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5001por Puhl, Ana C., Fritch, Ethan James, Lane, Thomas R., Tse, Longping V., Yount, Boyd L., Sacramento, Carol Queiroz, Tavella, Tatyana Almeida, Costa, Fabio Trindade Maranhão, Weston, Stuart, Logue, James, Frieman, Matthew, Premkumar, Lakshmanane, Pearce, Kenneth H., Hurst, Brett L., Andrade, Carolina Horta, Levi, James A., Johnson, Nicole J., Kisthardt, Samantha C., Scholle, Frank, Souza, Thiago Moreno L., Moorman, Nathaniel John, Baric, Ralph S., Madrid, Peter, Ekins, Sean“…We have also tested them in a pseudovirus assay and used microscale thermophoresis to test the binding of these molecules to the spike protein. They bind to spike RBD protein with K(d) values of 339 nM and 647 nM, respectively. …”
Publicado 2020
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5002por Young, Mary K., Kornmeier, Christine, Carpenter, Rebecca M., Natale, Nick R., Sasson, Jennifer M., Solga, Michael D., Mathers, Amy J., Poulter, Melinda D., Qiang, Xiao, Petri, William A.“…Antibody testing is essential to identify persons exposed to the virus and potentially in predicting disease immunity. 183 COVID-19 patients (68 of whom required mechanical ventilation) and 41 controls were tested for plasma IgG, IgA and IgM against the SARS-CoV-2 S1, S2, receptor binding domain (RBD) and N proteins using the MILLIPLEX(®) SARS-CoV-2 Antigen Panel. …”
Publicado 2020
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5003por Kalish, Heather, Klumpp-Thomas, Carleen, Hunsberger, Sally, Baus, Holly Ann, Fay, Michael P, Siripong, Nalyn, Wang, Jing, Hicks, Jennifer, Mehalko, Jennifer, Travers, Jameson, Drew, Matthew, Pauly, Kyle, Spathies, Jacquelyn, Ngo, Tran, Adusei, Kenneth M., Karkanitsa, Maria, Croker, Jennifer A, Li, Yan, Graubard, Barry I., Czajkowski, Lindsay, Belliveau, Olivia, Chairez, Cheryl, Snead, Kelly, Frank, Peter, Shunmugavel, Anandakumar, Han, Alison, Giurgea, Luca T., Rosas, Luz Angela, Bean, Rachel, Athota, Rani, Cervantes-Medina, Adriana, Gouzoulis, Monica, Heffelfinger, Brittany, Valenti, Shannon, Caldararo, Rocco, Kolberg, Michelle M., Kelly, Andrew, Simon, Reid, Shafiq, Saifullah, Wall, Vanessa, Reed, Susan, Ford, Eric W, Lokwani, Ravi, Denson, John-Paul, Messing, Simon, Michael, Sam G., Gillette, William, Kimberly, Robert P., Reis, Steven E., Hall, Matthew D., Esposito, Dominic, Memoli, Matthew J., Sadtler, Kaitlyn“…Samples were analyzed via ELISA for anti-Spike and anti-RBD antibodies. Estimation of seroprevalence was performed by using a weighted analysis to reflect the US population. …”
Publicado 2021
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5004por Mendoza, Rachelle, Silver, Michael, Zuretti, Alejandro R, Christian, Manan, Das, Ballabh, Norin, Allen J, Borgen, Patrick, Libien, Jenny, Bluth, Martin H“…Aliquots from the same units were subjected to enzyme-linked immunosorbent assay (ELISA) that detects IgG antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 S1 protein. The relationship between ELISA titers and CLIA S/Co values was analyzed using linear regression and receiver operating characteristics (ROC) curve. …”
Publicado 2021
