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Mostrando 561 - 580 Resultados de 9,396 Para Buscar '"Sanger"', tiempo de consulta: 0.17s Limitar resultados
  1. 561
    “…To assess the impact of methodology on the detectable diversity of the coral's microbiome, we obtained near full-length Sanger sequences from clone libraries constructed from a subset of the same A. poculata samples. …”
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    Stability of temperate coral Astrangia poculata microbiome is reflected across different sequencing methodologies
  2. 562
    “…Here, we developed a precise and rapid Sanger sequencing assay to screen or TERTp mutations. …”
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    Establishment and Validation of the Detection of TERT Promoter Mutations by Human Gliomas U251 Cell Lines
  3. 563
    “…A series of 71 patients including Usher patients previously screened by Sanger sequencing plus newly referred patients was studied. …”
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    Experience of targeted Usher exome sequencing as a clinical test
  4. 564
    “…However, the traditional MLST process using Sanger sequencing is time-consuming and expensive. …”
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    nanoMLST: accurate multilocus sequence typing using Oxford Nanopore Technologies MinION with a dual-barcode approach to multiplex large numbers of samples
  5. 565
    “…This review discusses use of Sanger sequencing and next generation sequencing (NGS) methods for HIV-1 drug resistance (HIVDR) genotyping, focusing on their use in resource limited settings (RLS). …”
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    HIV-1 Drug Resistance Genotyping in Resource Limited Settings: Current and Future Perspectives in Sequencing Technologies
  6. 566
    “…Details on the system requirements and installation of ProServer can be found at http://www.sanger.ac.uk/proserver/. Contact: rmp@sanger.ac.uk Supplementary Materials: DasClientExamples.pdf…”
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    ProServer: a simple, extensible Perl DAS server
  7. 567
    “…In addition, we found Precision ID to be more susceptible to inhibition than our legacy Sanger assay. Overall, the success rate (full single source hypervariable regions I and II (HVI/HVII) for Sanger and control region for MPS result) for these challenging samples increased from 32.3% with Sanger sequencing to 74.2% with the Precision ID assay. …”
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    Mitochondrial Sequencing of Missing Persons DNA Casework by Implementing Thermo Fisher’s Precision ID mtDNA Whole Genome Assay
  8. 568
    “…Evaluating the potential of Sanger sequencing to identify genetically resistant determinants in Helicobacter pylori clinical isolates will be important. …”
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    Direct Detection of Antibiotic Resistance in Chinese Helicobacter pylori Clinical Isolates by Sequencing-Based Approach
  9. 569
    “…The methods included a morphological evaluation by light microscopy, Sanger sequencing of PCR amplicons of COI and 18S gene regions, and 18S metabarcoding sequencing. …”
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    Plectus of the Prairie: A Case Study of Taxonomic Resolution from a Nematode Biodiversity Survey
  10. 570
    “…RESULTS: PPA between cobas and Sanger was 98.8%; NPA was 79.3%. Overall there were seven discordant results. …”
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    Comparison of molecular testing methods for the detection of EGFR mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer
  11. 571
    “…We found inconsistencies in the individual genotypes between the two methods, which were mainly caused by allelic dropout in the SSCP/Sanger method. Six out of 28 individuals were falsely classified as homozygous with SSCP/Sanger analysis. …”
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    MHC Genotyping by SSCP and Amplicon-Based NGS Approach in Chamois
  12. 572
    “…However, only the commercial software, such as uTYPE, SBT-Assign, and SBTEngine, and very few open-source tools could be applied to perform HLA typing based on Sanger sequencing. RESULTS: We developed a user-friendly, cross-platform and open-source desktop application, known as SOAPTyping, for Sanger-based typing in HLA class I and II alleles. …”
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    SOAPTyping: an open-source and cross-platform tool for sequence-based typing for HLA class I and II alleles
  13. 573
    “…The findings of NGS were confirmed by Sanger sequencing. When possible, genetic analyses of the new findings in the proband's relatives were also performed by Sanger sequencing. …”
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    Co‐occurrence of germline pathogenic variants for different hereditary cancer syndromes in patients with Lynch syndrome
  14. 574
    “…RESULTS: one hundred and twenty samples previously characterized by Sanger sequencing were assessed using PANDAA. PDR was found in 14% (17/120). …”
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    Rapid HIV-1 drug resistance testing in a resource limited setting: the Pan Degenerate Amplification and Adaptation assay (PANDAA)
  15. 575
    “…Nevertheless, traditional Sanger sequencing is laborious, time-consuming, and low throughput. …”
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    The Clinical Utility of Two High-Throughput 16S rRNA Gene Sequencing Workflows for Taxonomic Assignment of Unidentifiable Bacterial Pathogens in Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
  16. 576
    “…ABSTRACT: Despite several decades’ effort to detect and identify phytoplasmas (Mollicutes) using PCR and Sanger sequencing focusing on diseased plants, knowledge of phytoplasma biodiversity and vector associations remains highly incomplete. …”
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    Comparison of Traditional and Next-Generation Approaches for Uncovering Phytoplasma Diversity, with Discovery of New Groups, Subgroups and Potential Vectors
  17. 577
    “…The training cohort and the clinical validation cohort showed equally, or significantly better sensitivity of the assays compared to the Sanger sequencing. The agreement of the Sanger sequencing and the assays ranged from 92.6 to 100% for the training cohort and 99.4–100% for the clinical validation. …”
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    Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants
  18. 578
    por Okumura, Shiro, Ohsato, Yoshiharu
    Publicado 2023
    “…We postulate that the Sanger method gave a negative result because of the low abundance of the mutated gene in these samples, proving the high sensitivity of our new method.…”
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    A novel rapid detection method for a single-nucleotide substitution mutation derived from canine urothelial and prostatic carcinoma cells present in small amounts in urine sediments
  19. 579
    “…DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. …”
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    Pyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens
  20. 580
    “…HRM and SSCA followed by Sanger sequencing are effective two-step strategies for the detection of BRAF mutations in the clinical setting. …”
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    A multisite blinded study for the detection of BRAF mutations in formalin-fixed, paraffin-embedded malignant melanoma
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