“…Our data demonstrate that alternative splicing of structured pre-mRNAs can be regulated by small molecules that directly bind to the RNA, which is reminiscent to an RNA riboswitch.…”
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“…This ncRNA contains a c-di-GMP riboswitch. We suggest it functions as an intracellular reservoir for c-di-GMP, playing a role in the rapid switch from AP to GP.…”
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“…This analysis was extended to the binding of the preQ1 metabolite to a preQ1-II riboswitch.…”
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“…Riboswitches are cis-acting regulatory elements broadly distributed in bacterial mRNAs that control a wide range of critical metabolic activities. …”
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“…Here, we use NMR spectroscopy to study the dynamic properties of a 12-nucleotide (nt) ssRNA tail derived from the prequeuosine riboswitch linked to the 3′ end of a 48-nt hairpin. …”
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“…We identified 1151 individual transcription start sites, multiple riboswitch-like RNA elements, and a global set of antisense RNAs and previously unrecognized trans-acting RNAs. …”
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“…Thus riboswitch sequences allow folding to occur independently of protein binding, while U4 should remain unfolded until bound by protein. …”
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“…We also characterize the cellular structures of three endogenously expressed non-coding RNAs: 5S rRNA, RNase P and the btuB riboswitch. Finally, a comparison between in-cell and in vitro folded RNA structures revealed remarkable similarities for synthetic RNAs, but significant differences for RNAs that participate in complex cellular interactions. …”
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“…In a new approach, we have observed the competition between Mg(2+) and putrescine(2+) (in a background of K(+)) with native, partially unfolded and highly extended conformations of an adenine riboswitch aptamer. With the native state, putrescine(2+) is a weak competitor when the ratio of the excess Mg(2+) (which neutralizes phosphate charge) to RNA is very low, but becomes much more effective at replacing Mg(2+) as the excess Mg(2+) in the RNA ion atmosphere increases. …”
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“…To overcome the genetic instability, we created an inducible PopZ(At) strain by replacing the native ribosome binding site with a riboswitch. Cultivated in a medium without the inducer theophylline, the cells look like ΔpopZ(At) cells, with a branching and minicell phenotype. …”
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“…We have also discovered potential novel functional motifs conserved in ribosomal RNA, sgRNA, SRP RNA, riboswitch and ribozyme.…”
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“…The tetracycline-binding RNA aptamer (TC-aptamer) is a synthetic riboswitch that binds the antibiotic tetracycline (TC) with exceptionally high affinity. …”
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“…RNA switches include bacterial riboswitches, where ligand binding can stabilize a bound structure. …”
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“…We report the development of an RNA-array assay to perform such measurements and its application to a model RNA: the core glmS ribozyme riboswitch, which performs a ligand-dependent self-cleavage reaction. …”
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“…Taking advantage of the sole reported riboswitch metabolite sensor in plants, we show that TDP oscillates in the nucleus. …”
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“…Here, we demonstrate robust and tunable performance of a transcriptionally regulated, cell-free riboswitch encapsulated in lipid membranes, allowing the detection of fluoride, an environmentally important molecule. …”
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“…We demonstrate this principle utilizing a bacterial riboswitch that binds cyclic-di-guanosine monophosphate. …”
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“…Twelve non-coding RNAs were identified, including 11 small RNAs and one cobalamin riboswitch. Fifty-seven genes were differentially regulated in the LuxS mutant. …”
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“…This method provides a simple and informative tool for studies of regulatory sequences in RNA, in particular addressing RNA structure–function relationships (e.g. sRNA-mediated control, riboswitch elements). However, slight protocol modifications also permit mapping of functional DNA elements and functionally important regions in proteins.…”
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“…The riboSNitch is analogous to a riboswitch, where binding of a small molecule rather than mutation alters the structure of the RNA to control gene regulation. …”
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