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1761por Behloul, Nouredine, Baha, Sarra, Guo, Yuqian, Yang, Zhifang, Shi, Ruihua, Meng, Jihong“…According to our screening and selection criteria, three approved antivirals (elbasvir, grazoprevir, and sovaprevir) and 4 other drugs (hesperidin, pamaqueside, diosmin, and sitogluside) were identified as potent binders of the RBD. The binding of these molecules involved several RBD residues required for the interaction of the virus with its cellular receptor. …”
Publicado 2021
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1762por Zhang, Pingping, Gao, Qi, Wang, Tang, Ke, Yuehua, Mo, Fei, Jia, Ruizhong, Liu, Wanbing, Liu, Lei, Zheng, Shangen, Liu, Yuzhen, Li, Luping, Wang, Yao, Xu, Lei, Hao, Kun, Min, Wei, Liu, Xiaoli, Yang, Ruifu, Li, Shiyue, Lin, Changqing, Zhao, Yong“…In this study, six recombinant nucleocapsid and spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were prepared and evaluated, including three prokaryotic expression nucleocapsid proteins (rN, rN1, rN2) and three eukaryotic expression spike proteins (rS1, rS-RBD, rS-RBD-mFc). The recombinant proteins with the highest ELISA titers (rS1 and rS-RBD-mFc) were selected to develop a double-antigen sandwich colloidal gold immunochromatography assay (GICA) to detect total antibodies against SARS-CoV-2. …”
Publicado 2020
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1763“…RECENT FINDINGS: The polysomnogram is fundamentally important in the evaluation of sleep-disordered breathing in the setting of cardiovascular comorbidities and neurologic conditions such as neuromuscular disease, stroke, and epilepsy and in the evaluation of dream enactment behavior in the setting of REM sleep behavior disorder (RBD). Because RBD is predictive of neurodegenerative disorders, recent data highlights the importance of PSG in corroborating the diagnosis of RBD and identifying people who may be at risk. …”
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1764por Chourasia, Rounak, Padhi, Srichandan, Chiring Phukon, Loreni, Abedin, Md Minhajul, Singh, Sudhir P., Rai, Amit Kumar“…In this study, search for potential inhibitors against two of the best characterized SARS-CoV-2 drug targets: S1 glycoprotein receptor-binding domain (RBD) and main protease (3CL(Pro)), was carried out using the soy cheese peptides. …”
Publicado 2020
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1765por Terpos, Evangelos, Politou, Marianna, Sergentanis, Theodoros N., Mentis, Andreas, Rosati, Margherita, Stellas, Dimitris, Bear, Jenifer, Hu, Xintao, Felber, Barbara K., Pappa, Vassiliki, Pagoni, Maria, Grouzi, Elisavet, Labropoulou, Stavroula, Charitaki, Ioanna, Ntanasis-Stathopoulos, Ioannis, Moschandreou, Dimitra, Bouhla, Anthi, Saridakis, Stylianos, Korompoki, Eleni, Giatra, Chara, Bagratuni, Tina, Pefanis, Angelos, Papageorgiou, Sotirios, Spyridonidis, Alexandros, Antoniadou, Anastasia, Kotanidou, Anastasia, Syrigos, Konstantinos, Stamoulis, Konstantinos, Panayiotakopoulos, George, Tsiodras, Sotirios, Alexopoulos, Leonidas, Dimopoulos, Meletios A., Pavlakis, George N.“…Different assays were used: a commercial ELISA detecting antibodies against the recombinant spike protein (S1); a multiplex assay detecting total and specific antibody isotypes against three SARS-CoV-2 antigens (S1, basic nucleocapsid (N) protein and receptor-binding domain (RBD)); and an in-house ELISA detecting antibodies to complete spike, RBD and N in 60 of these donors. …”
Publicado 2020
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1766por Xu, Cong, Wang, Yanxing, Liu, Caixuan, Zhang, Chao, Han, Wenyu, Hong, Xiaoyu, Wang, Yifan, Hong, Qin, Wang, Shutian, Zhao, Qiaoyu, Wang, Yalei, Yang, Yong, Chen, Kaijian, Zheng, Wei, Kong, Liangliang, Wang, Fangfang, Zuo, Qinyu, Huang, Zhong, Cong, Yao“…Accompanying ACE2 binding to the up receptor-binding domain (RBD), the associated ACE2-RBD exhibits continuous swing motions. …”
