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Mostrando 21 - 40 Resultados de 9,396 Para Buscar '"Sanger"', tiempo de consulta: 0.48s Limitar resultados
  1. 21
    “…The precision and robustness of Sanger sequencing contribute significantly to the scientific basis of clinical investigations. …”
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    Mastering DNA chromatogram analysis in Sanger sequencing for reliable clinical analysis
  2. 22
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    Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches
  3. 23
    “…RESULTS: We developed a robust 8-amplicon Sanger sequencing strategy that regularly produces complete, forensic-quality mtGenome haplotypes in the first pass of data generation. …”
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    A high-throughput Sanger strategy for human mitochondrial genome sequencing
  4. 24
    “…The identity of Sanger generated sequences with published consensus NGS support the use of Sanger method for ZIKV population studies. …”
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    Simple protocol for population (Sanger) sequencing for Zika virus genomic regions
  5. 25
    “…Despite its long history, there is a lack of user-friendly Sanger sequencing data analysis tools that can be run interactively as a web application or at large-scale in batch from the command-line. …”
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    Tracy: basecalling, alignment, assembly and deconvolution of sanger chromatogram trace files
  6. 26
    “…sangeranalyseR is feature-rich, free, and open-source R package for processing Sanger sequencing data. It allows users to go from loading reads to saving aligned contigs in a few lines of R code by using sensible defaults for most actions. …”
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    sangeranalyseR: Simple and Interactive Processing of Sanger Sequencing Data in R
  7. 27
    “…Employing the algorithm in clinical practice reduced overall orthogonal testing using dideoxynucleotide (Sanger) sequencing by 71%. CONCLUSION: Our results indicate that a low false positive call rate can be maintained while significantly reducing the need for confirmatory testing. …”
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    Reducing Sanger confirmation testing through false positive prediction algorithms
  8. 28
    “…We describe a Sanger sequencing protocol for SARS‐CoV‐2 S‐gene the Spike (S)‐glycoprotein product of which, composed of receptor‐binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID‐19. …”
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    A Sanger sequencing protocol for SARS‐CoV‐2 S‐gene
  9. 29
    “…Thus, the three elemental Sanger-based DNA sequencing steps were accomplished within 41 min. …”
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    Rapid DNA Sequencing Technology Based on the Sanger Method for Bacterial Identification
  10. 30
    “…This antibody gene signature (AGS) was developed using Sanger sequencing of single B cells. While potentially helpful to patients, Sanger sequencing is not an assay that can be practically deployed in clinical settings. …”
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    The Antibody Genetics of Multiple Sclerosis: Comparing Next-Generation Sequencing to Sanger Sequencing
  11. 31
    “…The results were confirmed by Sanger sequencing and compared with results from fragment analysis. …”
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    Screening PCR Versus Sanger Sequencing: Detection of CALR Mutations in Patients With Thrombocytosis
  12. 32
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    ALTERNATIVE METHODS FOR SEQUENCING FULL TSPyV GENOMES USING SANGER OR NGS
  13. 33
    “…According to HTS data, Sanger technology consistently yielded the most abundant photobiont sequence in the sample. …”
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    Comparing Sanger sequencing and high-throughput metabarcoding for inferring photobiont diversity in lichens
  14. 34
    “…To overcome these limitations, we developed a simple, cost-effective, and accurate program to measure base editing efficiency from fluorescence-based Sanger sequencing, termed “EditR.” We provide EditR as a free online tool or downloadable desktop application requiring a single Sanger sequencing file and guide RNA sequence. …”
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    EditR: A Method to Quantify Base Editing from Sanger Sequencing
  15. 35
    “…The diagnostic yield of direct Sanger sequencing of CLN genes was 27% in symptomatic patients. …”
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    High diagnostic yield of direct Sanger sequencing in the diagnosis of neuronal ceroid lipofuscinoses
  16. 36
    “…The accuracy was compared with the results obtained with Sanger. RESULTS: We found that HipSTR was more accurate than Sanger in genotyping normal karyotype men (46,XY), however, it was more likely to misidentify homozygote genotypes in men with Klinefelter syndrome (47,XXY). …”
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    Comparison of NGS panel and Sanger sequencing for genotyping CAG repeats in the AR gene
  17. 37
    “…Variants called following guidelines suggested by the Broad Institute and identified according to MAF <0.01 and allele balance >0.2 were Sanger validated. Three out of 945 validated variants showed a discrepancy between NGS and Sanger. …”
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    Sanger Validation of High-Throughput Sequencing in Genetic Diagnosis: Still the Best Practice?
  18. 38
    “…Therefore, to support the urgent surveillance of SARS-CoV-2 S gene, this work describes a new feasible protocol for complete nucleotide sequencing of the S gene using the Sanger technique. Such a methodology could be easily adopted by any laboratory with experience in sequencing, adding to effective surveillance of SARS-CoV-2 spreading and evolution.…”
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    Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing
  19. 39
    “…[Image: see text] Facile synthesis of molecular hybrids containing a 2,4-dinitrophenyl moiety was achieved via nucleophilic aromatic substitution of the fluoride anion of Sanger’s reagent (2,4-dinitrofluorobenzene) with various N, S, and O nucleophiles, considered as bioactive moieties. …”
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    Exploration of Nitroaromatic Antibiotics via Sanger’s Reagent: Synthesis, In Silico, and Antimicrobial Evaluation
  20. 40
    “…Samples were evaluated immediately using parallel multiplex RT-qPCR and nanopore sequencing, and were then amplified by designed primers and Sanger sequencing to obtain whole genome sequences. …”
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    Characterisation of human astrovirus in a diarrhoea outbreak using nanopore and Sanger sequencing protocols
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