Hu, Yiyuan, Rhee, Justin, Petersen, Eric, Mormann, Benjamin, Aubin, Michael de St, Hasdianda, Mohammad A., Jambaulikar, Guruprasad, Boyer, Edward W., Sabeti, Pardis C., Barouch, Dan H., Julg, Boris D., Musk, Elon R., Menon, Anil S., Lauffenburger, Douglas A., Nilles, Eric J., Alter, Galit“…Notably, neutralization, Fc-function, and SARS-CoV-2 specific T cell responses were only observed in subjects that elicited RBD-specific antibody titers above a threshold. …”
Publicado 2021
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4890por Shastri, Jayanthi, Parikh, Swapneil, Agrawal, Sachee, Chatterjee, Nirjhar, Pathak, Manish, Chaudhary, Sakshi, Sharma, Chetan, Kanakan, Akshay, A, Vivekanand, Srinivasa Vasudevan, Janani, Maurya, Ranjeet, Fatihi, Saman, Thukral, Lipi, Agrawal, Anurag, Pinto, Lancelot, Pandey, Rajesh, Sunil, Sujatha“…Serial serology was performed for Anti-N IgG, Anti- S1 RBD IgG, and sVNT (surrogate virus neutralizing test). …”
Publicado 2021
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4891“…The differential antibody blocking of spike binding to AT2, PHH and HLC indicated that neutralizing activity of SARS-CoV-2 binding might involve additional mechanisms beyond RBD binding to ACE-2.…”
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4892por Scheid, Johannes F., Barnes, Christopher O., Eraslan, Basak, Hudak, Andrew, Keeffe, Jennifer R., Cosimi, Lisa A., Brown, Eric M., Muecksch, Frauke, Weisblum, Yiska, Zhang, Shuting, Delorey, Toni, Woolley, Ann E., Ghantous, Fadi, Park, Sung-Moo, Phillips, Devan, Tusi, Betsabeh, Huey-Tubman, Kathryn E., Cohen, Alexander A., Gnanapragasam, Priyanthi N.P., Rzasa, Kara, Hatziioanno, Theodora, Durney, Michael A., Gu, Xiebin, Tada, Takuya, Landau, Nathaniel R., West, Anthony P., Rozenblatt-Rosen, Orit, Seaman, Michael S., Baden, Lindsey R., Graham, Daniel B., Deguine, Jacques, Bieniasz, Paul D., Regev, Aviv, Hung, Deborah, Bjorkman, Pamela J., Xavier, Ramnik J.“…Cryo-electron microscopy structures of selected nAbs from these two clusters complexed with SARS-CoV-2 spike trimers show recognition of various receptor-binding domain (RBD) epitopes. One of these mAbs, BG10-19, locks the spike trimer in a closed conformation to potently neutralize SARS-CoV-2, the recently arising mutants B.1.1.7 and B.1.351, and SARS-CoV and cross-reacts with heterologous RBDs. …”
Publicado 2021
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4893por Gerhards, Catharina, Thiaucourt, Margot, Kittel, Maximilian, Becker, Celine, Ast, Volker, Hetjens, Michael, Neumaier, Michael, Haselmann, Verena“…For each participant, anti-nucleocapsid (N) and anti-spike subunit 1 receptor binding domain (RBD/S1) immunoglobulin (Ig) levels were regularly determined over a period of 8 months. …”
Publicado 2021
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4894por Ryu, Dong-Kyun, Song, Rina, Kim, Minsoo, Kim, Young-Il, Kim, Cheolmin, Kim, Jong-In, Kwon, Ki-Sung, Tijsma, Aloys SL., Nuijten, Patricia M., van Baalen, Carel A., Hermanus, Tandile, Kgagudi, Prudence, Moyo-Gwete, Thandeka, Moore, Penny L., Choi, Young Ki, Lee, Soo-Young“…CT-P59 showed reduced binding affinity against a RBD (receptor binding domain) triple mutant containing mutations defining B.1.351 (K417N/E484K/N501Y) also showed reduced potency against the SA variant in live virus and pseudovirus neutralization assay systems. …”
Publicado 2021
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4895por Jamiruddin, Mohd Raeed, Haq, Md Ahsanul, Tomizawa, Kazuhito, Kobatake, Eiry, Mie, Masayasu, Ahmed, Sohel, Khandker, Shahad Saif, Ali, Tamanna, Jahan, Nowshin, Oishee, Mumtarin Jannat, Khondoker, Mohib Ullah, Sil, Bijon Kumar, Haque, Mainul, Adnan, Nihad“…We have developed 12 different types of in-house ELISAs to observe the kinetics of IgG, IgM, and IgA against four SARS-CoV-2 proteins, namely nucleocapsid, RBD, S1, and whole spike (S1+S2). For the development of these assays, 30–104 pre-pandemic samples were taken as negative controls and 83 RT-qPCR positive samples as positive ones. …”
