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1081por Chen, Yng-Tay, Lin, Wei-De, Liao, Wen-Ling, Tsai, Ya-Ching, Liao, Jiunn-Wang, Tsai, Fuu-Jen“…Transient transfection NT5C2 into RIN-m5F cells down-regulated DNA methyltransferase I (DNMT1) expression and up-regulation of the insulin receptor. …”
Publicado 2020
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1082por Vennapusa, Amaranatha R., Somayanda, Impa M., Doherty, Colleen J., Jagadish, S. V. Krishna“…The absence of starch co-precipitation during RNA extraction resulted in enhanced yield and quality of RNA with RIN values of 7–9, quantified using a bioanalyzer. …”
Publicado 2020
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1083“…The expression levels of key ethylene biosynthesis genes (SlACS2, SlACS4, SlACO1, and SlACO3) and crucial ripening regulators (RIN and NOR) were increased in SlGRAS4-OE fruit. …”
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1084por Feng, Jihua, Pang, Jielong, He, Dan, Wu, Zimeng, Li, Qian, Ji, Pan, He, Cuiying, Zhong, Zhimei, Li, Hongyuan, Zhang, Jianfeng“…Hub genes included RETN, MVP, DEFA4, CTSG, AZU1, FMNL1, RBBP7, POLD4, RIN3, IRF7. ROC curve analysis of the hub gene GSVA index showed good diagnostic ability in sepsis or ARDS. …”
Publicado 2021
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1085por Ntamo, Yonela, Samodien, Ebrahim, Burger, Joleen, Muller, Nolan, Muller, Christo J. F., Chellan, Nireshni“…Over the years, immortalized rodent β-cell lines such as RIN, HIT, MIN, βTC, and INS-1 have been used to investigate pancreatic β-cell physiology using conventional two-dimensional (2D) culture techniques. …”
Publicado 2021
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1086por Montiel, Jesús, Reid, Dugald, Grønbæk, Thomas H, Benfeldt, Caroline M, James, Euan K, Ott, Thomas, Ditengou, Franck A, Nadzieja, Marcin, Kelly, Simon, Stougaard, Jens“…Inoculation of L. japonicus mutants shows that Ethylene-responsive factor required for nodulation 1 (Ern1) and Leu-rich Repeat Receptor-Like Kinase (RinRK1) are dispensable for intercellular infection in contrast to intracellular infection. …”
Publicado 2020
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1087por Martinez-Castillero, Maria, Then, Carlos, Altarriba, Juan, Srihi, Houssemeddine, López-Carbonell, David, Díaz, Clara, Martinez, Paulino, Hermida, Miguel, Varona, Luis“…These regions contain, amongst others, the following candidate genes: NCK1, MSTN, KCNA3, LCORL, NCAPG, and RIN3.…”
Publicado 2021
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1088por Schmitz, Rosa Lynn, Weissbach, Julia, Kleilein, Jan, Bell, Jessica, Hüttelmaier, Stefan, Viol, Fabrice, Clauditz, Till, Grabowski, Patricia, Laumen, Helmut, Rosendahl, Jonas, Michl, Patrick, Schrader, Jörg, Krug, Sebastian“…The primary human PanNET cells NT-3 and NT-18 as well as the murine insulinoma cell lines beta-TC-6 (mouse) and RIN-T3 (rat) were treated with the non-selective histone-deacetylase (HDAC) inhibitor panobinostat (PB) and analyzed for functional effects and affected signaling pathways by performing Western blot, FACS and qPCR analyses. …”
Publicado 2021
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1089por Meecham, Amelia, Miranda, Elena, Morris, Hayley T., Hair, Jane, Oien, Karin A., Gerrard, Gareth, Guppy, Naomi, Mooney, David, Shaw, Emily C., Ashton-Key, Margaret, Lees, Robert, Flanagan, Adrienne, Rodriguez-Justo, Manuel“…Quality of DNA and RNA was significantly improved by PAXgene tissue fixation [RIN 2.8 versus 3.8 (p < 0.01), DIN 5.68 versus 6.77 (p < 0.001)], which translated into improved performance on qPCR assay. …”
Publicado 2021
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1090por Wani, Umer Majeed, Wani, Zubair Ahmad, Koul, Aabid M., Amin, Asif, Shah, Basit Amin, Farooq, Faizah, Qadri, Raies A.“…Our method led to the isolation of clear and non-dispersive total RNA with an RNA Integrity Number (RIN) value greater than 7.5. The quality of the RNA was further assessed by qPCR-based amplification of mRNA and mature miRNAs such as Cs-MIR166c and Cs-MIR396a. …”
Publicado 2022
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1091por Bohan, Amy E., Purvis, Katelyn N., Sawyer, Jason T., Bergen, Werner G., Brandebourg, Terry D.“…Sampling time did not affect RNA quality, as determined by the RNA integrity number of RNA samples purified from either adipose (RIN; p > 0.54) or muscle tissue (p > 0.43). Likewise, the sampling time did not influence the results of real-time PCR analysis of gene expression when comparing RNA samples prepared from adipose or muscle tissue immediately following either exsanguination or scalding (p > 0.92). …”