Publicado 2021
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1767por Klingler, Jéromine, Weiss, Svenja, Itri, Vincenza, Liu, Xiaomei, Oguntuyo, Kasopefoluwa Y, Stevens, Christian, Ikegame, Satoshi, Hung, Chuan-Tien, Enyindah-Asonye, Gospel, Amanat, Fatima, Baine, Ian, Arinsburg, Suzanne, Bandres, Juan C, Kojic, Erna Milunka, Stoever, Jonathan, Jurczyszak, Denise, Bermudez-Gonzalez, Maria, Nádas, Arthur, Liu, Sean, Lee, Benhur, Zolla-Pazner, Susan, Hioe, Catarina E“…Virus infection requires the receptor-binding domain (RBD) of the spike protein. Although studies have demonstrated anti-spike and -RBD antibodies to be protective in animal models, and convalescent plasma as a promising therapeutic option, little is known about immunoglobulin isotypes capable of blocking infection. …”
Publicado 2020
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1768por Röltgen, Katharina, Powell, Abigail E., Wirz, Oliver F., Stevens, Bryan A., Hogan, Catherine A., Najeeb, Javaria, Hunter, Molly, Wang, Hannah, Sahoo, Malaya K., Huang, ChunHong, Yamamoto, Fumiko, Manohar, Monali, Manalac, Justin, Otrelo-Cardoso, Ana R., Pham, Tho D., Rustagi, Arjun, Rogers, Angela J., Shah, Nigam H., Blish, Catherine A., Cochran, Jennifer R., Jardetzky, Theodore S., Zehnder, James L., Wang, Taia T., Narasimhan, Balasubramanian, Gombar, Saurabh, Tibshirani, Robert, Nadeau, Kari C., Kim, Peter S., Pinsky, Benjamin A., Boyd, Scott D.“…SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, can neutralize the virus. …”
Publicado 2020
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1769“…Herein, we developed a novel fluorescence resonance energy transfer (FRET)-based hACE2 biosensor to monitor the interaction between hACE2 and SARS-CoV-2 RBD. This biosensor facilitated the visualization of hACE2-RBD activity with high spatiotemporal resolutions at the single-cell level. …”
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1770por Nayak, Kaustuv, Gottimukkala, Kamalvishnu, Kumar, Sanjeev, Reddy, Elluri Seetharami, Edara, Venkata Viswanadh, Kauffman, Robert, Floyd, Katharine, Mantus, Grace, Savargaonkar, Deepali, Goel, Pawan Kumar, Arora, Satyam, Rahi, Manju, Davis, Carl W., Linderman, Susanne, Wrammert, Jens, Suthar, Mehul S., Ahmed, Rafi, Sharma, Amit, Murali-Krishna, Kaja, Chandele, Anmol“…RBD-specific IgG, but not IgA or IgM titers, correlated with neutralizing antibody titers and RBD-specific memory B cell frequencies. …”
Publicado 2021
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1771por Wang, Yaling, Xie, Yuping, Luo, Jia, Guo, Mengyu, Hu, Xuhao, Chen, Xi, Chen, Ziwei, Lu, Xinyi, Mao, Lichun, Zhang, Kai, Wei, Liangnian, Ma, Yunfei, Wang, Ruixin, Zhou, Jia, He, Chunyan, Zhang, Yufang, Zhang, Ye, Chen, Sisi, Shen, Lijuan, Chen, Yun, Qiu, Nasha, Liu, Ying, Cui, Yanyan, Liao, Guoyang, Liu, Ye, Chen, Chunying“…Notably, even at a 5-fold lower antigen dose and with fewer injections, the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (~270-fold) and live coronavirus (>8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DCs, CD4(+) and CD8(+) T lymphocytes. …”
Publicado 2021
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1772por He, Linling, Lin, Xiaohe, Wang, Ying, Abraham, Ciril, Sou, Cindy, Ngo, Timothy, Zhang, Yi, Wilson, Ian A., Zhu, Jiang“…An antibody column specific for the RBD enabled tag-free vaccine purification. In mice, the 24-meric RBD-ferritin SApNP elicited a more potent neutralizing antibody (NAb) response than the RBD alone and the spike with two stabilizing proline mutations in S2 (S2P). …”
Publicado 2021
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1773por Fotis, Christos, Meimetis, Nikolaos, Tsolakos, Nikos, Politou, Marianna, Akinosoglou, Karolina, Pliaka, Vaia, Minia, Angeliki, Terpos, Evangelos, Trougakos, Ioannis P., Mentis, Andreas, Marangos, Markos, Panayiotakopoulos, George, Dimopoulos, Meletios A., Gogos, Charalampos, Spyridonidis, Alexandros, Alexopoulos, Leonidas G.“…A multi-antigen approach requiring partial agreement between RBD and N or S1 readouts (RBD&N|S1 rule) was less affected by cut-off selection, resulting in robust seroprevalence estimation [0.6% (95% CI 0.3–1.1%)–1.2% (95% CI 0.7–2.0%)] and accurate identification of seroconverted individuals.…”
