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1501por Patiño-Galindo, Juan Ángel, Filip, Ioan, Chowdhury, Ratul, Maranas, Costas D., Sorger, Peter K., AlQuraishi, Mohammed, Rabadan, Raul“…Here, we find evidence of a recombination event in the RBD involving ancestral linages to both SARS-CoV and SARS-CoV-2. …”
Publicado 2021
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1502por Byrnes, James R., Zhou, Xin X., Lui, Irene, Elledge, Susanna K., Glasgow, Jeff E., Lim, Shion A., Loudermilk, Rita P., Chiu, Charles Y., Wang, Taia T., Wilson, Michael R., Leung, Kevin K., Wells, James A.“…In this approach based on the use of enzyme-linked immunosorbent assays (ELISA), we present natively folded viral Spike protein receptor-binding domain (RBD)-containing antigens via avidin-biotin interactions. …”
Publicado 2020
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1503por Nguyen-Contant, Phuong, Embong, A. Karim, Kanagaiah, Preshetha, Chaves, Francisco A., Yang, Hongmei, Branche, Angela R., Topham, David J., Sangster, Mark Y.“…Convalescent subjects had high levels of IgG against the RBD, S2, and N, together with large populations of RBD- and S2-reactive IgG MBCs. …”
Publicado 2020
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1504“…Subtle changes at the interface with ACE2 seem to be responsible for the enhanced affinity for the receptor of the SARS-CoV-2 RBD compared to SARS-CoV RBD. Here, we use Elastic Network Models (ENMs) to study the response of the viral RBDs and ACE2 upon dissassembly of the complexes. …”
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1505por Padhi, Aditya K., Seal, Aniruddha, Khan, Javed Masood, Ahamed, Maqusood, Tripathi, Timir“…Molecular dynamics (MD) simulation-based analyses demonstrate that arbidol binds and stabilizes at the receptor-binding domain (RBD)/ACE2 interface with a high affinity. It forms stronger intermolecular interactions with the RBD than ACE2. …”
Publicado 2021
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1506por Hattori, Takamitsu, Koide, Akiko, Noval, Maria G., Panchenko, Tatyana, Romero, Larizbeth A., Teng, Kai Wen, Tada, Takuya, Landau, Nathaniel R., Stapleford, Kenneth A., Koide, Shohei“…Consequently, antibodies targeting the ACE2-interacting surface (ACE2IS) located in the receptor-binding domain (RBD) of the spike protein can neutralize the virus. …”
Publicado 2021
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1507A single-dose mRNA vaccine provides a long-term protection for hACE2 transgenic mice from SARS-CoV-2por Huang, Qingrui, Ji, Kai, Tian, Siyu, Wang, Fengze, Huang, Baoying, Tong, Zhou, Tan, Shuguang, Hao, Junfeng, Wang, Qihui, Tan, Wenjie, Gao, George F., Yan, Jinghua“…Here, we developed a nucleoside-modified mRNA vaccine in lipid-encapsulated form that encoded the SARS-CoV-2 RBD, termed as mRNA-RBD. A single immunization of mRNA-RBD elicited both robust neutralizing antibody and cellular responses, and conferred a near-complete protection against wild SARS-CoV-2 infection in the lungs of hACE2 transgenic mice. …”
Publicado 2021
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1508“…The incidence of metabolic syndrome was lowered in the order of RBD, WSD, and KBD. The participants having a KBD had lower serum C-reactive protein and triglyceride concentrations than those with RBD and WSD (p < 0.05). …”
