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4921por Kruse, Robert L., Huang, Yuting, Lee, Alyssa, Zhu, Xianming, Shrestha, Ruchee, Laeyendecker, Oliver, Littlefield, Kirsten, Pekosz, Andy, Bloch, Evan M., Tobian, Aaron A. R., Wang, Zack Z.“…The sensitivities of the hemagglutination test, Euroimmun IgG enzyme-linked immunosorbent assay (ELISA), and receptor binding domain (RBD)-based CoronaChek lateral flow assay were 87.0%, 86.5%, and 84.5%, respectively, using samples obtained from recovered COVID-19 individuals. …”
Publicado 2021
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4922por Li, Fang Fang, Liu, Aaron, Gibbs, Ebrima, Tanunliong, Guadalein, Marquez, Ana Citlali, Gantt, Soren, Frykman, Hans, Krajden, Mel, Morshed, Muhammad, Prystajecky, Natalie A., Cashman, Neil, Sekirov, Inna, Jassem, Agatha N.“…RESULTS: We observed SARS-CoV-2 target sensitivities of 100% and specificities >94% for all antigens (RBD, Nucleocapsid, Spike) in V-PLEX Panel 2. When targets were combined, we found a SARS-CoV-2 sensitivity of 100% and specificity of 98.8% with no difference in performance compared to clinical assays, and Cohen's kappa ranging from 0.798 to 0.945 compared to surface plasmon resonance imaging (SPRi). …”
Publicado 2022
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4923por Gurevich, Michael, Zilkha-Falb, Rina, Sonis, Polina, Magalashvili, David, Menascu, Shay, Flechter, Shlomo, Dolev, Mark, Mandel, Mathilda, Achiron, Anat“…SARS-CoV-2 anti-spike IgG was determined by ELISA, MBC by SARS-CoV-2-specific receptor binding domain (RBD) ELISpot, and interferon gamma (IFN-γ)-, interleukin 2 (IL2)-, and IFN-γ+IL2-secreting MTC by IFN-γ and IL2 SARS-CoV-2 FluoroSpot. …”
Publicado 2022
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4924por Pan, Ting, Chen, Ran, He, Xin, Yuan, Yaochang, Deng, Xiaohui, Li, Rong, Yan, Haiping, Yan, Shumei, Liu, Jun, Zhang, Yiwen, Zhang, Xiantao, Yu, Fei, Zhou, Mo, Ke, Changwen, Ma, Xiancai, Zhang, Hui“…Mechanistically, the receptor-binding domain (RBD) of SARS-CoV-2 (B.1.351) spike protein turned to a high binding affinity to mouse angiotensin-converting enzyme 2 (mACE2), allowing the mice highly susceptible to SARS-CoV-2 (B.1.351) infection. …”
Publicado 2021
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4925por Tang, Li, Cherry, Sean, Tuomanen, Elaine I, Kirkpatrick Roubidoux, Ericka, Lin, Chun Yang, Allison, Kim J, Gowen, Ashleigh, Freiden, Pamela, Allen, E Kaitlynn, Su, Yin, Gaur, Aditya H, Estepp, Jeremie H, McGargill, Maureen A, Krammer, Florian, Thomas, Paul G, Schultz-Cherry, Stacey, Wolf, Joshua“…Jude Children’s Research Hospital, we identified participants with plasma samples collected after SARS-CoV-2 infection, after mRNA vaccination, and after vaccination following infection, and quantitated immunoglobulin G (IgG) levels by enzyme-linked immunosorbent assay to the spike receptor binding domain (RBD) from 5 important SARS-CoV-2 variants (Wuhan Hu-1, B.1.1.7, B.1.351, P.1, and B.1.617.2). …”
Publicado 2021
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4926por Ferrara, P., Ponticelli, D., Agüero, F., Caci, G., Vitale, A., Borrelli, M., Schiavone, B., Antonazzo, I.C., Mantovani, L.G., Tomaselli, V., Polosa, R.“…METHODS: HCWs who were administered two doses of the BNT162b2 mRNA vaccine, 21 days apart, between December 2020 and January 2021, were invited to undergo multiple serology tests to identify SARS-CoV-2 S-RBD-specific immunoglobulin G (IgG) antibodies. …”
Publicado 2022