Publicado 2021
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1774por Van Egeren, Debra, Novokhodko, Alexander, Stoddard, Madison, Tran, Uyen, Zetter, Bruce, Rogers, Michael, Pentelute, Bradley L., Carlson, Jonathan M., Hixon, Mark, Joseph-McCarthy, Diane, Chakravarty, Arijit“…The spike protein receptor-binding domain (RBD) of SARS-CoV-2 is the molecular target for many vaccines and antibody-based prophylactics aimed at bringing COVID-19 under control. …”
Publicado 2021
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1775por Mishra, Ganesh Prasad, Bhadane, Rajendra N., Panigrahi, Debadash, Amawi, Haneen A., Asbhy, Charles R., Tiwari, Amit K.“…The five important targets chosen were the main protease (Mpro), Spike receptor binding domain (Spike-RBD), RNA - dependent RNA polymerase (RdRp or Nsp12), non-structural protein 15 (Nsp15) of SARS-CoV-2 and the host angiotensin converting enzyme-2 (ACE-2) spike-RBD binding domain. …”
Publicado 2021
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1776por Jalkanen, Pinja, Pasternack, Arja, Maljanen, Sari, Melén, Krister, Kolehmainen, Pekka, Huttunen, Moona, Lundberg, Rickard, Tripathi, Lav, Khan, Hira, Ritvos, Mikael A, Naves, Rauno, Haveri, Anu, Österlund, Pamela, Kuivanen, Suvi, Jääskeläinen, Anne J, Kurkela, Satu, Lappalainen, Maija, Rantasärkkä, Kaisa, Vuorinen, Tytti, Hytönen, Jukka, Waris, Matti, Tauriainen, Sisko, Ritvos, Olli, Kakkola, Laura, Julkunen, Ilkka“…SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. S1 and RBD-based EIA results had a strong correlation with microneutralization test results. …”
Publicado 2021
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1777por Dileepan, Mythili, Di, Da, Huang, Qinfeng, Ahmed, Shamim, Heinrich, Daniel, Ly, Hinh, Liang, Yuying“…The average seroprevalence of N- and RBD-specific antibodies in pet cats were 8% and 3%, respectively. …”
Publicado 2021
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1778por Prabhakara, Chaitra, Godbole, Rashmi, Sil, Parijat, Jahnavi, Sowmya, Gulzar, Shah-e-Jahan, van Zanten, Thomas S., Sheth, Dhruv, Subhash, Neeraja, Chandra, Anchal, Shivaraj, Akshatha, Panikulam, Patricia, U, Ibrahim, Nuthakki, Vijay Kumar, Puthiyapurayil, Theja Parassini, Ahmed, Riyaz, Najar, Ashaq Hussain, Lingamallu, Sai Manoz, Das, Snigdhadev, Mahajan, Bhagyashri, Vemula, Praveen, Bharate, Sandip B., Singh, Parvinder Pal, Vishwakarma, Ram, Guha, Arjun, Sundaramurthy, Varadharajan, Mayor, Satyajit“…Ectopic expression of ACE2 (AGS-ACE2) results in RBD traffic via both CG and clathrin-mediated endocytosis. …”
Publicado 2021
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1779por Li, Jiang-Fan, He, Lei, Deng, Yong-Qiang, Qi, Shu-Hui, Chen, Yue-Hong, Zhang, Xiao-Lu, Hu, Shi-Xiong, Fan, Rui-Wen, Zhao, Guang-Yu, Qin, Cheng-Feng“…In summary, we developed a novel nanobody targeting SARS-CoV RBD, which might be useful for the development of therapeutics against SARS.…”
Publicado 2021
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1780por Malipiero, Giacomo, Moratto, Anna, Infantino, Maria, D’Agaro, Pierlanfranco, Piscianz, Elisa, Manfredi, Mariangela, Grossi, Valentina, Benvenuti, Enrico, Bulgaresi, Matteo, Benucci, Maurizio, Villalta, Danilo“…On the contrary, a weak but significant positive correlation was found between INFγ and anti-RBD Abs values (rho = 0.354, p = 0.003). As to the autoimmune cohort, anti-RBD Abs were not detected in the two patients with absent peripheral CD19(+)B cells, despite high INFγ levels being observed in all 5 patients after vaccination. …”
Publicado 2021
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