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5005por Song, Deyong, Wang, Wenbo, Dong, Chuangchuang, Ning, Zhenfei, Liu, Xiu, Liu, Chuan, Du, Guangying, Sha, Chunjie, Wang, Kailin, Lu, Jun, Sun, Baiping, Zhao, Yanyan, Wang, Qiaoping, Xu, Hongguang, Li, Ying, Shen, Zhenduo, Jiao, Jie, Wang, Ruiying, Tian, Jingwei, Liu, Wanhui, Wang, Lan, Deng, Yong-Qiang, Dou, Changlin“…Structural analysis by cryo-EM revealed that CA521(FALA) recognizes an epitope overlapping with angiotensin converting enzyme 2 (ACE2)-binding sites in SARS-CoV-2 RBD in the Spike protein. CA521(FALA) blocks the interaction by binding all three RBDs of one SARS-CoV-2 spike trimer simultaneously. …”
Publicado 2021
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5006por Soni, Shobha, Kumar, Ashwani, Sehrawat, Nirmala, Kumar, Arvind, Kumar, Naresh, Lata, Charu, Mann, Anita“…Experiment was designed as RBD in three replications to evaluate 15 wheat genotypes with moderate saline irrigation (EC(iw) – 6 dS m(−1)) and extreme saline irrigation (EC(iw) – 10 dS m(−1)) along with one set of control (Best available water). …”
Publicado 2021
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5007por Vusirikala, Amoolya, Whitaker, Heather, Jones, Samuel, Tessier, Elise, Borrow, Ray, Linley, Ezra, Hoschler, Katja, Baawuah, Frances, Ahmad, Shazaad, Andrews, Nick, Ramsay, Mary, Ladhani, Shamez N, Brown, Kevin E, Amirthalingam, Gayatri“…Blood samples for SARS-CoV-2 antibody testing were obtained using a self-sampling kit and analysed using the Abbott SARS-CoV-2 N antibody and/or an in-house receptor binding domain (RBD) assay. FINDINGS: SARS-CoV-2 seroprevalence in 2,905 university students was 17.8% (95%CI, 16.5–19.3), ranging between 7.6%-29.7% across the five universities. …”
Publicado 2021
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5008por Verkerke, Hans, Horwath, Michael, Saeedi, Bejan, Boyer, Darra, Allen, Jerry W., Owens, Joshua, Arthur, Connie M., Nakahara, Hirotomo, Rha, Jennifer, Patel, Kashyap, Wu, Shang-Chuen, Paul, Anu, Yasin, Nini, Wang, Jianmei, Shin, Sooncheon, Brown, DeAndre, Normile, Katherine, Cole, Lisa, Meyers, Mark, Lin, Heather, Woods, Emily, Isaac, Jennifer, Broder, Kari, Wade, Jenna, Kauffman, Robert C., Patel, Ravi, Josephson, Cassandra D., Reynolds, Stacian, Sherman, Melanie, Wrammert, Jens, Alter, David, Guarner, Jeannette, Roback, John D., Neish, Andrew, Stowell, Sean R.“…Serological tests for immunoglobulin G (IgG), IgA, and IgM antibodies against the receptor binding domain (RBD) of SARS-CoV-2 were evaluated using samples from real-time reverse transcription-quantitative PCR (qRT-PCR)-confirmed inpatient coronavirus disease 2019 (COVID-19) cases. …”
Publicado 2021
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5009por Goel, Rishi R., Apostolidis, Sokratis A., Painter, Mark M., Mathew, Divij, Pattekar, Ajinkya, Kuthuru, Oliva, Gouma, Sigrid, Hicks, Philip, Meng, Wenzhao, Rosenfeld, Aaron M., Dysinger, Sarah, Lundgreen, Kendall A., Kuri-Cervantes, Leticia, Adamski, Sharon, Hicks, Amanda, Korte, Scott, Oldridge, Derek A., Baxter, Amy E., Giles, Josephine R., Weirick, Madison E., McAllister, Christopher M., Dougherty, Jeanette, Long, Sherea, D’Andrea, Kurt, Hamilton, Jacob T., Betts, Michael R., Luning Prak, Eline T., Bates, Paul, Hensley, Scott E., Greenplate, Allison R., Wherry, E. John“…Memory B cells specific for full-length spike protein and the spike receptor binding domain (RBD) were also efficiently primed by mRNA vaccination and detectable in all SARS-CoV-2 naive subjects after the second vaccine dose, though the memory B cell response declined slightly with age. …”
Publicado 2021
