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1781por Camacho-Sandoval, Rosa, Nieto-Patlán, Alejandro, Carballo-Uicab, Gregorio, Montes-Luna, Alejandra, Jiménez-Martínez, María C., Vallejo-Castillo, Luis, González-González, Edith, Arrieta-Oliva, Hugo Iván, Gómez-Castellano, Keyla, Guzmán-Bringas, Omar U., Cruz-Domínguez, María Pilar, Medina, Gabriela, Montiel-Cervantes, Laura A., Gordillo-Marín, Maricela, Vázquez-Campuzano, Roberto, Torres-Longoria, Belem, López-Martínez, Irma, Pérez-Tapia, Sonia M., Almagro, Juan Carlos“…The implementation and validation of anti-SARS-CoV-2 IgG serological assays are reported in this paper. S1 and RBD proteins were used to coat ELISA plates, and several secondary antibodies served as reporters. …”
Publicado 2021
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1782por Rahbar, Mohammad Reza, Jahangiri, Abolfazl, Khalili, Saeed, Zarei, Mahboubeh, Mehrabani-Zeinabad, Kamran, Khalesi, Bahman, Pourzardosht, Navid, Hessami, Anahita, Nezafat, Navid, Sadraei, Saman, Negahdaripour, Manica“…Therefore, their substitution may affect vaccine efficacy. Higher rate of RBD maintenance than furin cleavage site was predicted. …”
Publicado 2021
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1783por Feldman, Jared, Bals, Julia, Altomare, Clara G., St. Denis, Kerri, Lam, Evan C., Hauser, Blake M., Ronsard, Larance, Sangesland, Maya, Moreno, Thalia Bracamonte, Okonkwo, Vintus, Hartojo, Nathania, Balazs, Alejandro B., Bajic, Goran, Lingwood, Daniel, Schmidt, Aaron G.“…Here, we isolated naive B cells from 8 seronegative human donors targeting the SARS-CoV-2 receptor-binding domain (RBD). Single cell B cell receptor (BCR) sequencing identified diverse gene usage and no restriction on complementarity determining region length. …”
Publicado 2021
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1784por Bartsch, Yannic, Tong, Xin, Kang, Jaweon, José Avendaño, María, Serrano, Eileen F., García-Salum, Tamara, Pardo-Roa, Catalina, Riquelme, Arnoldo, Medina, Rafael A., Alter, Galit“…Compromised RBD binding IgG was accompanied by a significant loss of cross RBD-specific antibody Fcγ-receptor binding by the CoronaVac vaccine, but preservation of RBD-specific FcγR2a and Fcγ3a binding across the mRNA vaccines. …”
Publicado 2021
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1785por Jang, Tae-Ho, Park, Woo-Jung, Lee, Hansaem, Woo, Hye-Min, Lee, So-young, Kim, Kyung-Chang, Kim, Sung Soon, Hong, Eunmi, Song, Jaeyoung, Lee, Joo-Yeon“…Here, we provide a molecular method for neutralizing MERS-CoV through potent antibody-mediated targeting of the receptor-binding subdomain (RBD) of the spike protein. The structural characterization of the neutralizing antibody (KNIH90-F1) complexed with RBD using X-ray crystallography revealed three critical epitopes (D509, R511, and E513) in the RBD region of the spike protein. …”
Publicado 2022
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1786“…The protocol for the detection of BAbs could be utilized for detection of the RBD-N501Y variant with equal sensitivity and speed. …”
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1787por Yang, Qiangzhen, Jian, Xuemin, Syed, Ali Alamdar Shah, Fahira, Aamir, Zheng, Chenxiang, Zhu, Zijia, Wang, Ke, Zhang, Jinmai, Wen, Yanqin, Li, Zhiqiang, Pan, Dun, Lu, Tingting, Wang, Zhuo, Shi, Yongyong“…The analysis of the binding affinities of ACE2-RBD, antibody-NTD, and antibody-RBD complexes in the different variants revealed that the recognition of antibodies against S1 NTD and RBD was decreased in the variants, especially the Delta variant compared with the original strain, which may induce the immune evasion of SARS-CoV-2 variants. …”
