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5181“…To this aim, currently we are testing Ceph to run the Virtual Machines, both using RBD and Ceph-FS protocols, and to implement the object storage. …”
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5182por Whitaker, Jennifer, Rebolledo, Paulina, Rouphael, Nadine, Abate, Getahun, Babu, Tara M, Wald, Anna, Sahly, Hana El, Garbes, Pedro, Jooss, Karin, Allen, Andrew, McQuarrie, Lisa, Sitaula, Ranjan, Roberts, Paul C, Makhene, Mamodikoe, Posavad, Christine M, Juliana McElrath, M, De Rosa, Stephen, Coler, Rhea, Montefiori, David, Eaton, Amanda, Koelle, David M, Hoft, Daniel F“…Humoral (ELISA IgG against SARS-CoV-2 RBD and neutralizing antibody against D614G and Omicron BA.4/5) are being assessed at multiple time points over 1 year after study vaccination. …”
Publicado 2023
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5183por Khanna, Nina, Weisser, Maja, Hedstueck, Anemone, Sutter, Sarah Tschudin, Roesch, Sandra, Battegay, Manuel, Infanti, Laura, Holbro, Andreas, Busch, Michael Paul, Simmons, Graham, Felgner, Philip, Stone, Mars, von Goetz, Melissa, de Assis, Rafael, Khan, Saahir, Tsai, Jason, Robinson, Peter, Seftel, David, Bagri, Anil, Corash, Laurence, Buser, Andreas S.“…Only 2 of 15 CP cohort patients had detectable IgG antibody to SARS CoV-2 S1 antigen at study entry. 3 of 15 PI CP donors had negligible SARS CoV-2 IgG antibodies to all antigens, and demonstrated poor neutralization efficacy. 12/15 CP had effective RVPN titers (> 1:80), RVPN titers were correlated with ACE-2 neutralization antibody titers (r(2) = 0.83), and had significant activity specific for S and RBD antigens by microarray profiling (Figure 1). …”
Publicado 2020
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5184por Seror, R., Camus, M., Salmon, J.H., Roux, C., Dernis, E., Basch, A., Germain, V., Leské, C., Brousseau, S., Truchetet, M.E., Ramon, A., Gottenberg, J.E., Felten, R., Coury, F., Colombey, A., Prati, C., Mariette, X., Avouac, J.“…Les taux d’anticorps IgG ou totaux anti-protéine spike (S1, S1/S2 ou RBD) ont été détectés à l’aide de kits commerciaux utilisant majoritairement la technique d’immunoanalyse par chimiluminescence, ou dans 4 cas par ELISA ou enzyme linked fluorescence assay (ELFA). …”
Publicado 2021
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5185por Chelysheva, Ekaterina Yu., Petrova, Anna, Shukhov, Oleg A., Gurianova, Margarita, Bykova, Anastasiya, Nemchenko, Irina, Tsyba, Nikolay, Tikhomirov, Dmitry S., Turkina, Anna G.“…A semi-quantitative test detecting the SARS-CoV-2 S1 subunit (RBD) IgG antibodies by enzyme-linked immunoassay (ELISA) kit was used in the clinic. …”
Publicado 2021
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5186por Rotterdam, Jil, Thiaucourt, Margot, Schwaab, Juliana, Reiter, Andreas, Kreil, Sebastian, Steiner, Laurenz, Popp, Henning D., Hofmann, Wolf-Karsten, Bonatz, Karin, Gerhards, Catharina, Neumaier, Michael, Jawhar, Mohamad, Saussele, Susanne“…A FDA/CE approved electrochemiluminescent assay (ECLIA) (Elecsys®, Roche, Mannheim, Germany) was used to quantify antibodies, pan Ig (including IgG) against the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. The assay has a measurement range of 0.4 to 250 U/mL, with a concentration ≥0.8 U/ml considered as positive. …”
Publicado 2021
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5187por Cicin-Sain, Caroline, Huang, Alice, Pasin, Chloe, Epp, Selina, Mueller, Nicolas J, Nilsson, Jakob, Vilinovszki, Oliver, Nair, Gayathri, Wolfensberger, Nathan, Hockl, Philipp, Schanz, Urs, Trkola, Alexandra, Kouyos, Roger, Hasse, Barbara, Zinkernagel, Annelies, Manz, Markus G, Abela, Irene A, Mueller, Antonia MS“…Serial AB titers (prior to (T0); 1m after 1 (st) dose (T1); 1m (T2), 3m (T3), 6m (T4) post 2 (nd) dose) were measured with an in-house developed multiplex Antibody CORonavirus Assay (ABCORA) that measures SARS-CoV-2 IgG, IgA, and IgM reactivities against RBD (receptor binding domain), S1 (subunit 1 of the spike protein), S2 (subunit 2 of the spike protein) and N (nucleoprotein), thereby allowing to differentiate immunity after vaccination versus immunity after infection. …”