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1509por Whitcombe, Alana L, McGregor, Reuben, Craigie, Alyson, James, Alex, Charlewood, Richard, Lorenz, Natalie, Dickson, James MJ, Sheen, Campbell R, Koch, Barbara, Fox‐Lewis, Shivani, McAuliffe, Gary, Roberts, Sally A, Morpeth, Susan C, Taylor, Susan, Webb, Rachel H, Jack, Susan, Upton, Arlo, Ussher, James E, Moreland, Nicole J“…Anti‐RBD antibodies were strongly correlated with NAbs at all time points (Pearson's r ≥ 0.87), and feasibility of using finger prick sampling to accurately measure anti‐RBD IgG was demonstrated. …”
Publicado 2021
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1510por Vishwakarma, Preeti, Yadav, Naveen, Rizvi, Zaigham Abbas, Khan, Naseem Ahmed, Chiranjivi, Adarsh Kumar, Mani, Shailendra, Bansal, Manish, Dwivedi, Prabhanjan, Shrivastava, Tripti, Kumar, Rajesh, Awasthi, Amit, Ahmed, Shubbir, Samal, Sweety“…The S1 subunit harbors the N-terminal domain (NTD) and the receptor-binding domain (RBD). The RBD is responsible for binding to host-cellular receptor angiotensin-converting enzyme 2 (ACE2). …”
Publicado 2021
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1511“…Methods: Primary bovine alveolar macrophages (PBAMs), RAW264.7 mouse macrophages, and THP-1 human cells were transfected with plasmids carrying the genes encoding the SARS-CoV-2 spike (S) protein or receptor-binding domain (RBD). Proteins in the macrophages interacting with S-RBD at 39.5 °C or 37 °C were identified by immunoprecipitation-mass spectrometry. …”
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1512por Li, Cesheng, Yu, Ding, Wu, Xiao, Liang, Hong, Zhou, Zhijun, Xie, Yong, Li, Taojing, Wu, Junzheng, Lu, Fengping, Feng, Lu, Mao, Min, Lin, Lianzhen, Guo, Huanhuan, Yue, Shenglan, Wang, Feifei, Peng, Yan, Hu, Yong, Wang, Zejun, Yu, Jianhong, Zhang, Yong, Lu, Jia, Ning, Haoran, Yang, Huichuan, Fu, Daoxing, He, Yanlin, Zhou, Dongbo, Du, Tao, Duan, Kai, Dong, Demei, Deng, Kun, Zou, Xia, Zhang, Ya, Zhou, Rong, Gao, Yang, Zhang, Xinxin, Yang, Xiaoming“…Moreover, male plasma donors produce more RBD-IgG than female, and age of the patients positively correlates with the RBD-IgG titer. …”
Publicado 2021
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1513por Wisnewski, Adam V., Liu, Jian, Lucas, Carolina, Klein, Jon, Iwasaki, Akiko, Cantley, Linda, Fazen, Louis, Campillo Luna, Julian, Slade, Martin, Redlich, Carrie A.“…We hypothesized that competitive enzyme linked immunoassays (ELISAs) based on SARS-CoV-2 spike protein’s receptor binding domain (RBD) attachment to its host receptor, the angiotensin converting enzyme 2 receptor (ACE2r), would correlate with PRNT, given the central role of RBD-ACE2r interactions in infection and published studies to date, and enable evaluation of vaccine responses. …”
Publicado 2022
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1514por Jiang, Linglei, Driedonks, Tom A.P., Jong, Wouter S.P., Dhakal, Santosh, van den Berg van Saparoea, H. Bart, Sitaras, Ioannis, Zhou, Ruifeng, Caputo, Christopher, Littlefield, Kirsten, Lowman, Maggie, Chen, Mengfei, Lima, Gabriela, Gololobova, Olesia, Smith, Barbara, Mahairaki, Vasiliki, Richardson, M. Riley, Mulka, Kathleen R., Lane, Andrew P., Klein, Sabra L., Pekosz, Andrew, Brayton, Cory F., Mankowski, Joseph L., Luirink, Joen, Villano, Jason S., Witwer, Kenneth W.“…Here, we present a novel, well-characterized SARS-CoV-2 vaccine candidate based on extracellular vesicles (EVs) of Salmonella typhimurium that are decorated with the mammalian cell culture-derived Spike receptor-binding domain (RBD). RBD-conjugated outer membrane vesicles (RBD-OMVs) were used to immunize the golden Syrian hamster (Mesocricetus auratus) model of COVID-19. …”