Publicado 2022
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1788por Zou, Jia, Li, Li, Zheng, Peiyi, Liang, Wenhua, Hu, Siyi, Zhou, Shuaixiang, Wang, Yanqun, Zhao, Jincun, Yuan, Daopeng, Liu, Lu, Wu, Dongdong, Xu, Mengqiu, Zhang, Fangfang, Zhu, Mengzhu, Wu, Zhihai, Cao, Xiaochao, Ni, Meng, Ling, Xiaomin, Wu, Yue, Kuang, Zhihui, Hu, Moyan, Li, Jianfeng, Li, Xue, Guo, Xiling, Xu, Tianmin, Jiang, Haiping, Gao, Changshou, Yu, Michael, Liu, Junjian, Zhong, Nanshan, Zhou, Jianfeng, Huang, Jian-an, Jin, Tengchuan, He, Jianxing“…P15-16 has only 1 binding hotspot, which is K378 in the RBD of SARS-CoV-2. The crystal structure of the P5-22, P14-44, and RBD ternary complex clarified the unique mechanisms that underlie the excellent mutation resistance profiles of these antibodies. …”
Publicado 2022
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1789por Zhang, Fuming, He, Peng, Rodrigues, Andre L., Jeske, Walter, Tandon, Ritesh, Bates, John T., Bierdeman, Michael A., Fareed, Jawed, Dordick, Jonathan, Linhardt, Robert J.“…PPS and MPS showed the strongest inhibition of interaction of heparin and S-protein RBD. The competitive binding studies showed that the IC(50) of PPS and MPS against the S-protein RBD binding to immobilized heparin was ~35 nM and ~9 nM, respectively, much lower than the IC(50) for soluble heparin (IC(50) = 56 nM). …”
Publicado 2022
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1790por Kraus, Aurora, Huertas, Mar, Ellis, Laura, Boudinot, Pierre, Levraud, Jean-Pierre, Salinas, Irene“…Electrophysiological recordings reveal impaired olfactory function to both food and bile odorants in animals treated intranasally with SARS-CoV-2 S RBD. However, no loss of behavioral preference for food was detected in SARS-CoV-2 S RBD treated fish. …”
Publicado 2022
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1791por Forgacs, David, Moraes, Vanessa S., Hanley, Hannah B., Gattiker, Jasper L., Jefferson, Alexandria M., Ross, Ted M.“…Participants who received a third (booster) dose of an mRNA vaccine not only increased their anti-RBD antibody levels ~14-fold, but they also had ~3 times more anti-RBD antibodies compared to the peak of their antibody levels after receiving their primary vaccine series. …”
Publicado 2022
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1792por Charmetant, Xavier, Espi, Maxime, Benotmane, Ilies, Barateau, Véronique, Heibel, Francoise, Buron, Fanny, Gautier-Vargas, Gabriela, Delafosse, Marion, Perrin, Peggy, Koenig, Alice, Cognard, Noëlle, Levi, Charlène, Gallais, Floriane, Manière, Louis, Rossolillo, Paola, Soulier, Eric, Pierre, Florian, Ovize, Anne, Morelon, Emmanuel, Defrance, Thierry, Fafi-Kremer, Samira, Caillard, Sophie, Thaunat, Olivier“…Neutralizing anti-Receptor Binding Domain (RBD) IgG antibodies were identified as the primary correlate of protection for transplant recipients. …”
Publicado 2022
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1793por Hendriks, Jan, Schasfoort, Richard, Koerselman, Michelle, Dannenberg, Maureen, Cornet, Alexander Daniel, Beishuizen, Albertus, van der Palen, Job, Krabbe, Johannes, Mulder, Alide H. L., Karperien, Marcel“…Furthermore, we studied the interactions of the specific receptor-binding domain (RBD) in more depth by characterizing specific antibody response to a peptide library. …”
Publicado 2022