Publicado 2021
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4896por Jagtap, Suraj, K, Ratnasri, Valloly, Priyanka, Sharma, Rakhi, Maurya, Satyaghosh, Gaigore, Anushree, Ardhya, Chitra, Biligi, Dayananda S., Desiraju, Bapu Koundinya, Natchu, Uma Chandra Mouli, Saini, Deepak Kumar, Roy, Rahul“…METHODS: We compared four spike proteins (RBD, S1, S2 and a stabilized spike trimer (ST)) representing commonly used antigens for their reactivity to human IgG antibodies using indirect ELISA in serum from COVID-19 patients and pre-2020 samples. …”
Publicado 2021
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4897por Bullen, Georgia, Galson, Jacob D., Hall, Gareth, Villar, Pedro, Moreels, Lien, Ledsgaard, Line, Mattiuzzo, Giada, Bentley, Emma M., Masters, Edward W., Tang, David, Millett, Sophie, Tongue, Danielle, Brown, Richard, Diamantopoulos, Ioannis, Parthiban, Kothai, Tebbutt, Claire, Leah, Rachael, Chaitanya, Krishna, Ergueta-Carballo, Sandra, Pazeraitis, Deividas, Surade, Sachin B., Ashiru, Omodele, Crippa, Lucia, Cowan, Richard, Bowler, Matthew W., Campbell, Jamie I., Lee, Wing-Yiu Jason, Carr, Mark D., Matthews, David, Pfeffer, Paul, Hufton, Simon E., Sawmynaden, Kovilen, Osbourn, Jane, McCafferty, John, Karatt-Vellatt, Aneesh“…Finally, by combining sequences of the RBD binding and neutralizing antibodies with the B cell receptor repertoire sequencing, we also describe a highly convergent early antibody response. …”
Publicado 2021
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4898por Perez-Saez, Javier, Zaballa, María-Eugenia, Yerly, Sabine, Andrey, Diego O., Meyer, Benjamin, Eckerle, Isabella, Balavoine, Jean-François, Chappuis, François, Pittet, Didier, Trono, Didier, Kherad, Omar, Vuilleumier, Nicolas, Kaiser, Laurent, Guessous, Idris, Stringhini, Silvia, Azman, Andrew S.“…METHODS: We followed a cohort of mostly mild/asymptomatic SARS-CoV-2-infected individuals (n = 354) at least 8 months after their presumed infection date and tested their serum for anti-SARS-CoV-2 antibodies with three commercially available assays: Roche-N, Roche-RBD and EuroImmun-S1. We developed a latent class statistical model to infer the specificity and time-varying sensitivity of each assay and show through simulations how inappropriately accounting for test performance can lead to biased serosurvey estimates. …”
Publicado 2021
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4899por Pulakuntla, Swetha, Lokhande, Kiran Bharat, Padmavathi, Pannuru, Pal, Meena, Swamy, Kakumani Venkateswara, Sadasivam, Jayashree, Singh, Shri Abhiav, Aramgam, Sree Latha, Reddy, Vaddi Damodara“…The spike receptor binding protein (RBD) used as target region for our studies with FDA-approved drugs for repurposing, and identified few anti-SARS-CoV2 potential drugs (Silmitasertib, AC-55541, Merimepodib, XL413, AZ3451) based on their docking score and binding mode calculations expected to strongly bind to motifs of ACE2 receptor and may show impart relief in COVID-19 patients.…”
Publicado 2021
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4900por Aljindan, Reem Y., Al-Subaie, Abeer M., Al-Ohali, Ahoud I., Kumar D, Thirumal, Doss C, George Priya, Kamaraj, Balu“…The receptor-binding domain (RBD) of the Spike-protein (S-protein) interacts with the human cells through the host angiotensin-converting enzyme 2 (ACE2) receptor. …”
Publicado 2021
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