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5010por Castro Dopico, X., Muschiol, S., Christian, M., Hanke, L., Sheward, D. J., Grinberg, N. F., Rorbach, J., Bogdanovic, G., Mcinerney, G. M., Allander, T., Wallace, C., Murrell, B., Albert, J., Karlsson Hedestam, G. B.“…Sera from all participants and a cohort of historical (negative) controls (n = 595) were screened for IgG responses against stabilized trimers of the SARS‐CoV‐2 spike (S) glycoprotein and the smaller receptor‐binding domain (RBD). As a complement to standard analytical approaches, a probabilistic (cut‐off independent) Bayesian framework that assigns likelihood of past infection was used to analyse data over time. …”
Publicado 2021
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5011por Triest, David, Geebelen, Laurence, De Pauw, Robby, De Craeye, Stéphane, Vodolazkaia, Alexandra, Verbrugghe, Mathieu, Magerman, Koen, Robben, Lara-Lauren, Pannus, Pieter, Neven, Kristof, Ramaekers, Dirk, Van Gucht, Steven, Dierick, Katelijne, Van Loon, Nele, Goossens, Maria E., Desombere, Isabelle“…The OrientGene, which measures anti-RBD antibodies, can be valuable after vaccination as well.…”
Publicado 2021
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5012por Zhao, Juan, Wang, Ling, Schank, Madison, Dang, Xindi, Lu, Zeyuan, Cao, Dechao, Khanal, Sushant, Nguyen, Lam N., Nguyen, Lam N.T., Zhang, Jinyu, Zhang, Yi, Adkins, James L., Baird, Evan M., Wu, Xiao Y., Ning, Shunbin, Gazzar, Mohamed El, Moorman, Jonathan P., Yao, Zhi Q.“…Notably, SARS-CoV-2 specific T cell responses positively correlated with B cell IgG and neutralizing antibody responses to the receptor-binding domain (RBD) of the S protein. Our results demonstrate that defined levels of SARS-CoV-2 specific T cell responses are generated in some, but not all, COVID-19-recovered subjects, fostering hope for the protection of a proportion of COVID-19-exposed individuals against reinfection. …”
Publicado 2021
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5013por Gyebi, Gideon A., Ogunyemi, Oludare M., Ibrahim, Ibrahim M., Ogunro, Olalekan B., Adegunloye, Adegbenro P., Afolabi, Saheed O.“…Two pentacyclic terpenoids (24-methylene cycloartenol and isoiguesteri) interacted with the hACE2 binding hotspots for the SARS-CoV-2 spike protein, while the abietane diterpenes were found accommodated within the S1-specificity pocket, interacting strongly with the active site residues TMPRSS2. 3-benzoylhosloppone and cucurbitacin interacted with the RBD and S2 subunit of SARS-CoV-2 spike protein respectively. …”
Publicado 2021
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5014por Kannenberg, Judith, Schnurra, Carolin, Reiners, Nina, Henschler, Reinhard, Buhmann, Raymund, Kaiser, Thorsten, Biemann, Ronald, Hönemann, Mario, Ackermann, Grit, Trawinski, Henning, Jassoy, Christian“…The nucleoprotein-based Roche and the glycoprotein-based Abbott receptor binding domain (RBD) and Siemens tests were more sensitive than the N-based Abbott and the Euroimmun antibody tests (p = 0.0001 to p = 0.039). …”
Publicado 2021
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5015por Liu, Jinbiao, Bodnar, Brittany H., Meng, Fengzhen, Khan, Adil I., Wang, Xu, Saribas, Sami, Wang, Tao, Lohani, Saroj Chandra, Wang, Peng, Wei, Zhengyu, Luo, Jinjun, Zhou, Lina, Wu, Jianguo, Luo, Guangxiang, Li, Qingsheng, Hu, Wenhui, Ho, Wenzhe“…Mechanistic studies revealed that EGCG blocked infection at the entry step through interfering with the engagement of the receptor binding domain (RBD) of the viral spikes to angiotensin-converting enzyme 2 (ACE2) receptor of the host cells. …”