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1794por Lee, Beomki, Ko, Jae-Hoon, Park, Jiho, Moon, Hee-Won, Baek, Jin Yang, Jung, Sunhee, Lim, Hee-Young, Kim, Kyung-Chang, Huh, Kyungmin, Cho, Sun Young, Kang, Cheol-In, Chung, Doo Ryeon, Huh, Hee Jae, Chung, Chi Ryang, Kim, Yae-Jean, Joo, Eun-Jeong, Kang, Eun-Suk, Peck, Kyong Ran“…We measured the total antibodies for the receptor-binding domain (RBD) of the spike protein, total antibodies for the nucleocapsid protein (NP), and IgG antibodies for the RBD. …”
Publicado 2022
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1795por Casasnovas, José M., Margolles, Yago, Noriega, María A., Guzmán, María, Arranz, Rocío, Melero, Roberto, Casanova, Mercedes, Corbera, Juan Alberto, Jiménez-de-Oya, Nereida, Gastaminza, Pablo, Garaigorta, Urtzi, Saiz, Juan Carlos, Martín-Acebes, Miguel Ángel, Fernández, Luis Ángel“…Nbs and derived hcAbs bound with subnanomolar or picomolar affinities to the S and its RBD, and S-binding cross-competition clustered them in two different groups. …”
Publicado 2022
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1796por Liu, Liqin, Chen, Tingting, Zhou, Lizhi, Sun, Jie, Li, Yuqian, Nie, Meifeng, Xiong, Hualong, Zhu, Yuhe, Xue, Wenhui, Wu, Yangtao, Li, Tingting, Zhang, Tianying, Kong, Zhibo, Yu, Hai, Zhang, Jun, Gu, Ying, Zheng, Qingbing, Zhao, Qinjian, Xia, Ningshao, Li, Shaowei“…To this end, here, we used the Escherichia coli expression system to produce a recombinant fusion protein comprising the receptor binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; residues 319–541) and the fragment A domain of Cross-Reacting Material 197 (CRM197); hereafter, CRMA-RBD. …”
Publicado 2022
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1797por Yang, Jing, Lin, Sheng, Sun, Honglu, Chen, Zimin, Yang, Fanli, Lin, Xi, Guo, Liyan, Wang, Lingling, Wen, Ao, Zhang, Xindan, Dai, Yushan, He, Bin, Cao, Yu, Dong, Haohao, Liu, Xianbo, Chen, Bo, Li, Jian, Zhao, Qi, Lu, Guangwen“…In this study, we report the identification and characterization of a potent SARS-CoV-2 neutralizing nanobody that targets the viral spike receptor-binding domain (S-RBD). The nanobody, termed as Nb-007, engages SARS-CoV-2 S-RBD with the two-digit picomolar binding affinity and shows outstanding virus entry-inhibition activity. …”
Publicado 2022
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1798por Adam, Awadalkareem, Shi, Qing, Wang, Binbin, Zou, Jing, Mai, Junhua, Osman, Samantha R., Wu, Wenzhe, Xie, Xuping, Aguilar, Patricia V, Bao, Xiaoyong, Shi, Pei-Yong, Shen, Haifa, Wang, Tian“…Notably, mPSM facilitated the uptake of SARS-CoV-2 RBD antigens by nasal and airway epithelial cells. …”
Publicado 2022
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1799por Pratesi, Federico, Errante, Fosca, Pacini, Lorenzo, Peña-Moreno, Irina Charlot, Quiceno, Sebastian, Carotenuto, Alfonso, Balam, Saidou, Konaté, Drissa, Diakité, Mahamadou M., Arévalo-Herrera, Myriam, Kajava, Andrey V., Rovero, Paolo, Corradin, Giampietro, Migliorini, Paola, Papini, Anna M., Herrera, Sócrates“…Based on the analysis of available data, we selected a 72 aa long receptor binding motif (RBM(436-507)) of RBD. We used ELISA to study the antibody response to each of the three antigens (S protein, its RBD domain and the RBM(436-507) synthetic peptide) in humans exposed to the infection and in immunized mice. …”
Publicado 2022
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1800“…In this study, we have mapped and reported mutations on the modelled RBD and evaluated binding affinities of various human antibodies with it. …”
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