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4927por Yan, Feihu, Li, Entao, Wang, Tiecheng, Li, Yuanguo, Liu, Jun, Wang, Weiqi, Qin, Tian, Su, Rina, Pei, Hongyan, Wang, Shen, Feng, Na, Zhao, Yongkun, Yang, Songtao, Xia, Xianzhu, Gao, Yuwei“…Q489H substitution in the receptor-binding domain (RBD) of BMA8 and C57MA14 variants results in the receptors of SARS-CoV-2 switching from human angiotensin-converting enzyme 2 (hACE2) to murine angiotensin-converting enzyme 2 (mACE2). …”
Publicado 2022
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4928por Jawalagatti, Vijayakumar, Kirthika, Perumalraja, Hewawaduge, Chamith, Park, Ji-Young, Yang, Myeon-Sik, Oh, Byungkwan, So, Mi Young, Kim, Bumseok, Lee, John Hwa“…The multicistronic vaccine design enabled by the viral self-cleaving peptides targets receptor binding domain (RBD), heptad repeat domain (HR), membrane glycoprotein (M) and epitopes of nsp13 of parental SARS-CoV-2. …”
Publicado 2022
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4929por Kok, Kin-Hang, Wong, Shuk-Ching, Chan, Wan-Mui, Wen, Lei, Chu, Allen Wing-Ho, Ip, Jonathan Daniel, Lee, Lam-Kwong, Wong, Ivan Tak-Fai, Lo, Hazel Wing-Hei, Cheng, Vincent Chi-Chung, Ho, Alex Yat-Man, Lam, Bosco Hoi-Shiu, Tse, Herman, Lung, David, Ng, Ken Ng Ho-Leung, Au, Albert Ka-Wing, Siu, Gilman Kit-Hang, Yuen, Kwok-Yung“…While no hamster-to-human transmission of B.1.258-like strain was found in this outbreak, molecular docking showed that its spike receptor-binding domain (RBD) has a similar binding energy to human ACE2 compared to that of Delta variant AY.127. …”
Publicado 2022
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4930por Benning, Louise, Klein, Katrin, Morath, Christian, Bartenschlager, Marie, Kim, Heeyoung, Buylaert, Mirabel, Reineke, Marvin, Töllner, Maximilian, Nusshag, Christian, Kälble, Florian, Reichel, Paula, Schnitzler, Paul, Zeier, Martin, Süsal, Caner, Bartenschlager, Ralf, Schaier, Matthias, Speer, Claudius“…Triple seropositivity for anti-S1 IgG, surrogate neutralizing, and anti-RBD antibodies increased from 31/84 (37%) dialysis patients after second to 80/84 (95%) after third vaccination. …”
Publicado 2022
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4931por Iijima, Takahiro, Ando, Shinnosuke, Kanamori, Dai, Kuroda, Kazumichi, Nomura, Tsutomu, Tisi, Laurence, Kilgore, Paul E., Percy, Neil, Kohase, Hikaru, Hayakawa, Satoshi, Seki, Mitsuko, Hoshino, Tomonori“…The new coronavirus infection (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be fatal, and several variants of SARS-CoV-2 with mutations of the receptor-binding domain (RBD) have increased avidity for human cell receptors. …”
Publicado 2022
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4932por Wong, Ting Y., Horspool, Alexander M., Russ, Brynnan P., Ye, Chengjin, Lee, Katherine S., Winters, Michael T., Bevere, Justin R., Miller, Olivia A., Rader, Nathaniel A., Cooper, Melissa, Kieffer, Theodore, Sourimant, Julien, Greninger, Alexander L., Plemper, Richard K., Denvir, James, Cyphert, Holly A., Barbier, Mariette, Torrelles, Jordi B., Martinez, Ivan, Martinez-Sobrido, Luis, Damron, F. Heath“…HCP treatment groups had elevated receptor binding domain (RBD) and nucleocapsid IgG titers in the serum; however, Beta VoC viral RNA burden in the lung and brain was not decreased due to HCP treatment. …”
Publicado 2022
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4933por Peng, Lei, Hu, Yingxia, Mankowski, Madeleine C., Ren, Ping, Chen, Rita E., Wei, Jin, Zhao, Min, Li, Tongqing, Tripler, Therese, Ye, Lupeng, Chow, Ryan D., Fang, Zhenhao, Wu, Chunxiang, Dong, Matthew B., Cook, Matthew, Wang, Guilin, Clark, Paul, Nelson, Bryce, Klein, Daryl, Sutton, Richard, Diamond, Michael S., Wilen, Craig B., Xiong, Yong, Chen, Sidi“…By high-throughput single cell sequencing of B cells from spike receptor binding domain (RBD) immunized animals, we identify two highly potent SARS-CoV-2 neutralizing mAb clones that have single-digit nanomolar affinity and low-picomolar avidity, and generate a bispecific antibody. …”