Publicado 2021
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5188por Boekel, Laura, Stalman, Eileen W, Wieske, Luuk, Hooijberg, Femke, van Dam, Koos P J, Besten, Yaëlle R, Kummer, Laura Y L, Steenhuis, Maurice, van Kempen, Zoé L E, Killestein, Joep, Volkers, Adriaan G, Tas, Sander W, van der Kooi, Anneke J, Raaphorst, Joost, Löwenberg, Mark, Takkenberg, R Bart, D'Haens, Geert R A M, Spuls, Phyllis I, Bekkenk, Marcel W, Musters, Annelie H, Post, Nicoline F, Bosma, Angela L, Hilhorst, Marc L, Vegting, Yosta, Bemelman, Frederike J, Voskuyl, Alexandre E, Broens, Bo, Parra Sanchez, Agner, van Els, Cécile A C M, de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J G M, Ruiter, Annabel M, van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Cornelia F, Teng, Y K Onno, van Paassen, Pieter, Busch, Matthias H, Jallah, Papay B P, Brusse, Esther, van Doorn, Pieter A, Baars, Adája E, Hijnen, Dirk Jan, Schreurs, Corine R G, van der Pol, W Ludo, Goedee, H Stephan, Vogelzang, Erik H, Leeuw, Maureen, Atiqi, Sadaf, van Vollenhoven, Ronald, Gerritsen, Martijn, van der Horst-Bruinsma, Irene E, Lems, Willem F, Nurmohamed, Mike T, Boers, Maarten, Keijzer, Sofie, Keijser, Jim, van de Sandt, Carolien, Boogaard, Arend, Cristianawati, Olvi, ten Brinke, Anja, Verstegen, Niels J M, Zwinderman, Koos A H, van Ham, S Marieke, Rispens, Theo, Kuijpers, Taco W, Wolbink, Gertjan, Eftimov, Filip“…We analysed post-vaccination serum samples for anti-receptor binding domain (RBD) antibodies to assess the humoral vaccination response (T2B! …”
Publicado 2022
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5189por Ciccone, Emily, Zhu, Deanna, Hawke, Samuel, Ajeen, Rawan, Gunderson, Annika, Lodge, Evans, Shook-Sa, Bonnie E, Abernathy, Haley, Garrett, Haley, King, Elise, Markmann, Alena, Premkumar, Lakshmanane, Juliano, Jonathan J, Boyce, Ross M, Aiello, Allison“…ELISA measured total immunoglobulin (Ig) and IgG binding to SARS-CoV-2 RBD. Neutralization was measured by live virus Nanoluc SARS-CoV-2ic assay. …”
Publicado 2022
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5190por Terpos, Evangelos, Politou, Marianna, Sergentanis, Theodoros N, Mentis, Andreas, Pappa, Vassiliki, Pagoni, Maria, Grouzi, Elisavet, Labropoulou, Stavroula, Charitaki, Ioanna, Moschandreou, Dimitra, Bouhla, Anthi, Saridakis, Stylianos, Korompoki, Eleni, Giatra, Chara, Bagratuni, Tina, Pefanis, Angelos, Papageorgiou, Sotirios, Spyridonidis, Alexandros, Antoniadou, Anastasia, Kotanidou, Anastasia, Syrigos, Konstantinos, Stamoulis, Konstantinos, Panayiotakopoulos, George, Tsiodras, Sotirios, Alexopoulos, Leonidas, Dimopoulos, Meletios A“…For the detection of anti-SARS-CoV-2 antibodies we used two commercially developed assays: one ELISA assay (Euroimmun Medizinische Labordiagnostika AG, Lubeck, Germany), which detects antibodies against the recombinant Spike protein of the virus (S1 domain) and a multiplex assay (ProtATonce Ltd, Athens, Greece) based on the Luminex® xMAP™ technology that detects total antibodies (IgG/IgM/IgA) and individual antibody isotypes IgG, IgM and IgA against 3 SARS-CoV-2 antigens (S1, basic nucleocapsid (N) protein and receptor-binding domain (RBD). Results: To-date, 260 (137M/123F) possible plasma donors were tested for the presence of anti-SARS-CoV-2 antibodies. …”
Publicado 2020
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5191por Ghandili, Susanne, Schönlein, Martin, Becher, Heiko, Wiessner, Christian, Lütgehetmann, Marc, Schulze zur Wiesch, Julian, Bokemeyer, Carsten, Sinn, Marianne, Leypoldt, Lisa, Weisel, Katja“…Samples were analyzed for the presence of SARS-CoV-2 specific antibodies using the quantitative anti-spike IgG (SARS-CoV-2 spike RBD IgG, cut off ≥ 0.8 BAU/ml) according to manufacturer's recommendations. …”
Publicado 2021
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