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41“…We give an overview of the achievements of paleolimnology and the reconstruction of environmental history of lakes, review the untapped diversity of aquatic organisms that produce dormant propagules, compare Daphnia as a model of resurrection ecology with other organisms amenable to resurrection studies, especially diatoms, and consider new research directions that represent the nexus of these two fields.…”
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42“…Here, an SOP is described that provides a step-by-step protocol on the practice of resurrecting dormant Daphnia magna eggs. Two key studies are provided in which the fitness response of resurrected Daphnia magna populations to warming is measured, capitalizing on the ability to study historical and modern populations in the same settings. …”
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45por Kim, Jong S.Enlace del recurso
Publicado 2022
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48por Levin, Geoffrey A.Enlace del recurso
Publicado 2022
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51“…Using a transgenic approach, it was possible to resurrect DNA function in transgenic mice. The results demonstrate that the thylacine Col2A1 enhancer directed chondrocyte-specific expression in this extinct mammalian species in the same way as its orthologue does in mice. …”
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52por Perez-Jimenez, Raul, Inglés-Prieto, Alvaro, Zhao, Zi-Ming, Sanchez-Romero, Inmaculada, Alegre-Cebollada, Jorge, Kosuri, Pallav, Garcia-Manyes, Sergi, Kappock, T. Joseph, Tanokura, Masaru, Holmgren, Arne, Sanchez-Ruiz, Jose M., Gaucher, Eric A., Fernandez, Julio M.Enlace del recurso
Publicado 2011
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54por Howard, Conor J, Hanson-Smith, Victor, Kennedy, Kristopher J, Miller, Chad J, Lou, Hua Jane, Johnson, Alexander D, Turk, Benjamin E, Holt, Liam J“…Within this group, the cyclin dependent kinases (CDKs) and mitogen activated protein kinases (MAPKs) require proline at the +1 position of their substrates, while Ime2 prefers arginine. The resurrected common ancestor of CDKs, MAPKs, and Ime2 could phosphorylate substrates with +1 proline or arginine, with preference for proline. …”
Publicado 2014
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55“…The spirally arranged stems of the spikemoss Selaginella lepidophylla, an ancient resurrection plant, compactly curl into a nest-ball shape upon dehydration. …”
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56“…Curcumin mediates several processes like restoration of CD4(+)/CD8(+) T cell populations, reversal of type-2 cytokine bias, reduction of Treg cell population and suppression of T cell apoptosis; all these help to resurrect tumor immune surveillance that leads to tumor regression. …”
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58por Alcalde, Miguel“…The directed evolution of ancestral ‐resurrected‐ enzymes can give a new twist in protein engineering approaches towards more versatile and robust biocatalysts. …”
Publicado 2016
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59por Risso, Valeria A., Martinez-Rodriguez, Sergio, Candel, Adela M., Krüger, Dennis M., Pantoja-Uceda, David, Ortega-Muñoz, Mariano, Santoyo-Gonzalez, Francisco, Gaucher, Eric A., Kamerlin, Shina C. L., Bruix, Marta, Gavira, Jose A., Sanchez-Ruiz, Jose M.“…Mechanisms for the emergence of completely new active sites must therefore either plausibly exist or at least have existed at the primordial protein stage. Here, we use resurrected Precambrian proteins as scaffolds for protein engineering and demonstrate that a new active site can be generated through a single hydrophobic-to-ionizable amino acid replacement that generates a partially buried group with perturbed physico-chemical properties. …”
Publicado 2017
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60“…Configurations of ancient sequences can be inferred from the diversity of extant sequences, and then resurrected in the laboratory to ascertain their biochemical attributes. …”
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