Publicado 2022
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4934“…In contrast, both the S1-CTD/RBD, which binds ACE2, and the S2 subunit (stalk), failed to mediate the same effect. …”
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4935por Glück, Vivian, Tydykov, Leonid, Mader, Anna-Lena, Warda, Anne-Sophie, Bertok, Manuela, Weidlich, Tanja, Gottwald, Christine, Köstler, Josef, Salzberger, Bernd, Wagner, Ralf, Koller, Michael, Gessner, André, Schmidt, Barbara, Glück, Thomas, Peterhoff, David“…RESULTS: Anti-receptor-binding domain (RBD) antibody titers decline over time in dually vaccinated COVID-19 naïves whereas titers in single dose vaccinated COVID-19 convalescents are higher and more durable. …”
Publicado 2022
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4936por Kornek, Barbara, Leutmezer, Fritz, Rommer, Paulus S., Koblischke, Maximilian, Schneider, Lisa, Haslacher, Helmuth, Thalhammer, Renate, Zimprich, Fritz, Zulehner, Gudrun, Bsteh, Gabriel, Dal‐Bianco, Assunta, Rinner, Walter, Zebenholzer, Karin, Wimmer, Isabella, Steinmaurer, Anja, Graninger, Marianne, Mayer, Margareta, Roedl, Kilian, Berger, Thomas, Winkler, Stefan, Aberle, Judith H., Tobudic, Selma“…For quantification of antibodies, the Elecsys anti‐SARS‐CoV‐2 viral spike (S) immunoassay against the receptor‐binding domain (RBD) was used. IFN‐gamma enzyme‐linked immunosorbent spot assays were performed to assess T cell responses against the SARS‐CoV‐2 Wuhan strain and the Delta variant. …”
Publicado 2022
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4937por Chekol Abebe, Endeshaw, Tiruneh G/Medhin, Markeshaw, Behaile T/Mariam, Awgichew, Asmamaw Dejenie, Tadesse, Mengie Ayele, Teklie, Tadele Admasu, Fitalew, Tilahun Muche, Zelalem, Asmare Adela, Getachew“…Half of the mutations in the S protein are in the RBD, which is a major target of antibodies, showing that Omicron mutations may affect antibody binding affinity to the S protein. …”
Publicado 2022
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4938“…BACKGROUND: Coronavirus disease 2019 (COVID-19) is caused by severe acute –respiratory syndrome coronavirus 2 (SARS- CoV-2) through interaction of the spike protein (SP) with the receptor-binding domain (RBD) and its receptor, angiotensin converting enzyme 2(ACE2). …”
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4939por Saker, Kahina, Pozzetto, Bruno, Escuret, Vanessa, Pitiot, Virginie, Massardier-Pilonchéry, Amélie, Mokdad, Bouchra, Langlois-Jacques, Carole, Rabilloud, Muriel, Alfaiate, Dulce, Guibert, Nicolas, Fassier, Jean-Baptiste, Bal, Antonin, Trouillet-Assant, Sophie, Trabaud, Mary-Anne“…Despite the good correlation between the anti-spike antibody titer and the titer of NAb by PRNT(50), our results highlight the difficulty to distinguish true NAb among the anti-RBD antibodies with commercial user-friendly immunoassays.…”
Publicado 2022
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4940por Hany, Mohamed, Torensma, Bart, Abouelnasr, Anwar Ashraf, Zidan, Ahmed, Ibrahim, Mohamed, Agayby, Ann Samy Shafiq, Hesham, Mohamed, Elsheredy, Amel, Abu-Sheasha, Ghada Ahmed“…Quantitative antibodies against SARS‑CoV‑2 RBD with a strong neutralizing capacity were quantified from sera after at least 2 weeks post-vaccination. …”
Publicado 2022
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