Publicado 2022
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1092“…Moreover, FDR-significant effects from region analysis implicated genes with genetic variants previously associated with PDB severity, including RIN3 and CSF1. A multivariate predictor model featuring the top severity-associated CpG sites revealed a significant correlation (R = 0.71, p = 6.9 × 10(−16)) between observed and predicted PDB severity scores. …”
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1093por He, Yinli, Dong, Lele, Yi, Hongyang, Zhang, Linpei, Shi, Xue, Su, Lin, Gan, Baoyu, Guo, Ruirui, Wang, Yawen, Luo, Qinying, Li, Xiaojiao“…A novel enhanced evaluation system including RNA yields, purity, RNA integrity number (RIN) values and β-actin copies was employed to more sensitively identify RNA quality differences. …”
Publicado 2023
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1094por Velayudhan, Silpa Mullakkalparambil, Alam, Shahin, Yin, Tong, Brügemann, Kerstin, Buerkert, Andreas, Sejian, Veerasamy, Bhatta, Raghavendra, Schlecht, Eva, König, Sven“…Selection sweeps are located in chromosomal segments in close proximity to the genes lrand rab interactor 3 (RIN3), solute carrier family 24 member 4 (SLC24A4), tetraspanin 3 (TSPAN3), and proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1). …”
Publicado 2023
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1095Whole genome transcript profiling from fingerstick blood samples: a comparison and feasibility studypor Robison, Elizabeth H, Mondala, Tony S, Williams, Adam R, Head, Steven R, Salomon, Daniel R, Kurian, Sunil M“…RESULTS: From two sets of fingerstick collections, about 70 uL whole blood collected via finger lancet and capillary tube, we recovered an average of 252.6 ng total RNA with an average RIN of 9.3. The post-amplification yields for 50 ng of total RNA averaged at 7.0 ug cDNA. …”
Publicado 2009
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1096por de Cremoux, Patricia, Valet, Fabien, Gentien, David, Lehmann-Che, Jacqueline, Scott, Véronique, Tran-Perennou, Carine, Barbaroux, Catherine, Servant, Nicolas, Vacher, Sophie, Sigal-Zafrani, Brigitte, Mathieu, Marie-Christine, Bertheau, Philippe, Guinebretière, Jean-Marc, Asselain, Bernard, Marty, Michel, Spyratos, Frédérique“…RESULTS: Thirty seven samples were excluded because i) they contained less than 30% malignant cells, or ii) they provided RNA Integrity Number (RIN) of poor quality. Among the 290 remaining cases, taking into account strict quality control criteria initially defined to ensure good quality of sampling, 78% and 82% samples were eligible for transcriptome and RT-qPCR analyses, respectively. …”
Publicado 2011
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1097por Duale, Nur, Lipkin, W Ian, Briese, Thomas, Aarem, Jeanette, Rønningen, Kjersti S, Aas, Kaja K, Magnus, Per, Harbak, Kari, Susser, Ezra, Brunborg, Gunnar“…FINDINGS: The RNA purity and integrity, expressed as RIN-values, were stable up to six years of storage, and there were no storage-related deleterious effects on RNA purity. …”
Publicado 2014
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1098por Tatarkiewicz, K, Hargrove, D M, Jodka, C M, Gedulin, B R, Smith, P A, Hoyt, J A, Lwin, A, Collins, L, Mamedova, L, Levy, O E, D’Souza, L, Janssen, S, Srivastava, V, Ghosh, S S, Parkes, D G“…The functional activity and binding of AC163794 to the GIP receptor were evaluated in RIN-m5F β-cells. In vitro metabolic stability was tested in human plasma and kidney membrane preparations. …”
Publicado 2014
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1099por Reck, Michael, Tomasch, Jürgen, Deng, Zhiluo, Jarek, Michael, Husemann, Peter, Wagner-Döbler, Irene“…Next, the influence of the stabilization reagent during sample storage for up to 15 days was studied. RIN analysis and qRT-PCR of spiked-in and indigenous genes revealed that RNA Later preserved mRNA integrity most efficiently, while samples conserved in RNA Protect showed substantial mRNA decay. …”
Publicado 2015
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1100por Ho, Thai H., Nateras, Rafael Nunez, Yan, Huihuang, Park, Jin G., Jensen, Sally, Borges, Chad, Lee, Jeong Heon, Champion, Mia D., Tibes, Raoul, Bryce, Alan H., Carballido, Estrella M., Todd, Mark A., Joseph, Richard W., Wong, William W., Parker, Alexander S., Stanton, Melissa L., Castle, Erik P.“…Our mean (SD) TCGA RNA integrity numbers (RINs) were 8.1 (0.8) for papillary RCC, with a 12.5% overall rate of sample disqualification for RIN <7. …”
Publicado 2015
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