Publicado 2021
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5016por Ghareeb, Doaa A., Saleh, Samar R., Seadawy, Mohamed G., Nofal, Mohammed S., Abdulmalek, Shaymaa A., Hassan, Salma F., Khedr, Shaimaa M., AbdElwahab, Miral G., Sobhy, Ahmed A., Abdel-Hamid, Ali saber Ali, Yassin, Abdelrahman Mohamed, Elmoneam, Alshimaa A. Abd, Masoud, Aliaa A., Kaddah, Mohamed M. Y., El-Zahaby, Sally A., Al-mahallawi, Abdulaziz Mohsen, El-Gharbawy, Alaa M., Zaki, Ahmed, Seif, Inas k., Kenawy, Marwa Y., Amin, Magdy, Amer, Khaled, El Demellawy, Maha Adel“…Then in vitro studies on the two compounds were conducted including VeroE6 toxicity, anti-COVID-19 activity, determination of inhibitory activity towards papain-like proteinase (PL pro) and spike protein- and receptor- binding domain (RBD) as well as assessment of drug toxicity on RBCs. …”
Publicado 2021
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5017por Ruether, Darius F., Schaub, Golda M., Duengelhoef, Paul M., Haag, Friedrich, Brehm, Thomas T., Fathi, Anahita, Wehmeyer, Malte, Jahnke-Triankowski, Jacqueline, Mayer, Leonie, Hoffmann, Armin, Fischer, Lutz, Addo, Marylyn M., Lütgehetmann, Marc, Lohse, Ansgar W., Schulze zur Wiesch, Julian, Sterneck, Martina“…METHODS: In this prospective cohort study, anti-SARS-CoV-2 spike-protein titers were determined using the DiaSorin LIAISON (anti-S trimer) and Roche Elecsys (anti-S RBD) immunoassays in 194 patients (141 LT, 53 cirrhosis Child-Pugh A-C) and 56 healthy controls before and 10 to 84 days after second vaccination. …”
Publicado 2022
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5018por Daoudi, Kais, Ramachandran, Krithikadevi, Alawadhi, Hussain, Boukherroub, Rabah, Dogheche, Elhadj, Khakani, My Ali El, Gaidi, Mounir“…The current study encompasses the fabrication of a SERS sensor to study the SARS-CoV-2 S1 (RBD) spike protein of the SARS-CoV-2 virus family. …”
Publicado 2021
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5019por Fisher, Morly, Levy, Haim, Fatelevich, Ella, Afrimov, Yafa, Ben-Shmuel, Amir, Rosenfeld, Ronit, Noy-Porat, Tal, Glinert, Itai, Sittner, Assa, Biber, Asaf, Belkin, Ana, Bar-David, Elad, Puni, Reut, Levy, Itzchak, Mazor, Ohad, Weiss, Shay, Mechaly, Adva“…Sera from 55 symptomatic COVID-19 patients and 146 healthy subjects (early-pandemic, reverse transcription-quantitative PCR [qRT-PCR]-negative), collected in Israel between March and April 2020, were screened for SARS-CoV-2-specific IgG, IgM, and IgA antibodies, using a 6-plex antigen microarray presenting the whole inactivated virus and five viral antigens: a stabilized version of the spike ectodomain (S2P), spike subunit 1 (S1), receptor-binding-domain (RBD), N-terminal-domain (NTD), and nucleocapsid (NC). …”
Publicado 2021
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5020por Zhang, Li, Cui, Zhimin, Li, Qianqian, Wang, Bo, Yu, Yuanling, Wu, Jiajing, Nie, Jianhui, Ding, Ruxia, Wang, Haixin, Zhang, Yue, Liu, Shuo, Chen, Zhihai, He, Yaqing, Su, Xiaodong, Xu, Wenbo, Huang, Weijin, Wang, Youchun“…The activities of furin, TMPRSS2, and cathepsin L are increased against most of the variants. RBD amino acid mutations comprising K417T/N, L452R, Y453F, S477N, E484K, and N501Y cause significant immune escape from 11 of 13 monoclonal antibodies. …”
Publicado 2021
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