Publicado 2022
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1515por Wang, Yifan, Liu, Caixuan, Zhang, Chao, Wang, Yanxing, Hong, Qin, Xu, Shiqi, Li, Zuyang, Yang, Yong, Huang, Zhong, Cong, Yao“…Our cryo-EM analysis suggests that RBD destabilizations lead to population shift towards the more RBD-up and S1 destabilized fusion-prone state, beneficial for engagement with ACE2 and shedding of S1. …”
Publicado 2022
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1516por Jiang, Linglei, Driedonks, Tom A.P., Jong, Wouter S.P., Dhakal, Santosh, Bart van den Berg van Saparoea, H., Sitaras, Ioannis, Zhou, Ruifeng, Caputo, Christopher, Littlefield, Kirsten, Lowman, Maggie, Chen, Mengfei, Lima, Gabriela, Gololobova, Olesia, Smith, Barbara, Mahairaki, Vasiliki, Riley Richardson, M., Mulka, Kathleen R., Lane, Andrew P., Klein, Sabra L., Pekosz, Andrew, Brayton, Cory, Mankowski, Joseph L., Luirink, Joen, Villano, Jason S., Witwer, Kenneth W.“…Here, we present a novel, well‐characterized SARS‐CoV‐2 vaccine candidate based on extracellular vesicles (EVs) of Salmonella typhimurium that are decorated with the mammalian cell culture‐derived Spike receptor‐binding domain (RBD). RBD‐conjugated outer membrane vesicles (RBD‐OMVs) were used to immunize the golden Syrian hamster (Mesocricetus auratus) model of COVID‐19. …”
Publicado 2022
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1517por Richardson, Jennifer R., Götz, Ralph, Mayr, Vanessa, Lohse, Martin J., Holthoff, Hans-Peter, Ungerer, Martin“…METHODS AND RESULTS: In 23 vaccinated subjects, we documented high anti-SARS-CoV2 spike protein receptor binding domain (RBD) antibody titers. Average virus neutralization by antibodies, assessed as inhibition of ACE2 binding to RBD, was 2.2-fold reduced for delta mutant vs. wild type (wt) RBD. …”
Publicado 2022
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1518por Bauer, Magnus S., Gruber, Sophia, Hausch, Adina, Gomes, Priscila S. F. C., Milles, Lukas F., Nicolaus, Thomas, Schendel, Leonard C., Navajas, Pilar López, Procko, Erik, Lietha, Daniel, Melo, Marcelo C. R., Bernardi, Rafael C., Gaub, Hermann E., Lipfert, Jan“…Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections are initiated by attachment of the receptor-binding domain (RBD) on the viral Spike protein to angiotensin-converting enzyme-2 (ACE2) on human host cells. …”
Publicado 2022
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1519por Xu, Zepeng, Kang, Xinrui, Han, Pu, Du, Pei, Li, Linjie, Zheng, Anqi, Deng, Chuxia, Qi, Jianxun, Zhao, Xin, Wang, Qihui, Liu, Kefang, Gao, George Fu“…We also determined the crystal structures of eqACE2/RaTG13-RBD, eqACE2/SARS-CoV-2 PT-RBD and eqACE2/Omicron BA.1-RBD. …”
Publicado 2022
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1520por Alhamo, Moyasar A., Boley, Patricia A., Liu, Mingde, Niu, Xiaoyu, Yadav, Kush Kumar, Lee, Carolyn, Saif, Linda J., Wang, Qiuhong, Kenney, Scott P.“…We used recombinant chimeric viruses, which express S protein or the RBD of SpCoV (icPDCoV-S(HKU17), and icPDCoV-RBD(ISU)) on the genomic backbone of an infectious clone of PDCoV (icPDCoV). …”
Publicado 2022
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